LOCUS       NM_133196               4542 bp    mRNA    linear   ROD 10-FEB-2025
DEFINITION  Mus musculus cleavage stimulation factor, 3' pre-RNA subunit 2
            (Cstf2), transcript variant 1, mRNA.
ACCESSION   NM_133196
VERSION     NM_133196.6
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4542)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 4542)
  AUTHORS   Bedogni,F. and Hevner,R.F.
  TITLE     Cell-Type-Specific Gene Expression in Developing Mouse Neocortex:
            Intermediate Progenitors Implicated in Axon Development
  JOURNAL   Front Mol Neurosci 14, 686034 (2021)
   PUBMED   34321999
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 4542)
  AUTHORS   Hildebrandt,M.R., Wang,Y., Li,L., Yasmin,L., Glubrecht,D.D. and
            Godbout,R.
  TITLE     Cytoplasmic aggregation of DDX1 in developing embryos: Early
            embryonic lethality associated with Ddx1 knockout
  JOURNAL   Dev Biol 455 (2), 420-433 (2019)
   PUBMED   31330130
REFERENCE   4  (bases 1 to 4542)
  AUTHORS   Nazim,M., Masuda,A., Rahman,M.A., Nasrin,F., Takeda,J.I., Ohe,K.,
            Ohkawara,B., Ito,M. and Ohno,K.
  TITLE     Competitive regulation of alternative splicing and alternative
            polyadenylation by hnRNP H and CstF64 determines
            acetylcholinesterase isoforms
  JOURNAL   Nucleic Acids Res 45 (3), 1455-1468 (2017)
   PUBMED   28180311
REFERENCE   5  (bases 1 to 4542)
  AUTHORS   Youngblood,B.A. and MacDonald,C.C.
  TITLE     CstF-64 is necessary for endoderm differentiation resulting in
            cardiomyocyte defects
  JOURNAL   Stem Cell Res 13 (3 Pt A), 413-421 (2014)
   PUBMED   25460602
REFERENCE   6  (bases 1 to 4542)
  AUTHORS   Wallace,A.M., Denison,T.L., Attaya,E.N. and MacDonald,C.C.
  TITLE     Developmental distribution of the polyadenylation protein CstF-64
            and the variant tauCstF-64 in mouse and rat testis
  JOURNAL   Biol Reprod 70 (4), 1080-1087 (2004)
   PUBMED   14681198
REFERENCE   7  (bases 1 to 4542)
  AUTHORS   Dass,B., Attaya,E.N., Michelle Wallace,A. and MacDonald,C.C.
  TITLE     Overexpression of the CstF-64 and CPSF-160 polyadenylation protein
            messenger RNAs in mouse male germ cells
  JOURNAL   Biol Reprod 64 (6), 1722-1729 (2001)
   PUBMED   11369601
REFERENCE   8  (bases 1 to 4542)
  AUTHORS   Wallace,A.M., Dass,B., Ravnik,S.E., Tonk,V., Jenkins,N.A.,
            Gilbert,D.J., Copeland,N.G. and MacDonald,C.C.
  TITLE     Two distinct forms of the 64,000 Mr protein of the cleavage
            stimulation factor are expressed in mouse male germ cells
  JOURNAL   Proc Natl Acad Sci U S A 96 (12), 6763-6768 (1999)
   PUBMED   10359786
REFERENCE   9  (bases 1 to 4542)
  AUTHORS   Martincic,K., Campbell,R., Edwalds-Gilbert,G., Souan,L., Lotze,M.T.
            and Milcarek,C.
  TITLE     Increase in the 64-kDa subunit of the polyadenylation/cleavage
            stimulatory factor during the G0 to S phase transition
  JOURNAL   Proc Natl Acad Sci U S A 95 (19), 11095-11100 (1998)
   PUBMED   9736695
REFERENCE   10 (bases 1 to 4542)
  AUTHORS   Takagaki,Y., Seipelt,R.L., Peterson,M.L. and Manley,J.L.
  TITLE     The polyadenylation factor CstF-64 regulates alternative processing
            of IgM heavy chain pre-mRNA during B cell differentiation
  JOURNAL   Cell 87 (5), 941-952 (1996)
   PUBMED   8945520
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CJ183629.1, AK032817.1, AL671915.8, AK147438.1 and AI850965.1.
