LOCUS       NM_021352                854 bp    mRNA    linear   ROD 10-FEB-2025
DEFINITION  Mus musculus crystallin, beta B3 (Crybb3), transcript variant 1,
            mRNA.
ACCESSION   NM_021352 NM_001359009
VERSION     NM_021352.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 854)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 854)
  AUTHORS   Yu,W., Yu,Z., Wu,D., Zhang,J., Zhu,Y., Zhang,Y., Ning,H., Wang,M.,
            Zhang,J. and Zhao,J.
  TITLE     Lens-specific conditional knockout of Msx2 in mice leads to ocular
            anterior segment dysgenesis via activation of a calcium signaling
            pathway
  JOURNAL   Lab Invest 99 (11), 1714-1727 (2019)
   PUBMED   30683901
REFERENCE   3  (bases 1 to 854)
  AUTHORS   Limi,S., Zhao,Y., Guo,P., Lopez-Jones,M., Zheng,D., Singer,R.H.,
            Skoultchi,A.I. and Cvekl,A.
  TITLE     Bidirectional Analysis of Cryba4-Crybb1 Nascent Transcription and
            Nuclear Accumulation of Crybb3 mRNAs in Lens Fibers
  JOURNAL   Invest Ophthalmol Vis Sci 60 (1), 234-244 (2019)
   PUBMED   30646012
  REMARK    GeneRIF: The current study shows for the first time that two highly
            expressed lens crystallin genes, Cryba4 and Crybb1, can be
            simultaneously transcribed from adjacent bidirectional promoters
            and do not show nuclear accumulation.
REFERENCE   4  (bases 1 to 854)
  AUTHORS   Logan,C.M., Rajakaruna,S., Bowen,C., Radice,G.L., Robinson,M.L. and
            Menko,A.S.
  TITLE     N-cadherin regulates signaling mechanisms required for lens fiber
            cell elongation and lens morphogenesis
  JOURNAL   Dev Biol 428 (1), 118-134 (2017)
   PUBMED   28552735
REFERENCE   5  (bases 1 to 854)
  AUTHORS   Dickinson,M.E., Flenniken,A.M., Ji,X., Teboul,L., Wong,M.D.,
            White,J.K., Meehan,T.F., Weninger,W.J., Westerberg,H., Adissu,H.,
            Baker,C.N., Bower,L., Brown,J.M., Caddle,L.B., Chiani,F., Clary,D.,
            Cleak,J., Daly,M.J., Denegre,J.M., Doe,B., Dolan,M.E., Edie,S.M.,
            Fuchs,H., Gailus-Durner,V., Galli,A., Gambadoro,A., Gallegos,J.,
            Guo,S., Horner,N.R., Hsu,C.W., Johnson,S.J., Kalaga,S., Keith,L.C.,
            Lanoue,L., Lawson,T.N., Lek,M., Mark,M., Marschall,S., Mason,J.,
            McElwee,M.L., Newbigging,S., Nutter,L.M., Peterson,K.A.,
            Ramirez-Solis,R., Rowland,D.J., Ryder,E., Samocha,K.E.,
            Seavitt,J.R., Selloum,M., Szoke-Kovacs,Z., Tamura,M., Trainor,A.G.,
            Tudose,I., Wakana,S., Warren,J., Wendling,O., West,D.B., Wong,L.,
            Yoshiki,A., MacArthur,D.G., Tocchini-Valentini,G.P., Gao,X.,
            Flicek,P., Bradley,A., Skarnes,W.C., Justice,M.J., Parkinson,H.E.,
            Moore,M., Wells,S., Braun,R.E., Svenson,K.L., de Angelis,M.H.,
            Herault,Y., Mohun,T., Mallon,A.M., Henkelman,R.M., Brown,S.D.,
            Adams,D.J., Lloyd,K.C., McKerlie,C., Beaudet,A.L., Bucan,M. and
            Murray,S.A.
  CONSRTM   International Mouse Phenotyping Consortium; Jackson Laboratory;
            Infrastructure Nationale PHENOMIN, Institut Clinique de la Souris
            (ICS); Charles River Laboratories; MRC Harwell; Toronto Centre for
            Phenogenomics; Wellcome Trust Sanger Institute; RIKEN BioResource
            Center
  TITLE     High-throughput discovery of novel developmental phenotypes
  JOURNAL   Nature 537 (7621), 508-514 (2016)
   PUBMED   27626380
  REMARK    Erratum:[Nature. 2017 Nov 16;551(7680):398. doi:
            10.1038/nature24643.. PMID: 29144450]
REFERENCE   6  (bases 1 to 854)
  AUTHORS   Gonzalez,M., Ruggiero,F.P., Chang,Q., Shi,Y.J., Rich,M.M.,
            Kraner,S. and Balice-Gordon,R.J.
  TITLE     Disruption of Trkb-mediated signaling induces disassembly of
            postsynaptic receptor clusters at neuromuscular junctions
  JOURNAL   Neuron 24 (3), 567-583 (1999)
   PUBMED   10595510
REFERENCE   7  (bases 1 to 854)
  AUTHORS   Lovicu,F.J. and Overbeek,P.A.
  TITLE     Overlapping effects of different members of the FGF family on lens
            fiber differentiation in transgenic mice
  JOURNAL   Development 125 (17), 3365-3377 (1998)
   PUBMED   9693140
REFERENCE   8  (bases 1 to 854)
  AUTHORS   Reneker,L.W. and Overbeek,P.A.
