LOCUS       NM_023733               2803 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus carnitine O-octanoyltransferase (Crot), transcript
            variant 1, mRNA.
ACCESSION   NM_023733
VERSION     NM_023733.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2803)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2803)
  AUTHORS   Lasheras-Otero,I., Feliu,I., Maillo,A., Moreno,H.,
            Redondo-Munoz,M., Aldaz,P., Bocanegra,A., Olias-Arjona,A.,
            Lecanda,F., Fernandez-Irigoyen,J., Santamaria,E., Larrayoz,I.M.,
            Gomez-Cabrero,D., Wellbrock,C., Vicent,S. and Arozarena,I.
  TITLE     The Regulators of Peroxisomal Acyl-Carnitine Shuttle CROT and CRAT
            Promote Metastasis in Melanoma
  JOURNAL   J Invest Dermatol 143 (2), 305-316 (2023)
   PUBMED   36058299
  REMARK    GeneRIF: The Regulators of Peroxisomal Acyl-Carnitine Shuttle CROT
            and CRAT Promote Metastasis in Melanoma.
REFERENCE   3  (bases 1 to 2803)
  AUTHORS   Okui,T., Iwashita,M., Rogers,M.A., Halu,A., Atkins,S.K.,
            Kuraoka,S., Abdelhamid,I., Higashi,H., Ramsaroop,A., Aikawa,M.,
            Singh,S.A. and Aikawa,E.
  TITLE     CROT (Carnitine O-Octanoyltransferase) Is a Novel Contributing
            Factor in Vascular Calcification via Promoting Fatty Acid
            Metabolism and Mitochondrial Dysfunction
  JOURNAL   Arterioscler Thromb Vasc Biol 41 (2), 755-768 (2021)
   PUBMED   33356393
  REMARK    GeneRIF: CROT (Carnitine O-Octanoyltransferase) Is a Novel
            Contributing Factor in Vascular Calcification via Promoting Fatty
            Acid Metabolism and Mitochondrial Dysfunction.
REFERENCE   4  (bases 1 to 2803)
  AUTHORS   Fan,Z., Zhao,M., Joshi,P.D., Li,P., Zhang,Y., Guo,W., Xu,Y.,
            Wang,H., Zhao,Z. and Yan,J.
  TITLE     A class of circadian long non-coding RNAs mark enhancers modulating
            long-range circadian gene regulation
  JOURNAL   Nucleic Acids Res 45 (10), 5720-5738 (2017)
   PUBMED   28335007
REFERENCE   5  (bases 1 to 2803)
  AUTHORS   Labialle,S., Marty,V., Bortolin-Cavaille,M.L., Hoareau-Osman,M.,
            Pradere,J.P., Valet,P., Martin,P.G. and Cavaille,J.
  TITLE     The miR-379/miR-410 cluster at the imprinted Dlk1-Dio3 domain
            controls neonatal metabolic adaptation
  JOURNAL   EMBO J 33 (19), 2216-2230 (2014)
   PUBMED   25124681
REFERENCE   6  (bases 1 to 2803)
  AUTHORS   Gineste,C., De Winter,J.M., Kohl,C., Witt,C.C., Giannesini,B.,
            Brohm,K., Le Fur,Y., Gretz,N., Vilmen,C., Pecchi,E., Jubeau,M.,
            Cozzone,P.J., Stienen,G.J., Granzier,H., Labeit,S.,
            Ottenheijm,C.A., Bendahan,D. and Gondin,J.
  TITLE     In vivo and in vitro investigations of heterozygous nebulin
            knock-out mice disclose a mild skeletal muscle phenotype
  JOURNAL   Neuromuscul Disord 23 (4), 357-369 (2013)
   PUBMED   23375831
REFERENCE   7  (bases 1 to 2803)
  AUTHORS   Kim,S.C., Sprung,R., Chen,Y., Xu,Y., Ball,H., Pei,J., Cheng,T.,
            Kho,Y., Xiao,H., Xiao,L., Grishin,N.V., White,M., Yang,X.J. and
            Zhao,Y.
  TITLE     Substrate and functional diversity of lysine acetylation revealed
            by a proteomics survey
  JOURNAL   Mol Cell 23 (4), 607-618 (2006)
   PUBMED   16916647
REFERENCE   8  (bases 1 to 2803)
  AUTHORS   Jogl,G., Hsiao,Y.S. and Tong,L.
  TITLE     Crystal structure of mouse carnitine octanoyltransferase and
            molecular determinants of substrate selectivity
  JOURNAL   J Biol Chem 280 (1), 738-744 (2005)
   PUBMED   15492013
  REMARK    GeneRIF: the crystal structures of carnitine octanoyltransferase
            alone and in complex with the substrate octanoylcarnitine
REFERENCE   9  (bases 1 to 2803)
  AUTHORS   Caudevilla,C., Codony,C., Serra,D., Plasencia,G., Roman,R.,
            Graessmann,A., Asins,G., Bach-Elias,M. and Hegardt,F.G.
