LOCUS       NM_133656               6005 bp    mRNA    linear   ROD 05-JUN-2024
DEFINITION  Mus musculus v-crk avian sarcoma virus CT10 oncogene homolog (Crk),
            transcript variant 2, mRNA.
ACCESSION   NM_133656
VERSION     NM_133656.5
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 6005)
  AUTHORS   Wu,B., Wu,B., Benkaci,S., Shi,L., Lu,P., Park,T., Morrow,B.E.,
            Wang,Y. and Zhou,B.
  TITLE     Crk and Crkl Are Required in the Endocardial Lineage for Heart
            Valve Development
  JOURNAL   J Am Heart Assoc 12 (18), e029683 (2023)
   PUBMED   37702066
  REMARK    GeneRIF: Crk and Crkl Are Required in the Endocardial Lineage for
            Heart Valve Development.
REFERENCE   2  (bases 1 to 6005)
  AUTHORS   Shi,L., Racedo,S.E., Diacou,A., Park,T., Zhou,B. and Morrow,B.E.
  TITLE     Crk and Crkl have shared functions in neural crest cells for
            cardiac outflow tract septation and vascular smooth muscle
            differentiation
  JOURNAL   Hum Mol Genet 31 (8), 1197-1215 (2022)
   PUBMED   34686881
REFERENCE   3  (bases 1 to 6005)
  AUTHORS   Kanazawa,T., Michida,H., Uchino,Y., Ishihara,A., Zhang,S.,
            Tabata,S., Suzuki,Y., Imamoto,A. and Okada,M.
  TITLE     Cell shape-based chemical screening reveals an epigenetic network
            mediated by focal adhesions
  JOURNAL   FEBS J 288 (19), 5613-5628 (2021)
   PUBMED   33768715
  REMARK    GeneRIF: Cell shape-based chemical screening reveals an epigenetic
            network mediated by focal adhesions.
REFERENCE   4  (bases 1 to 6005)
  AUTHORS   Antoku,S. and Mayer,B.J.
  TITLE     Distinct roles for Crk adaptor isoforms in actin reorganization
            induced by extracellular signals
  JOURNAL   J Cell Sci 122 (Pt 22), 4228-4238 (2009)
   PUBMED   19861495
REFERENCE   5  (bases 1 to 6005)
  AUTHORS   Park,T.J., Boyd,K. and Curran,T.
  TITLE     Cardiovascular and craniofacial defects in Crk-null mice
  JOURNAL   Mol Cell Biol 26 (16), 6272-6282 (2006)
   PUBMED   16880535
  REMARK    GeneRIF: These data indicate that Crk is involved in cardiac and
            craniofacial development and that it plays an essential role in
            maintaining vascular integrity during embryonic development.
REFERENCE   6  (bases 1 to 6005)
  AUTHORS   de Jong,R., Haataja,L., Voncken,J.W., Heisterkamp,N. and Groffen,J.
  TITLE     Tyrosine phosphorylation of murine Crkl
  JOURNAL   Oncogene 11 (8), 1469-1474 (1995)
   PUBMED   7478571
REFERENCE   7  (bases 1 to 6005)
  AUTHORS   Wu,X., Knudsen,B., Feller,S.M., Zheng,J., Sali,A., Cowburn,D.,
            Hanafusa,H. and Kuriyan,J.
  TITLE     Structural basis for the specific interaction of lysine-containing
            proline-rich peptides with the N-terminal SH3 domain of c-Crk
  JOURNAL   Structure 3 (2), 215-226 (1995)
   PUBMED   7735837
REFERENCE   8  (bases 1 to 6005)
  AUTHORS   Ogawa,S., Toyoshima,H., Kozutsumi,H., Hagiwara,K., Sakai,R.,
            Tanaka,T., Hirano,N., Mano,H., Yazaki,Y. and Hirai,H.
  TITLE     The C-terminal SH3 domain of the mouse c-Crk protein negatively
            regulates tyrosine-phosphorylation of Crk associated p130 in rat
            3Y1 cells
  JOURNAL   Oncogene 9 (6), 1669-1678 (1994)
   PUBMED   8183562
REFERENCE   9  (bases 1 to 6005)
  AUTHORS   Margolis,B., Silvennoinen,O., Comoglio,F., Roonprapunt,C.,
            Skolnik,E., Ullrich,A. and Schlessinger,J.
  TITLE     High-efficiency expression/cloning of epidermal growth
            factor-receptor-binding proteins with Src homology 2 domains
  JOURNAL   Proc Natl Acad Sci U S A 89 (19), 8894-8898 (1992)
   PUBMED   1409582
  REMARK    GeneRIF: The authors cloned SH2 domain-containing proteins they
            named GRBs (for growth factor receptor-bound). They identified
            GRB-3 as having 77% identity at the protein level and 80%
            similarity at the DNA level to v-crk.
REFERENCE   10 (bases 1 to 6005)
  AUTHORS   Mayer,B.J., Hamaguchi,M. and Hanafusa,H.
  TITLE     A novel viral oncogene with structural similarity to phospholipase
            C
  JOURNAL   Nature 332 (6161), 272-275 (1988)
   PUBMED   2450282
  REMARK    GeneRIF: The authors cloned and sequenced the avian sarcoma virus
            CT10 from infected QT6 quail cells then demonstrated that the
            protein p47 phosphorylates tyrosine residues and can modulate
            activity of tyrosine kinases. They name the oncogene crk for CT10
            regulator of kinase.
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL591440.7 and AK028488.1.
            
