LOCUS       NM_001278570            1127 bp    mRNA    linear   ROD 12-JUN-2024
DEFINITION  Mus musculus crystallin, alpha A (Cryaa), transcript variant 3,
            mRNA.
ACCESSION   NM_001278570
VERSION     NM_001278570.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1127)
  AUTHORS   Wei,Z., Hao,C., Chen,J.K., Gan,L. and Fan,X.
  TITLE     A tamoxifen-inducible Cre knock-in mouse for lens-specific gene
            manipulation
  JOURNAL   Exp Eye Res 226, 109306 (2023)
   PUBMED   36372215
REFERENCE   2  (bases 1 to 1127)
  AUTHORS   Jia,Z.K., Fu,C.X., Wang,A.L., Yao,K. and Chen,X.J.
  TITLE     Cataract-causing allele in CRYAA (Y118D) proceeds through
            endoplasmic reticulum stress in mouse model
  JOURNAL   Zool Res 42 (3), 300-309 (2021)
   PUBMED   33929105
  REMARK    GeneRIF: Cataract-causing allele in CRYAA (Y118D) proceeds through
            endoplasmic reticulum stress in mouse model.
REFERENCE   3  (bases 1 to 1127)
  AUTHORS   Chang,B., Hawes,N.L., Roderick,T.H., Smith,R.S., Heckenlively,J.R.,
            Horwitz,J. and Davisson,M.T.
  TITLE     Identification of a missense mutation in the alphaA-crystallin gene
            of the lop18 mouse
  JOURNAL   Mol Vis 5, 21 (1999)
   PUBMED   10493778
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 1127)
  AUTHORS   Piatigorsky,J.
  TITLE     Gene sharing in lens and cornea: facts and implications
  JOURNAL   Prog Retin Eye Res 17 (2), 145-174 (1998)
   PUBMED   9695791
  REMARK    GeneRIF: Alpha A crystallin is a moonlighting protein that
            functions as a heat shock protein as well as a lens crystalline.
            Review article
            Erratum:[Prog Retin Eye Res 1999 Jul;18(4):552]
REFERENCE   5  (bases 1 to 1127)
  AUTHORS   Brady,J.P., Garland,D., Duglas-Tabor,Y., Robison,W.G. Jr.,
            Groome,A. and Wawrousek,E.F.
  TITLE     Targeted disruption of the mouse alpha A-crystallin gene induces
            cataract and cytoplasmic inclusion bodies containing the small heat
            shock protein alpha B-crystallin
  JOURNAL   Proc Natl Acad Sci U S A 94 (3), 884-889 (1997)
   PUBMED   9023351
REFERENCE   6  (bases 1 to 1127)
  AUTHORS   Sertic,J., Zaleska-Rutczynska,Z., Vincek,V., Nadeau,J.H.,
            Figueroa,F. and Klein,J.
  TITLE     Mapping of six DNA markers on mouse chromosome 17
  JOURNAL   Mamm Genome 2 (2), 138-142 (1992)
   PUBMED   1347473
REFERENCE   7  (bases 1 to 1127)
  AUTHORS   Kaye,N.W., Lalley,P.A., Petrash,J.M. and Church,R.L.
  TITLE     Regional assignment of the mouse alpha A2-crystallin gene (Crya-1)
            to chromosome 17A3----B by in situ hybridization
  JOURNAL   Cytogenet Cell Genet 53 (2-3), 95-96 (1990)
   PUBMED   2369847
REFERENCE   8  (bases 1 to 1127)
  AUTHORS   Chepelinsky,A.B., Sommer,B. and Piatigorsky,J.
  TITLE     Interaction between two different regulatory elements activates the
            murine alpha A-crystallin gene promoter in explanted lens epithelia
  JOURNAL   Mol Cell Biol 7 (5), 1807-1814 (1987)
   PUBMED   3474517
REFERENCE   9  (bases 1 to 1127)
  AUTHORS   Skow,L.C. and Donner,M.E.
  TITLE     The locus encoding alpha A-crystallin is closely linked to H-2K on
            mouse chromosome 17
  JOURNAL   Genetics 110 (4), 723-732 (1985)
   PUBMED   2993101
REFERENCE   10 (bases 1 to 1127)
  AUTHORS   King,C.R. and Piatigorsky,J.
  TITLE     Alternative RNA splicing of the murine alpha A-crystallin gene:
            protein-coding information within an intron
  JOURNAL   Cell 32 (3), 707-712 (1983)
   PUBMED   6187470
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from EL604937.1, AK143463.1 and
            BB371615.1.
