LOCUS       NM_021351                819 bp    mRNA    linear   ROD 07-FEB-2025
DEFINITION  Mus musculus crystallin, beta A4 (Cryba4), transcript variant 1,
            mRNA.
ACCESSION   NM_021351
VERSION     NM_021351.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 819)
  AUTHORS   Martynova,E., Zhao,Y., Xie,Q., Zheng,D. and Cvekl,A.
  TITLE     Transcriptomic analysis and novel insights into lens fibre cell
            differentiation regulated by Gata3
  JOURNAL   Open Biol 9 (12), 190220 (2019)
   PUBMED   31847788
REFERENCE   2  (bases 1 to 819)
  AUTHORS   Limi,S., Zhao,Y., Guo,P., Lopez-Jones,M., Zheng,D., Singer,R.H.,
            Skoultchi,A.I. and Cvekl,A.
  TITLE     Bidirectional Analysis of Cryba4-Crybb1 Nascent Transcription and
            Nuclear Accumulation of Crybb3 mRNAs in Lens Fibers
  JOURNAL   Invest Ophthalmol Vis Sci 60 (1), 234-244 (2019)
   PUBMED   30646012
  REMARK    GeneRIF: The current study shows for the first time that two highly
            expressed lens crystallin genes, Cryba4 and Crybb1, can be
            simultaneously transcribed from adjacent bidirectional promoters
            and do not show nuclear accumulation.
REFERENCE   3  (bases 1 to 819)
  AUTHORS   Hayashi,N., Sato,T., Kokabu,S., Usui,M., Yumoto,M., Ikami,E.,
            Sakamoto,Y., Nifuji,A., Hayata,T., Noda,M. and Yoda,T.
  TITLE     Possible association of oestrogen and Cryba4 with masticatory
            muscle tendon-aponeurosis hyperplasia
  JOURNAL   Oral Dis 25 (1), 274-281 (2019)
   PUBMED   29683234
  REMARK    GeneRIF: Oestrogen and Cryba4 may be associated with the
            progression of masticatory muscle tendon-aponeurosis hyperplasia.
            Erratum:[Oral Dis. 2023 Mar;29(2):854. doi: 10.1111/odi.13956..
            PMID: 34450689]
REFERENCE   4  (bases 1 to 819)
  AUTHORS   Xie,Q. and Cvekl,A.
  TITLE     The orchestration of mammalian tissue morphogenesis through a
            series of coherent feed-forward loops
  JOURNAL   J Biol Chem 286 (50), 43259-43271 (2011)
   PUBMED   21998302
REFERENCE   5  (bases 1 to 819)
  AUTHORS   Pirity,M.K., Wang,W.L., Wolf,L.V., Tamm,E.R., Schreiber-Agus,N. and
            Cvekl,A.
  TITLE     Rybp, a polycomb complex-associated protein, is required for mouse
            eye development
  JOURNAL   BMC Dev Biol 7, 39 (2007)
   PUBMED   17470285
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 819)
  AUTHORS   Gonzalez,M., Ruggiero,F.P., Chang,Q., Shi,Y.J., Rich,M.M.,
            Kraner,S. and Balice-Gordon,R.J.
  TITLE     Disruption of Trkb-mediated signaling induces disassembly of
            postsynaptic receptor clusters at neuromuscular junctions
  JOURNAL   Neuron 24 (3), 567-583 (1999)
   PUBMED   10595510
REFERENCE   7  (bases 1 to 819)
  AUTHORS   Lovicu,F.J. and Overbeek,P.A.
  TITLE     Overlapping effects of different members of the FGF family on lens
            fiber differentiation in transgenic mice
  JOURNAL   Development 125 (17), 3365-3377 (1998)
   PUBMED   9693140
REFERENCE   8  (bases 1 to 819)
  AUTHORS   Bertuzzi,S., Sheng,H.Z., Copeland,N.G., Gilbert,D.J., Jenkins,N.A.,
            Taira,M., Dawid,I.B. and Westphal,H.
  TITLE     Molecular cloning, structure, and chromosomal localization of the
            mouse LIM/homeobox gene Lhx5
  JOURNAL   Genomics 36 (2), 234-239 (1996)
   PUBMED   8812449
REFERENCE   9  (bases 1 to 819)
  AUTHORS   Hulsebos,T.J., Gilbert,D.J., Delattre,O., Smink,L.J., Dunham,I.,
            Westerveld,A., Thomas,G., Jenkins,N.A. and Copeland,N.G.
  TITLE     Assignment of the beta B1 crystallin gene (CRYBB1) to human
            chromosome 22 and mouse chromosome 5
  JOURNAL   Genomics 29 (3), 712-718 (1995)
   PUBMED   8575764
REFERENCE   10 (bases 1 to 819)
  AUTHORS   Hulsebos,T.J., Jenkins,N.A., Gilbert,D.J. and Copeland,N.G.
  TITLE     The beta crystallin genes on human chromosome 22 define a new
            region of homology with mouse chromosome 5
  JOURNAL   Genomics 25 (2), 574-576 (1995)
   PUBMED   7789995
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AK132591.1, AJ272228.2 and
            BC056444.1.
            
