LOCUS       NM_007764               5050 bp    mRNA    linear   ROD 18-JUN-2024
DEFINITION  Mus musculus v-crk avian sarcoma virus CT10 oncogene homolog-like
            (Crkl), transcript variant 1, mRNA.
ACCESSION   NM_007764 XM_358774
VERSION     NM_007764.5
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 5050)
  AUTHORS   Vong,K.I., Lee,S., Au,K.S., Crowley,T.B., Capra,V., Martino,J.,
            Haller,M., Araujo,C., Machado,H.R., George,R., Gerding,B.,
            James,K.N., Stanley,V., Jiang,N., Alu,K., Meave,N., Nidhiry,A.S.,
            Jiwani,F., Tang,I., Nisal,A., Jhamb,I., Patel,A., Patel,A.,
            McEvoy-Venneri,J., Barrows,C., Shen,C., Ha,Y.J., Howarth,R.,
            Strain,M., Ashley-Koch,A.E., Azam,M., Mumtaz,S., Bot,G.M.,
            Finnell,R.H., Kibar,Z., Marwan,A.I., Melikishvili,G., Meltzer,H.S.,
            Mutchinick,O.M., Stevenson,D.A., Mroczkowski,H.J., Ostrander,B.,
            Schindewolf,E., Moldenhauer,J., Zackai,E.H., Emanuel,B.S.,
            Garcia-Minaur,S., Nowakowska,B.A., Stevenson,R.E., Zaki,M.S.,
            Northrup,H., McNamara,H.K., Aldinger,K.A., Phelps,I.G., Deng,M.,
            Glass,I.A., Morrow,B., McDonald-McGinn,D.M., Sanna-Cherchi,S.,
            Lamb,D.J. and Gleeson,J.G.
  CONSRTM   Spina Bifida Sequencing Consortiumdouble dagger
  TITLE     Risk of meningomyelocele mediated by the common 22q11.2 deletion
  JOURNAL   Science 384 (6695), 584-590 (2024)
   PUBMED   38696583
REFERENCE   2  (bases 1 to 5050)
  AUTHORS   Wu,B., Wu,B., Benkaci,S., Shi,L., Lu,P., Park,T., Morrow,B.E.,
            Wang,Y. and Zhou,B.
  TITLE     Crk and Crkl Are Required in the Endocardial Lineage for Heart
            Valve Development
  JOURNAL   J Am Heart Assoc 12 (18), e029683 (2023)
   PUBMED   37702066
  REMARK    GeneRIF: Crk and Crkl Are Required in the Endocardial Lineage for
            Heart Valve Development.
REFERENCE   3  (bases 1 to 5050)
  AUTHORS   Handel,A.E., Cheuk,S., Dhalla,F., Maio,S., Hubscher,T., Rota,I.,
            Deadman,M.E., Ekwall,O., Lutolf,M., Weinberg,K. and Hollander,G.
  TITLE     Developmental dynamics of the neural crest-mesenchymal axis in
            creating the thymic microenvironment
  JOURNAL   Sci Adv 8 (19), eabm9844 (2022)
   PUBMED   35559672
REFERENCE   4  (bases 1 to 5050)
  AUTHORS   Shi,L., Racedo,S.E., Diacou,A., Park,T., Zhou,B. and Morrow,B.E.
  TITLE     Crk and Crkl have shared functions in neural crest cells for
            cardiac outflow tract septation and vascular smooth muscle
            differentiation
  JOURNAL   Hum Mol Genet 31 (8), 1197-1215 (2022)
   PUBMED   34686881
REFERENCE   5  (bases 1 to 5050)
  AUTHORS   Hemmeryckx,B., Reichert,A., Watanabe,M., Kaartinen,V., de Jong,R.,
            Pattengale,P.K., Groffen,J. and Heisterkamp,N.
  TITLE     BCR/ABL P190 transgenic mice develop leukemia in the absence of
            Crkl
  JOURNAL   Oncogene 21 (20), 3225-3231 (2002)
   PUBMED   12082638
REFERENCE   6  (bases 1 to 5050)
  AUTHORS   Guris,D.L., Fantes,J., Tara,D., Druker,B.J. and Imamoto,A.
