LOCUS       NM_009963               4019 bp    mRNA    linear   ROD 19-AUG-2024
DEFINITION  Mus musculus cryptochrome circadian regulator 2 (Cry2), mRNA.
ACCESSION   NM_009963 XM_916214 XM_924163 XM_924166 XM_924169
VERSION     NM_009963.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4019)
  AUTHORS   Luo,S., Guo,L., Chen,N., Guo,Q., Xie,Y., Wang,Y. and Wang,E.
  TITLE     CRY2 mediates the cognitive decline induced by sleep deprivation in
            5xFAD mice
  JOURNAL   PLoS One 19 (7), e0306930 (2024)
   PUBMED   39012854
  REMARK    GeneRIF: CRY2 mediates the cognitive decline induced by sleep
            deprivation in 5xFAD mice.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4019)
  AUTHORS   Parlak,G.C., Baris,I., Gul,S. and Kavakli,I.H.
  TITLE     Functional characterization of the CRY2 circadian clock component
            variant p.Ser420Phe revealed a new degradation pathway for CRY2
  JOURNAL   J Biol Chem 299 (12), 105451 (2023)
   PUBMED   37951306
  REMARK    GeneRIF: Functional characterization of the CRY2 circadian clock
            component variant p.Ser420Phe revealed a new degradation pathway
            for CRY2.
REFERENCE   3  (bases 1 to 4019)
  AUTHORS   Lin,L., Huang,Y., Wang,J., Guo,X., Yu,F., He,D., Wu,C., Guo,L. and
            Wu,B.
  TITLE     CRY1/2 regulate rhythmic CYP2A5 in mouse liver through repression
            of E4BP4
  JOURNAL   Biochem Pharmacol 217, 115843 (2023)
   PUBMED   37797722
  REMARK    GeneRIF: CRY1/2 regulate rhythmic CYP2A5 in mouse liver through
            repression of E4BP4.
REFERENCE   4  (bases 1 to 4019)
  AUTHORS   Deng,M., Wang,Z., Luo,J., Cao,H., Li,Y., Chen,L. and Liu,G.
  TITLE     CircZNF367 promotes osteoclast differentiation and osteoporosis by
            interacting with FUS to maintain CRY2 mRNA stability
  JOURNAL   J Orthop Surg Res 18 (1), 492 (2023)
   PUBMED   37434265
  REMARK    GeneRIF: CircZNF367 promotes osteoclast differentiation and
            osteoporosis by interacting with FUS to maintain CRY2 mRNA
            stability.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 4019)
  AUTHORS   Hitrec,T., Petit,C., Cryer,E., Muir,C., Tal,N., Fustin,J.M.,
            Hughes,A.T.L. and Piggins,H.D.
  TITLE     Timed exercise stabilizes behavioral rhythms but not molecular
            programs in the brain's suprachiasmatic clock
  JOURNAL   iScience 26 (2), 106002 (2023)
   PUBMED   36866044
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 4019)
  AUTHORS   van der Horst,G.T., Muijtjens,M., Kobayashi,K., Takano,R.,
            Kanno,S., Takao,M., de Wit,J., Verkerk,A., Eker,A.P., van
            Leenen,D., Buijs,R., Bootsma,D., Hoeijmakers,J.H. and Yasui,A.
  TITLE     Mammalian Cry1 and Cry2 are essential for maintenance of circadian
            rhythms
  JOURNAL   Nature 398 (6728), 627-630 (1999)
   PUBMED   10217146
REFERENCE   7  (bases 1 to 4019)
  AUTHORS   Thresher,R.J., Vitaterna,M.H., Miyamoto,Y., Kazantsev,A., Hsu,D.S.,
            Petit,C., Selby,C.P., Dawut,L., Smithies,O., Takahashi,J.S. and
            Sancar,A.
  TITLE     Role of mouse cryptochrome blue-light photoreceptor in circadian
            photoresponses
  JOURNAL   Science 282 (5393), 1490-1494 (1998)
   PUBMED   9822380
REFERENCE   8  (bases 1 to 4019)
  AUTHORS   Kobayashi,K., Kanno,S., Smit,B., van der Horst,G.T., Takao,M. and
            Yasui,A.
