LOCUS       NM_030165               3676 bp    mRNA    linear   ROD 04-JUN-2024
DEFINITION  Mus musculus chondroitin sulfate N-acetylgalactosaminyltransferase
            2 (Csgalnact2), transcript variant 2, mRNA.
ACCESSION   NM_030165
VERSION     NM_030165.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3676)
  AUTHORS   Adhikara,I.M., Yagi,K., Mayasari,D.S., Suzuki,Y., Ikeda,K.,
            Ryanto,G.R.T., Sasaki,N., Rikitake,Y., Nadanaka,S., Kitagawa,H.,
            Miyata,O., Igarashi,M., Hirata,K.I. and Emoto,N.
  TITLE     Chondroitin Sulfate N-acetylgalactosaminyltransferase-2 Impacts
            Foam Cell Formation and Atherosclerosis by Altering Macrophage
            Glycosaminoglycan Chain
  JOURNAL   Arterioscler Thromb Vasc Biol 41 (3), 1076-1091 (2021)
   PUBMED   33504177
  REMARK    GeneRIF: Chondroitin Sulfate N-acetylgalactosaminyltransferase-2
            Impacts Foam Cell Formation and Atherosclerosis by Altering
            Macrophage Glycosaminoglycan Chain.
REFERENCE   2  (bases 1 to 3676)
  AUTHORS   Bachvarova,V., Dierker,T., Esko,J., Hoffmann,D., Kjellen,L. and
            Vortkamp,A.
  TITLE     Chondrocytes respond to an altered heparan sulfate composition with
            distinct changes of heparan sulfate structure and increased levels
            of chondroitin sulfate
  JOURNAL   Matrix Biol 93, 43-59 (2020)
   PUBMED   32201365
REFERENCE   3  (bases 1 to 3676)
  AUTHORS   Adhikara,I.M., Yagi,K., Mayasari,D.S., Ikeda,K., Kitagawa,H.,
            Miyata,O., Igarashi,M., Hatakeyama,K., Asada,Y., Hirata,K.I. and
            Emoto,N.
  TITLE     Chondroitin sulfate N-acetylgalactosaminyltransferase-2 deletion
            alleviates lipoprotein retention in early atherosclerosis and
            attenuates aortic smooth muscle cell migration
  JOURNAL   Biochem Biophys Res Commun 509 (1), 89-95 (2019)
   PUBMED   30579596
  REMARK    GeneRIF: Chondroitin sulfate N-acetylgalactosaminyltransferase 2 is
            functionally involved in the progression of atherosclerosis both in
            its early and advanced stages.
REFERENCE   4  (bases 1 to 3676)
  AUTHORS   Uijtdewilligen,P.J.E., Versteeg,E.M., van de Westerlo,E.M.A., van
            der Vlag,J., Daamen,W.F. and van Kuppevelt,T.H.
  TITLE     Dynamic Expression of Genes Involved in
            Proteoglycan/Glycosaminoglycan Metabolism during Skin Development
  JOURNAL   Biomed Res Int 2018, 9873471 (2018)
   PUBMED   30228991
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 3676)
  AUTHORS   Shimbo,M., Suzuki,R., Fuseya,S., Sato,T., Kiyohara,K., Hagiwara,K.,
            Okada,R., Wakui,H., Tsunakawa,Y., Watanabe,H., Kimata,K.,
            Narimatsu,H., Kudo,T. and Takahashi,S.
  TITLE     Postnatal lethality and chondrodysplasia in mice lacking both
            chondroitin sulfate N-acetylgalactosaminyltransferase-1 and -2
  JOURNAL   PLoS One 12 (12), e0190333 (2017)
   PUBMED   29287114
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 3676)
  AUTHORS   Izumikawa,T., Okuura,Y., Koike,T., Sakoda,N. and Kitagawa,H.
  TITLE     Chondroitin 4-O-sulfotransferase-1 regulates the chain length of
            chondroitin sulfate in co-operation with chondroitin
            N-acetylgalactosaminyltransferase-2
  JOURNAL   Biochem J 434 (2), 321-331 (2011)
   PUBMED   21138417
REFERENCE   7  (bases 1 to 3676)
  AUTHORS   Sato,T., Kudo,T., Ikehara,Y., Ogawa,H., Hirano,T., Kiyohara,K.,
            Hagiwara,K., Togayachi,A., Ema,M., Takahashi,S., Kimata,K.,
            Watanabe,H. and Narimatsu,H.
