LOCUS       NM_001359005             820 bp    mRNA    linear   ROD 11-FEB-2025
DEFINITION  Mus musculus crystallin, beta B3 (Crybb3), transcript variant 4,
            mRNA.
ACCESSION   NM_001359005
VERSION     NM_001359005.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 820)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 820)
  AUTHORS   Yu,W., Yu,Z., Wu,D., Zhang,J., Zhu,Y., Zhang,Y., Ning,H., Wang,M.,
            Zhang,J. and Zhao,J.
  TITLE     Lens-specific conditional knockout of Msx2 in mice leads to ocular
            anterior segment dysgenesis via activation of a calcium signaling
            pathway
  JOURNAL   Lab Invest 99 (11), 1714-1727 (2019)
   PUBMED   30683901
REFERENCE   3  (bases 1 to 820)
  AUTHORS   Limi,S., Zhao,Y., Guo,P., Lopez-Jones,M., Zheng,D., Singer,R.H.,
            Skoultchi,A.I. and Cvekl,A.
  TITLE     Bidirectional Analysis of Cryba4-Crybb1 Nascent Transcription and
            Nuclear Accumulation of Crybb3 mRNAs in Lens Fibers
  JOURNAL   Invest Ophthalmol Vis Sci 60 (1), 234-244 (2019)
   PUBMED   30646012
  REMARK    GeneRIF: The current study shows for the first time that two highly
            expressed lens crystallin genes, Cryba4 and Crybb1, can be
            simultaneously transcribed from adjacent bidirectional promoters
            and do not show nuclear accumulation.
REFERENCE   4  (bases 1 to 820)
  AUTHORS   Logan,C.M., Rajakaruna,S., Bowen,C., Radice,G.L., Robinson,M.L. and
            Menko,A.S.
  TITLE     N-cadherin regulates signaling mechanisms required for lens fiber
            cell elongation and lens morphogenesis
  JOURNAL   Dev Biol 428 (1), 118-134 (2017)
   PUBMED   28552735
REFERENCE   5  (bases 1 to 820)
  AUTHORS   Dickinson,M.E., Flenniken,A.M., Ji,X., Teboul,L., Wong,M.D.,
            White,J.K., Meehan,T.F., Weninger,W.J., Westerberg,H., Adissu,H.,
            Baker,C.N., Bower,L., Brown,J.M., Caddle,L.B., Chiani,F., Clary,D.,
            Cleak,J., Daly,M.J., Denegre,J.M., Doe,B., Dolan,M.E., Edie,S.M.,
            Fuchs,H., Gailus-Durner,V., Galli,A., Gambadoro,A., Gallegos,J.,
            Guo,S., Horner,N.R., Hsu,C.W., Johnson,S.J., Kalaga,S., Keith,L.C.,
            Lanoue,L., Lawson,T.N., Lek,M., Mark,M., Marschall,S., Mason,J.,
            McElwee,M.L., Newbigging,S., Nutter,L.M., Peterson,K.A.,
            Ramirez-Solis,R., Rowland,D.J., Ryder,E., Samocha,K.E.,
            Seavitt,J.R., Selloum,M., Szoke-Kovacs,Z., Tamura,M., Trainor,A.G.,
            Tudose,I., Wakana,S., Warren,J., Wendling,O., West,D.B., Wong,L.,
            Yoshiki,A., MacArthur,D.G., Tocchini-Valentini,G.P., Gao,X.,
            Flicek,P., Bradley,A., Skarnes,W.C., Justice,M.J., Parkinson,H.E.,
            Moore,M., Wells,S., Braun,R.E., Svenson,K.L., de Angelis,M.H.,
            Herault,Y., Mohun,T., Mallon,A.M., Henkelman,R.M., Brown,S.D.,
            Adams,D.J., Lloyd,K.C., McKerlie,C., Beaudet,A.L., Bucan,M. and
            Murray,S.A.
  CONSRTM   International Mouse Phenotyping Consortium; Jackson Laboratory;
            Infrastructure Nationale PHENOMIN, Institut Clinique de la Souris
            (ICS); Charles River Laboratories; MRC Harwell; Toronto Centre for
            Phenogenomics; Wellcome Trust Sanger Institute; RIKEN BioResource
            Center
  TITLE     High-throughput discovery of novel developmental phenotypes
  JOURNAL   Nature 537 (7621), 508-514 (2016)
   PUBMED   27626380
  REMARK    Erratum:[Nature. 2017 Nov 16;551(7680):398. doi:
            10.1038/nature24643.. PMID: 29144450]
REFERENCE   6  (bases 1 to 820)
  AUTHORS   Gonzalez,M., Ruggiero,F.P., Chang,Q., Shi,Y.J., Rich,M.M.,
            Kraner,S. and Balice-Gordon,R.J.
  TITLE     Disruption of Trkb-mediated signaling induces disassembly of
            postsynaptic receptor clusters at neuromuscular junctions
  JOURNAL   Neuron 24 (3), 567-583 (1999)
   PUBMED   10595510
REFERENCE   7  (bases 1 to 820)
  AUTHORS   Lovicu,F.J. and Overbeek,P.A.
  TITLE     Overlapping effects of different members of the FGF family on lens
            fiber differentiation in transgenic mice
  JOURNAL   Development 125 (17), 3365-3377 (1998)
   PUBMED   9693140
REFERENCE   8  (bases 1 to 820)
  AUTHORS   Reneker,L.W. and Overbeek,P.A.