  TITLE     Localization of an exonic splicing enhancer responsible for
            mammalian natural trans-splicing
  JOURNAL   Nucleic Acids Res 29 (14), 3108-3115 (2001)
   PUBMED   11452036
REFERENCE   10 (bases 1 to 2803)
  AUTHORS   Farrell,S.O., Fiol,C.J., Reddy,J.K. and Bieber,L.L.
  TITLE     Properties of purified carnitine acyltransferases of mouse liver
            peroxisomes
  JOURNAL   J Biol Chem 259 (21), 13089-13095 (1984)
   PUBMED   6436243
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK004567.2.
            
            On Apr 6, 2007 this sequence version replaced NM_023733.2.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK004567.2, BC012308.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849386 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2803              AK004567.2         2-2804
FEATURES             Location/Qualifiers
     source          1..2803
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="5"
                     /map="5 3.5 cM"
     gene            1..2803
                     /gene="Crot"
                     /gene_synonym="1200003H03Rik"
                     /note="carnitine O-octanoyltransferase"
                     /db_xref="GeneID:74114"
                     /db_xref="MGI:MGI:1921364"
     exon            1..43
                     /gene="Crot"
                     /gene_synonym="1200003H03Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            44..139
                     /gene="Crot"
                     /gene_synonym="1200003H03Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            140..275
                     /gene="Crot"
                     /gene_synonym="1200003H03Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             161..1999
                     /gene="Crot"
                     /gene_synonym="1200003H03Rik"
                     /EC_number="2.3.1.137"
                     /note="isoform a is encoded by transcript variant 1;
                     peroxisomal carnitine O-octanoyltransferase; COT"
                     /codon_start=1
                     /product="peroxisomal carnitine O-octanoyltransferase
                     isoform a"
                     /protein_id="NP_076222.1"
                     /db_xref="CCDS:CCDS19087.1"
                     /db_xref="GeneID:74114"
                     /db_xref="MGI:MGI:1921364"
                     /translation="MENQLTKSVEERTFQYQDSLPSLPVPALEESLKKYLESVKPFAN
                     EDEYKKTEEIVQKFQEGAGKRLHQKLLERARGKRNWLEEWWLNVAYLDVRIPSQLNVN
                     FVGPCPHFEHYWPAREGTQLERGSMMLWHNLNYWQLLRREKLPVHKSGNTPLDMNQFR
                     MLFSTCKVPGITRDSIMNYFKTESEGHCPTHIAVLCRGRAFVFDVLHEGCLITPPELL
                     RQLTYIHKKCSNEPVGPSIAALTSEERTRWAKAREYLISLDPENLTLLEKIQTSLFVY
                     SIEDSSPHATPEEYSQVFEMLLGGDPSVRWGDKSYNLISFANGIFGCCCDHAPYDAMV
                     MVNIAHYVDERVLETEGRWKGSEKVRDIPLPEELVFTVDEKILNDVSQAKAQHLKAAS
                     DLQIAASTFTSFGKKLTKEEALHPDTFIQLALQLAYYRLHGRPGCCYETAMTRYFYHG
                     RTETVRSCTVEAVRWCQSMQDPSASLLERQQKMLEAFAKHNKMMKDCSHGKGFDRHLL
                     GLLLIAKEEGLPVPELFEDPLFSRSGGGGNFVLSTSLVGYLRVQGVVVPMVHNGYGFF
                     YHIRDDRFVVACSSWRSCPETDAEKLVQMIFHAFHDMIQLMNTAHL"
     exon            276..400
                     /gene="Crot"
                     /gene_synonym="1200003H03Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            401..582
                     /gene="Crot"
                     /gene_synonym="1200003H03Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            583..707
                     /gene="Crot"
                     /gene_synonym="1200003H03Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            708..816
                     /gene="Crot"
                     /gene_synonym="1200003H03Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            817..910
                     /gene="Crot"
                     /gene_synonym="1200003H03Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            911..1036
                     /gene="Crot"
                     /gene_synonym="1200003H03Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1037..1138
                     /gene="Crot"
                     /gene_synonym="1200003H03Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1139..1222
                     /gene="Crot"
                     /gene_synonym="1200003H03Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1223..1330
                     /gene="Crot"
                     /gene_synonym="1200003H03Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1331..1461
