LOCUS       NM_026444               2954 bp    mRNA    linear   ROD 01-AUG-2024
DEFINITION  Mus musculus citrate synthase (Cs), mRNA; nuclear gene for
            mitochondrial product.
ACCESSION   NM_026444
VERSION     NM_026444.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2954)
  AUTHORS   Ayyangar,U., Karkhanis,A., Tay,H., Afandi,A.F.B., Bhattacharjee,O.,
            Ks,L., Lee,S.H., Chan,J. and Raghavan,S.
  TITLE     Metabolic rewiring of macrophages by epidermal-derived lactate
            promotes sterile inflammation in the murine skin
  JOURNAL   EMBO J 43 (7), 1113-1134 (2024)
   PUBMED   38418556
REFERENCE   2  (bases 1 to 2954)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 2954)
  AUTHORS   Beutner,G., Burris,J.R., Collins,M.P., Kulkarni,C.A.,
            Nadtochiy,S.M., de Mesy Bentley,K.L., Cohen,E.D., Brookes,P.S. and
            Porter,G.A. Jr.
  TITLE     Coordinated metabolic responses to cyclophilin D deletion in the
            developing heart
  JOURNAL   iScience 27 (3), 109157 (2024)
   PUBMED   38414851
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2954)
  AUTHORS   Khadhraoui,N., Prola,A., Vandestienne,A., Blondelle,J.,
            Guillaud,L., Courtin,G., Bodak,M., Prost,B., Huet,H.,
            Wintrebert,M., Pechoux,C., Solgadi,A., Relaix,F., Tiret,L. and
            Pilot-Storck,F.
  TITLE     Hacd2 deficiency in mice leads to an early and lethal mitochondrial
            disease
  JOURNAL   Mol Metab 69, 101677 (2023)
   PUBMED   36693621
REFERENCE   5  (bases 1 to 2954)
  AUTHORS   Li,Z., Liu,R., Liu,Y., Zhao,M., Luan,J., Wang,Y., Shang,W.,
            Song,X., Sun,Y. and Han,F.
  TITLE     H55 N variation in citrate synthase leads to decrement in the
            enzyme activity and transport rate to mitochondria in HEI-OC1 cells
  JOURNAL   Biochem Biophys Res Commun 612, 134-140 (2022)
   PUBMED   35525197
  REMARK    GeneRIF: H55 N variation in citrate synthase leads to decrement in
            the enzyme activity and transport rate to mitochondria in HEI-OC1
            cells.
REFERENCE   6  (bases 1 to 2954)
  AUTHORS   Chow,K.M., Ma,Z., Cai,J., Pierce,W.M. and Hersh,L.B.
  TITLE     Nardilysin facilitates complex formation between mitochondrial
            malate dehydrogenase and citrate synthase
  JOURNAL   Biochim Biophys Acta 1723 (1-3), 292-301 (2005)
   PUBMED   15809022
REFERENCE   7  (bases 1 to 2954)
  AUTHORS   Mootha,V.K., Bunkenborg,J., Olsen,J.V., Hjerrild,M.,
            Wisniewski,J.R., Stahl,E., Bolouri,M.S., Ray,H.N., Sihag,S.,
            Kamal,M., Patterson,N., Lander,E.S. and Mann,M.
  TITLE     Integrated analysis of protein composition, tissue diversity, and
            gene regulation in mouse mitochondria
  JOURNAL   Cell 115 (5), 629-640 (2003)
   PUBMED   14651853
REFERENCE   8  (bases 1 to 2954)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
REFERENCE   9  (bases 1 to 2954)
  AUTHORS   Jiang,C.H., Tsien,J.Z., Schultz,P.G. and Hu,Y.
  TITLE     The effects of aging on gene expression in the hypothalamus and
            cortex of mice
  JOURNAL   Proc Natl Acad Sci U S A 98 (4), 1930-1934 (2001)
   PUBMED   11172053
REFERENCE   10 (bases 1 to 2954)
  AUTHORS   Lahbib-Mansais,Y., Yerle,M., Pinton,P. and Gellin,J.