  TITLE     Characterization of photolyase/blue-light receptor homologs in
            mouse and human cells
  JOURNAL   Nucleic Acids Res 26 (22), 5086-5092 (1998)
   PUBMED   9801304
REFERENCE   9  (bases 1 to 4019)
  AUTHORS   Miyamoto,Y. and Sancar,A.
  TITLE     Vitamin B2-based blue-light photoreceptors in the
            retinohypothalamic tract as the photoactive pigments for setting
            the circadian clock in mammals
  JOURNAL   Proc Natl Acad Sci U S A 95 (11), 6097-6102 (1998)
   PUBMED   9600923
REFERENCE   10 (bases 1 to 4019)
  AUTHORS   van Noort,J.M., van Sechel,A.C., Bajramovic,J.J., el Ouagmiri,M.,
            Polman,C.H., Lassmann,H. and Ravid,R.
  TITLE     The small heat-shock protein alpha B-crystallin as candidate
            autoantigen in multiple sclerosis
  JOURNAL   Nature 375 (6534), 798-801 (1995)
   PUBMED   7596414
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CJ164467.1, AK172994.1 and BC054794.1.
            
            On Jan 9, 2008 this sequence version replaced NM_009963.3.
            
            Summary: This gene encodes a flavin adenine dinucleotide-binding
            protein that is a key component of the circadian core oscillator
            complex, which regulates the circadian clock. This gene is
            upregulated by Clock/Arntl heterodimers but then represses this
            upregulation in a feedback loop using Per/Cry heterodimers to
            interact with Clock/Arntl. Polymorphisms in this gene have been
            associated with altered sleep patterns. The encoded protein is
            widely conserved across plants and animals. [provided by RefSeq,
            Feb 2014].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC054794.1, AK133781.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849380, SAMN01164131
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: inferred from homology
            RefSeq Select criteria             :: based on conservation,
                                                  expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-428               CJ164467.1         1-428
            429-3998            AK172994.1         361-3930
            3999-4019           BC054794.1         3955-3975
FEATURES             Location/Qualifiers
     source          1..4019
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2"
     gene            1..4019
                     /gene="Cry2"
                     /gene_synonym="D130054K12Rik"
                     /note="cryptochrome circadian regulator 2"
                     /db_xref="GeneID:12953"
                     /db_xref="MGI:MGI:1270859"
     exon            1..258
                     /gene="Cry2"
                     /gene_synonym="D130054K12Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             47..1825
                     /gene="Cry2"
                     /gene_synonym="D130054K12Rik"
                     /note="cryptochrome 2 (photolyase-like)"
                     /codon_start=1
                     /product="cryptochrome-2"
                     /protein_id="NP_034093.1"
                     /db_xref="CCDS:CCDS16447.1"
                     /db_xref="GeneID:12953"
                     /db_xref="MGI:MGI:1270859"
                     /translation="MAAAAVVAATVPAQSMGADGASSVHWFRKGLRLHDNPALLAAVR
                     GARCVRCVYILDPWFAASSSVGINRWRFLLQSLEDLDTSLRKLNSRLFVVRGQPADVF
                     PRLFKEWGVTRLTFEYDSEPFGKERDAAIMKMAKEAGVEVVTENSHTLYDLDRIIELN
                     GQKPPLTYKRFQALISRMELPKKPAVAVSSQQMESCRAEIQENHDDTYGVPSLEELGF
                     PTEGLGPAVWQGGETEALARLDKHLERKAWVANYERPRMNANSLLASPTGLSPYLRFG
                     CLSCRLFYYRLWDLYKKVKRNSTPPLSLFGQLLWREFFYTAATNNPRFDRMEGNPICI