            
            On Mar 13, 2014 this sequence version replaced NM_133196.5.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK088260.1, SRR7652917.40513.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-21                CJ183629.1         1-21
            22-1083             AK032817.1         4-1065
            1084-1084           AL671915.8         37221-37221
            1085-2400           AK032817.1         1067-2382
            2401-4523           AK147438.1         5395-7517
            4524-4542           AI850965.1         1-19                c
FEATURES             Location/Qualifiers
     source          1..4542
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10090"
                     /chromosome="X"
                     /map="X 55.63 cM"
     gene            1..4542
                     /gene="Cstf2"
                     /gene_synonym="C630034J23Rik; Cstf64; mir-3112"
                     /note="cleavage stimulation factor, 3' pre-RNA subunit 2"
                     /db_xref="GeneID:108062"
                     /db_xref="MGI:MGI:1343054"
     exon            1..247
                     /gene="Cstf2"
                     /gene_synonym="C630034J23Rik; Cstf64; mir-3112"
                     /inference="alignment:Splign:2.1.0"
     CDS             190..1932
                     /gene="Cstf2"
                     /gene_synonym="C630034J23Rik; Cstf64; mir-3112"
                     /note="isoform 1 is encoded by transcript variant 1;
                     cleavage stimulation factor subunit 2; CF-1 64 kDa
                     subunit; CSTF 64 kDa subunit; cleavage stimulation factor,
                     3' pre-RNA subunit 2, 64 kDa"
                     /codon_start=1
                     /product="cleavage stimulation factor subunit 2 isoform 1"
                     /protein_id="NP_573459.1"
                     /db_xref="CCDS:CCDS30389.1"
                     /db_xref="GeneID:108062"
                     /db_xref="MGI:MGI:1343054"
                     /translation="MAGLPVRDPAVDRSLRSVFVGNIPYEATEEQLKDIFSEVGPVVS
                     FRLVYDRETGKPKGYGFCEYQDQETALSAMRNLNGREFSGRALRVDNAASEKNKEELK
                     SLGTGAPVIESPYGESISPEDAPESISKAVASLPPEQMFELMKQMKLCVQNSPQEARN
                     MLLQNPQLAYALLQAQVVMRIVDPEIALKILHRQTNIPTLISGNPQPVHVAGPGSGPN
                     VSMNQQNPQAPQAQSLGGMHVNGAPPMMQASMPGGVPAPVQMAAAVGGPGPGSLAPAG
                     VMQAQVGMQGAGPVPMERGQVPMQDPRAAMQRGALPTNVPTPRGLLGDAPNDPRGGTL
                     MTVTGDVEPRAYLGPPPPPHQGPPMHHVPGHEGRGPPPHDMRGGPLAEPRPLMAEPRG
                     PMLDQRGPPLDARGGRDPRGLDARGMEARAMEARGLDARGLEARAMEARAMEARAMEA
                     RAMEARAMEARAMEARGMDTRGPVPGPRGPMPSGIQGPNPMNMGAVVPQGSRQVPVMQ
                     GAGMQGASMQGGSQPGGFSPGQSQVTPQDHEKAALIMQVLQLTADQIAMLPPEQRQSI
                     LILKEQIQKSTGAP"
     exon            248..326
                     /gene="Cstf2"
                     /gene_synonym="C630034J23Rik; Cstf64; mir-3112"
                     /inference="alignment:Splign:2.1.0"
     exon            327..496
                     /gene="Cstf2"
                     /gene_synonym="C630034J23Rik; Cstf64; mir-3112"
                     /inference="alignment:Splign:2.1.0"
     exon            497..633
                     /gene="Cstf2"
                     /gene_synonym="C630034J23Rik; Cstf64; mir-3112"
                     /inference="alignment:Splign:2.1.0"
     exon            634..753
                     /gene="Cstf2"
                     /gene_synonym="C630034J23Rik; Cstf64; mir-3112"
                     /inference="alignment:Splign:2.1.0"
     exon            754..891
                     /gene="Cstf2"
                     /gene_synonym="C630034J23Rik; Cstf64; mir-3112"
                     /inference="alignment:Splign:2.1.0"
     exon            892..1015
                     /gene="Cstf2"
                     /gene_synonym="C630034J23Rik; Cstf64; mir-3112"