  TITLE     Chromosomal localization of homeobox genes and associated markers
            on porcine chromosomes 3, 5, 12, 15, 16 and 18: comparative mapping
            study with human and mouse
  JOURNAL   Mamm Genome 7 (3), 174-179 (1996)
   PUBMED   8833235
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from CJ144772.1, AC090489.8 and
            BU700437.1.
            
            On Jul 27, 2016 this sequence version replaced NM_026444.3.
            
            Summary: The protein encoded by this gene is a central metabolic
            pathway enzyme, catalyzing the first step of the tricarboxylic acid
            cycle in which acetyl coenzyme A and oxaloacetate are converted to
            citrate and coenzyme A. This enzyme is found in nearly all cells
            capable of oxidative metabolism. This protein is nuclear encoded
            and transported into the mitochondrial matrix, where the mature
            form is found. [provided by RefSeq, Jul 2016].
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC029754.1, AK149990.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            RefSeq Select criteria             :: based on conservation,
                                                  expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-105               CJ144772.1         2-106
            106-233             AC090489.8         87557-87684
            234-284             AC090489.8         99478-99528
            285-392             AC090489.8         100158-100265
            393-458             AC090489.8         100924-100989
            459-590             AC090489.8         102399-102530
            591-779             AC090489.8         102748-102936
            780-979             AC090489.8         108059-108258
            980-1109            AC090489.8         108895-109024
            1110-1211           AC090489.8         109159-109260
            1212-1421           AC090489.8         109669-109878
            1422-2932           AC090489.8         110693-112203
            2933-2954           BU700437.1         1-22                c
FEATURES             Location/Qualifiers
     source          1..2954
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="10"
                     /map="10 76.53 cM"
     gene            1..2954
                     /gene="Cs"
                     /gene_synonym="2610511A05Rik; 9030605P22Rik; Ahl4; Cis"
                     /note="citrate synthase"
                     /db_xref="GeneID:12974"
                     /db_xref="MGI:MGI:88529"
     exon            1..233
                     /gene="Cs"
                     /gene_synonym="2610511A05Rik; 9030605P22Rik; Ahl4; Cis"
                     /inference="alignment:Splign:2.1.0"
     CDS             192..1586
                     /gene="Cs"
                     /gene_synonym="2610511A05Rik; 9030605P22Rik; Ahl4; Cis"
                     /EC_number="2.3.3.1"
                     /note="citrate synthase, mitochondrial; citrate
                     (Si)-synthase"
                     /codon_start=1
                     /product="citrate synthase, mitochondrial precursor"
                     /protein_id="NP_080720.1"
                     /db_xref="CCDS:CCDS24273.1"
                     /db_xref="GeneID:12974"
                     /db_xref="MGI:MGI:88529"