                     QIPWDRNPEALAKWAEGKTGFPWIDAIMTQLRQEGWIHHLARHAVACFLTRGDLWVSW
                     ESGVRVFDELLLDADFSVNAGSWMWLSCSAFFQQFFHCYCPVGFGRRTDPSGDYIRRY
                     LPKLKGFPSRYIYEPWNAPESVQKAAKCIIGVDYPRPIVNHAETSRLNIERMKQIYQQ
                     LSRYRGLCLLASVPSCVEDLSHPVAEPGSSQAGSISNTGPRALSSGPASPKRKLEAAE
                     EPPGEELTKRARVTEMPTQEPASKDS"
     exon            259..367
                     /gene="Cry2"
                     /gene_synonym="D130054K12Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            368..510
                     /gene="Cry2"
                     /gene_synonym="D130054K12Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            511..695
                     /gene="Cry2"
                     /gene_synonym="D130054K12Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            696..784
                     /gene="Cry2"
                     /gene_synonym="D130054K12Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            785..925
                     /gene="Cry2"
                     /gene_synonym="D130054K12Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            926..1237
                     /gene="Cry2"
                     /gene_synonym="D130054K12Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1238..1389
                     /gene="Cry2"
                     /gene_synonym="D130054K12Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1390..1592
                     /gene="Cry2"
                     /gene_synonym="D130054K12Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1593..1685
                     /gene="Cry2"
                     /gene_synonym="D130054K12Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1686..1827
                     /gene="Cry2"
                     /gene_synonym="D130054K12Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1828..4002
                     /gene="Cry2"
                     /gene_synonym="D130054K12Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3974..3979
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Cry2"
                     /gene_synonym="D130054K12Rik"
                     /note="hexamer: TATAAA"
     polyA_site      4002
                     /gene="Cry2"
                     /gene_synonym="D130054K12Rik"
                     /note="major polyA site"
ORIGIN      
        1 ggggcggtcg ggcggagcgg cagaccgaga cccagtccag gcggcgatgg cggcggctgc
       61 tgtggtggca gcgacggtcc ccgcgcaatc gatgggcgcg gacggcgcgt cctcggtgca
      121 ctggttccgc aaaggactac ggctccacga caaccccgcg ctgctagctg ccgtgcgcgg
      181 ggcgcgctgt gtgcgctgcg tctacatcct cgacccgtgg ttcgcggcct cctcgtctgt
      241 gggcatcaac cgatggaggt tcctactgca atctctggaa gatctggaca caagcttaag
      301 aaagctgaat tcgcgtctgt ttgtagtccg gggacagcca gctgatgtgt tcccaaggct
      361 gttcaaggaa tggggggtga cccgtttgac ctttgaatat gactctgaac cctttgggaa
      421 agaacgggat gcagccatta tgaagatggc caaggaggct ggcgtggagg tggtgactga
      481 gaactctcac accctctatg acctagacag aatcatcgaa ctgaatgggc agaaaccacc
      541 ccttacctac aagcgctttc aggccctcat cagccgcatg gagctgccca agaagcccgc
      601 ggtggctgtg agcagccagc agatggagag ctgcagagct gagatccagg agaaccatga
      661 cgacacctat ggcgtgcctt ccctggagga gctgggattc cccacggaag gacttggccc
      721 agctgtttgg caaggaggag agacagaagc tctggcccgc ctggacaagc acttggaacg
      781 gaaggcctgg gttgccaact atgagagacc tcggatgaat gccaattcct tactggccag
      841 ccccacaggc ctcagcccct acctgcgctt tggatgcctc tcctgccgcc tcttctacta
      901 ccgcctgtgg gacttgtaca agaaggtgaa gaggaacagc acaccccccc tctccttatt
      961 tggacaactc ctgtggcgag aattcttcta cacagcggcc accaacaacc ccaggtttga
     1021 ccgaatggag gggaacccca tctgcatcca gatcccctgg gaccgcaacc ccgaagccct
