LOCUS       NM_009976                813 bp    mRNA    linear   ROD 24-SEP-2024
DEFINITION  Mus musculus cystatin C (Cst3), mRNA.
ACCESSION   NM_009976
VERSION     NM_009976.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 813)
  AUTHORS   Yang,H., Han,R.Y., Gong,R.W., Zhang,Y.J., Yang,S.S., Xu,G.Z. and
            Liu,W.
  TITLE     CST3 alleviates retinal vascular leakage by regulating the Rap1
            signaling pathway
  JOURNAL   Exp Eye Res 247, 110042 (2024)
   PUBMED   39147193
  REMARK    GeneRIF: CST3 alleviates retinal vascular leakage by regulating the
            Rap1 signaling pathway.
REFERENCE   2  (bases 1 to 813)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 813)
  AUTHORS   Liu,L., Jiang,Y. and Steinle,J.J.
  TITLE     Loss of cystatin C regulates permeability and inflammatory pathways
            in retina
  JOURNAL   Microvasc Res 148, 104510 (2023)
   PUBMED   36822364
  REMARK    GeneRIF: Loss of cystatin C regulates permeability and inflammatory
            pathways in retina.
REFERENCE   4  (bases 1 to 813)
  AUTHORS   Zhang,R., Hausladen,A., Qian,Z., Liao,X., Premont,R.T. and
            Stamler,J.S.
  TITLE     Hypoxic vasodilatory defect and pulmonary hypertension in mice
            lacking hemoglobin beta-cysteine93 S-nitrosylation
  JOURNAL   JCI Insight 7 (3), e155234 (2022)
   PUBMED   34914637
  REMARK    GeneRIF: Hypoxic vasodilatory defect and pulmonary hypertension in
            mice lacking hemoglobin beta-cysteine93 S-nitrosylation.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 813)
  AUTHORS   Torregrosa-Carrion,R., Pineiro-Sabaris,R., Siguero-Alvarez,M.,
            Grego-Bessa,J., Luna-Zurita,L., Fernandes,V.S., MacGrogan,D.,
            Stainier,D.Y.R. and de la Pompa,J.L.
  TITLE     Adhesion G protein-coupled receptor Gpr126/Adgrg6 is essential for
            placental development
  JOURNAL   Sci Adv 7 (46), eabj5445 (2021)
   PUBMED   34767447
REFERENCE   6  (bases 1 to 813)
  AUTHORS   Brinkmeier,M.L. and Camper,S.A.
  TITLE     Localization of somatostatin receptor genes on mouse chromosomes 2,
            11, 12, 15, and 17: correlation with growth QTLs
  JOURNAL   Genomics 43 (1), 9-14 (1997)
   PUBMED   9226367
REFERENCE   7  (bases 1 to 813)
  AUTHORS   Hakansson,K., Huh,C., Grubb,A., Karlsson,S. and Abrahamson,M.
  TITLE     Mouse and rat cystatin C: Escherichia coli production,
            characterization and tissue distribution
  JOURNAL   Comp Biochem Physiol B Biochem Mol Biol 114 (3), 303-311 (1996)
   PUBMED   8761177
REFERENCE   8  (bases 1 to 813)
  AUTHORS   Huh,C., Nagle,J.W., Kozak,C.A., Abrahamson,M. and Karlsson,S.
  TITLE     Structural organization, expression and chromosomal mapping of the
            mouse cystatin-C-encoding gene (Cst3)
  JOURNAL   Gene 152 (2), 221-226 (1995)
   PUBMED   7835704
REFERENCE   9  (bases 1 to 813)
  AUTHORS   Tu,G.F., Aldred,A.R., Southwell,B.R. and Schreiber,G.
  TITLE     Strong conservation of the expression of cystatin C gene in choroid
            plexus
  JOURNAL   Am J Physiol 263 (1 Pt 2), R195-R200 (1992)
   PUBMED   1636787
REFERENCE   10 (bases 1 to 813)
  AUTHORS   Solem,M., Rawson,C., Lindburg,K. and Barnes,D.