                     /translation="MALLTAATRLLGAKNSSCLVLAARHASASSTNLKDVLSNLIPKE
                     QARIKTFKQQHGKTVVGQITVDMMYGGMRGMKGLVYETSVLDPDEGIRFRGYSIPECQ
                     KMLPKAKGGEEPLPEGLFWLLVTGQMPTEEQVSWLSREWAKRAALPSHVVTMLDNFPT
                     NLHPMSQLSAAITALNSESNFARAYAEGMNRAKYWELIYEDCMDLIAKLPCVAAKIYR
                     NLYREGSSIGAIDSRLDWSHNFTNMLGYTDPQFTELMRLYLTIHSDHEGGNVSAHTSH
                     LVGSALSDPYLSFAAAMNGLAGPLHGLANQEVLVWLTQLQKEVGKDVSDEKLRDYIWN
                     TLNSGRVVPGYGHAVLRKTDPRYSCQREFALKHLPKDPMFKLVAQLYKIVPNILLEQG
                     KAKNPWPNVDAHSGVLLQYYGMTEMNYYTVLFGVSRALGVLAQLIWSRALGFPLERPK
                     SMSTDGLMKFVDSK"
     exon            234..284
                     /gene="Cs"
                     /gene_synonym="2610511A05Rik; 9030605P22Rik; Ahl4; Cis"
                     /inference="alignment:Splign:2.1.0"
     exon            285..392
                     /gene="Cs"
                     /gene_synonym="2610511A05Rik; 9030605P22Rik; Ahl4; Cis"
                     /inference="alignment:Splign:2.1.0"
     exon            393..458
                     /gene="Cs"
                     /gene_synonym="2610511A05Rik; 9030605P22Rik; Ahl4; Cis"
                     /inference="alignment:Splign:2.1.0"
     exon            459..590
                     /gene="Cs"
                     /gene_synonym="2610511A05Rik; 9030605P22Rik; Ahl4; Cis"
                     /inference="alignment:Splign:2.1.0"
     exon            591..779
                     /gene="Cs"
                     /gene_synonym="2610511A05Rik; 9030605P22Rik; Ahl4; Cis"
                     /inference="alignment:Splign:2.1.0"
     exon            780..979
                     /gene="Cs"
                     /gene_synonym="2610511A05Rik; 9030605P22Rik; Ahl4; Cis"
                     /inference="alignment:Splign:2.1.0"
     exon            980..1109
                     /gene="Cs"
                     /gene_synonym="2610511A05Rik; 9030605P22Rik; Ahl4; Cis"
                     /inference="alignment:Splign:2.1.0"
     exon            1110..1211
                     /gene="Cs"
                     /gene_synonym="2610511A05Rik; 9030605P22Rik; Ahl4; Cis"
                     /inference="alignment:Splign:2.1.0"
     exon            1212..1421
                     /gene="Cs"
                     /gene_synonym="2610511A05Rik; 9030605P22Rik; Ahl4; Cis"
                     /inference="alignment:Splign:2.1.0"
     exon            1422..2936
                     /gene="Cs"
                     /gene_synonym="2610511A05Rik; 9030605P22Rik; Ahl4; Cis"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2911..2916
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Cs"
                     /gene_synonym="2610511A05Rik; 9030605P22Rik; Ahl4; Cis"
                     /note="hexamer: AATAAA"
     polyA_site      2931
                     /gene="Cs"
                     /gene_synonym="2610511A05Rik; 9030605P22Rik; Ahl4; Cis"
                     /note="major polyA site"
ORIGIN      
        1 agagggcggg gcctcgccga ggaccctggg ctgggcgcct gtgccggttt gtctaccctt
       61 cccctcagcc gcctcctttc aaccttgtcc gccggttggc gcggtctcca tcacagcggc
      121 gaccgctcct gttgcagctg tagctctctc ccttcggtcc cttcccgcca ggtcccggag
      181 tttcgcccgc catggctcta ctcactgcag caacccggct cttgggagcc aagaactcat
      241 cctgcctcgt ccttgctgcc cggcatgcca gtgcttcttc cacgaatttg aaagatgtac