  TITLE     Mice lacking the homologue of the human 22q11.2 gene CRKL phenocopy
            neurocristopathies of DiGeorge syndrome
  JOURNAL   Nat Genet 27 (3), 293-298 (2001)
   PUBMED   11242111
  REMARK    GeneRIF: Crkol is essential for cranial and cardiac neural crest
            development. The chromosomal location of the human CRKL gene
            (22q11.21) and the phenotype of Crkol mutant mice suggest that
            defects in CRKL-mediated pathways underlie DiGeorge syndrome.
REFERENCE   7  (bases 1 to 5050)
  AUTHORS   Sattler,M., Verma,S., Pride,Y.B., Salgia,R., Rohrschneider,L.R. and
            Griffin,J.D.
  TITLE     SHIP1, an SH2 domain containing polyinositol-5-phosphatase,
            regulates migration through two critical tyrosine residues and
            forms a novel signaling complex with DOK1 and CRKL
  JOURNAL   J Biol Chem 276 (4), 2451-2458 (2001)
   PUBMED   11031258
REFERENCE   8  (bases 1 to 5050)
  AUTHORS   Oda,T., Kujovich,J., Reis,M., Newman,B. and Druker,B.J.
  TITLE     Identification and characterization of two novel SH2
            domain-containing proteins from a yeast two hybrid screen with the
            ABL tyrosine kinase
  JOURNAL   Oncogene 15 (11), 1255-1262 (1997)
   PUBMED   9315092
REFERENCE   9  (bases 1 to 5050)
  AUTHORS   Glass,D.J., Bowen,D.C., Stitt,T.N., Radziejewski,C., Bruno,J.,
            Ryan,T.E., Gies,D.R., Shah,S., Mattsson,K., Burden,S.J.,
            DiStefano,P.S., Valenzuela,D.M., DeChiara,T.M. and Yancopoulos,G.D.
  TITLE     Agrin acts via a MuSK receptor complex
  JOURNAL   Cell 85 (4), 513-523 (1996)
   PUBMED   8653787
REFERENCE   10 (bases 1 to 5050)
  AUTHORS   de Jong,R., Haataja,L., Voncken,J.W., Heisterkamp,N. and Groffen,J.
  TITLE     Tyrosine phosphorylation of murine Crkl
  JOURNAL   Oncogene 11 (8), 1469-1474 (1995)
   PUBMED   7478571
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AK052315.1, AK048641.1,
            AW541802.2, BF453354.1, BF464821.1 and BY471344.1.
            
            On Mar 23, 2013 this sequence version replaced NM_007764.4.
            
            Summary: This gene is part of a family of adapter proteins that
            mediate formation of signal transduction complexes in response to
            extracellular stimuli, such as growth and differentiation factors.
            Protein-protein interactions occur through the SH2 domain, which
            binds phosphorylated tyrosine residues, and the SH3 domain, which
            binds proline-rich peptide motifs. These interactions promote
            recruitment and activation of effector proteins to regulate cell
            migration, adhesion, and proliferation. In certain mouse genetic
            backgrounds this protein is essential for embryonic development. It
            is important for neural crest cell differentiation and survival and
            is proposed to play an important role in transducing the oncogenic
            signal of Bcr/Abl. Deletion of this gene in mouse mimics the
            phenotype of DiGeorge/velocardiofacial syndrome in human.
