LOCUS       NM_001033638             887 bp    mRNA    linear   ROD 02-JUN-2024
DEFINITION  Mus musculus cone-rod homeobox, opposite strand (Crxos), transcript
            variant 1, mRNA.
ACCESSION   NM_001033638 XM_133196 XM_895520 XM_914356
VERSION     NM_001033638.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 887)
  AUTHORS   Cui,W., Dai,X., Marcho,C., Han,Z., Zhang,K., Tremblay,K.D. and
            Mager,J.
  TITLE     Towards Functional Annotation of the Preimplantation Transcriptome:
            An RNAi Screen in Mammalian Embryos
  JOURNAL   Sci Rep 6, 37396 (2016)
   PUBMED   27869233
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 887)
  AUTHORS   Sato,S., Nakazawa,M., Kihara,Y., Kubo,Y., Sato,Y., Kikuchi,T.,
            Nonaka,A., Sasaki,A., Iwashita,J., Murata,J., Hosaka,M. and
            Kobayashi,M.
  TITLE     Partial inhibition of differentiation associated with elevated
            protein levels of pluripotency factors in mouse embryonic stem
            cells expressing exogenous EGAM1N homeoprotein
  JOURNAL   J Biosci Bioeng 120 (5), 562-569 (2015)
   PUBMED   25817697
  REMARK    GeneRIF: Expression of EGAM1N could inhibit differentiation by
            elevating the protein levels of pluripotency factors in mouse ES
            cells.
REFERENCE   3  (bases 1 to 887)
  AUTHORS   Thompson,C.L., Ng,L., Menon,V., Martinez,S., Lee,C.K.,
            Glattfelder,K., Sunkin,S.M., Henry,A., Lau,C., Dang,C.,
            Garcia-Lopez,R., Martinez-Ferre,A., Pombero,A., Rubenstein,J.L.R.,
            Wakeman,W.B., Hohmann,J., Dee,N., Sodt,A.J., Young,R., Smith,K.,
            Nguyen,T.N., Kidney,J., Kuan,L., Jeromin,A., Kaykas,A., Miller,J.,
            Page,D., Orta,G., Bernard,A., Riley,Z., Smith,S., Wohnoutka,P.,
            Hawrylycz,M.J., Puelles,L. and Jones,A.R.
  TITLE     A high-resolution spatiotemporal atlas of gene expression of the
            developing mouse brain
  JOURNAL   Neuron 83 (2), 309-323 (2014)
   PUBMED   24952961
REFERENCE   4  (bases 1 to 887)
  AUTHORS   Sato,S., Morita,S., Iha,M., Mori,Y., Sugawara,S., Kasuga,K.,
            Kojima,I., Ozaki,N., Muraguchi,H., Okano,K., Iwashita,J.,
            Murata,J., Hosaka,M. and Kobayashi,M.
  TITLE     Intact structure of EGAM1 homeoproteins and basic amino acid
            residues in the common homeodomain of EGAM1 and EGAM1C contribute
            to their nuclear localization in mouse embryonic stem cells
  JOURNAL   J Biosci Bioeng 116 (2), 141-146 (2013)
   PUBMED   23510645
  REMARK    GeneRIF: Nuclear localization of exogenously expressed EGAM1
            homeoproteins (EGAM1, EGAM1N, and EGAM1C) was determined.  The
            amino acid residues crucial for the nuclear translocation of EGAM1
            and EGAM1C were identified.
REFERENCE   5  (bases 1 to 887)
  AUTHORS   Iha,M., Soma,M., Sato,S., Mori,Y., Sugawara,S., Kasuga,K.,
            Kojima,I., Yamada,S., Sakaki,S. and Kobayashi,M.
  TITLE     Severe inhibition of in vitro cardiomyogenesis in mouse embryonic
            stem cells ectopically expressing EGAM1C homeoprotein
  JOURNAL   Biosci Biotechnol Biochem 76 (7), 1410-1412 (2012)
   PUBMED   22785471
  REMARK    GeneRIF: The generation of cardiomyocytes was severely suppressed
            in Egam1c transfectants without upregulation of Nkx2-5, a crucial
            gene for cardiomyogenesis. These results indicate that EGAM1C is
            capable of affecting terminal differentiation in mouse ES cells.