  TITLE     Lens-specific expression of PDGF-A alters lens growth and
            development
  JOURNAL   Dev Biol 180 (2), 554-565 (1996)
   PUBMED   8954727
REFERENCE   9  (bases 1 to 820)
  AUTHORS   Hulsebos,T.J., Gilbert,D.J., Delattre,O., Smink,L.J., Dunham,I.,
            Westerveld,A., Thomas,G., Jenkins,N.A. and Copeland,N.G.
  TITLE     Assignment of the beta B1 crystallin gene (CRYBB1) to human
            chromosome 22 and mouse chromosome 5
  JOURNAL   Genomics 29 (3), 712-718 (1995)
   PUBMED   8575764
REFERENCE   10 (bases 1 to 820)
  AUTHORS   Hulsebos,T.J., Jenkins,N.A., Gilbert,D.J. and Copeland,N.G.
  TITLE     The beta crystallin genes on human chromosome 22 define a new
            region of homology with mouse chromosome 5
  JOURNAL   Genomics 25 (2), 574-576 (1995)
   PUBMED   7789995
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC162528.5 and CF739665.1.
            
            Transcript Variant: This variant (4) differs in the 5' UTR compared
            to variant 3. All eight variants encode the same protein.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: CO046309.1, CF739665.1 [ECO:0000332]
            RNAseq introns              :: partial sample support SAMN00849383,
                                           SAMN01164136 [ECO:0000350]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-14                AC162528.5         65312-65325         c
            15-642              CF739665.1         1-628
            643-820             AC162528.5         59580-59757         c
FEATURES             Location/Qualifiers
     source          1..820
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="5"
                     /map="5 55.38 cM"
     gene            1..820
                     /gene="Crybb3"
                     /note="crystallin, beta B3"
                     /db_xref="GeneID:12962"
                     /db_xref="MGI:MGI:102717"
     exon            1..29
                     /gene="Crybb3"
                     /inference="alignment:Splign:2.1.0"
     exon            30..91
                     /gene="Crybb3"
                     /inference="alignment:Splign:2.1.0"
     exon            92..192
                     /gene="Crybb3"
                     /inference="alignment:Splign:2.1.0"
     CDS             118..753
                     /gene="Crybb3"
                     /note="beta-B3 crystallin"
                     /codon_start=1
                     /product="beta-crystallin B3"
                     /protein_id="NP_001345934.1"
                     /db_xref="CCDS:CCDS19547.1"
                     /db_xref="GeneID:12962"
                     /db_xref="MGI:MGI:102717"
                     /translation="MAEQHGAPEQAAAGKSHGGLGGSYKVTVYELENFQGKRCELSAE
                     CPNLTDSLLEKVGSIQVESGPWLAFERRAFRGEQFVLEKGDYPRWDAWSSSRRSDILL
                     SLRPLHIDGPDHKLHLFENPAFSGRKMEIVDDDVPSLWAHGFQDRVASIRVINGTWVG
                     YEFPGYRGRQYVFERGEFRHWNEWDANQPQLQSVRRIRDQKWHKRGCFLSS"
     exon            193..311
                     /gene="Crybb3"
                     /inference="alignment:Splign:2.1.0"
     exon            312..444
                     /gene="Crybb3"
                     /inference="alignment:Splign:2.1.0"
     exon            445..587
                     /gene="Crybb3"
                     /inference="alignment:Splign:2.1.0"
     exon            588..820
                     /gene="Crybb3"
                     /inference="alignment:Splign:2.1.0"
     regulatory      799..804
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Crybb3"
                     /note="hexamer: AATAAA"
     polyA_site      820
                     /gene="Crybb3"
                     /note="major polyA site"
ORIGIN      
        1 agtctggaga caactggtcc tggaagcagg tctctgcctc ctgaggctaa gagagacttc
       61 cagggtccac tatgcgagag cctagaacac gtttgaggca gaagtatccc cagagagatg
      121 gcggagcagc acggagcacc tgagcaggct gcagctggca agagccatgg aggccttggg
      181 ggcagctaca aggtgacggt gtacgagtta gagaacttcc agggcaagcg atgcgagctc
      241 tcggcggagt gtcccaacct caccgacagc ctgttggaga aggtgggctc catccaagtg
      301 gagtctggac cgtggctggc gttcgagcgc agggcctttc gtggggagca gtttgttctg
      361 gagaaggggg actaccctcg ctgggatgcc tggtccagca gccgacgtag tgacattctc
      421 ctgtccctcc gtcctttgca cattgatggc ccagaccaca agctgcacct gtttgagaac
      481 ccagccttca gtgggcgcaa gatggagatc gtagatgacg atgtgcccag cctgtgggcc
      541 catggtttcc aggaccgtgt ggccagcatc cgtgtcatca acgggacgtg ggtgggctac
      601 gagttcccgg gctaccgtgg gcgccagtac gtgttcgagc ggggcgaatt ccgccactgg
      661 aacgagtggg acgccaacca gccgcagctg cagtcggtgc gccgcatccg cgaccagaag
      721 tggcacaagc gcggctgctt cctgagcagc tgaggactcc cgggagccaa cgcctgtgtc
      781 cccttcctga acctgctcaa taaagcccac cagccacgca
//