            Alternative splicing results in multiple transcript variants that
            encode different protein isoforms. [provided by RefSeq, Mar 2013].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK052315.1, SRR1660815.35856.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-356               AK052315.1         1-356
            357-3865            AK048641.1         355-3863
            3866-4347           AW541802.2         104-585             c
            4348-4739           BF453354.1         102-493
            4740-5043           BF464821.1         18-321              c
            5044-5050           BY471344.1         483-489
FEATURES             Location/Qualifiers
     source          1..5050
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="16"
                     /map="16 10.82 cM"
     gene            1..5050
                     /gene="Crkl"
                     /gene_synonym="1110025F07Rik; Crkol; snoop"
                     /note="v-crk avian sarcoma virus CT10 oncogene
                     homolog-like"
                     /db_xref="GeneID:12929"
                     /db_xref="MGI:MGI:104686"
     exon            1..804
                     /gene="Crkl"
                     /gene_synonym="1110025F07Rik; Crkol; snoop"
                     /inference="alignment:Splign:2.1.0"
     CDS             494..1405
                     /gene="Crkl"
                     /gene_synonym="1110025F07Rik; Crkol; snoop"
                     /note="isoform 1 is encoded by transcript variant 1;
                     crk-like protein; v-crk sarcoma virus CT10 oncogene
                     homolog-like"
                     /codon_start=1
                     /product="crk-like protein isoform 1"
                     /protein_id="NP_031790.2"
                     /db_xref="CCDS:CCDS28002.1"
                     /db_xref="GeneID:12929"
                     /db_xref="MGI:MGI:104686"
                     /translation="MSSARFDSSDRSAWYMGPVTRQEAQTRLQGQRHGMFLVRDSSTC
                     PGDYVLSVSENSRVSHYIINSLPNRRFKIGDQEFDHLPALLEFYKIHYLDTTTLIEPA
                     PRYPSPPVGSVSAPNLPTAEENLEYVRTLYDFPGNDAEDLPFKKGELLVIIEKPEEQW
                     WSARNKDGRVGMIPVPYVEKLVRSSPHGKHGNRNSNSYGIPEPAHAYAQPQTTTPLPT
                     VASTPGAAINPLPSTQNGPVFAKAIQKRVPCAYDKTALALEVGDIVKVTRMNINGQWE
                     GEVNGRKGLFPFTHVKIFDPQNPDDNE"
     exon            805..1270
                     /gene="Crkl"
                     /gene_synonym="1110025F07Rik; Crkol; snoop"
                     /inference="alignment:Splign:2.1.0"
     exon            1271..5050
                     /gene="Crkl"
                     /gene_synonym="1110025F07Rik; Crkol; snoop"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5013..5018
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Crkl"
                     /gene_synonym="1110025F07Rik; Crkol; snoop"
                     /note="hexamer: AATAAA"
     polyA_site      5043
                     /gene="Crkl"
                     /gene_synonym="1110025F07Rik; Crkol; snoop"
                     /note="major polyA site"
ORIGIN      
        1 gggaggtgcc cggctgcttc tcccgcgtcc gccattttgt tgctgtggct attgggagcg
