LOCUS       NM_001286022            1102 bp    mRNA    linear   ROD 05-JUN-2024
DEFINITION  Mus musculus casein beta (Csn2), transcript variant 4, mRNA.
ACCESSION   NM_001286022
VERSION     NM_001286022.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1102)
  AUTHORS   Lin,H., Yan,Y., Luo,Y., So,W.Y., Wei,X., Zhang,X., Yang,X.,
            Zhang,J., Su,Y., Yang,X., Zhang,B., Zhang,K., Jiang,N.,
            Chow,B.K.C., Han,W., Wang,F. and Rao,F.
  TITLE     IP6-assisted CSN-COP1 competition regulates a CRL4-ETV5 proteolytic
            checkpoint to safeguard glucose-induced insulin secretion
  JOURNAL   Nat Commun 12 (1), 2461 (2021)
   PUBMED   33911083
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1102)
  AUTHORS   Kobayashi,K., Oyama,S., Kuki,C., Tsugami,Y., Matsunaga,K.,
            Suzuki,T. and Nishimura,T.
  TITLE     Distinct roles of prolactin, epidermal growth factor, and
            glucocorticoids in beta-casein secretion pathway in lactating
            mammary epithelial cells
  JOURNAL   Mol Cell Endocrinol 440, 16-24 (2017)
   PUBMED   27836773
  REMARK    GeneRIF: These results indicate that PRL, EGF and glucocorticoids
            have distinct roles in the establishment of beta-casein secretion
            pathway.
REFERENCE   3  (bases 1 to 1102)
  AUTHORS   Kobayashi,K., Tsugami,Y., Matsunaga,K., Oyama,S., Kuki,C. and
            Kumura,H.
  TITLE     Prolactin and glucocorticoid signaling induces lactation-specific
            tight junctions concurrent with beta-casein expression in mammary
            epithelial cells
  JOURNAL   Biochim Biophys Acta 1863 (8), 2006-2016 (2016)
   PUBMED   27130254
  REMARK    GeneRIF: Dexamethasone signaling upregulates Claudin-3 and -4
            without beta-casein expression. Prolactin and dexamethasone
            signaling synergistically upregulate beta-casein expression.
REFERENCE   4  (bases 1 to 1102)
  AUTHORS   Elias,S., Thion,M.S., Yu,H., Sousa,C.M., Lasgi,C., Morin,X. and
            Humbert,S.
  TITLE     Huntingtin regulates mammary stem cell division and differentiation
  JOURNAL   Stem Cell Reports 2 (4), 491-506 (2014)
   PUBMED   24749073
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 1102)
  AUTHORS   Qian,X. and Zhao,F.Q.
  TITLE     Interactions of the ubiquitous octamer-binding transcription
            factor-1 with both the signal transducer and activator of
            transcription 5 and the glucocorticoid receptor mediate prolactin
            and glucocorticoid-induced beta-casein gene expression in mammary
            epithelial cells
  JOURNAL   Int J Biochem Cell Biol 45 (3), 724-735 (2013)
   PUBMED   23313770
  REMARK    GeneRIF: octamer-binding transcription factor-1 may serve as a
            master regulator that facilitates the DNA binding of both signal
            transducer and activator of transcription 5 and glucocorticoid
            receptor in hormone-induced beta-casein expression
REFERENCE   6  (bases 1 to 1102)
  AUTHORS   Schmitt-Ney,M., Happ,B., Ball,R.K. and Groner,B.
  TITLE     Developmental and environmental regulation of a mammary
            gland-specific nuclear factor essential for transcription of the
            gene encoding beta-casein
  JOURNAL   Proc Natl Acad Sci U S A 89 (7), 3130-3134 (1992)
   PUBMED   1557422
REFERENCE   7  (bases 1 to 1102)
  AUTHORS   Lee,C.S. and Oka,T.
  TITLE     A pregnancy-specific mammary nuclear factor involved in the
            repression of the mouse beta-casein gene transcription by
            progesterone
  JOURNAL   J Biol Chem 267 (9), 5797-5801 (1992)
   PUBMED   1556095
REFERENCE   8  (bases 1 to 1102)
  AUTHORS   Schmitt-Ney,M., Doppler,W., Ball,R.K. and Groner,B.
  TITLE     Beta-casein gene promoter activity is regulated by the
            hormone-mediated relief of transcriptional repression and a
            mammary-gland-specific nuclear factor
  JOURNAL   Mol Cell Biol 11 (7), 3745-3755 (1991)
   PUBMED   2046676
REFERENCE   9  (bases 1 to 1102)
  AUTHORS   Yoshimura,M. and Oka,T.
  TITLE     Transfection of beta-casein chimeric gene and hormonal induction of
            its expression in primary murine mammary epithelial cells
  JOURNAL   Proc Natl Acad Sci U S A 87 (10), 3670-3674 (1990)
   PUBMED   2187188
REFERENCE   10 (bases 1 to 1102)
  AUTHORS   Yoshimura,M. and Oka,T.
  TITLE     Isolation and structural analysis of the mouse beta-casein gene
  JOURNAL   Gene 78 (2), 267-275 (1989)
   PUBMED   2673924
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC074046.9 and AK085698.1.