REFERENCE   6  (bases 1 to 887)
  AUTHORS   Saito,K., Ogawa,A., Toyofuku,K., Hosoi,Y., Soma,M., Iha,M.,
            Kasuga,K., Kojima,I. and Kobayashi,M.
  TITLE     Relationships between homeoprotein EGAM1C and the expression of the
            placental prolactin gene family in mouse placentae and trophoblast
            stem cells
  JOURNAL   Reproduction 141 (2), 259-268 (2011)
   PUBMED   21084569
  REMARK    GeneRIF: EGAM1C may play a role in the expression of members of the
            placental PRL gene family, such as Prl3b1 and Prl5a1.
REFERENCE   7  (bases 1 to 887)
  AUTHORS   Jelcick,A.S., Yuan,Y., Leehy,B.D., Cox,L.C., Silveira,A.C., Qiu,F.,
            Schenk,S., Sachs,A.J., Morrison,M.A., Nystuen,A.M., DeAngelis,M.M.
            and Haider,N.B.
  TITLE     Genetic variations strongly influence phenotypic outcome in the
            mouse retina
  JOURNAL   PLoS One 6 (7), e21858 (2011)
   PUBMED   21779340
  REMARK    Erratum:[PLoS One. 2011;6(10).
            doi:10.1371/annotation/f375292d-9fc7-4e3c-9dc7-af0aee76fa15]
REFERENCE   8  (bases 1 to 887)
  AUTHORS   Saito,K., Abe,H., Nakazawa,M., Irokawa,E., Watanabe,M., Hosoi,Y.,
            Soma,M., Kasuga,K., Kojima,I. and Kobayashi,M.
  TITLE     Cloning of complementary DNAs encoding structurally related
            homeoproteins from preimplantation mouse embryos: their involvement
            in the differentiation of embryonic stem cells
  JOURNAL   Biol Reprod 82 (4), 687-697 (2010)
   PUBMED   20018908
  REMARK    GeneRIF: these transcripts encoding homeoproteins are capable of
            regulating the maintenance and/or differentiation of mouse ES cells
            and likely regulate that of preimplantation embryos.
REFERENCE   9  (bases 1 to 887)
  AUTHORS   Saito,R., Yamasaki,T., Nagai,Y., Wu,J., Kajiho,H., Yokoi,T.,
            Noda,E., Nishina,S., Niwa,H., Azuma,N., Katada,T. and Nishina,H.
  TITLE     CrxOS maintains the self-renewal capacity of murine embryonic stem
            cells
  JOURNAL   Biochem Biophys Res Commun 390 (4), 1129-1135 (2009)
   PUBMED   19800316
  REMARK    GeneRIF: this is the first delineation of the physiological role of
            CrxOS in ES cells.
REFERENCE   10 (bases 1 to 887)
  AUTHORS   Alfano,G., Vitiello,C., Caccioppoli,C., Caramico,T., Carola,A.,
            Szego,M.J., McInnes,R.R., Auricchio,A. and Banfi,S.
  TITLE     Natural antisense transcripts associated with genes involved in eye
            development
  JOURNAL   Hum Mol Genet 14 (7), 913-923 (2005)
   PUBMED   15703187
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC148990.6.
            
            On Oct 21, 2022 this sequence version replaced NM_001033638.2.
