LOCUS       NM_172753               3943 bp    mRNA    linear   ROD 04-JUN-2024
DEFINITION  Mus musculus chondroitin sulfate N-acetylgalactosaminyltransferase
            1 (Csgalnact1), transcript variant 2, mRNA.
ACCESSION   NM_172753
VERSION     NM_172753.5
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3943)
  AUTHORS   Inada,R., Miyamoto,K., Tanaka,N., Moriguchi,K., Kadomatsu,K.,
            Takeuchi,K., Igarashi,M. and Kusunoki,S.
  TITLE     Chondroitin sulfate N-acetylgalactosyltransferase-1 knockout shows
            milder phenotype in experimental autoimmune encephalomyelitis than
            in wild type
  JOURNAL   Glycobiology 31 (3), 260-265 (2021)
   PUBMED   32839819
  REMARK    GeneRIF: Chondroitin sulfate N-acetylgalactosyltransferase-1
            knockout shows milder phenotype in experimental autoimmune
            encephalomyelitis than in wild type.
REFERENCE   2  (bases 1 to 3943)
  AUTHORS   Katagiri,T., Uemura,S., Ushiki,T., Nakajima-Takagi,Y., Oshima,M.,
            Mikami,T., Kawasaki,A., Ishiguro,H., Tanaka,T., Sone,H.,
            Kitagawa,H., Igarashi,M., Iwama,A. and Masuko,M.
  TITLE     Distinct effects of chondroitin sulfate on hematopoietic cells and
            the stromal microenvironment in bone marrow hematopoiesis
  JOURNAL   Exp Hematol 96, 52-62 (2021)
   PUBMED   33582241
REFERENCE   3  (bases 1 to 3943)
  AUTHORS   Bachvarova,V., Dierker,T., Esko,J., Hoffmann,D., Kjellen,L. and
            Vortkamp,A.
  TITLE     Chondrocytes respond to an altered heparan sulfate composition with
            distinct changes of heparan sulfate structure and increased levels
            of chondroitin sulfate
  JOURNAL   Matrix Biol 93, 43-59 (2020)
   PUBMED   32201365
REFERENCE   4  (bases 1 to 3943)
  AUTHORS   Ida-Yonemochi,H., Morita,W., Sugiura,N., Kawakami,R., Morioka,Y.,
            Takeuchi,Y., Sato,T., Shibata,S., Watanabe,H., Imamura,T.,
            Igarashi,M., Ohshima,H. and Takeuchi,K.
  TITLE     Craniofacial abnormality with skeletal dysplasia in mice lacking
            chondroitin sulfate N-acetylgalactosaminyltransferase-1
  JOURNAL   Sci Rep 8 (1), 17134 (2018)
   PUBMED   30459452
  REMARK    GeneRIF: CSGalNAcT1 plays important roles in connective tissue
            organization and following intramembranous ossification of the
            craniofacial skeleton.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 3943)
  AUTHORS   Uijtdewilligen,P.J.E., Versteeg,E.M., van de Westerlo,E.M.A., van
            der Vlag,J., Daamen,W.F. and van Kuppevelt,T.H.
  TITLE     Dynamic Expression of Genes Involved in
            Proteoglycan/Glycosaminoglycan Metabolism during Skin Development
  JOURNAL   Biomed Res Int 2018, 9873471 (2018)
   PUBMED   30228991
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 3943)
  AUTHORS   Watanabe,Y., Takeuchi,K., Higa Onaga,S., Sato,M., Tsujita,M.,
            Abe,M., Natsume,R., Li,M., Furuichi,T., Saeki,M., Izumikawa,T.,
            Hasegawa,A., Yokoyama,M., Ikegawa,S., Sakimura,K., Amizuka,N.,
            Kitagawa,H. and Igarashi,M.
  TITLE     Chondroitin sulfate N-acetylgalactosaminyltransferase-1 is required
            for normal cartilage development
  JOURNAL   Biochem J 432 (1), 47-55 (2010)
   PUBMED   20812917
  REMARK    GeneRIF: Results suggest that CSGalNAcT1 is required for normal CS
            production in developing cartilage.
REFERENCE   7  (bases 1 to 3943)
  AUTHORS   Tang,T., Li,L., Tang,J., Li,Y., Lin,W.Y., Martin,F., Grant,D.,
            Solloway,M., Parker,L., Ye,W., Forrest,W., Ghilardi,N., Oravecz,T.,
            Platt,K.A., Rice,D.S., Hansen,G.M., Abuin,A., Eberhart,D.E.,
            Godowski,P., Holt,K.H., Peterson,A., Zambrowicz,B.P. and de
            Sauvage,F.J.