            
            Transcript Variant: This variant (4) lacks an alternate in-frame
            exon in the 5' coding region, and uses an alternate in-frame splice
            site in the 3' coding region, compared to variant 1. The encoded
            isoform (c) is shorter than isoform a.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: partial sample support SAMN00849383 [ECO:0000350]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-4                 AC074046.9         146525-146528
            5-110               AK085698.1         2-107
            111-1093            AK085698.1         132-1114
            1094-1102           AC074046.9         153323-153331
FEATURES             Location/Qualifiers
     source          1..1102
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="5"
                     /map="5 43.56 cM"
     gene            1..1102
                     /gene="Csn2"
                     /gene_synonym="Csnb"
                     /note="casein beta"
                     /db_xref="GeneID:12991"
                     /db_xref="MGI:MGI:88541"
     exon            1..47
                     /gene="Csn2"
                     /gene_synonym="Csnb"
                     /inference="alignment:Splign:2.1.0"
     exon            48..110
                     /gene="Csn2"
                     /gene_synonym="Csnb"
                     /inference="alignment:Splign:2.1.0"
     CDS             60..728
                     /gene="Csn2"
                     /gene_synonym="Csnb"
                     /note="isoform c precursor is encoded by transcript
                     variant 4; beta-casein"
                     /codon_start=1
                     /product="beta-casein isoform c precursor"
                     /protein_id="NP_001272951.1"
                     /db_xref="GeneID:12991"
                     /db_xref="MGI:MGI:88541"
                     /translation="MKVFILACLVALALARETDSISSEESVEHINEKLQKVNLMGQLQ
                     AEDVLQAKVHSSIQSQPQAFPYAQAQTISCNPVPQNIQPIAQPPVVPSLGPVISPELE
                     SFLKAKATILPKHKQMPLLNSETVLRLINSQIPSLASLANLHLPQSLVQLLAQVVQAF
                     PQTHLVSSQTQLSLPQSKVLYFLQQVAPFLPQDMSVQDLLQYLELLNPTVQFPATPQH
                     SVSV"
     exon            111..131
                     /gene="Csn2"
                     /gene_synonym="Csnb"
                     /inference="alignment:Splign:2.1.0"
     exon            132..155
                     /gene="Csn2"
                     /gene_synonym="Csnb"
                     /inference="alignment:Splign:2.1.0"
     exon            156..197
                     /gene="Csn2"
                     /gene_synonym="Csnb"
                     /inference="alignment:Splign:2.1.0"
     exon            198..722
                     /gene="Csn2"
                     /gene_synonym="Csnb"
                     /inference="alignment:Splign:2.1.0"
     exon            723..767
                     /gene="Csn2"
                     /gene_synonym="Csnb"
                     /inference="alignment:Splign:2.1.0"
     exon            768..1102
                     /gene="Csn2"
                     /gene_synonym="Csnb"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1074..1079
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Csn2"
                     /gene_synonym="Csnb"
                     /note="hexamer: AATAAA"
     polyA_site      1094
                     /gene="Csn2"
                     /gene_synonym="Csnb"
                     /note="major polyA site"
ORIGIN      
        1 ggacatcatc ctttcagctt cacctcctct cttgtcctcc actaaaggac ttgacagcca
       61 tgaaggtctt catcctcgcc tgccttgtgg cccttgctct tgcaagagag actgatagta
      121 tttccagtga ggaatctgtt gaacatatca atgagaaact tcagaaggtg aatctcatgg
      181 gacagctgca ggcagaggat gtgctccagg ctaaagttca ctccagcatc cagtcacagc
      241 cccaggcctt tccatatgct caggctcaaa ccatctcttg caatcccgtc ccacaaaaca
      301 tccagcctat tgctcaaccc cctgtggtgc catctcttgg gcctgtcatt tctcctgaac
      361 tggaatcctt ccttaaagct aaagccacca tccttcccaa gcacaaacag atgcccctcc
      421 ttaactctga aactgtgctc cgcctcataa actctcaaat ccccagcctt gccagtcttg
      481 ctaatctgca ccttcctcag tctctggtcc agctcctggc acaggttgtt caggcttttc
      541 ctcagactca cctggtttct tctcagaccc agctgtctct tcctcagtcc aaagttctgt
      601 actttctgca gcaagtagca cccttccttc cacaagatat gtctgtccaa gaccttctgc
      661 agtacctaga acttcttaac cccaccgtcc aattccctgc cactccacaa cattccgttt
      721 ctgtctaaga ggatttccag gttattttct cctccttatg ttttttgaac tgactgaaac
      781 tggaaatgta caaatctttt cacctttgga tcatgctaca aaaatgatat atttctgaat
      841 taatctacat ggaagaaaag aaaatgtatt cttttattta ttctatgtat tatatggcat
      901 tcatttgaat tcgacccatg gactctatat gcttctcagt tatattacag gaattttata
      961 agtgttcaat atggagttga aaatgcaagt caataatgta tacaaaatag tttgtgaaaa
     1021 attggatttt ctattttttt tctttgagaa cctatttcct ttccagtcat ttcaataaaa
     1081 taatccttta ggcatattta ag
//
