LOCUS       NM_009978                703 bp    mRNA    linear   ROD 01-JUN-2024
DEFINITION  Mus musculus cystatin 8 (cystatin-related epididymal spermatogenic)
            (Cst8), mRNA.
ACCESSION   NM_009978
VERSION     NM_009978.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 703)
  AUTHORS   Do,H.Q., Hewetson,A., Borcik,C.G., Hastert,M.C., Whelly,S.,
            Wylie,B.J., Sutton,R.B. and Cornwall,G.A.
  TITLE     Cross-seeding between the functional amyloidogenic CRES and CRES3
            family members and their regulation of Abeta assembly
  JOURNAL   J Biol Chem 296, 100250 (2021)
   PUBMED   33384380
  REMARK    GeneRIF: Cross-seeding between the functional amyloidogenic CRES
            and CRES3 family members and their regulation of Abeta assembly.
REFERENCE   2  (bases 1 to 703)
  AUTHORS   Hewetson,A., Khan,N.H., Dominguez,M.J., Do,H.Q., Kusko,R.E.,
            Borcik,C.G., Rigden,D.J., Keegan,R.M., Sutton,R.B., Latham,M.P.,
            Wylie,B.J. and Cornwall,G.A.
  TITLE     Maturation of the functional mouse CRES amyloid from globular form
  JOURNAL   Proc Natl Acad Sci U S A 117 (28), 16363-16372 (2020)
   PUBMED   32601205
  REMARK    GeneRIF: Maturation of the functional mouse CRES amyloid from
            globular form.
REFERENCE   3  (bases 1 to 703)
  AUTHORS   Ferrer,M., Cornwall,G. and Oko,R.
  TITLE     A population of CRES resides in the outer dense fibers of
            spermatozoa
  JOURNAL   Biol Reprod 88 (3), 65 (2013)
   PUBMED   23269664
  REMARK    GeneRIF: A 14-kDa CRES isoform resides in the outer dense fibers of
            the sperm tail.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 703)
  AUTHORS   Mishra,P., Qiu,Q., Gruslin,A., Hidaka,Y., Mbikay,M. and Basak,A.
  TITLE     In vitro regulatory effect of epididymal serpin CRES on protease
            activity of proprotein convertase PC4/PCSK4
  JOURNAL   Curr Mol Med 12 (8), 1050-1067 (2012)
   PUBMED   22827436
  REMARK    GeneRIF: CRES may be a regulator of PC4 in sperm-egg interaction
            and fertilization.
REFERENCE   5  (bases 1 to 703)
  AUTHORS   Wang,L., Yuan,Q., Chen,S., Cai,H., Lu,M., Liu,Y. and Xu,C.
  TITLE     Antimicrobial activity and molecular mechanism of the CRES protein
  JOURNAL   PLoS One 7 (11), e48368 (2012)
   PUBMED   23185254
  REMARK    GeneRIF: data on the antimicrobial activities of CRES protein
            suggest that it is a novel and innate antimicrobial protein which
            protecting the male reproductive tract against invading pathogens
REFERENCE   6  (bases 1 to 703)
  AUTHORS   Cornwall,G.A. and Hsia,N.
  TITLE     [Cres (cystatin-related epididymal spermatogenic) gene regulation
            and function]
  JOURNAL   Zhonghua Nan Ke Xue 8 (5), 313-318 (2002)
   PUBMED   12479114
  REMARK    GeneRIF: CRES is a new cross-class inhibitor that may regulate PC2
            of PC2-like proteases and suggests a role for CRES in the
            regulation of prohormone and proprotein processing.
            Review article
REFERENCE   7  (bases 1 to 703)
  AUTHORS   Cornwall,G.A., Hsia,N. and Sutton,H.G.
  TITLE     Structure, alternative splicing and chromosomal localization of the
            cystatin-related epididymal spermatogenic gene
  JOURNAL   Biochem J 340 (Pt 1) (Pt 1), 85-93 (1999)
   PUBMED   10229662
REFERENCE   8  (bases 1 to 703)
  AUTHORS   Tohonen,V., Osterlund,C. and Nordqvist,K.
