LOCUS       NM_009973                766 bp    mRNA    linear   ROD 31-MAY-2024
DEFINITION  Mus musculus casein alpha s2-like B (Csn1s2b), transcript variant
            1, mRNA.
ACCESSION   NM_009973
VERSION     NM_009973.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 766)
  AUTHORS   Lee,H.K., Willi,M., Kuhns,T., Liu,C. and Hennighausen,L.
  TITLE     Redundant and non-redundant cytokine-activated enhancers control
            Csn1s2b expression in the lactating mouse mammary gland
  JOURNAL   Nat Commun 12 (1), 2239 (2021)
   PUBMED   33854063
  REMARK    GeneRIF: Redundant and non-redundant cytokine-activated enhancers
            control Csn1s2b expression in the lactating mouse mammary gland.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 766)
  AUTHORS   Rijnkels,M., Elnitski,L., Miller,W. and Rosen,J.M.
  TITLE     Multispecies comparative analysis of a mammalian-specific genomic
            domain encoding secretory proteins
  JOURNAL   Genomics 82 (4), 417-432 (2003)
   PUBMED   13679022
REFERENCE   3  (bases 1 to 766)
  AUTHORS   George,S., Clark,A.J. and Archibald,A.L.
  TITLE     Physical mapping of the murine casein locus reveals the gene order
            as alpha-beta-gamma-epsilon-kappa
  JOURNAL   DNA Cell Biol 16 (4), 477-484 (1997)
   PUBMED   9150435
REFERENCE   4  (bases 1 to 766)
  AUTHORS   Rijnkels,M., Wheeler,D.A., de Boer,H.A. and Pieper,F.R.
  TITLE     Structure and expression of the mouse casein gene locus
  JOURNAL   Mamm Genome 8 (1), 9-15 (1997)
   PUBMED   9021141
REFERENCE   5  (bases 1 to 766)
  AUTHORS   Tomlinson,A.M., Cox,R.D., Lehrach,H.R. and Dalrymple,M.A.
  TITLE     Restriction map of two yeast artificial chromosomes spanning the
            murine casein locus
  JOURNAL   Mamm Genome 7 (7), 542-544 (1996)
   PUBMED   8672136
REFERENCE   6  (bases 1 to 766)
  AUTHORS   Hennighausen,L.G., Steudle,A. and Sippel,A.E.
  TITLE     Nucleotide sequence of cloned cDNA coding for mouse epsilon casein
  JOURNAL   Eur J Biochem 126 (3), 569-572 (1982)
   PUBMED   6897225
REFERENCE   7  (bases 1 to 766)
  AUTHORS   Hennighausen,L.G. and Sippel,A.E.
  TITLE     Characterization and cloning of the mRNAs specific for the
            lactating mouse mammary gland
  JOURNAL   Eur J Biochem 125 (1), 131-141 (1982)
   PUBMED   6896688
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AK164767.1 and AK085744.1.
            
            On Aug 7, 2014 this sequence version replaced NM_009973.2.
            
            Summary: This gene is a member of the alpha-s2-like casein gene
            family, and this gene product is a calcium-sensitive casein.
            Members of this gene family are organized as a gene cluster that is
            conserved in its order, but with greater conservation amongst
            orthologs than paralogs. The protein encoded by this gene interacts
            with other casein proteins to form a micelle structure, and is a
            major source of protein in milk. This structure is important for
            the transport of calcium, phosphate, and protein. Alternative
            splicing results in multiple transcript variants encoding different
            protein isoforms. [provided by RefSeq, Aug 2014].
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (1).