       61 ggctgggtaa gactgctcgg gaggcgcgac tcttgttgga gtgcggtgct tcgtgtgacc
      121 gcggggatcg gtgcaggctc ttgaggctgt ggagccggcg gcgggttcca ggacagaagt
      181 cactggagcc tcccctgtac tccggccagc tgagaagccc gaggcggcct ccgctactgc
      241 tcagcttcgc ccacctcgac cccggggctt tgcccagtgt tcccggaccg gctctccgtg
      301 aggcggcccg gtgcaggagg gcggtggcgg aggatgctgc ggggcccgga gcctagagga
      361 acgtcctgac caagccctct gattgttcct cgaagtgttc tgagaggccc ttcctcgacc
      421 ccaaagccga cagcgggtaa aggcttccca agcaagagag ccgcagacgc cgcccatccc
      481 cgagcccaac accatgtcct ccgccaggtt tgattcttca gaccgctctg cctggtacat
      541 ggggccagtg actcgccagg aggcgcagac tcgtctccaa ggccagcgcc atggcatgtt
      601 cctagtccgg gactcatcta cctgccccgg ggactatgta ctgtccgtgt ccgagaactc
      661 gcgtgtctcg cactacatca tcaactccct gcccaaccgc cgctttaaga tcggggacca
      721 ggagtttgac catttgccgg ccttgttaga gttctacaag atccactacc tggacactac
      781 caccttaatc gaaccggcgc ccaggtaccc aagcccacca gtgggttctg tctcagcacc
      841 caacttacct acagcagaag aaaatctgga atatgtacgg accctttatg attttcctgg
      901 gaatgatgct gaagacctac cctttaaaaa gggcgagctt ctagtgataa tagaaaagcc
      961 tgaagagcag tggtggagtg cccgcaacaa ggacggccgg gttgggatga ttcctgtccc
     1021 ttacgttgaa aagcttgtga ggtcttcgcc acatggaaag catggaaata ggaattctaa
     1081 cagttatggc atcccagaac ctgctcatgc gtatgctcaa cctcagacca caactcctct
     1141 acctacagtt gccagtactc ctggggcagc gatcaaccct ttgccatcca cacagaatgg
     1201 acctgtcttt gcaaaagcaa tccagaagag agtaccttgt gcttatgaca agactgcctt
     1261 ggcattggag gttggtgaca ttgtgaaagt cacaaggatg aatatcaatg gccaatggga
     1321 aggcgaggtg aatgggcgca aggggctttt ccccttcaca catgttaaaa tctttgaccc
     1381 tcagaacccc gatgacaacg agtgattgct gtgctgtccc tgctgctgcc tccttctgcc
     1441 tgtcagtctc ctttgaagtg ggaagcactc tgtcacatgc aagttacacc gagctgctgg
     1501 cggtcagctt tccatacatt ggcagtccct gcacatacgg aatgcctcag cagctgcctt
     1561 gctcgctggc tcggcatttg tatcatagtc atgctgtcaa agagtagctg atttagagtt
     1621 cttgtggatt gtgaactgga aacactggtg gaagcacaca gtagagactg gaacccagag
     1681 cagctcttcc ttcccatcca tcactgcctt gtttgtactg gaggctgaca gagagagcaa
     1741 gcaagcctgc tgatggtctg catggagacg gtgaggcttg gcaaggtctc gctttgctgg
     1801 tccacagaac agtggttttg ccaccaactc agttttcttt ggacgacaga ggaagtaaaa
     1861 ctccagacaa ttctgtcccg aactcctcaa gtctggctgg ttttcatttt cttttgttta
     1921 gttttgtttt ttggaagact aattaattat agttgtgtgt tctgaacaga tttttaaata
     1981 gtaggttggt ctatgatggt ctaaggaaca atccatgtct ctgagcttcc aaactgaagc
     2041 cactgtacgt aactaaaata ggaaaagacc ccctccaggg gcctttatct tttggttgcc
     2101 acagtacctt gtttaccatg tggcaaacag tgcacagcag cagtcatggt gtaccttgct
     2161 gtgaagcacc aaggtggggg tgggcctgct gtcatcgctg cctctgacag gagcagacag
     2221 ggtgggcctc ttcagtgaaa acgtttgaag tctgtgggct tactagatcc gtgagttaaa
     2281 ttgcattgct gtgttccacc acagaggcca cccagccctt cctgtactga cctagcttgt
     2341 ccaggcagct tgagagtggt gtctgttctg gttccacagc ctgagtagaa ccagaataca
     2401 gtgcttctga gcaaaagaag acgcccctcg atcatgagcc atttttcctt gcatgtcagc
     2461 tactacagat atgtgtctga tccctcatcc acacatggca aaccagagct ctagaacctt
     2521 tctagagtgc actcttttca gttggaccct gatgaggata cacctccaca cagaacgaac
