LOCUS       NM_009977                986 bp    mRNA    linear   ROD 15-OCT-2024
DEFINITION  Mus musculus cystatin F (leukocystatin) (Cst7), mRNA.
ACCESSION   NM_009977
VERSION     NM_009977.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 986)
  AUTHORS   Syage,A.R., Pachow,C., Murray,K.M., Henningfield,C., Fernandez,K.,
            Du,A., Cheng,Y., Olivarria,G., Kawauchi,S., MacGregor,G.R.,
            Green,K.N. and Lane,T.E.
  TITLE     Cystatin F attenuates neuroinflammation and demyelination following
            murine coronavirus infection of the central nervous system
  JOURNAL   J Neuroinflammation 21 (1), 157 (2024)
   PUBMED   38879499
  REMARK    GeneRIF: Cystatin F attenuates neuroinflammation and demyelination
            following murine coronavirus infection of the central nervous
            system.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 986)
  AUTHORS   Li,Q., Li,B., Liu,L., Wang,K.J., Liu,M.Y., Deng,Y., Li,Z.,
            Zhao,W.D., Wu,L.Y., Chen,Y.H. and Zhang,K.
  TITLE     Monocytes release cystatin F dimer to associate with Abeta and
            aggravate amyloid pathology and cognitive deficits in Alzheimer's
            disease
  JOURNAL   J Neuroinflammation 21 (1), 125 (2024)
   PUBMED   38730470
  REMARK    GeneRIF: Monocytes release cystatin F dimer to associate with Abeta
            and aggravate amyloid pathology and cognitive deficits in
            Alzheimer's disease.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 986)
  AUTHORS   Daniels,M.J.D., Lefevre,L., Szymkowiak,S., Drake,A., McCulloch,L.,
            Tzioras,M., Barrington,J., Dando,O.R., He,X., Mohammad,M.,
            Sasaguri,H., Saito,T., Saido,T.C., Spires-Jones,T.L. and
            McColl,B.W.
  TITLE     Cystatin F (Cst7) drives sex-dependent changes in microglia in an
            amyloid-driven model of Alzheimer's disease
  JOURNAL   Elife 12, e85279 (2023)
   PUBMED   38085657
  REMARK    GeneRIF: Cystatin F (Cst7) drives sex-dependent changes in
            microglia in an amyloid-driven model of Alzheimer's disease.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 986)
  AUTHORS   Li,J., Durose,W.W., Ito,J., Kakita,A., Iguchi,Y., Katsuno,M.,
            Kunisawa,K., Shimizu,T. and Ikenaka,K.
  TITLE     Exploring the factors underlying remyelination arrest by studying
            the post-transcriptional regulatory mechanisms of cystatin F gene
  JOURNAL   J Neurochem 157 (6), 2070-2090 (2021)
   PUBMED   32947653
  REMARK    GeneRIF: Exploring the factors underlying remyelination arrest by
            studying the post-transcriptional regulatory mechanisms of cystatin
            F gene.
REFERENCE   5  (bases 1 to 986)
  AUTHORS   Duan,W., Wang,H., Fan,Q., Chen,L., Huang,H. and Ran,H.
  TITLE     Cystatin F involvement in adenosine A2A receptor-mediated
            neuroinflammation in BV2 microglial cells
  JOURNAL   Sci Rep 8 (1), 6820 (2018)
   PUBMED   29717153
  REMARK    GeneRIF: These results indicate that hypoxia BV2 cells highly
            express Cystatin F, which is involved in A2A R activation-mediated
            neuroinflammation via the PKA/CREB and PKC/CREB or ERK1/2 signaling
            pathways.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 986)
  AUTHORS   Hamilton,G., Colbert,J.D., Schuettelkopf,A.W. and Watts,C.
  TITLE     Cystatin F is a cathepsin C-directed protease inhibitor regulated
            by proteolysis
  JOURNAL   EMBO J 27 (3), 499-508 (2008)
   PUBMED   18256700
REFERENCE   7  (bases 1 to 986)
  AUTHORS   Cappello,F., Gatti,E., Camossetto,V., David,A., Lelouard,H. and
            Pierre,P.
  TITLE     Cystatin F is secreted, but artificial modification of its
            C-terminus can induce its endocytic targeting
  JOURNAL   Exp Cell Res 297 (2), 607-618 (2004)
   PUBMED   15212960
REFERENCE   8  (bases 1 to 986)
  AUTHORS   Morita,M., Hara,Y., Tamai,Y., Arakawa,H. and Nishimura,S.
  TITLE     Genomic construct and mapping of the gene for CMAP
            (leukocystatin/cystatin F, CST7) and identification of a proximal
            novel gene, BSCv (C20orf3)
  JOURNAL   Genomics 67 (1), 87-91 (2000)
   PUBMED   10945474
REFERENCE   9  (bases 1 to 986)
  AUTHORS   Morita,M., Yoshiuchi,N., Arakawa,H. and Nishimura,S.