  TITLE     Chondroitin sulfate N-acetylgalactosaminyltransferase 1 is
            necessary for normal endochondral ossification and aggrecan
            metabolism
  JOURNAL   J Biol Chem 286 (7), 5803-5812 (2011)
   PUBMED   21148564
REFERENCE   8  (bases 1 to 3676)
  AUTHORS   Watanabe,Y., Takeuchi,K., Higa Onaga,S., Sato,M., Tsujita,M.,
            Abe,M., Natsume,R., Li,M., Furuichi,T., Saeki,M., Izumikawa,T.,
            Hasegawa,A., Yokoyama,M., Ikegawa,S., Sakimura,K., Amizuka,N.,
            Kitagawa,H. and Igarashi,M.
  TITLE     Chondroitin sulfate N-acetylgalactosaminyltransferase-1 is required
            for normal cartilage development
  JOURNAL   Biochem J 432 (1), 47-55 (2010)
   PUBMED   20812917
REFERENCE   9  (bases 1 to 3676)
  AUTHORS   Sakai,K., Kimata,K., Sato,T., Gotoh,M., Narimatsu,H., Shinomiya,K.
            and Watanabe,H.
  TITLE     Chondroitin sulfate N-acetylgalactosaminyltransferase-1 plays a
            critical role in chondroitin sulfate synthesis in cartilage
  JOURNAL   J Biol Chem 282 (6), 4152-4161 (2007)
   PUBMED   17145758
REFERENCE   10 (bases 1 to 3676)
  AUTHORS   Silbert,J.E. and Freilich,L.S.
  TITLE     Biosynthesis of chondroitin sulphate by a Golgi-apparatus-enriched
            preparation from cultures of mouse mastocytoma cells
  JOURNAL   Biochem J 190 (2), 307-313 (1980)
   PUBMED   6781479
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK019496.1, AK028045.1 and BC060169.1.
            
            On Jan 28, 2006 this sequence version replaced NM_030165.2.
            
            Transcript Variant: This variant (2) differs in the 5' UTR compared
            to variant 1. Variants 1 and 2 both encode the same protein.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK145992.1, AK028045.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164133, SAMN01164134
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-31                AK019496.1         4-34
            32-2486             AK028045.1         13-2467
            2487-3676           BC060169.1         4090-5279
FEATURES             Location/Qualifiers
     source          1..3676
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="6"
                     /map="6 55.86 cM"
     gene            1..3676
                     /gene="Csgalnact2"
                     /gene_synonym="4632415D10Rik; Galnact2"
                     /note="chondroitin sulfate
                     N-acetylgalactosaminyltransferase 2"
                     /db_xref="GeneID:78752"
                     /db_xref="MGI:MGI:1926002"
     exon            1..118
                     /gene="Csgalnact2"
                     /gene_synonym="4632415D10Rik; Galnact2"
                     /inference="alignment:Splign:2.1.0"
     exon            119..1043
                     /gene="Csgalnact2"
                     /gene_synonym="4632415D10Rik; Galnact2"
                     /inference="alignment:Splign:2.1.0"
     CDS             383..2011
                     /gene="Csgalnact2"
                     /gene_synonym="4632415D10Rik; Galnact2"
                     /EC_number="2.4.1.174"
                     /note="chondroitin sulfate GalNAcT-2; galNAcT-2;
                     beta4GalNAcT-2; chondroitin
                     beta-1,4-N-acetylgalactosaminyltransferase 2"
                     /codon_start=1
                     /product="chondroitin sulfate
                     N-acetylgalactosaminyltransferase 2"
                     /protein_id="NP_084441.3"
                     /db_xref="CCDS:CCDS20469.1"