            [WARNING] On Mar 12, 2025 this sequence was replaced by
            NM_021351.3.
            
            On Aug 20, 2015 this sequence version replaced NM_021351.1.
            
            Summary: This gene encodes a member of the crystallin family of
            proteins that contribute to the transparency and refractive
            properties of the ocular lens. Certain mutations in the human
            ortholog of this gene are associated with cataract and bilateral
            microphthalmia. This gene is located adjacent to a related
            crystallin gene on chromosome 5. Alternative splicing results in
            multiple transcript variants encoding different isoforms. [provided
            by RefSeq, Aug 2015].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer protein (isoform 1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC058703.1, AK132591.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849381 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-16                AK132591.1         2-17
            17-768              AJ272228.2         1-752
            769-819             BC056444.1         743-793
FEATURES             Location/Qualifiers
     source          1..819
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="5"
                     /map="5 54.62 cM"
     gene            1..819
                     /gene="Cryba4"
                     /note="crystallin, beta A4"
                     /db_xref="GeneID:12959"
                     /db_xref="MGI:MGI:102716"
     exon            1..27
                     /gene="Cryba4"
                     /inference="alignment:Splign:2.1.0"
     exon            28..78
                     /gene="Cryba4"
                     /inference="alignment:Splign:2.1.0"
     CDS             40..630
                     /gene="Cryba4"
                     /note="isoform 1 is encoded by transcript variant 1;
                     beta-crystallin A4; beta-A4 crystallin"
                     /codon_start=1
                     /product="beta-crystallin A4 isoform 1"
                     /protein_id="NP_067326.1"
                     /db_xref="CCDS:CCDS19536.1"
                     /db_xref="GeneID:12959"
                     /db_xref="MGI:MGI:102716"
                     /translation="MTLQCTKSAGHWRMVVWDEEGFQGRRHEFTAECPSVLELGFETV
                     RSLKVLSGAWVGFEHAGFQGQQYVLERGDYPGWDAWGGNTAYPAERLTSFRPVACANH
                     RDSRLTIFEQENFLGRKGELNDDYPSLQAMGWDGTEVGSFHVQSGAWVCSQFPGYRGF
                     QYILESDHHSGDYKHFREWGSHAHTFQVQSVRRIQQ"
     exon            79..197
                     /gene="Cryba4"
                     /inference="alignment:Splign:2.1.0"
     exon            198..339
                     /gene="Cryba4"
                     /inference="alignment:Splign:2.1.0"
     exon            340..482
                     /gene="Cryba4"
                     /inference="alignment:Splign:2.1.0"
     exon            483..809
                     /gene="Cryba4"
                     /inference="alignment:Splign:2.1.0"
     regulatory      782..787
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Cryba4"
                     /note="hexamer: AATAAA"
     polyA_site      788
                     /gene="Cryba4"
                     /note="major polyA site"
     polyA_site      809
                     /gene="Cryba4"
ORIGIN      
        1 tctgacgtgt gctctgggcc cttcttggac cgtgccaaca tgaccctgca gtgcaccaag
       61 tcagctggac actggaggat ggtggtgtgg gatgaagaag gcttccaggg ccgacggcat
      121 gaattcacag ctgagtgtcc cagtgtcctg gaacttggtt ttgagacggt gcgatctctc
      181 aaagtcctga gcggagcgtg ggtaggcttt gagcacgccg gcttccaagg acagcaatat
      241 gtgctggaga ggggcgatta cccgggctgg gatgcctggg gtggcaacac agcctaccct
      301 gcggagaggc tcacctcctt ccggcctgtg gcctgcgcta accaccgcga ctcaaggctg
      361 accatcttcg agcaggagaa cttcctgggc aggaaaggcg agctgaacga tgactatccc
      421 tctctgcagg ccatgggctg ggacggcact gaagtgggct ccttccatgt tcaatctggg
      481 gcgtgggttt gttcccagtt tcctggctac cgaggttttc agtacatcct ggagagcgat
      541 caccactcag gtgactacaa gcacttcaga gagtggggct cccatgctca caccttccag
      601 gtgcagagtg tgcgcagaat ccagcagtga gcacgcactg gcgacctggg gtgcctgcga
      661 aacgactggg tgactggccg gaggatgtgg ctgctcttgg ttctggctac ccttgtgtcc
      721 tctgggaacc ccacatccct gtcagccagc ccatgccccg ccagctcatg gagctcaaag
      781 aaataaaatg aaaaacaaaa gactcagtaa aaaaaaaaa
//