  TITLE     Lens-specific expression of PDGF-A alters lens growth and
            development
  JOURNAL   Dev Biol 180 (2), 554-565 (1996)
   PUBMED   8954727
REFERENCE   9  (bases 1 to 854)
  AUTHORS   Hulsebos,T.J., Gilbert,D.J., Delattre,O., Smink,L.J., Dunham,I.,
            Westerveld,A., Thomas,G., Jenkins,N.A. and Copeland,N.G.
  TITLE     Assignment of the beta B1 crystallin gene (CRYBB1) to human
            chromosome 22 and mouse chromosome 5
  JOURNAL   Genomics 29 (3), 712-718 (1995)
   PUBMED   8575764
REFERENCE   10 (bases 1 to 854)
  AUTHORS   Hulsebos,T.J., Jenkins,N.A., Gilbert,D.J. and Copeland,N.G.
  TITLE     The beta crystallin genes on human chromosome 22 define a new
            region of homology with mouse chromosome 5
  JOURNAL   Genomics 25 (2), 574-576 (1995)
   PUBMED   7789995
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CJ046471.1, AJ272229.2 and BC031442.1.
            
            On or before Aug 8, 2019 this sequence version replaced
            NM_001359009.1, NM_021352.2.
            
            Transcript Variant: This variant (1) differs in the 5' UTR compared
            to variant 3. All eight variants encode the same protein.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: CO046330.1, CO046193.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-18                CJ046471.1         2-19
            19-826              AJ272229.2         3-810
            827-854             BC031442.1         818-845
FEATURES             Location/Qualifiers
     source          1..854
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="5"
                     /map="5 55.38 cM"
     gene            1..854
                     /gene="Crybb3"
                     /note="crystallin, beta B3"
                     /db_xref="GeneID:12962"
                     /db_xref="MGI:MGI:102717"
     exon            1..29
                     /gene="Crybb3"
                     /inference="alignment:Splign:2.1.0"
     exon            30..200
                     /gene="Crybb3"
                     /inference="alignment:Splign:2.1.0"
     CDS             126..761
                     /gene="Crybb3"
                     /note="beta-B3 crystallin"
                     /codon_start=1
                     /product="beta-crystallin B3"
                     /protein_id="NP_067327.1"
                     /db_xref="CCDS:CCDS19547.1"
                     /db_xref="GeneID:12962"
                     /db_xref="MGI:MGI:102717"
                     /translation="MAEQHGAPEQAAAGKSHGGLGGSYKVTVYELENFQGKRCELSAE
                     CPNLTDSLLEKVGSIQVESGPWLAFERRAFRGEQFVLEKGDYPRWDAWSSSRRSDILL
                     SLRPLHIDGPDHKLHLFENPAFSGRKMEIVDDDVPSLWAHGFQDRVASIRVINGTWVG
                     YEFPGYRGRQYVFERGEFRHWNEWDANQPQLQSVRRIRDQKWHKRGCFLSS"
     exon            201..319
                     /gene="Crybb3"
                     /inference="alignment:Splign:2.1.0"
     exon            320..452
                     /gene="Crybb3"
                     /inference="alignment:Splign:2.1.0"
     exon            453..595
                     /gene="Crybb3"
                     /inference="alignment:Splign:2.1.0"
     exon            596..828
                     /gene="Crybb3"
                     /inference="alignment:Splign:2.1.0"
     regulatory      807..812
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Crybb3"
                     /note="hexamer: AATAAA"
     polyA_site      828
                     /gene="Crybb3"
                     /note="major polyA site"
ORIGIN      
        1 agtctggaga caactggtcc tggaagcagt gctgtttggg tgggaaccca ccagtccctg
       61 atcctgctgg ccgcagctgg tgacctggtt ctcctccagt ttgaggcaga agtatcccca
      121 gagagatggc ggagcagcac ggagcacctg agcaggctgc agctggcaag agccatggag
      181 gccttggggg cagctacaag gtgacggtgt acgagttaga gaacttccag ggcaagcgat
      241 gcgagctctc ggcggagtgt cccaacctca ccgacagcct gttggagaag gtgggctcca
      301 tccaagtgga gtctggaccg tggctggcgt tcgagcgcag ggcctttcgt ggggagcagt
      361 ttgttctgga gaagggggac taccctcgct gggatgcctg gtccagcagc cgacgtagtg
      421 acattctcct gtccctccgt cctttgcaca ttgatggccc agaccacaag ctgcacctgt
      481 ttgagaaccc agccttcagt gggcgcaaga tggagatcgt agatgacgat gtgcccagcc
      541 tgtgggccca tggtttccag gaccgtgtgg ccagcatccg tgtcatcaac gggacgtggg
      601 tgggctacga gttcccgggc taccgtgggc gccagtacgt gttcgagcgg ggcgaattcc
      661 gccactggaa cgagtgggac gccaaccagc cgcagctgca gtcggtgcgc cgcatccgcg
      721 accagaagtg gcacaagcgc ggctgcttcc tgagcagctg aggactcccg ggagccaacg
      781 cctgtgtccc cttcctgaac ctgctcaata aagcccacca gccacgcaaa aaaaaaaaaa
      841 aaaaaaaaaa aaaa
//