                     /db_xref="GeneID:78752"
                     /db_xref="MGI:MGI:1926002"
                     /translation="MSRRGSILHSRTQWLLLGLALLFSLVLFMYLLECAPQTDGNASL
                     PGVVRENYGKEYYQALLQEQEEHYQTRATSLKRQIAQLKQELQDMSEKMRALQERKKL
                     GANGVGYPGNREQAPSDLLEFLHSQIDRAEVSVGAKLPSEYGVVPFESFTLMKVFQLE
                     MGLTRHPEEKPVRKDKRDELVEVIEAGVEVINNPDEDDAQEDEEGPLGEKLIFNENDF
                     IEGYYRTERDKGTQYELFFKKADLMEYRHVTLFRPFGPLMKVKNELIDITRSVINIIV
                     PLAERTEAFSQFMQNFRDVCIHQDKRIHLTVVYFGKEGLSKVKSILESVSSESDFHNY
                     TLVSLDEEFNRGRGLNVGARAWDKGEVLMFFCDVDIYFSAEFLNSCRLNAEPGKKVFY
                     PVVFSLYNPAIVYANQDVPPPVEQQLVHKKDSGFWRDFGFGMTCQYQSDFLSVGGFDM
                     EVKGWGGEDVHLYRKYLHGDLIVIRTPVPGLFHLWHEKHCADELTPEQYRMCIQSKAM
                     NEASHSHLGMMVFREEIEMHLRKQAYRTNSETAG"
     exon            1044..1260
                     /gene="Csgalnact2"
                     /gene_synonym="4632415D10Rik; Galnact2"
                     /inference="alignment:Splign:2.1.0"
     exon            1261..1362
                     /gene="Csgalnact2"
                     /gene_synonym="4632415D10Rik; Galnact2"
                     /inference="alignment:Splign:2.1.0"
     exon            1363..1541
                     /gene="Csgalnact2"
                     /gene_synonym="4632415D10Rik; Galnact2"
                     /inference="alignment:Splign:2.1.0"
     exon            1542..1636
                     /gene="Csgalnact2"
                     /gene_synonym="4632415D10Rik; Galnact2"
                     /inference="alignment:Splign:2.1.0"
     exon            1637..1718
                     /gene="Csgalnact2"
                     /gene_synonym="4632415D10Rik; Galnact2"
                     /inference="alignment:Splign:2.1.0"
     exon            1719..3661
                     /gene="Csgalnact2"
                     /gene_synonym="4632415D10Rik; Galnact2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3633..3638
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Csgalnact2"
                     /gene_synonym="4632415D10Rik; Galnact2"
                     /note="hexamer: AATAAA"
     polyA_site      3661
                     /gene="Csgalnact2"
                     /gene_synonym="4632415D10Rik; Galnact2"
                     /note="major polyA site"
ORIGIN      
        1 tttcctcgcg cgcgccggaa gtggcggtgc tccgcggcgg cgcgccgggc cggccccgcg
       61 cagggcgctg gccaggtgtg caggtcccca gaggcggcga cgctgagcgg cgggcgcgaa
      121 aagtcaccac ccagataatg gattttatag atcagattgc ttagtgtgga tatcatggta
      181 acaatacagg aagtattgtt aatttctcac ttctaccaag tagtcatgac tactgaagaa
      241 agaatctttt aaaactacaa cagagtgcac ttgggtacct cttatggaaa ttctaatttt
      301 gtttttaact tttggtaatt ttttactaaa gtcatgaaca agcagagggc ttgtcctgtc
      361 aggcaaacac actctagtga gaatgtctag aagaggatcg attctgcaca gccggaccca
      421 gtggctgctg ttgggccttg ctttgctctt cagtttagta ttatttatgt acctcctgga
      481 atgtgccccc cagactgatg gaaatgcttc tcttcctggt gttgttagag aaaattatgg
      541 taaagaatat taccaggccc tcctgcagga gcaagaagaa cattaccaaa ccagggcaac
      601 cagtctgaaa cgccagattg cccagctaaa gcaagaatta caagatatga gtgagaagat
      661 gagagccttg caagagagaa agaagctagg ggctaacggc gtaggctatc ctggcaacag
      721 agagcaggca cccagtgacc tcttagagtt tcttcactcc cagatcgata gagctgaagt
      781 tagcgtgggg gccaaactcc ccagtgagta tggagtcgtt ccctttgaaa gttttacttt
      841 aatgaaagta tttcagttgg aaatgggtct cactcgccat cctgaagaaa agccagttag