                     /gene="Crot"
                     /gene_synonym="1200003H03Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1462..1585
                     /gene="Crot"
                     /gene_synonym="1200003H03Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1586..1664
                     /gene="Crot"
                     /gene_synonym="1200003H03Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1665..1758
                     /gene="Crot"
                     /gene_synonym="1200003H03Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1759..1878
                     /gene="Crot"
                     /gene_synonym="1200003H03Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1879..2803
                     /gene="Crot"
                     /gene_synonym="1200003H03Rik"
                     /inference="alignment:Splign:2.1.0"
     polyA_site      2803
                     /gene="Crot"
                     /gene_synonym="1200003H03Rik"
                     /note="putative"
ORIGIN      
        1 agagcctgca ggccgacgca gagggacaag aggggcgcgg gagtcaccag gcttggccag
       61 ttcgggttcc ctgtggtttc ctttccgaat tgttcaagca ggctcctgga ggtcctgagc
      121 acttcttcgt ggtttactgt tctcttactg tgactttatc atggaaaatc aattgactaa
      181 atcagttgaa gaacggacat ttcagtacca ggactctctt ccgtcattgc ctgttcctgc
      241 acttgaagaa tcattaaaaa aataccttga atcagtgaaa ccattcgcaa atgaagacga
      301 gtataagaaa actgaagaaa tagttcaaaa gtttcaagaa ggagctggca aaagattgca
      361 tcagaagtta cttgaaaggg ctagaggaaa acgaaactgg ctggaagaat ggtggctcaa
      421 tgttgcctac ctggacgtac gtattccatc acaactgaat gtgaactttg tgggtccgtg
      481 tcctcatttt gagcactact ggcctgcaag ggaaggcact cagttagaaa gaggaagcat
      541 gatgctgtgg cacaacttga actactggca gctgctaaga agagaaaaat tgcctgtaca
      601 taaatctgga aatactcctc tagacatgaa ccaattccga atgttgtttt ctacctgcaa
      661 ggttccagga attactagag attcgattat gaattatttt aagactgaga gtgaagggca
      721 ttgtccaacc cacattgccg tgctgtgtcg aggcagagcg tttgtctttg atgtactaca
      781 tgagggatgt ttgatcaccc caccagaact tctcagacaa ttgacatata tccacaagaa
      841 gtgctcgaac gaacctgttg gacccagtat agcggcatta actagcgagg agcgaactcg
      901 gtgggcaaag gcaagagaat atctgattag tcttgatcca gagaacttga ctttattaga
      961 aaaaattcaa accagtttat ttgtgtattc catagaagat agcagtccac atgcaacccc
     1021 agaagaatat tctcaggtct ttgaaatgct tcttggtgga gatccatcag tacgctgggg
     1081 tgacaagtcc tataatttga tttcctttgc taatggaata tttggctgtt gctgtgatca
     1141 tgctccttac gatgcaatgg tcatggtgaa cattgcacat tatgttgatg agagagtcct
     1201 agagacagaa ggaagatgga agggttcaga aaaagtccgg gacataccat tgccagagga
     1261 gctggtcttc actgtggatg agaaaatact gaatgatgta tcccaagcca aagcccaaca
     1321 tctcaaagct gcgtcagatc tgcagatagc agcatctacc ttcacatctt ttggcaaaaa
     1381 gctcaccaag gaggaagccc ttcaccctga tacctttatt cagctcgctc ttcagcttgc
     1441 ctactacaga cttcatggac gccctggttg ctgctatgag acagctatga caagatactt
     1501 ttaccacggc cgaacagaga ctgtgcgatc ttgtacagtg gaggccgtca ggtggtgcca
     1561 gtccatgcag gatccctctg ctagtctcct tgaacgtcag caaaagatgc tagaagcttt
     1621 tgccaagcat aataagatga tgaaagattg ttcacatgga aaaggatttg accgtcacct
     1681 tttaggtctt ttgctcatag caaaagagga aggtctccct gttccagaac tatttgagga
     1741 cccacttttc tccagaagtg gaggaggtgg aaattttgtg ctctcaacaa gtttggttgg
     1801 ttacttacga gttcagggtg ttgtggttcc catggtacac aatggatatg gatttttcta
     1861 ccacatcaga gatgacaggt ttgtggtggc atgttcatcc tggaggtcat gtccggagac
     1921 tgatgctgaa aagcttgttc agatgatttt tcatgctttc cacgatatga tacagctgat
     1981 gaacaccgct catctttaga gactcagaca aatacagatc atagaaactt ggtacgaaga
     2041 gtaggatggt gatgtgacat ggaaggaatg ttgacttaaa ggaagcctgt taatgtaggg
     2101 ttaggagagt gatgctctct agagttattc taccttaaag tctcctgtgt tgcaaccaca
     2161 atgcaaagtc agatatagtg aatagaacta tgcaatgttt aagcctcaac aaagcaaatc
     2221 tatatatctt aacaatccta cttaacaagc ctcaatcaaa gcaaatccta ctctgatgtt
     2281 aaaatagttt tgttggcaca tgtgtaggct gcaagtcctc tgtgaacatg attatagagt
     2341 ctatttctca agcactttaa tactttctaa ttgccagagg gataaagcac acggttagaa
     2401 gctaatttct ctgacatcag tgccttccac acccagcaca ggagtacagc ctgtgagagt
     2461 tcatgggaaa accactattg tacaatagtg atctaaaata gctcctttga gcaggcaaaa
     2521 ggatcaagtt gtattagaaa tttttacact accacagaaa agaatattag caaaactcat
     2581 tatgatatgt tgcaattcat tttgtgaata taaaattaaa gcatttccat ttgaacctaa
     2641 ttgttagagc tagcagcttt taaagggtta aatgttgagt atgattctca gaactttaca
     2701 attatttccc agtgttattc ataatggtgg cttgaagacg ttcgcttcaa gggtgctatt
     2761 caaagtggtt tatattctca aagaataaag catccagaat ctt
//