            
            Transcript Variant: This variant (1) encodes the longest isoform
            (a, also known as Egam1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AB472692.1, AK145701.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849376,
                                           SAMN00849379 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-87                AC148990.6         78098-78184
            88-236              AC148990.6         79331-79479
            237-408             AC148990.6         80372-80543
            409-566             AC148990.6         84777-84934
            567-887             AC148990.6         85627-85947
FEATURES             Location/Qualifiers
     source          1..887
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="7"
                     /map="7 8.6 cM"
     gene            1..887
                     /gene="Crxos"
                     /gene_synonym="Crxos1; Egam1"
                     /note="cone-rod homeobox, opposite strand"
                     /db_xref="GeneID:546024"
                     /db_xref="MGI:MGI:2451355"
     exon            1..87
                     /gene="Crxos"
                     /gene_synonym="Crxos1; Egam1"
                     /inference="alignment:Splign:2.1.0"
     CDS             63..803
                     /gene="Crxos"
                     /gene_synonym="Crxos1; Egam1"
                     /note="isoform a is encoded by transcript variant 1; Crx
                     opposite strand transcript 1; expressing gene at morula
                     stage protein 1; cone-rod homeobox, opposite strand
                     protein; homeobox protein Crxos"
                     /codon_start=1
                     /product="homeobox protein Crxos isoform a"
                     /protein_id="NP_001028810.1"
                     /db_xref="CCDS:CCDS20841.1"
                     /db_xref="GeneID:546024"
                     /db_xref="MGI:MGI:2451355"
                     /translation="MEASPRSLTSCTLGPLDQKFSWEQLSELEAYFKVEPYPDLQDRK
                     IMATRLKLKEEQVEAWFIQRSLEEEMRPPLARLQQSALDGTSSPSHKALCCRPPSWKY
                     RLIPINPPESSTSCLKDSKTVLISKTELTDEQFQKLRKHFETDRCPNEETLQAFAEEL
                     KLRKDLIRSWFITQRHRMRGYRRLFMRYYRDWKTSREYSTTRSFDRQKNSKECSQNDP
                     GLPEALEALKRLKLSSGYQSRDGMSQDF"
     exon            88..236
                     /gene="Crxos"
                     /gene_synonym="Crxos1; Egam1"
                     /inference="alignment:Splign:2.1.0"
     exon            237..408
                     /gene="Crxos"
                     /gene_synonym="Crxos1; Egam1"
                     /inference="alignment:Splign:2.1.0"
     exon            409..566
                     /gene="Crxos"
                     /gene_synonym="Crxos1; Egam1"
                     /inference="alignment:Splign:2.1.0"
     exon            567..887
                     /gene="Crxos"
                     /gene_synonym="Crxos1; Egam1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      859..864
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Crxos"
                     /gene_synonym="Crxos1; Egam1"
                     /note="hexamer: AATAAA"
     polyA_site      887
                     /gene="Crxos"
                     /gene_synonym="Crxos1; Egam1"
                     /note="major polyA site"
ORIGIN      
        1 agtttgaatc ctaaatcctt agagcatacc ttcaatcaca actgaagtga gctacaggcg
       61 aaatggaagc atctccacgt tccctgacct cctgtaccct gggtcccctt gaccagaagt
      121 tcagttggga gcagctttca gagctggaag catacttcaa ggtggaacca tacccagacc
      181 tccaggatcg aaagatcatg gccaccagac tgaaactgaa agaggaacaa gttgaggctt
      241 ggttcatcca gagatccttg gaggaggaga tgcggccccc cttggccagg ctgcagcagt
      301 ctgccctgga tggtacctct tctccttccc acaaagccct gtgctgcagg ccaccaagct
      361 ggaagtacag gctcattccc atcaatcccc ctgagtcctc gaccagctgc ttaaaggata
      421 gcaaaactgt attaatttca aagacggagt tgaccgatga gcagttccag aagctaagaa
      481 agcacttcga gacagaccgt tgccctaatg aggaaactct gcaggccttc gctgaggaac
      541 tcaaactacg gaaagacctc atcagatcct ggttcatcac tcagcgtcac agaatgaggg
      601 ggtacagacg gttatttatg cgctactaca gggattggaa aacgtcccgt gagtactcta
      661 caacaagaag tttcgacagg cagaagaact ccaaggagtg ctctcagaat gaccctggac
      721 tccccgaagc attggaagcc ttgaagaggc tcaagctctc ttctgggtac caaagcagag
      781 atggcatgtc acaagatttc tgacttttca acttcttggt gggggcaggc gtctgtctat
      841 gggagaaggt acattaataa taaagcgtgc attcatagag tcgaaaa
//
