LOCUS       NM_009964                975 bp    mRNA    linear   ROD 10-FEB-2025
DEFINITION  Mus musculus crystallin, alpha B (Cryab), transcript variant 2,
            mRNA.
ACCESSION   NM_009964
VERSION     NM_009964.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 975)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 975)
  AUTHORS   Hagen,K.M., Gordon,P., Frederick,A., Palmer,A.L., Edalat,P.,
            Zonta,Y.R., Scott,L., Flancia,M., Reid,J.K., Joel,M. and
            Ousman,S.S.
  TITLE     CRYAB plays a role in terminating the presence of pro-inflammatory
            macrophages in the older, injured mouse peripheral nervous system
  JOURNAL   Neurobiol Aging 133, 1-15 (2024)
   PUBMED   38381471
  REMARK    GeneRIF: CRYAB plays a role in terminating the presence of
            pro-inflammatory macrophages in the older, injured mouse peripheral
            nervous system.
REFERENCE   3  (bases 1 to 975)
  AUTHORS   Chou,C., Martin,G.L., Perera,G., Awata,J., Larson,A., Blanton,R.
            and Chin,M.T.
  TITLE     A novel alphaB-crystallin R123W variant drives hypertrophic
            cardiomyopathy by promoting maladaptive calcium-dependent signal
            transduction
  JOURNAL   Front Cardiovasc Med 10, 1223244 (2023)
   PUBMED   37435054
  REMARK    Erratum:[Front Cardiovasc Med. 2024 Aug 16;11:1455263. doi:
            10.3389/fcvm.2024.1455263.. PMID: 39221424]
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 975)
  AUTHORS   Thomas,O.G., Bronge,M., Tengvall,K., Akpinar,B., Nilsson,O.B.,
            Holmgren,E., Hessa,T., Gafvelin,G., Khademi,M., Alfredsson,L.,
            Martin,R., Guerreiro-Cacais,A.O., Gronlund,H., Olsson,T. and
            Kockum,I.
  TITLE     Cross-reactive EBNA1 immunity targets alpha-crystallin B and is
            associated with multiple sclerosis
  JOURNAL   Sci Adv 9 (20), eadg3032 (2023)
   PUBMED   37196088
  REMARK    GeneRIF: Cross-reactive EBNA1 immunity targets alpha-crystallin B
            and is associated with multiple sclerosis.
REFERENCE   5  (bases 1 to 975)
  AUTHORS   Frederikse,P.H., Dubin,R.A., Haynes,J.I. 2nd and Piatigorsky,J.
  TITLE     Structure and alternate tissue-preferred transcription initiation
            of the mouse alpha B-crystallin/small heat shock protein gene
  JOURNAL   Nucleic Acids Res 22 (25), 5686-5694 (1994)
   PUBMED   7838723
REFERENCE   6  (bases 1 to 975)
  AUTHORS   Dubin,R.A., Gopal-Srivastava,R., Wawrousek,E.F. and Piatigorsky,J.
  TITLE     Expression of the murine alpha B-crystallin gene in lens and
            skeletal muscle: identification of a muscle-preferred enhancer
  JOURNAL   Mol Cell Biol 11 (9), 4340-4349 (1991)
   PUBMED   1875925
REFERENCE   7  (bases 1 to 975)
  AUTHORS   Klemenz,R., Frohli,E., Aoyama,A., Hoffmann,S., Simpson,R.J.,
            Moritz,R.L. and Schafer,R.
  TITLE     Alpha B crystallin accumulation is a specific response to Ha-ras
            and v-mos oncogene expression in mouse NIH 3T3 fibroblasts
  JOURNAL   Mol Cell Biol 11 (2), 803-812 (1991)
   PUBMED   1846673
REFERENCE   8  (bases 1 to 975)
  AUTHORS   Iwaki,T., Kume-Iwaki,A., Liem,R.K. and Goldman,J.E.
  TITLE     Alpha B-crystallin is expressed in non-lenticular tissues and
            accumulates in Alexander's disease brain
  JOURNAL   Cell 57 (1), 71-78 (1989)
   PUBMED   2539261
REFERENCE   9  (bases 1 to 975)
  AUTHORS   Dubin,R.A., Wawrousek,E.F. and Piatigorsky,J.
  TITLE     Expression of the murine alpha B-crystallin gene is not restricted
            to the lens
  JOURNAL   Mol Cell Biol 9 (3), 1083-1091 (1989)
   PUBMED   2725488
REFERENCE   10 (bases 1 to 975)
  AUTHORS   Quax-Jeuken,Y., Quax,W., van Rens,G., Khan,P.M. and Bloemendal,H.
  TITLE     Complete structure of the alpha B-crystallin gene: conservation of
            the exon-intron distribution in the two nonlinked alpha-crystallin
            genes
  JOURNAL   Proc Natl Acad Sci U S A 82 (17), 5819-5823 (1985)
   PUBMED   3862098
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC123614.6 and AK168999.1.
            
            On Jan 31, 2014 this sequence version replaced NM_009964.2.
            