  TITLE     A mouse knockout library for secreted and transmembrane proteins
  JOURNAL   Nat Biotechnol 28 (7), 749-755 (2010)
   PUBMED   20562862
REFERENCE   8  (bases 1 to 3943)
  AUTHORS   Bouma,G.J., Hudson,Q.J., Washburn,L.L. and Eicher,E.M.
  TITLE     New candidate genes identified for controlling mouse gonadal sex
            determination and the early stages of granulosa and Sertoli cell
            differentiation
  JOURNAL   Biol Reprod 82 (2), 380-389 (2010)
   PUBMED   19864314
REFERENCE   9  (bases 1 to 3943)
  AUTHORS   Sakai,K., Kimata,K., Sato,T., Gotoh,M., Narimatsu,H., Shinomiya,K.
            and Watanabe,H.
  TITLE     Chondroitin sulfate N-acetylgalactosaminyltransferase-1 plays a
            critical role in chondroitin sulfate synthesis in cartilage
  JOURNAL   J Biol Chem 282 (6), 4152-4161 (2007)
   PUBMED   17145758
REFERENCE   10 (bases 1 to 3943)
  AUTHORS   Silbert,J.E. and Freilich,L.S.
  TITLE     Biosynthesis of chondroitin sulphate by a Golgi-apparatus-enriched
            preparation from cultures of mouse mastocytoma cells
  JOURNAL   Biochem J 190 (2), 307-313 (1980)
   PUBMED   6781479
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BY289912.1, AK080687.1 and AC117740.12.
            
            On Nov 22, 2011 this sequence version replaced NM_172753.4.
            
            Transcript Variant: This variant (2) differs in the 5' UTR compared
            to variant 1. Both variants 1 and 2 encode the same protein.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC089162.1, AK080687.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164131, SAMN01164134
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-32                BY289912.1         2-33
            33-2742             AK080687.1         2-2711
            2743-3943           AC117740.12        33790-34990
FEATURES             Location/Qualifiers
     source          1..3943
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="8"
                     /map="8 33.88 cM"
     gene            1..3943
                     /gene="Csgalnact1"
                     /gene_synonym="4732435N03Rik; CSGalNAcT-1"
                     /note="chondroitin sulfate
                     N-acetylgalactosaminyltransferase 1"
                     /db_xref="GeneID:234356"
                     /db_xref="MGI:MGI:2442354"
     exon            1..121
                     /gene="Csgalnact1"
                     /gene_synonym="4732435N03Rik; CSGalNAcT-1"
                     /inference="alignment:Splign:2.1.0"
     exon            122..258
                     /gene="Csgalnact1"
                     /gene_synonym="4732435N03Rik; CSGalNAcT-1"
                     /inference="alignment:Splign:2.1.0"
     exon            259..407
                     /gene="Csgalnact1"
                     /gene_synonym="4732435N03Rik; CSGalNAcT-1"
                     /inference="alignment:Splign:2.1.0"
     exon            408..1335
                     /gene="Csgalnact1"
                     /gene_synonym="4732435N03Rik; CSGalNAcT-1"
                     /inference="alignment:Splign:2.1.0"
     CDS             708..2300
                     /gene="Csgalnact1"
                     /gene_synonym="4732435N03Rik; CSGalNAcT-1"
                     /EC_number="2.4.1.174"
                     /note="beta1,4 N-acetylgalactosaminyltransferase;
                     beta4GalNAcT-1; chondroitin
                     beta-1,4-N-acetylgalactosaminyltransferase 1"
                     /codon_start=1
                     /product="chondroitin sulfate
                     N-acetylgalactosaminyltransferase 1"
                     /protein_id="NP_766341.4"