                     /inference="alignment:Splign:2.1.0"
     exon            1016..1078
                     /gene="Cstf2"
                     /gene_synonym="C630034J23Rik; Cstf64; mir-3112"
                     /inference="alignment:Splign:2.1.0"
     exon            1079..1220
                     /gene="Cstf2"
                     /gene_synonym="C630034J23Rik; Cstf64; mir-3112"
                     /inference="alignment:Splign:2.1.0"
     exon            1221..1405
                     /gene="Cstf2"
                     /gene_synonym="C630034J23Rik; Cstf64; mir-3112"
                     /inference="alignment:Splign:2.1.0"
     exon            1406..1698
                     /gene="Cstf2"
                     /gene_synonym="C630034J23Rik; Cstf64; mir-3112"
                     /inference="alignment:Splign:2.1.0"
     exon            1699..1809
                     /gene="Cstf2"
                     /gene_synonym="C630034J23Rik; Cstf64; mir-3112"
                     /inference="alignment:Splign:2.1.0"
     exon            1810..1935
                     /gene="Cstf2"
                     /gene_synonym="C630034J23Rik; Cstf64; mir-3112"
                     /inference="alignment:Splign:2.1.0"
     exon            1936..4526
                     /gene="Cstf2"
                     /gene_synonym="C630034J23Rik; Cstf64; mir-3112"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2131..2136
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Cstf2"
                     /gene_synonym="C630034J23Rik; Cstf64; mir-3112"
                     /note="hexamer: AATAAA"
     polyA_site      2161
                     /gene="Cstf2"
                     /gene_synonym="C630034J23Rik; Cstf64; mir-3112"
     regulatory      4507..4512
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Cstf2"
                     /gene_synonym="C630034J23Rik; Cstf64; mir-3112"
                     /note="hexamer: AATAAA"
     polyA_site      4526
                     /gene="Cstf2"
                     /gene_synonym="C630034J23Rik; Cstf64; mir-3112"
                     /note="major polyA site"
ORIGIN      
        1 tgttgcccct cccccggggc cgagccgcct ggcgggaacg accgcggtga ggcgcgcggc
       61 cgggctccgc ggcggcgctt cggggccagg tgcttccggc tcccaccgga cggaggcggg
      121 ctgggccgtg gtctcgctcg gccgcggaag tgtgctttag cgcaccggaa gccacctcgg
      181 gggtgagcca tggcgggttt gccagtgaga gaccctgcgg tggatcgttc cctgcgctcc
      241 gtcttcgtgg gaaacatccc ttatgaagct actgaagaac aactgaagga cattttctct
      301 gaggttgggc ctgttgttag tttcagattg gtatacgata gggagacagg aaaaccaaag
      361 ggctatggct tctgcgagta ccaagaccaa gagacagcac ttagtgccat gcggaacttg
      421 aatgggcgtg aattcagcgg gagagcactt cgagtggata atgctgccag tgaaaagaac
      481 aaggaggaac tgaagagcct tggcactggt gcacctgtca tagagtcacc ttatggagaa
      541 agcatcagcc ctgaggatgc ccctgaatcc attagcaaag cagttgccag tcttccacca
      601 gaacagatgt ttgagttgat gaaacaaatg aagctctgtg tccagaatag tccccaggaa
      661 gcacgaaaca tgttgcttca gaatccacag ctggcttatg ctttgttgca agcacaggta
      721 gtgatgagaa ttgtggatcc tgagattgcg ctgaaaattc tgcatcgcca gacaaatatc
      781 ccaacgctga tttcaggcaa ccctcagcca gttcatgttg ctgggcctgg ctcaggaccc
      841 aatgtttcaa tgaaccagca gaatccgcag gcccctcagg ctcaatcttt gggtggaatg
      901 catgtcaatg gcgcacctcc tatgatgcaa gcttctatgc caggtggagt tccagcccca
      961 gtgcagatgg cagctgctgt aggaggacct ggccctggtt ccttagcccc tgcaggagtt
     1021 atgcaagccc aagttggaat gcaaggagct ggaccagtgc ccatggaacg aggacaagtg
     1081 ccaatgcaag accccagagc agctatgcag cggggagcct tgcctaccaa cgtcccaact
     1141 cctcgtggtc ttttaggtga tgctcccaat gacccgcggg gaggcacttt aatgactgta
     1201 actggagacg tagaacctag agcttacctg ggaccaccac caccacctca tcagggccca
     1261 cccatgcacc atgttcctgg tcatgaaggt cgtggaccgc ctccacatga catgagagga
     1321 ggacccctag ctgagccgag acctttaatg gcagaaccaa gaggacccat gctagatcag
     1381 aggggcccac ctctggatgc cagaggtgga agagatcccc gaggattaga tgcacggggg
