LOCUS       NM_001418705             964 bp    mRNA    linear   ROD 02-JUN-2024
DEFINITION  Mus musculus cystatin 13 (Cst13), transcript variant 2, mRNA.
ACCESSION   NM_001418705 XM_011239766
VERSION     NM_001418705.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 964)
  AUTHORS   Frygelius,J., Arvestad,L., Wedell,A. and Tohonen,V.
  TITLE     Evolution and human tissue expression of the Cres/Testatin subgroup
            genes, a reproductive tissue specific subgroup of the type 2
            cystatins
  JOURNAL   Evol Dev 12 (3), 329-342 (2010)
   PUBMED   20565543
REFERENCE   2  (bases 1 to 964)
  AUTHORS   Frygelius,J., Oscarson,M., Nordqvist,K., Wedell,A. and Tohonen,V.
  TITLE     The reproductive tissue specific cystatin subgroup of genes:
            expression during gonadal development in wildtype and testatin
            knockout animals
  JOURNAL   Sex Dev 1 (6), 363-372 (2007)
   PUBMED   18391548
REFERENCE   3  (bases 1 to 964)
  AUTHORS   Xiang,Y., Nie,D.S., Wang,J., Tan,X.J., Deng,Y., Luo,S.W. and
            Lu,G.X.
  TITLE     Cloning, characterization and primary function study of a novel
            gene, Cymg1, related to family 2 cystatins
  JOURNAL   Acta Biochim Biophys Sin (Shanghai) 37 (1), 11-18 (2005)
   PUBMED   15645076
  REMARK    GeneRIF: Cymg1 might play an important role in mouse
            spermatogenesis and sexual maturation
REFERENCE   4  (bases 1 to 964)
  AUTHORS   Xiang,Y., Nie,D.S. and Lu,G.X.
  TITLE     Cloning of a novel gene, Cymg1, related to family 2 cystatins and
            expressed at specific stages of mouse testis development
  JOURNAL   J Genet 83 (3), 257-263 (2004)
   PUBMED   15689628
  REMARK    GeneRIF: Cymg1 expression level varied in the different
            developmental stages of mouse testis, and were coincidental with
            spermatogenesis and sex maturation.
REFERENCE   5  (bases 1 to 964)
  AUTHORS   Cornwall,G.A. and Hsia,N.
  TITLE     A new subgroup of the family 2 cystatins
  JOURNAL   Mol Cell Endocrinol 200 (1-2), 1-8 (2003)
   PUBMED   12644294
  REMARK    Review article
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL844519.9.
            
            On Mar 22, 2023 this sequence version replaced XM_011239766.2.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR5189685.263755.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849384 [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-128               AL844519.9         93974-94101
            129-335             AL844519.9         96375-96581
            336-690             AL844519.9         96829-97183
            691-804             AL844519.9         102009-102122
            805-964             AL844519.9         104118-104277
FEATURES             Location/Qualifiers
     source          1..964
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 73.57 cM"
     gene            1..964
                     /gene="Cst13"
                     /gene_synonym="1700006C19Rik; Cymg1; Mtcg1"
                     /note="cystatin 13"
                     /db_xref="GeneID:69294"
                     /db_xref="MGI:MGI:1916544"
     exon            1..128
                     /gene="Cst13"
                     /gene_synonym="1700006C19Rik; Cymg1; Mtcg1"
                     /inference="alignment:Splign:2.1.0"
     exon            129..335
                     /gene="Cst13"
                     /gene_synonym="1700006C19Rik; Cymg1; Mtcg1"
                     /inference="alignment:Splign:2.1.0"
     exon            336..690
                     /gene="Cst13"
                     /gene_synonym="1700006C19Rik; Cymg1; Mtcg1"
                     /inference="alignment:Splign:2.1.0"
     CDS             463..888
                     /gene="Cst13"
                     /gene_synonym="1700006C19Rik; Cymg1; Mtcg1"
                     /note="cystatin-T"
                     /codon_start=1
                     /product="cystatin-13 precursor"
                     /protein_id="NP_001405634.1"
                     /db_xref="GeneID:69294"
                     /db_xref="MGI:MGI:1916544"
                     /translation="MARFLQTLLFLVIMVEFVSRRVEAWGSPQIVRPFEDIPKSYVYV
                     QHALWYAMKEYNKASNDLYNFRVVDILKSQEQITDSLEYYLEVNIARTMCKKIAGDNE
                     NCLFQQDPKMKKMVFCIFIVSSKPWKFELKMLKKQCKDI"
     exon            691..804
                     /gene="Cst13"
                     /gene_synonym="1700006C19Rik; Cymg1; Mtcg1"
                     /inference="alignment:Splign:2.1.0"
     exon            805..964
                     /gene="Cst13"
                     /gene_synonym="1700006C19Rik; Cymg1; Mtcg1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      933..938
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Cst13"
                     /gene_synonym="1700006C19Rik; Cymg1; Mtcg1"
                     /note="hexamer: AATAAA"
     polyA_site      964
                     /gene="Cst13"
                     /gene_synonym="1700006C19Rik; Cymg1; Mtcg1"
                     /note="major polyA site"
ORIGIN      
        1 agaagcacct ggggctcaga tccccacaga tgcctatctg gaagcaggag catgaacttg
       61 gtatgctggt gatcaagagc aacctgaggc tagaggccat ttaatcttag tcttcactac
      121 agataaagtg tggacaattg ttaaggaaca gatgagcagc ttgatgaaga gttaggcacc
      181 gcaagaaagg atggtacttg gggttttatt catctacatt cctgccccat gtcttcacca
      241 atctggaagc tctgctaatg ctgtgagacg ggcacaattg acaaaatcat tctccactgc
      301 tgatcaactc agacttcatc cccttgaggt caaagccgtg tcaggtgttg aagagaaagg
      361 atccattgac ctgcagagaa agcaggggcc tcactggcta cattgacacc agctctggaa
      421 agaaaatagg aacttggtat gttccttgaa tgaagaagca ccatggccag attcttacag
      481 accctgctgt tcctggtgat catggtggag tttgtatcta gaagagtcga agcctggggc
      541 tccccacaga ttgtgaggcc attcgaagac atccccaaat cctatgtcta tgtgcagcat
      601 gcactctggt atgccatgaa agaatataac aaggccagca atgacctcta caacttcagg
      661 gtggtggata tcctaaaatc tcaggagcag atcacagaca gtctggagta ttatcttgaa
      721 gtaaacattg cccgaacaat gtgcaagaag attgcaggag ataatgaaaa ctgcttgttt
      781 caacaggatc ctaaaatgaa aaagatggtg ttttgcattt ttattgttag ctccaaacca
      841 tggaagtttg aacttaaaat gctgaagaag caatgcaaag atatctaatc agcattcggg
      901 acaccttgcc actcttactt ttcttttaac tgaataaaga tgcttgtaag atagtggacc
      961 acca
//