  TITLE     CMAP: a novel cystatin-like gene involved in liver metastasis
  JOURNAL   Cancer Res 59 (1), 151-158 (1999)
   PUBMED   9892200
REFERENCE   10 (bases 1 to 986)
  AUTHORS   Halfon,S., Ford,J., Foster,J., Dowling,L., Lucian,L., Sterling,M.,
            Xu,Y., Weiss,M., Ikeda,M., Liggett,D., Helms,A., Caux,C.,
            Lebecque,S., Hannum,C., Menon,S., McClanahan,T., Gorman,D. and
            Zurawski,G.
  TITLE     Leukocystatin, a new Class II cystatin expressed selectively by
            hematopoietic cells
  JOURNAL   J Biol Chem 273 (26), 16400-16408 (1998)
   PUBMED   9632704
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK004420.1 and BX630680.1.
            
            On Oct 31, 2009 this sequence version replaced NM_009977.2.
            
            Sequence Note: An in-frame AUG is located 23 codons upstream of the
            annotated translation start site but is not being annotated as a
            start site since it is not conserved and would disrupt the putative
            N-terminal signal peptide.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK004420.1, AB015224.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849375, SAMN00849377
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            CDS uses downstream in-frame AUG :: downstream AUG is associated
                                                with N-terminal localization
                                                signal
            RefSeq Select criteria           :: based on single protein-coding
                                                transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-978               AK004420.1         2-979
            979-986             BX630680.1         4-11                c
FEATURES             Location/Qualifiers
     source          1..986
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 74.67 cM"
     gene            1..986
                     /gene="Cst7"
                     /gene_synonym="Cmap"
                     /note="cystatin F (leukocystatin)"
                     /db_xref="GeneID:13011"
                     /db_xref="MGI:MGI:1298217"
     exon            1..171
                     /gene="Cst7"
                     /gene_synonym="Cmap"
                     /inference="alignment:Splign:2.1.0"
     CDS             105..539
                     /gene="Cst7"
                     /gene_synonym="Cmap"
                     /note="cystatin-F; cystatin-7; cystatin-like
                     metastasis-associated protein"
                     /codon_start=1
                     /product="cystatin-F precursor"
                     /protein_id="NP_034107.2"
                     /db_xref="CCDS:CCDS16857.2"
                     /db_xref="GeneID:13011"
                     /db_xref="MGI:MGI:1298217"
                     /translation="MWLAILLALCCLTSDTHGARPPDFCSKDLISSVKPGFPKTIETN
                     NPGVLKAARHSVEKFNNCTNDIFLFKESHVSKALVQVVKGLKYMLEVKIGRTTCRKTM
                     HHQLDNCDFQTNPALKRTLYCYSEVWVIPWLHSFEVPVLLCQ"
     exon            172..344
                     /gene="Cst7"
                     /gene_synonym="Cmap"
                     /inference="alignment:Splign:2.1.0"
     exon            345..461
                     /gene="Cst7"
                     /gene_synonym="Cmap"
                     /inference="alignment:Splign:2.1.0"
     exon            462..986
                     /gene="Cst7"
                     /gene_synonym="Cmap"
                     /inference="alignment:Splign:2.1.0"
     regulatory      960..965
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Cst7"
                     /gene_synonym="Cmap"
                     /note="hexamer: AATAAA"
     polyA_site      982
                     /gene="Cst7"
                     /gene_synonym="Cmap"
                     /note="major polyA site"
ORIGIN      
        1 agcaagaaac cacgccccac tgaagctacc ccaccatgcc ctggtcctgg agctgtactt
       61 gccgagcact tggcccacta catgctccct gcatttcccc agccatgtgg ctggccattc
      121 tgcttgccct ctgctgccta acttctgaca cccatggggc acgcccccca gatttttgtt
      181 ccaaagattt gatctccagt gtgaagccag gattccccaa aacaatagag accaataacc
      241 caggagtgct taaggccgcc aggcacagtg tggaaaagtt caacaactgc acaaatgaca
      301 tctttttgtt caaggagtcc catgtcagca aagccctggt acaggtggtg aaaggcctga
      361 aatatatgct ggaggtgaaa atcggccgaa ctacatgcag gaagaccatg catcaccaac
      421 tggacaactg tgacttccaa accaaccctg ccttgaagcg gactctatat tgctactctg
      481 aagtctgggt catcccctgg ctccacagtt ttgaggtgcc tgttctcctc tgccagtgac
      541 ttctgtctct ttagcaagac tgcagctgtg atacatatcc actcgtgttt tccccactcc
      601 ttactttcca ccccatcctg gccatctcag atcctcaggg cctgcttacc agcgagcaga
      661 ttaacatgcc agtgggtcat cagggggctg gactgggaga atatagtgcc ctcctctcca
      721 gattaactgt attcacactt tcttctaaaa tgaattctct ggtgctacac acctagattc
      781 aggatgtggg cacgcagaga gtcctttgat gacattactg gagcacgtgc agagcaaagc
      841 agatacagtc aacaaggtgg tagtgtggga agcttcttga tgcccgactg catgagcaga
      901 ttctgcagcc gtctggtctg ctaccatcag actaatattg atggggatga tttatcataa
      961 ataaaaagct gatcacctaa aagcaa
//