     1441 atggaggcca gagccatgga agcaagagga ctagatgcca gaggactgga agcccgtgcc
     1501 atggaggccc gtgccatgga ggcccgtgcc atggaagccc gtgccatgga agcccgtgcc
     1561 atggaggctc gtgccatgga ggccagaggc atggacacca gaggcccagt acctggacct
     1621 agaggaccta tgcctagtgg aatccaaggt cccaatccaa tgaacatggg ggcagttgtt
     1681 ccccagggat ccagacaggt accagtcatg cagggagcag gcatgcaagg agcaagcatg
     1741 caaggtggaa gccagcctgg tggctttagt cctggacaga gccaagtcac accccaggat
     1801 catgagaagg ctgctttgat tatgcaggtc cttcagctca ctgcagacca gatcgccatg
     1861 ctgcctcctg agcaaagaca gagtatccta atcttaaagg aacagataca gaaatccact
     1921 ggggcacctt gaaaggttct caaaatagct ggcaacaaat ctggaaatgt aattccataa
     1981 catttttgaa attttgaaaa cataatgacg tctgcatccg aacctccccg gatgaggcag
     2041 atggcgccag gtggaggtct tacatcgcca ttgctccaaa ggaaagcagt ttattctgtt
     2101 gtcgtagtgt gtatattttc tgtgacatta aataaacttt gaacacaagt ttaacatact
     2161 acaaattgat atacataatc cttttgcttt taagaagcta acagcaagga tgtaagctta
     2221 actgtgattt ctaactgcag catcattctt taatcttaca ttgccatagc tctattctta
     2281 ctttagcata agcatttatt cttcttaatg atacttgaga atttctcaga aacaatgttt
     2341 tcaggaactt tgatttttgt tgaatttgct ttcagtaaag taataacact ttaccccgtg
     2401 aaaactttct agagtctcag agctttctgg gaggaaaaca aaaaagcacg tggttagaac
     2461 taaaaatgat cagaaggcca acaagggaag agcgtaccac aacagtgtgt ttgcttccag
     2521 aaatgccagt tatccaagtg tgttgtttgg tcagtagcag gtgtgaacat acataggaca
     2581 aaggctgtcg tggcactggt ttgcaaaaga ttgtcgtaaa gctctcctgt ctaggtagcc
     2641 cgctttacaa gtttaaaaag gcactggttt tccacatggt caaatttaat aaagaaaacc
     2701 aagtgaacaa aatatattcc aatctttcag aaaatcacaa agctttcagc cctgtcatct
     2761 taactaaatc cattccaaga acttcaaaca gaaaacccta aaactttgac attttcaagt
     2821 ctttaaagag gaccaagtat agagcaccgg acggcaaact gcccgttatg gttgcctgct
     2881 tctttccaag tccgaccaac ttggaaggca attagtgcca ttagcctgtg ctaacctgca
     2941 ggttgccttg ccttttgttc tcccacagtg gcattaagga ggaacttggt ttctctccac
     3001 cagtttgacc tcaagacatt tgaagagttg ccttaataat tattaagttt actagcacta
     3061 attgtaagct tatttgtatt cccagatccc taccctctgc attaggatta tgggcaggtg
     3121 aattaaaatc actacttaag gcataaaaag catagtagta cattgcttat atgccaagaa
     3181 aaagaaagca aagatattaa aattgaagga tttttctcca tatattttta atgaaaattt
     3241 gctggatctg tcatattata acctgtcaag aaaagtataa aacaacaagc actcctgcct
     3301 gcctattcca ctcatgccca gttcaacccc gatggatgtt aattatgcat gcacttcttt
     3361 gtcattaccc tctgtgcctc ctgcagaagc agtccttgct gtgttggttt tggcagctgt
     3421 cttgacttaa aagcaccaat gtatttagat gcagagacca agagcaaagt atttctagtt
     3481 aggaacttgg gttttacaca accagttcca aatcaagata agtgaagctg aagtgatttg
     3541 ggaaacagtt gtctagtaga aaccatgtcc tggaaagcca gcacattttc ccgaagcctc
     3601 agtaaactta atttgcttag caggcattca ggtgactaag ttgacaagag cagtggcatc
     3661 ccaattcatc actatggaaa cactcatcag tggttggttg gtttgtttgc tgttattttt
     3721 tactgttgta ataatacagt tagctacccc acgttcatga tctgccttta ttatgttcaa
     3781 aactaatgac tgaaaaataa tcccacaaat ccttcaggct gtaagttcct agataattga
     3841 aaacaaaaat gtgttcactt ttggttaatt aatggtacaa attagatggt ctagtgctaa
     3901 ctctatagat tcattctgct aaatgtatga ggtcaactgc ctgagtacag tgaagtgtgg
     3961 agttttttct ccctgaagag cctgcacctt tttgtgtaat tcaccataac taacatccca
     4021 gagccagcgt gcttattgtc ctggttgata ggaaaaatca acatttttgt aggaaaaaga
     4081 gtgacattta gtggatgatt cctgattgag taagacctgg aaaatagcca aaattataac
     4141 ttttttcttt ttataataca ttcattaaca aactccttta acaacaacaa aaagcataaa
     4201 agagcttcag ttcaattatc tcatgatgta cgtcaggcct ttccagttat tccaaacaat
     4261 acaaagtgac aatatagttg gcttctaact atagtacaaa tttgattaca gttaatatac
     4321 ttaagatatt actaggaaat tgttcaataa gcaaaaagga aagtcaatta ttttgacaaa
     4381 gtaacgtgta aattgttggt cacactggat taactcatgt aacaatgtac ttagtgggtg
     4441 atatgggagt gggaagcttg gtcgttctat gttgtttatt tatgattgtt gttctgtcgt
     4501 ggattcaata aatattttaa aagtcaaaaa aaaaaaaaaa aa
//