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK164767.1, AK085744.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849384,
                                           SAMN01164133 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1                 AK164767.1         1-1
            2-766               AK085744.1         2-766
FEATURES             Location/Qualifiers
     source          1..766
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="5"
                     /map="5 43.56 cM"
     gene            1..766
                     /gene="Csn1s2b"
                     /gene_synonym="Csnd; Csne"
                     /note="casein alpha s2-like B"
                     /db_xref="GeneID:12992"
                     /db_xref="MGI:MGI:105312"
     exon            1..45
                     /gene="Csn1s2b"
                     /gene_synonym="Csnd; Csne"
                     /inference="alignment:Splign:2.1.0"
     exon            46..108
                     /gene="Csn1s2b"
                     /gene_synonym="Csnd; Csne"
                     /inference="alignment:Splign:2.1.0"
     CDS             58..489
                     /gene="Csn1s2b"
                     /gene_synonym="Csnd; Csne"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; alpha-S2-casein-like B; epsilon-casein; casein
                     delta"
                     /codon_start=1
                     /product="alpha-S2-casein-like B isoform 1 precursor"
                     /protein_id="NP_034103.2"
                     /db_xref="CCDS:CCDS39133.1"
                     /db_xref="GeneID:12992"
                     /db_xref="MGI:MGI:105312"
                     /translation="MKFIILTCLLAVALAKQRMEQYISSEESMDNSQENFKQNMDVAF
                     FPSQETVENIYIPQMESVEAPMKVSDIISQQQYNQKMMDMSVSAREKTVMTEESKNIQ
                     DYMNKMKRYSKITWPQFVKLLHQYQKTMTPWSYYPSTPSQV"
     exon            109..135
                     /gene="Csn1s2b"
                     /gene_synonym="Csnd; Csne"
                     /inference="alignment:Splign:2.1.0"
     exon            136..159
                     /gene="Csn1s2b"
                     /gene_synonym="Csnd; Csne"
                     /inference="alignment:Splign:2.1.0"
     exon            160..201
                     /gene="Csn1s2b"
                     /gene_synonym="Csnd; Csne"
                     /inference="alignment:Splign:2.1.0"
     exon            202..234
                     /gene="Csn1s2b"
                     /gene_synonym="Csnd; Csne"
                     /inference="alignment:Splign:2.1.0"
     exon            235..258
                     /gene="Csn1s2b"
                     /gene_synonym="Csnd; Csne"
                     /inference="alignment:Splign:2.1.0"
     exon            259..282
                     /gene="Csn1s2b"
                     /gene_synonym="Csnd; Csne"
                     /inference="alignment:Splign:2.1.0"
     exon            283..327
                     /gene="Csn1s2b"
                     /gene_synonym="Csnd; Csne"
                     /inference="alignment:Splign:2.1.0"
     exon            328..372
                     /gene="Csn1s2b"
                     /gene_synonym="Csnd; Csne"
                     /inference="alignment:Splign:2.1.0"
     exon            373..483
                     /gene="Csn1s2b"
                     /gene_synonym="Csnd; Csne"
                     /inference="alignment:Splign:2.1.0"
     exon            484..766
                     /gene="Csn1s2b"
                     /gene_synonym="Csnd; Csne"
                     /inference="alignment:Splign:2.1.0"
     regulatory      746..751
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Csn1s2b"
                     /gene_synonym="Csnd; Csne"
                     /note="hexamer: AATAAA"
     polyA_site      763
                     /gene="Csn1s2b"
                     /gene_synonym="Csnd; Csne"
                     /note="major polyA site"
ORIGIN      
        1 ggtctctcat tgcctggaca gcttgtcttc aacttaggaa gcaaggactc attaatcatg
       61 aagttcatca ttcttacttg ccttttggcc gttgctcttg caaagcagag gatggagcaa
      121 tacatctcca gtgaggagtc catggataac tctcaagaaa actttaagca gaatatggat
      181 gtggcctttt ttcccagtca ggaaactgta gagaacattt acattcccca aatggaatct
      241 gttgaagctc ccatgaaggt atctgacatc atttctcagc aacaatacaa ccagaaaatg
      301 atggacatga gtgtgagtgc cagagagaaa actgtgatga ctgaagaaag taagaacatc
      361 caagactaca tgaacaaaat gaaacgatac agcaagatca cctggccaca gtttgtaaag
      421 cttctccatc aataccagaa aaccatgacc ccgtggagct attacccatc tacccccagc
      481 caggtttgag tggaaaatcc acatctccat tttcttttct gctgtaatta cttctcatta
      541 ccaacatatt gggatagatg tataaatgat acagaagtgg taaaccaaca gaatgctcca
      601 aaatagttcc agagtatctt ctagtcttgt acatttccat tgaatgtgtt aggtctgctt
      661 gtgggtttca tctctgcagg gttgatgact attgagactg tcttcacttc tgctcccatg
      721 tcactgaacc acattctttt caaaaaataa aacttcaagt gcatgc
//