      301 tgagcaatct gatacctaag gagcaggcca gaattaagac cttcaagcag caacatggga
      361 agacagtggt gggccagatc actgtggaca tgatgtacgg tggcatgaga ggcatgaagg
      421 gacttgtgta tgagacttcg gttcttgatc ctgacgaggg catccgtttc cgaggctaca
      481 gtatccctga gtgccagaaa atgctgccta aggctaaggg tggagaagaa cccctgcctg
      541 agggcttatt ttggctgctg gtaactggac agatgcccac agaggaacag gtgtcttggc
      601 tctcacgaga atgggcaaaa agggcagctc tgccttctca tgtggtcacc atgctggaca
      661 attttccaac caatctgcac cctatgtctc agctcagtgc agccatcaca gccctcaaca
      721 gtgaaagcaa cttcgcccgg gcatatgcag agggaatgaa ccgagctaag tactgggagc
      781 tcatctatga ggactgcatg gacctcattg ccaagctgcc gtgcgttgca gccaagatct
      841 accggaatct gtaccgggag ggcagcagta tcggagccat tgactctagg ctagactggt
      901 cacacaattt taccaacatg ttaggctaca ccgaccctca gttcaccgag ctcatgcgtt
      961 tgtacctcac catccatagt gaccatgagg gtggtaatgt aagtgcccac acaagccatt
     1021 tggtgggcag cgccctttca gacccttacc tgtcctttgc agcagccatg aatgggctgg
     1081 cggggcctct acatggacta gcaaatcagg aggtgcttgt ctggctgaca cagctacaga
     1141 aggaagttgg caaagacgtg tcagatgaga agttacgaga ctacatctgg aacacactca
     1201 attcaggacg ggtggtccca ggatacggtc atgcagtact gaggaagact gaccctcgct
     1261 attcctgtca gcgagagttt gctctgaaac atctgcctaa ggatcccatg ttcaagctgg
     1321 tggctcagct gtacaagatt gtgcccaata tcctcttaga gcaagggaag gctaagaacc
     1381 cttggcccaa cgtagacgct cacagtgggg tgctgctcca gtactatggc atgacggaga
     1441 tgaactacta cacagtcctg tttggagtgt ctcgggcact gggtgtgcta gcccagctca
     1501 tctggagcag agccctaggc ttccccctgg aaaggcccaa gtccatgagc acggatggcc
     1561 tgatgaagtt tgtggactct aagtaagggt aaaatgggag actgggggga aactgactat
     1621 aagaaaacga ggaagcttaa aaataaagtg taattttgtt ttgtttcagg ggcctttaag
     1681 acttaagatt aaactgtatc tgaggcactg aaaatatatt tgaagttaaa atataaatta
     1741 agacttgaaa gacaaaagtg accccttctc ccctcaccag ttcacctccc cctcctggta
     1801 tgagctgccc agcagctgta ggatgaccgg gactgaagca tgtggtcagg gttagatctg
     1861 cctaccacca tctctagagc aagactcagg ctcctcctct ttaggtcaga gcaggttaca
     1921 gacaagctgc agtcactcat ggagctttgt ttctatgctg ccacccccaa gtcatctacg
     1981 ccagggacag gcccctttct ttccatagaa atcatgttgg atgccagctg tgtccaacac
     2041 tagggccttt cccaatgtac acaaacacct cctacctagc aagatctctt ggttagctgg
     2101 agacgctttg gcaacttttt atgctatcag atgatcttaa atggcgtagt atttattgtg
     2161 actggcaccc aacatttgag ttatttctcc ttttaaattt ataccattaa agttaaggtc
     2221 tggggagtta tgtttccttc caggcctcta tacctgctgt gcctcaagct gaggatgctg
     2281 ggggtctccc tgtcctgaac tccactaggg tcctctctca gcaggtctgt ccgacctgtg
     2341 gtcttcctgg tcgtttggct ttatcagtac ttaatgtgaa ctaggtcttt tacttccatg
     2401 gaacataagc cactatcttc tgaccttggt ttctcctgtt aatatgctct ggcacggaac
     2461 aagggctcag gaatgaccag cctcttcggg cctggtccca ggggttctag cttttctttt
     2521 tgaacagcag ttacacacag ttcccactga atatcagatg gatggaccta gggcttgggg
     2581 ccttcttctc atcccgtgtc aaggaaacgg tcctttattt atcagtgtct tttgctcttc
     2641 ccttaagtaa gagcccatag cacagcactt attttagcag ccccatttgc acagattttc
     2701 agtgactcag aatgctctac tgccttttct ttgagaaagg gttgagatac actcctgtcc
     2761 tgcccctcct catcccaaag ctcctgcctg tgtttgtatg gaacccaact ccagcaccac
     2821 cacactgttg agatggacac actgtaaacc cctgggtaac tgttaaagtc atgatgcaga
     2881 cttcaggatg ttctgtataa aattaaaaat aataaatgtt tttattaaca atgtttaaaa
     2941 aaaaaaaaaa aaaa
//