     2581 gtagcagacc tagagatgga gtagtggcta gtgagcagct ctagcattta cagaatatct
     2641 tctgtaacca tggccctggt tctgcaggcc tgaggctttg agaactaatg gaacatattc
     2701 aggtgacaaa accaacaaaa ccctttgtcc actatattta tttctcttaa agactttaaa
     2761 tttttgttta tgttgggcat ggtggacaca cctttaatcc cagtactcag aaaggcaaag
     2821 gcaagtggat ctctgagagt tcaaggctat cctgatctat gtagtgtgtc acaggacagg
     2881 caggcctaca tacatacctt gtatcagaag atggcccaat ccccccctca gacctccccc
     2941 caaaaaaaac tgtatatggg tgtttccact acatgtatgt atgtgtatca catgggtagt
     3001 ttgttaggag gccagaaaag ggtgttggtt ggatcctctg gaactgtgga gttactctgt
     3061 gggttctagg aattgaacct gggtcctctg caagaagacc ttaaccactg agccatttct
     3121 tgagccccat tagtttctct ttaaatcagt agtccatcag gctaagttct atagtaaaga
     3181 atgttcatgt gacaaagtaa tggtcatttg gggtatgggg agtgtcacgt ctgtagaggt
     3241 tacttctttt tgtctctgaa agctgccatt tctagccaga cctattgagt ggacttaagt
     3301 gttcaggccc cagactctgc ctaaatcaag atttaagact tgccttctgg ccagttacac
     3361 agtttagccc caggcctttg atgtctccac tggaatacac accttgttca ctgagggcac
     3421 aacccccaag ggatacccca tcactggggc ctgaacctca tgccttacac gtgctagata
     3481 accggctgcc agtaagcttt tgaaatccga gatgggatct ctataaattg cccatgctgg
     3541 cttcaagatt tccatcccag cctcagctga aattataaag gtttgagtca ccacacccag
     3601 ctcttaaggt cttttaaaac tacatttaac tgaagggctt ggtgcctttt gattatagca
     3661 ccttgcttag cataaacagc catttttgag aacagggttt cccttttccc aagactgttg
     3721 accagcaaga ctctaaggaa tacttcatga tcagaatgtc ccataaccct ggagtttgct
     3781 ttatgtaatc agttaatttg gcacatgtaa atgtctatgc agcttcctca gagatccaga
     3841 tctcaaaata aagacttaac tttctgaact ctcaggatac gtgcgtgcgt gccaacaata
     3901 gtaattctct gacttagaag ctcatcgatt tttaatcatg gaaatctctc tcactgatag
     3961 aaagcagcat cttccccaag tcccactctt ctgggaagac ccttggtttc aggggagtac
     4021 gatggaggct tccttttctc atcatcagtg ctgtgtggtc tgctctgcca tgttgtagaa
     4081 agtactggga tgataaaccc acttgctatg gctgttgtat ctgagaagga aacaattagg
     4141 tagttgaggg ctttaagggc tagttagtcc tgggtgacag ccccccacct ccacctccaa
     4201 ccccatacag aaggcatgct tgtcactctg cactgctctg atgccacctg tttcgaggtt
     4261 gacagggtgt cctggctttt gtcatccaga cgtaaccctc tgtgctcact ggaagagact
     4321 ctgccctctt ttcaagttgc acgatcctgg cactgtgcca gccttcctct acagctgttt
     4381 acagacaaga tgggccaaga ctgatggcca ctgctcttgt aacctttact caaaagaata
     4441 atgagcattt aaaaaaaaaa aaagggatga tgtgtttttg ttccaggtgt tcataattta
     4501 atcttttaat attatgttca ttaacctctt aaaatgagtg aattcttgat tgttttaaca
     4561 cagtacctaa gactaatgct ttctgtggac tcctcactct ggaaccagtg aacccctccc
     4621 tggttaactg ctgtgagcgt ggacgctgag ctgcttgtat attctgaagg agcaggagga
     4681 cagttctgag acaggaggac agtggccttg cttctagacg gtcttcactg tgtgttttaa
     4741 aatgacataa aactcttttg acgtgtataa cttaaagaac ataagcatca ggcaataata
     4801 ttttctgtgt aagcttttaa aattattttt ggggatcata gcttgtttta ttttgtgcta
     4861 taaaattaac agtattaaat gacttatatt cttagaatac attgagtgtc ttttcttaac
     4921 agattagtgc ctttttattt ttgtattcca ttttgcgtta ctggtcccag catcaaaacc
     4981 cttgtttcca tggcctgttt gtacattgtc tcaataaaac ttgcaccagc cggcgtggca
     5041 gcagcctcgg
//