      901 aaaagacaaa cgagacgaac tggtagaagt tattgaagct ggcgtggagg tcattaataa
      961 tcctgatgaa gatgatgcac aggaagatga ggagggtccc cttggagaga aactgatatt
     1021 taatgaaaat gacttcatag aaggctatta tcgcactgag agagataaag gcacgcagta
     1081 tgaactgttt tttaagaaag cagaccttat ggagtacaga catgtgaccc tcttccgccc
     1141 ttttggacct ctcatgaaag tgaagaatga actgatcgac attacaagat cagttattaa
     1201 tatcattgtg ccacttgcgg agaggacaga ggcgttttca cagtttatgc agaacttcag
     1261 agatgtttgt attcatcaag acaagaggat tcatctcacc gttgtgtatt ttgggaaaga
     1321 aggactatct aaagtcaagt ctattctaga atctgtctca agtgagtctg attttcacaa
     1381 ttacaccttg gtctcgttgg acgaagaatt taatcgtgga cgaggactaa atgtgggtgc
     1441 ccgagcttgg gacaagggag aggtcttgat gtttttctgt gatgttgata tatatttctc
     1501 agctgaattc cttaacagct gccggttaaa tgctgagcca ggtaaaaagg tgttttaccc
     1561 tgtggtgttc agtctttaca accctgccat tgtctatgcc aaccaggacg tgccgccccc
     1621 tgtggagcag cagctggttc ataaaaagga ctctggtttt tggagagact ttggctttgg
     1681 gatgacctgt caatatcaat cggatttcct gagtgtcggt ggattcgaca tggaagtaaa
     1741 aggctggggt ggagaagatg ttcatcttta ccgaaaatac ctacacggtg atcttattgt
     1801 gattcggact ccagttcccg gtcttttcca cctctggcat gagaaacatt gtgcagatga
     1861 gctgacccct gagcagtacc gaatgtgcat ccaatccaaa gccatgaatg aggcctccca
     1921 ctctcacctg ggaatgatgg ttttcaggga ggagatagag atgcatcttc gcaaacaggc
     1981 atacagaaca aacagcgaga ctgctgggta acagtcaacg tgggagagtc ggaagcacaa
     2041 accagcactg ttaattcagc cttaattcca actctagatt atgtgcctct tccagagaaa
     2101 aacttttctt ttccatgttc tttctgaaat gtttttgtag ctcaacttga tgtaagaaga
     2161 aaacgcaggt gtcttgatgt atagcctgcc ttgtgagagt actatagtat gaaacagttg
     2221 acaaaatgaa attttatatt tgtccccaaa atcgtttgaa ttagattctg cctggtacct
     2281 gtgttctgat tggtcctgga gtatacgagg ctaatgttga gttggtcagc acactcctac
     2341 agggaacaga acactggttg gtcgaaatgt tcaggcagat atgcctgctg accactaagt
     2401 caatcatttg acttcagtct ttagatgaaa gctttcattg atcacatgta gaatattttt
     2461 tttcacagaa aataattaac atatctaaaa tctacagtcc atcacaaagg cataagtcaa
     2521 aaccccttag tttcatgttg ctttcccttt gaatatgttt ttaagcaaat ggatatagta
     2581 gatatagttt agaatttatg gtttgattat aaataaaata tattgttatg ttaccaatgt
     2641 tcaaaattca tagtcaaatt gtagtagatt gttttgaaca ttttattata ttggaatgca
     2701 ggacagtagc tgtgtttggg aacgtctttt tatataagcc agaggcatag aagatatgaa
     2761 cgttatagca tggttttgta tggcttttgt tttgcactgt cttgtttagc atgggcacat
     2821 atatatttat tttctatatg ctaattttgc cataattgtg tttgcatttg tattatactt
     2881 tcagttgaaa gagttttatt gaaaaaaaat taaatgcatt atataataag accacccttg
     2941 atttgagaaa agggaaggaa ggaaaagtga cctgataaaa atataaatta tggagtactt
     3001 taaaaaaatg tacagattga atgctacttt aattgaaatg taaattatat taatgagaca
     3061 agatgaataa gaaattatat tccagtagaa ctgtactata atatttatca cacatggata
     3121 ttttatcatt gggtcattta ctagatccag aaattggaga taaaataaag gcatgcatag
     3181 tttttttaac ccctgacttt taaagggacc tgctatttct atataaccca tgagaactca
     3241 gaaaaagtca ttatttttgt tcagtaaatc ataccatctc aaaagttaca aatgccaaat
     3301 ttctaggtgc taatgtgaat ccgattcaat aataactagt tgctgagggt tctacccggc
     3361 atatcgttga ggtatttttt aagatgtata aagtgctttc tgcagaataa gcagagccag
     3421 tatctccttt gtacacactt atttattgta aagaccagtg accatggtat ttaaaattag
     3481 ggtacttaat gattttcaaa ggtgatttca gcttggtttt gagataatca aatgattttt
     3541 actttaactc ctattttttt aaactagctt ttatttgaag atggaagcta agcaatgaaa
     3601 gcattactat attttttaca ttaagtggta ccaataaagt attttgttac caaaagctaa
     3661 aaaaaaaaaa aaaaaa
//