     1081 ggccaagtgg gccgagggca agacaggctt cccttggatt gacgccatca tgacccaact
     1141 gaggcaggag ggctggatcc accacctggc ccggcacgct gtggcctgct tcctcacccg
     1201 cggggacctc tgggtcagct gggagagcgg ggtccgggta tttgacgagc tgctcctgga
     1261 tgccgatttc agtgtgaatg caggcagctg gatgtggctg tcctgcagtg ctttcttcca
     1321 acaattcttc cactgctact gccctgtggg cttcggccga cgtacagacc ccagtgggga
     1381 ctacatccgg cggtacctgc ccaaactgaa aggcttcccc tctcgataca tctatgagcc
     1441 ctggaatgcc cccgagtcag ttcagaaggc tgccaagtgc atcattggcg tggactaccc
     1501 acggcccatc gtcaatcatg cagagactag tcggctcaac attgaacgaa tgaagcagat
     1561 ctaccaacag ctgtcgagat accggggact ctgtctattg gcatctgtcc cttcctgtgt
     1621 ggaagacctc agtcaccctg tggcagagcc tggttcaagc caagctggga gcatcagcaa
     1681 cacaggcccc agagcactat ccagtggccc agcttccccc aaacgcaagc tggaagcagc
     1741 cgaggaacct cctggtgaag aactgaccaa gcgggctaga gtgacggaga tgcctaccca
     1801 agagccagca agcaaggact cctgagactg gagagccatc gctccgtgag caaagcccgg
     1861 gtgcctgagc taccatggcc acagagtgca atctgcagag aagccaatca ccaacagacg
     1921 gagcgagcga gtgtgtgcgt gtttgcagag gaaggtgtgg tgtgccattt gtgtgtgcat
     1981 gcatctgttt acactctcat gatcctgaat gttgcctgcg ctggaggagt ccctagatcg
     2041 tatcttctta ccagggctgc ttcttgacct ccagacacaa gactagaagc cacagtagtg
     2101 actttcagcc caaatctgtc cctgggagcc ccttccttgt cccagtgagg tggtagtacc
     2161 ctagctcaaa ttctggcctc tgcctccctt gatttttctt cctccctcct cctttcagac
     2221 aaggagctgg agggccacat tttttgactc tcatctgaag catagagttt cagaggcagc
     2281 cagagtcctg ctgacttagt tcccatgttc tgacactaga acctgagcag gctggagtac
     2341 cagtcttgta aaactccata ctactagcag gcttccctga tgtctgagac aactgactgg
     2401 tgtgctgtgt gcccatgctg cttatgtcag cctcaaacag caaatctgtc tgtgtagaac
     2461 cttctagaag ccaagtggag aatagactcc caggcttctg tcccttcccc cgcagctaag
     2521 gcccagtctc ccaccttcta gaagccgtga acaggccagg gtctgagccg gtggaagaaa
     2581 tcctcgtccc ttggctgaag gcccctggtt cctctggcca tgctcctgac acgacttgct
     2641 ccagcaagtc ctgctgctac ccagccaggc aagcgggaca gtctgctctg caggcttcca
     2701 gggccggccc tggaccaatc agcacccaca ctgcagtgtc agtccaggac tgagcttcag
     2761 gggactcaga gagactgaga gtgaagcact gcctggggca gttggtggaa ggtcagagat
     2821 cacacctggc acaccaacct cttctcagag cagcagtgga aggtttggcc tagagaagtc
     2881 aagcctcact ttccagggga gccaactctc ccgcccactg ctgggtctta tggagaaaga
     2941 agttatgaaa agaaggatct gggggcacct gagcaagtct tccttctacc ccgtggcctg
     3001 cacttgagcc acagtgtatg tgtggggaga aagagaaaga gtgtgtatgt gcgtgagaga
     3061 gtgtgtgtgt gtgtgagaga gagagagcgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt
     3121 gtgtgtgtgt gtgtgctgtt tggatttttg ttctcacatg taacattaag ctggcctctg
     3181 ggccttttcc tttctacctc ccctgtgacc tttcctagcc tcagagttgt taatgccctt
     3241 ggccctggcc tttatcatgt cagaccagaa gcctggggtc agactcgcct ctccagctgt
     3301 ctagtacatc tgacaggctt ctttttgaga tggcctcagg ttttctcagc agagagctgc
     3361 ctttagtcca actgtttgtg tccatcattc tgactagaag catcctatga ttgatcgggt
     3421 gaagaaacag ggcacctgtg atgtccatgt ttagagttct ggggtgtaac ctccctgctc
     3481 cttagcccag gccaagagga aggagccaaa ccgccttgct ggcgagaagg tagagtgggc
     3541 ctgaaaaacc agtctcagga gcaaaggcta attctgcgtc caggatttag tttgcaacat
     3601 cttcagctgt ggggacaatg gccttctccg ctttttttga tgatggttct cttcacaagg
     3661 tataagttgg caagatcatc tgtcccccca cctccttgac aagctggccc attcccaggc
     3721 cagccttcca atgcaaggag cagtctacac ccacagctga gatagagtgg cctggagcct
     3781 tggggtgact tcatgcctct ttttgcctag cctgagtggc cctgaggact gtcacagaac
     3841 agtgccccat gtcctcctcc tgggacctag catggggaag agatggttgc aggcaagaag
     3901 cactttacag agttccctat tgctgggaag gttgtgtttc tcccacagtt tgtttgtgaa
     3961 tatttacctg ttttataaat gtctgatcct gtctgagtaa aaaaaaaaaa aaaaaaaaa
//