  TITLE     Transforming growth factor beta regulates cystatin C in serum-free
            mouse embryo (SFME) cells
  JOURNAL   Biochem Biophys Res Commun 172 (2), 945-951 (1990)
   PUBMED   2241983
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from CK621009.1, AK002438.1 and
            AI835804.1.
            
            On Aug 20, 2015 this sequence version replaced NM_009976.3.
            
            Summary: The protein encoded by this gene is a cysteine protease
            inhibitor involved in neurodegenerative and cardiovascular
            processes. The encoded protein inhibits aggregation of beta-amyloid
            protein, a hallmark of Alzheimer's disease, so it may be useful as
            a therapeutic. This protein also may be a biomarker for
            atherosclerosis. [provided by RefSeq, Aug 2015].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: CA457140.1, CB205647.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849380
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-44                CK621009.1         65-108
            45-786              AK002438.1         2-743
            787-813             AI835804.1         1-27                c
FEATURES             Location/Qualifiers
     source          1..813
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 73.6 cM"
     gene            1..813
                     /gene="Cst3"
                     /gene_synonym="CysC"
                     /note="cystatin C"
                     /db_xref="GeneID:13010"
                     /db_xref="MGI:MGI:102519"
     exon            1..360
                     /gene="Cst3"
                     /gene_synonym="CysC"
                     /inference="alignment:Splign:2.1.0"
     CDS             136..558
                     /gene="Cst3"
                     /gene_synonym="CysC"
                     /note="cystatin-3"
                     /codon_start=1
                     /product="cystatin-C precursor"
                     /protein_id="NP_034106.2"
                     /db_xref="CCDS:CCDS16852.1"
                     /db_xref="GeneID:13010"
                     /db_xref="MGI:MGI:102519"
                     /translation="MASPLRSLLFLLAVLAVAWAATPKQGPRMLGAPEEADANEEGVR
                     RALDFAVSEYNKGSNDAYHSRAIQVVRARKQLVAGVNYFLDVEMGRTTCTKSQTNLTD
                     CPFHDQPHLMRKALCSFQIYSVPWKGTHSLTKFSCKNA"
     exon            361..474
                     /gene="Cst3"
                     /gene_synonym="CysC"
                     /inference="alignment:Splign:2.1.0"
     exon            475..796
                     /gene="Cst3"
                     /gene_synonym="CysC"
                     /inference="alignment:Splign:2.1.0"
     regulatory      772..777
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Cst3"
                     /gene_synonym="CysC"
                     /note="hexamer: ATTAAA"
     polyA_site      796
                     /gene="Cst3"
                     /gene_synonym="CysC"
                     /note="major polyA site"
ORIGIN      
        1 cggaaaagga ggagccagga gaggcgggcg ggtccctaac gctcgcattt gggtaaaagt
       61 cgcacggagt agcagcgtct gttctgcacc aactcagagt cttgttggag ctttatccct
      121 ttgtcctagc caaccatggc cagcccgctg cgctccttgc tgttcctgct ggccgtcctg
      181 gccgtggcct gggcggcgac cccaaaacaa ggcccgcgaa tgttgggagc cccggaggag
      241 gcagatgcca atgaggaagg cgtgcggcga gcgttggact tcgctgtgag cgagtacaac
      301 aagggcagca acgatgcgta ccacagccgc gccatacagg tggtgagagc tcgtaagcag
      361 ctcgtggctg gagtgaacta ttttttggat gtggagatgg gccgaactac atgtaccaag
      421 tcccagacaa atttgactga ctgtcctttc catgaccagc cccatctgat gaggaaggca
      481 ctctgctcct tccagatcta cagcgtgccc tggaaaggca cacactccct gacaaaattc
      541 agctgcaaaa atgcctaagg gctgagtcta gaaggatcat gcagactgtt ccttacttgt
      601 gctccttccc tatagtgttt catctcgcag aagggtgctc cggctctgga gggcaccgcc
      661 agtgtgtttg caccaggaga cagtaaagga gctgctgcag gcaggttctg cacatctgaa
      721 cagctgtccc ctggctccac tcttcttgca gtacctgcca tgccttgctc aattaaaaaa
      781 aaaaaactcc tttggcaaaa aaaaaaaaaa aaa
//