            Summary: This gene encodes a member of the small heat-shock protein
            (HSP20) family. The encoded protein is a molecular chaperone that
            protects proteins against thermal denaturation and other stresses.
            This protein is a component of the eye lens, regulates lens
            differentiation and functions as a refractive element in the lens.
            This protein is a negative regulator of inflammation, has
            anti-apoptotic properties and also plays a role in the formation of
            muscular tissue. Mice lacking this gene exhibit worse experimental
            autoimmune encephalomyelitis and inflammation of the central
            nervous system compared to the wild type. In mouse models, this
            gene has a critical role in alleviating the pathology of the
            neurodegenerative Alexander disease. Mutations in the human gene
            are associated with myofibrillar myopathy 2, fatal infantile
            hypertonic myofibrillar myopathy, multiple types of cataract and
            dilated cardiomyopathy. Alternative splicing results in multiple
            transcript variants. [provided by RefSeq, Jan 2014].
            
            Transcript Variant: This variant (2) differs in the 5' UTR compared
            to variant 1. Variants 1 through 4 encode the same protein.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: CK128036.1, CN539070.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-262               AC123614.6         98486-98747
            263-888             AK168999.1         2-627
            889-889             AC123614.6         102065-102065
            890-969             AK168999.1         629-708
            970-975             AC123614.6         102146-102151
FEATURES             Location/Qualifiers
     source          1..975
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="9"
                     /map="9 27.75 cM"
     gene            1..975
                     /gene="Cryab"
                     /gene_synonym="Crya-2; Crya2; HspB5; P23"
                     /note="crystallin, alpha B"
                     /db_xref="GeneID:12955"
                     /db_xref="MGI:MGI:88516"
     exon            1..507
                     /gene="Cryab"
                     /gene_synonym="Crya-2; Crya2; HspB5; P23"
                     /inference="alignment:Splign:2.1.0"
     CDS             307..834
                     /gene="Cryab"
                     /gene_synonym="Crya-2; Crya2; HspB5; P23"
                     /note="alpha(B)-crystallin; crystallin, alpha 2; heat
                     shock protein CryAB; heat shock protein HspB5"
                     /codon_start=1
                     /product="alpha-crystallin B chain"
                     /protein_id="NP_034094.1"
                     /db_xref="CCDS:CCDS23172.1"
                     /db_xref="GeneID:12955"
                     /db_xref="MGI:MGI:88516"
                     /translation="MDIAIHHPWIRRPFFPFHSPSRLFDQFFGEHLLESDLFSTATSL
                     SPFYLRPPSFLRAPSWIDTGLSEMRLEKDRFSVNLDVKHFSPEELKVKVLGDVIEVHG
                     KHEERQDEHGFISREFHRKYRIPADVDPLTITSSLSSDGVLTVNGPRKQVSGPERTIP
                     ITREEKPAVAAAPKK"
     exon            508..630
                     /gene="Cryab"
                     /gene_synonym="Crya-2; Crya2; HspB5; P23"
                     /inference="alignment:Splign:2.1.0"
     exon            631..975
                     /gene="Cryab"
                     /gene_synonym="Crya-2; Crya2; HspB5; P23"
                     /inference="alignment:Splign:2.1.0"
     regulatory      951..956
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Cryab"
                     /gene_synonym="Crya-2; Crya2; HspB5; P23"
                     /note="hexamer: AATAAA"
     polyA_site      975
                     /gene="Cryab"
                     /gene_synonym="Crya-2; Crya2; HspB5; P23"
                     /note="major polyA site"
ORIGIN      
        1 agagaacaag gatggggtgg gtgccactgg gtgtggacag agagctagtg aaacaagacc
       61 atgacaagtc accggtcagc tcagccctgc ctgtgtttct cttttcttag ctcagtgagt
      121 accgggtatg tgtcaccctg ccaaatccct gatcacaagt ctccatgaac tggcggtgag
      181 ctgggataat aaaacccctg acctcaccat tccagaagct tcagaagact gcatatataa
      241 ggggccggct ggagctgctg ctgaaggagt tgaccagcca accgactctg cattcatcta
      301 gccacaatgg acatcgccat ccaccacccc tggatccggc gccccttctt ccccttccac
      361 tccccaagcc gcctcttcga ccagttcttc ggagagcacc tgttggagtc tgacctcttc
      421 tcaacagcca cttccctgag ccccttctac cttcggccac cctccttcct gcgggcaccc
      481 agctggattg acaccggact ctcagagatg cgtttggaga aggacagatt ctctgtgaat
      541 ctggacgtga agcacttctc tccggaggaa ctcaaagtca aggttctggg ggacgtgatt
      601 gaggtccacg gcaagcacga agaacgccag gacgaacatg gcttcatctc cagggagttc
      661 cacaggaagt accggatccc agccgatgtg gatcctctca ccatcacttc atccctgtca
      721 tctgatggag tcctcactgt gaatggacca aggaaacagg tgtctggccc tgagcgcacc
      781 attcccatca cccgtgaaga gaagcctgct gtcgccgcag cccctaagaa gtagatcccc
      841 tttcctcatt gagttttttt taaaacaagg aagtttccca tcagtgattg aaaatctgtg
      901 actagtgctg aagcttatta atgctaaggg ctggcccaga ttattaagct aataaaaata
      961 tcattcagca acaaa
//