                     /db_xref="CCDS:CCDS22341.1"
                     /db_xref="GeneID:234356"
                     /db_xref="MGI:MGI:2442354"
                     /translation="MVRRGLLGWISRVVILLVLLCCAISVLYMLACTPKGDQEQLGLP
                     RANGPTGKDGYQAVLQEREEQHRNYVNSLKRQIAQLKDELQARSEQFRSGQDQASDAT
                     SLRSGWDREPKAQADLLAFLRGQVDKAEVHAGVKLATEYAAVPFDSFTLQKVYQLETG
                     LTRHPEEKPVRKDKRDELVEAIESALESLNSPVESSPHQRPYTAADFIEGIYRTERDK
                     GTLYELTFKGDHKHEFQRLVLFRPFGPIMKVKKEKLNLANTLINVIVPLARRVDKFRH
                     FMQNFREMCIQQDGRVHLTVVYFGKEEMNEVKGILENTSKAANFRNFTFIQLNGEFSR
                     GKGLDVGARFWKGSNVLLFFCDVDIYFTSEFLNTCRLNTQPGKKVFYPVLFSQYNPGV
                     IYGHHDAVPPLGQQLVIKKETGFWRDFGFGMTCQYRSDFINIGGFDLDIKGWGGEDVH
                     LYRKYLHSNLIVVRTPVRGLFHLWHEKHCMDELTPEQYKMCMQSKAMNEASHGQLGML
                     VFRHEIEAHLRKQKQKASSKKT"
     exon            1336..1552
                     /gene="Csgalnact1"
                     /gene_synonym="4732435N03Rik; CSGalNAcT-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1553..1654
                     /gene="Csgalnact1"
                     /gene_synonym="4732435N03Rik; CSGalNAcT-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1655..1833
                     /gene="Csgalnact1"
                     /gene_synonym="4732435N03Rik; CSGalNAcT-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1834..1928
                     /gene="Csgalnact1"
                     /gene_synonym="4732435N03Rik; CSGalNAcT-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1929..2010
                     /gene="Csgalnact1"
                     /gene_synonym="4732435N03Rik; CSGalNAcT-1"
                     /inference="alignment:Splign:2.1.0"
     exon            2011..3943
                     /gene="Csgalnact1"
                     /gene_synonym="4732435N03Rik; CSGalNAcT-1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3911..3916
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Csgalnact1"
                     /gene_synonym="4732435N03Rik; CSGalNAcT-1"
                     /note="hexamer: AATAAA"
     polyA_site      3930
                     /gene="Csgalnact1"
                     /gene_synonym="4732435N03Rik; CSGalNAcT-1"
                     /note="major polyA site"
ORIGIN      
        1 ggctgttgga actggaggtg gagccgagcc cagggccgcc tcgcaggagg gaaagttcag
       61 cggcagcagg ggagcagcgt agccgaggga tccggctccg ctgtctgcag tgttgcctgg
      121 ggttccttga acatgcatga cagattggag agttgccatg tggatctaca gtcctcagtg
      181 tgagaagctt taaacaatca tttctgtggc tggaatcttc aaatgatcta ctttttattg
      241 cctctggagc ataaccagtg gtggcgctga gctgaacacg gtgctagcag aagggggcgg
      301 tactttccct ctggaccctg acttggaagc caagtgcaac tccagcgcag tcaccaccag
      361 aagcagatgt gcgagcggaa cagctgtgcg cctttctttc tgccgaggtt ccctctcatc
      421 ttcagcatat cagaaaggct atgatgggaa cgaatgaagg aacttggagg ctccacttga
      481 ctgactgttt ctgtctagaa aatcttccct tggaagcaag agagccgtct catgtaatgg
      541 ctgacttcta aagacaggcc atgttctaga aggaaatagg tgaaattggt tctgggaact
      601 tcagtggcac tgtggtctaa ccagaaaagc ccgtgttggg ggcccttctg gtgccctggg
      661 atgttgcata ctctggacct ggatgcatgg cctgcacctg agtgagaatg gtccgccgcg
      721 ggctgctggg gtggatttct cgggtagtga ttctgctggt gctgctctgt tgtgccatct
      781 ctgtcctcta catgttagcc tgcactccaa aaggcgacca ggagcagttg ggactgccac
      841 gggccaatgg acccacaggc aaagatggct accaagcggt gctgcaggag cgcgaggaac
      901 agcatcgtaa ctatgtgaat agccttaaac gacagatagc tcagctaaag gatgaactgc
      961 aggcacgcag tgagcagttc cgcagtgggc aggaccaggc cagcgatgcc accagcctgc
     1021 gctcaggctg ggaccgtgag cccaaagccc aggccgatct gctggccttc ctgcgtggac