            
            Summary: This gene encodes subunit a, one of two subunits of
            alpha-crystallin, which is a high molecular weight, soluble
            aggregate and is a member of the small heat shock protein (sHSP)
            family. The encoded protein has been identified as a moonlighting
            protein based on its ability to perform mechanistically distinct
            functions. It acts as a molecular chaperone and is the major
            protein in the eye lens, maintaining the transparency and
            refractive index of the lens. In mouse, deficiency in this gene is
            associated with smaller lenses and eyes and with increasing lens
            opacity with age. Alternative splicing results in multiple
            transcript variants. [provided by RefSeq, Jan 2014].
            
            Transcript Variant: This variant (3) lacks an alternate in-frame
            exon in the coding region compared to variant 1. It encodes isoform
            3 which is shorter compared to isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: CB844022.2, AK143463.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849383, SAMN00849385
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            multifunctional gene product(s) :: PMID: 9695791
            RefSeq Select criteria          :: based on conservation,
                                               expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2                 EL604937.1         1-2
            3-1119              AK143463.1         2-1118
            1120-1127           BB371615.1         283-290
FEATURES             Location/Qualifiers
     source          1..1127
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="17"
                     /map="17 17.09 cM"
     gene            1..1127
                     /gene="Cryaa"
                     /gene_synonym="Acry-1; Crya-1; Crya1; DAcry-1; lop18"
                     /note="crystallin, alpha A"
                     /db_xref="GeneID:12954"
                     /db_xref="MGI:MGI:88515"
     exon            1..259
                     /gene="Cryaa"
                     /gene_synonym="Acry-1; Crya-1; Crya1; DAcry-1; lop18"
                     /inference="alignment:Splign:2.1.0"
     exon            260..382
                     /gene="Cryaa"
                     /gene_synonym="Acry-1; Crya-1; Crya1; DAcry-1; lop18"
                     /inference="alignment:Splign:2.1.0"
     exon            383..1127
                     /gene="Cryaa"
                     /gene_synonym="Acry-1; Crya-1; Crya1; DAcry-1; lop18"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1097..1102
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Cryaa"
                     /gene_synonym="Acry-1; Crya-1; Crya1; DAcry-1; lop18"
                     /note="hexamer: AATAAA"
     polyA_site      1121
                     /gene="Cryaa"
                     /gene_synonym="Acry-1; Crya-1; Crya1; DAcry-1; lop18"
                     /note="major polyA site"
ORIGIN      
        1 ccaccgcact gcccagaggc tcctgtctga ctcactgcca gccttcggtc cctgcatcac
       61 caaatagaag atggacgtca ccattcagca tccttggttc aagcgtgccc tggggccctt
      121 ctaccccagc cgactgttcg accagttctt cggcgagggc ctttttgagt acgacctgct
      181 gcccttcctg tcttccacca tcagccccta ctaccgccag tccctcttcc gcactgtgct
      241 ggactcgggc atctctgagg tccgatctga ccgggacaag tttgtcatct tcttggacgt
      301 gaagcacttc tctcctgagg acctcaccgt gaaggtactg gaggattttg tggagattca
      361 cggcaaacac aacgagaggc aggatgacca tggctacatt tcccgtgaat ttcaccgtcg
      421 ctaccgtctg ccttccaatg tggaccagtc cgccctctcc tgctccctgt ctgcggatgg
      481 catgctgacc ttctctggcc ccaaggtcca gtccggtttg gatgctggcc acagcgagag
      541 ggccattcct gtgtcacggg aggagaaacc cagctctgca ccctcgtcct gagctggcct
      601 caccttggtt gtcccctgag gcccctggtc catccagccc agggaccaca gcaaagagtc
      661 tgccttcctg ccttcctgcc ttcctttctt tctctgtttc cttcccactt tctcagaggg
      721 ctgagggtct gagagagtgg cttagagagc ttgggctctt ggcctgagat tgccgcgggt
      781 tcagggtgac ccaggctcaa caccagctgg tcggagggaa tgatggtatt gaactcttga
      841 gattttctgt cttcctggaa agtggcatct gagcactgct ccagagggca gagtgaatgg
      901 tggctcacca actccaagag ccctgtgcta gagcccctga tggcagcctc ctcctttttg
      961 cccatactta ctgcagccac gtgtgctggg ctccgacagc cagcttctct catatcctct
     1021 tcctgtgact ttctctactc tgtagtattg ctcctgagga ccctggtcat ccatgagaat
     1081 ggagcccctg gcagacaata aagagcaggt gacagaagca atttcag
//
