LOCUS       NM_027054                537 bp    mRNA    linear   ROD 01-JUN-2024
DEFINITION  Mus musculus cystatin 12 (Cst12), mRNA.
ACCESSION   NM_027054 XM_130487 XM_918155
VERSION     NM_027054.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 537)
  AUTHORS   Do,H.Q., Hewetson,A., Borcik,C.G., Hastert,M.C., Whelly,S.,
            Wylie,B.J., Sutton,R.B. and Cornwall,G.A.
  TITLE     Cross-seeding between the functional amyloidogenic CRES and CRES3
            family members and their regulation of Abeta assembly
  JOURNAL   J Biol Chem 296, 100250 (2021)
   PUBMED   33384380
  REMARK    GeneRIF: Cross-seeding between the functional amyloidogenic CRES
            and CRES3 family members and their regulation of Abeta assembly.
REFERENCE   2  (bases 1 to 537)
  AUTHORS   Frygelius,J., Oscarson,M., Nordqvist,K., Wedell,A. and Tohonen,V.
  TITLE     The reproductive tissue specific cystatin subgroup of genes:
            expression during gonadal development in wildtype and testatin
            knockout animals
  JOURNAL   Sex Dev 1 (6), 363-372 (2007)
   PUBMED   18391548
REFERENCE   3  (bases 1 to 537)
  AUTHORS   Li,Y., Putnam-Lawson,C.A., Knapp-Hoch,H., Friel,P.J., Mitchell,D.,
            Hively,R. and Griswold,M.D.
  TITLE     Immunolocalization and regulation of cystatin 12 in mouse testis
            and epididymis
  JOURNAL   Biol Reprod 73 (5), 872-880 (2005)
   PUBMED   15972886
  REMARK    GeneRIF: CST12 protein may play a specialized role during
            spermatogenesis and sperm maturation
REFERENCE   4  (bases 1 to 537)
  AUTHORS   Hsia,N. and Cornwall,G.A.
  TITLE     Cres2 and Cres3: new members of the cystatin-related epididymal
            spermatogenic subgroup of family 2 cystatins
  JOURNAL   Endocrinology 144 (3), 909-915 (2003)
   PUBMED   12586767
  REMARK    GeneRIF: CRES2 and CRES3 represent new members of a subgroup of
            cystatin family 2 proteins that likely carry out tissue-specific
            functions distinct from that of typical cystatins CRES2, CRES3]
REFERENCE   5  (bases 1 to 537)
  AUTHORS   Cornwall,G.A. and Hsia,N.
  TITLE     A new subgroup of the family 2 cystatins
  JOURNAL   Mol Cell Endocrinol 200 (1-2), 1-8 (2003)
   PUBMED   12644294
  REMARK    Review article
REFERENCE   6  (bases 1 to 537)
  AUTHORS   Li,Y., Friel,P.J., Robinson,M.O., McLean,D.J. and Griswold,M.D.
  TITLE     Identification and characterization of testis- and
            epididymis-specific genes: cystatin SC and cystatin TE-1
  JOURNAL   Biol Reprod 67 (6), 1872-1880 (2002)
   PUBMED   12444065
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BC119432.2, AK005670.1 and AF440737.2.
            
            On Feb 15, 2019 this sequence version replaced NM_027054.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF440737.2, CA463760.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849381
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-473               BC119432.2         5-477
            474-531             AK005670.1         469-526
            532-537             AF440737.2         518-523
FEATURES             Location/Qualifiers
     source          1..537
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 73.55 cM"
     gene            1..537
                     /gene="Cst12"
                     /gene_synonym="1700006F03Rik; CRES3"
                     /note="cystatin 12"
                     /db_xref="GeneID:69362"
                     /db_xref="MGI:MGI:1916612"
     exon            1..230
                     /gene="Cst12"
                     /gene_synonym="1700006F03Rik; CRES3"
                     /inference="alignment:Splign:2.1.0"
     CDS             36..422
                     /gene="Cst12"
                     /gene_synonym="1700006F03Rik; CRES3"
                     /note="cystatin-related epididymal spermatogenic protein
                     3; cystatin TE-1; cystatin-related gene expressed in
                     testis and epididymis"
                     /codon_start=1
                     /product="cystatin-12 precursor"
                     /protein_id="NP_081330.2"
                     /db_xref="CCDS:CCDS50737.2"
                     /db_xref="GeneID:69362"
                     /db_xref="MGI:MGI:1916612"
                     /translation="MLWKSVLSVALIVLGIHDCSFKFLEIDKNEEEFAISVEHVVFHF
                     NENQDDDFAYKFLRVRRSLRQKYTLKYLVDLEMGRTLCGKYDEDIDNCPLQEGPGERK
                     VRCTYIVETEAWVTKFTILNSTCVQT"
     exon            231..341
                     /gene="Cst12"
                     /gene_synonym="1700006F03Rik; CRES3"
                     /inference="alignment:Splign:2.1.0"
     exon            342..537
                     /gene="Cst12"
                     /gene_synonym="1700006F03Rik; CRES3"
                     /inference="alignment:Splign:2.1.0"
     regulatory      516..521
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Cst12"
                     /gene_synonym="1700006F03Rik; CRES3"
                     /note="hexamer: ATTAAA"
     polyA_site      537
                     /gene="Cst12"
                     /gene_synonym="1700006F03Rik; CRES3"
                     /note="major polyA site"
ORIGIN      
        1 actctcagct ctcagtgcca actctgaaga cagccatgtt gtggaagtcg gtcctgtccg
       61 tggcactcat tgtgctggga attcatgatt gcagcttcaa gtttctagag atcgacaaga
      121 atgaagagga gtttgccatt tctgtggagc atgtcgtgtt ccacttcaac gaaaaccagg
      181 atgacgattt tgcctacaag ttcctgagag tccggagaag tcttcgtcag aaatatacgc
      241 tgaaatattt agtagacctg gagatgggcc gtacgctttg cgggaaatat gatgaagaca
      301 ttgacaattg tccattgcag gaaggtccag gagagagaaa ggtacgctgc acttacattg
      361 tggagactga agcatgggtt actaagttca ccatcttgaa cagcacctgt gtgcagactt
      421 aagtggggca gaggtcattc tgtgcctata gctaaaggcc cctttcctct agagggtgac
      481 tgcctgtagt ctggggtctc tttagtacca ggacaattaa agtcaactta caaaaca
//