     1081 aggtggacaa ggccgaggta catgccggtg tcaagctggc cacggagtat gctgctgtgc
     1141 cttttgatag cttcactctg cagaaagtat accagctgga gactggcctg acccgccacc
     1201 ctgaggagaa gccagtgagg aaagacaagc gcgatgagct ggtagaagcc atcgaatcgg
     1261 ccctggagag tctaaacagc cctgtggaga gcagcccaca ccagcgtcct tacacagctg
     1321 cagacttcat agaagggatt taccgaaccg aaagggataa aggcactttg tatgagctga
     1381 ccttcaaagg ggaccacaag catgaattcc agcgacttgt cctatttcga ccttttggcc
     1441 ccatcatgaa agtgaaaaag gaaaaactca acctggccaa cacgcttatc aatgttattg
     1501 tgcccctagc gaggagggtg gacaagttcc ggcacttcat gcagaacttc agggagatgt
     1561 gcatccaaca ggatgggaga gttcatctca ccgttgttta ttttgggaaa gaagaaatga
     1621 atgaagtcaa aggaatactt gaaaacactt caaaagctgc caatttcaga aacttcacct
     1681 tcatccaact gaatggagaa ttctcccggg gaaagggact ggatgttgga gcccgcttct
     1741 ggaagggaag taacgtcctg ctctttttct gtgatgtaga catctacttc acctcggagt
     1801 tcctcaacac ttgtaggctg aacacacagc cagggaagaa ggtattttat ccggttctgt
     1861 tcagtcagta taaccccggc gtaatctacg gccatcacga tgcagtccct ccgctaggac
     1921 agcagctggt cataaagaag gaaacaggat tttggaggga ctttggattt gggatgacat
     1981 gtcagtaccg gtcagacttc atcaacatag gtggatttga cctggacatc aaaggctggg
     2041 gtggtgaaga tgtgcacctg taccggaaat atctccatag caacctcata gtggtccgca
     2101 cacctgtacg gggacttttc cacctgtggc atgaaaagca ctgtatggat gaactgaccc
     2161 ctgagcagta caagatgtgc atgcaatcaa aggctatgaa tgaagcatcc catgggcagc
     2221 tggggatgct tgtcttccgg catgaaatag aggctcatct tcgcaaacag aagcagaaag
     2281 ccagcagtaa aaagacatga tctcctaggc acagattagc atagacattt aatttttttt
     2341 gcctttggca gttgctgaag aagtagatgc aacaaagagc aacaaagtat ctgactggtg
     2401 agggagagat gaaagaagag tctcatcccg ggattctgtt ggctccgtgt atggtgacgc
     2461 tgactgtgtt ttcagcaaat gatctctctg gttcctgctc actgaaatat tcacaacctc
     2521 agaccagttt tgtagaacgt ttactagaat gagatgcaaa cacattgaat tggttacatc
     2581 agagggcttt ggtactacca ggactaccaa agtctcagac caagcagagg atctatgtga
     2641 gaaatggaga tctctgacat tagcgtcctc caggagatag tgagcaaaat caaagggccc
     2701 tggaaacaca ccatggaaac tgtaatttcc ccagtattaa ccaaaaaaac aaaaaaacaa
     2761 aaccaccacc accaacaaca aaaaaccctc tctttcccat tattgtttag tcatctttat
     2821 tctaaaaatg cacttttgtt tttgcttctg agtgttatgc ttatttaatt acccatttgc
     2881 aagccttagt aggaacatat tgtagtatat acaattctat gattttttaa aaaagatttt
     2941 attttagttt ttgttgtttt tgttgatttg tctacttgag acagtcttgt cacagagccc
     3001 gagataaact tgaaattatg atcctcctgc ctctgaagcc tgagtgctga gattaaaggt
     3061 gggtgccacc acagctggca agaagataca tgcagaggag tgggaggaaa tcctctagtt
     3121 gcaagctcct ggagatctga tctaaggact ggccaatctc agaccacgct aaggctatgt
     3181 gtggggttgg ggagagaaag gtatcaagaa actatctaag actgtccaaa gaggaacaat
     3241 caaacactgg agaaagaaaa agacactttc ctcttcacaa aaaggacact aagaagccct
     3301 tggtatattt actagagaac ataatttctc ctctggagaa gtggggacca ctttcttacc
     3361 tgtttgaata aaccaaagtt atgaaccaaa caatcacttt tcaaaacagg gtgctcttct
     3421 tggtctctgg tttgcataag aagaaaacgc aggaaatata tatctaaata tatattttga
     3481 aagaccaatc cgttaatcta gtgagacgga agattttgct acatttccat tcactcctgg
     3541 ctatgtggga gcaacttatt tttaaaataa gcaatgttac tgattgctgg gacgtttctg
     3601 aagcattttc ttataactcc aaattctttt tttttaaata ataaaatctg attaacattg
     3661 agtggtttct acatccatat gcaaactatt tatttattag ccagcaccgg atgcatgagc
     3721 tgatcatctc tcctgtctct gtgccttctg tctgcccaca gtgaactcat tgttgaaatg
     3781 cttcaagaac actcaagctg tgtgttataa aaaaaaaaaa aggaaaaaga gcattgtatt
     3841 gatttgtact ggtagtttat aaaatttaat taaaacacaa gacatgaatg gaaggtggca
     3901 ttgcacagct aataaaatct gtgaatttga tttctttccg taa
//