  TITLE     Testatin: a cystatin-related gene expressed during early testis
            development
  JOURNAL   Proc Natl Acad Sci U S A 95 (24), 14208-14213 (1998)
   PUBMED   9826679
REFERENCE   9  (bases 1 to 703)
  AUTHORS   Cornwall,G.A. and Hann,S.R.
  TITLE     Transient appearance of CRES protein during spermatogenesis and
            caput epididymal sperm maturation
  JOURNAL   Mol Reprod Dev 41 (1), 37-46 (1995)
   PUBMED   7619504
REFERENCE   10 (bases 1 to 703)
  AUTHORS   Cornwall,G.A., Orgebin-Crist,M.C. and Hann,S.R.
  TITLE     The CRES gene: a unique testis-regulated gene related to the
            cystatin family is highly restricted in its expression to the
            proximal region of the mouse epididymis
  JOURNAL   Mol Endocrinol 6 (10), 1653-1664 (1992)
   PUBMED   1280328
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from AK018943.1 and
            S49926.1.
            
            On Apr 6, 2007 this sequence version replaced NM_009978.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK018943.1, BY707223.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849379, SAMN00849381
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-703               AK018943.1         2-704
FEATURES             Location/Qualifiers
     source          1..703
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 73.56 cM"
     gene            1..703
                     /gene="Cst8"
                     /gene_synonym="Cres; Cst-rs1"
                     /note="cystatin 8 (cystatin-related epididymal
                     spermatogenic)"
                     /db_xref="GeneID:13012"
                     /db_xref="MGI:MGI:107161"
     exon            1..93
                     /gene="Cst8"
                     /gene_synonym="Cres; Cst-rs1"
                     /inference="alignment:Splign:2.1.0"
     exon            94..421
                     /gene="Cst8"
                     /gene_synonym="Cres; Cst-rs1"
                     /inference="alignment:Splign:2.1.0"
     CDS             191..619
                     /gene="Cst8"
                     /gene_synonym="Cres; Cst-rs1"
                     /note="cystatin-8; cystatin-related epididymal-specific
                     protein; cystatin-related epididymal spermatogenic
                     protein"
                     /codon_start=1
                     /product="cystatin-8 precursor"
                     /protein_id="NP_034108.1"
                     /db_xref="CCDS:CCDS16848.1"
                     /db_xref="GeneID:13012"
                     /db_xref="MGI:MGI:107161"
                     /translation="MAKPLWLSLILFIIPVALAVGVDQSKNEVKAQNYFGSINISNAN
                     VKQCVWFAMKEYNKESEDKYVFLVDKILHAKLQITDRMEYQIDVQISRSNCKKPLNNT
                     ENCIPQKKPELEKKMSCSFLVGALPWNGEFNLLSKECKDV"
     exon            422..535
                     /gene="Cst8"
                     /gene_synonym="Cres; Cst-rs1"
                     /inference="alignment:Splign:2.1.0"
     exon            536..703
                     /gene="Cst8"
                     /gene_synonym="Cres; Cst-rs1"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gagagtgtgg gaaacccaga agaaccacaa gctggggtgg ccagagagag gaaggagccc
       61 agcaggacga gcaggtggga gagtcacaga aagttcgaca gacctacaga ggcagcagtg
      121 actgctactc tgaagctcaa gccccgtgga aacatagagg acatcttttg taccttttga
      181 ctgagggacc atggcaaaac ccttgtggct ctccttgatc ctcttcatca ttccagtggc
      241 cctggcagtt ggtgtggatc agtccaagaa tgaagtgaag gcacagaact actttggatc
      301 cattaatatc tctaatgcca atgtgaagca gtgtgtttgg tttgccatga aagaatacaa
      361 caaggaaagt gaggacaaat atgtcttcct tgtggacaag atactccatg ccaaacttca
      421 gatcacagac cgaatggaat accaaattga tgttcagatc tcccgcagta attgcaaaaa
      481 acctttaaac aatactgaaa actgcatccc tcaaaaaaaa cccgaactgg aaaaaaaaat
      541 gagttgcagc tttctggtag gagcacttcc ctggaatggc gagttcaacc tgttgagcaa
      601 agaatgtaaa gatgtctaag ggtgtcttgg agcacccctc ccacttctgg gtctgctttt
      661 ctctgtaata aagagcaacc tgtggttcaa gtctgtcacc aac
//