            On Mar 23, 2013 this sequence version replaced NM_133656.4.
            
            Summary: This gene is part of a family of adapter proteins that
            mediate formation of signal transduction complexes in response to
            extracellular stimuli, such as growth and differentiation factors.
            Protein-protein interactions occur through the SH2 domain, which
            binds phosphorylated tyrosine residues, and the SH3 domain, which
            binds proline-rich peptide motifs. These interactions promote
            recruitment and activation of effector proteins to regulate cell
            migration, adhesion, and proliferation. In mouse this protein is
            essential for embryonic development. Alternatively spliced
            transcripts encoding different isoforms with distinct biological
            activity have been described. [provided by RefSeq, Mar 2013].
            
            Transcript Variant: This variant (2) includes an alternate segment,
            compared to variant 1, resulting in a longer protein (isoform 2,
            also known as CrkII) that has a distinct C-terminus and an
            additional SH3 domain, compared to isoform 1 (CrkI). Isoform 2
            (CrkII) is proposed to have a negative regulatory role.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK028488.1, AK154987.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-51                AL591440.7         36789-36839         c
            52-2177             AK028488.1         2-2127
            2178-6005           AL591440.7         7670-11497          c
FEATURES             Location/Qualifiers
     source          1..6005
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="11"
                     /map="11 45.92 cM"
     gene            1..6005
                     /gene="Crk"
                     /gene_synonym="c-Crk; Crk-I; Crk-II; Crk-III; Crk3;
                     CrkIII; Crko; p38"
                     /note="v-crk avian sarcoma virus CT10 oncogene homolog"
                     /db_xref="GeneID:12928"
                     /db_xref="MGI:MGI:88508"
     exon            1..406
                     /gene="Crk"
                     /gene_synonym="c-Crk; Crk-I; Crk-II; Crk-III; Crk3;
                     CrkIII; Crko; p38"
                     /inference="alignment:Splign:2.1.0"
     CDS             166..1080
                     /gene="Crk"
                     /gene_synonym="c-Crk; Crk-I; Crk-II; Crk-III; Crk3;
                     CrkIII; Crko; p38"
                     /note="isoform 2 is encoded by transcript variant 2;
                     adapter molecule crk; CT-10 related kinase 3; v-crk
                     sarcoma virus CT10 oncogene-like protein; avian sarcoma
                     virus CT10 (v-crk) oncogene homolog; proto-oncogene c-crk"
                     /codon_start=1
                     /product="adapter molecule crk isoform 2"
                     /protein_id="NP_598417.2"
                     /db_xref="CCDS:CCDS25055.1"
                     /db_xref="GeneID:12928"
                     /db_xref="MGI:MGI:88508"
                     /translation="MAGNFDSEERSSWYWGRLSRQEAVALLQGQRHGVFLVRDSSTSP
                     GDYVLSVSENSRVSHYIINSSGPRPPVPPSPAQPPPGVSPSRLRIGDQEFDSLPALLE
                     FYKIHYLDTTTLIEPVARSRQGSGVILRQEEAEYVRALFDFNGNDEEDLPFKKGDILR
                     IRDKPEEQWWNAEDSEGKRGMIPVPYVEKYRPASASVSALIGGNQEGSHPQPLGGPEP
                     GPYAQPSVNTPLPNLQNGPIYARVIQKRVPNAYDKTALALEVGELVKVTKINVSGQWE
                     GECNGKRGHFPFTHVRLLDQQNPDEDFS"
     exon            407..942
                     /gene="Crk"
                     /gene_synonym="c-Crk; Crk-I; Crk-II; Crk-III; Crk3;
                     CrkIII; Crko; p38"
                     /inference="alignment:Splign:2.1.0"
     exon            943..6005
                     /gene="Crk"
                     /gene_synonym="c-Crk; Crk-I; Crk-II; Crk-III; Crk3;
                     CrkIII; Crko; p38"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3630..3635
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Crk"
                     /gene_synonym="c-Crk; Crk-I; Crk-II; Crk-III; Crk3;
                     CrkIII; Crko; p38"
                     /note="hexamer: AATAAA"
     polyA_site      3647
                     /gene="Crk"
                     /gene_synonym="c-Crk; Crk-I; Crk-II; Crk-III; Crk3;
                     CrkIII; Crko; p38"
                     /note="major polyA site"
     polyA_site      3678
                     /gene="Crk"
                     /gene_synonym="c-Crk; Crk-I; Crk-II; Crk-III; Crk3;
                     CrkIII; Crko; p38"
     regulatory      4469..4474
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Crk"
                     /gene_synonym="c-Crk; Crk-I; Crk-II; Crk-III; Crk3;
                     CrkIII; Crko; p38"
                     /note="hexamer: AATAAA"
     polyA_site      4492
                     /gene="Crk"
                     /gene_synonym="c-Crk; Crk-I; Crk-II; Crk-III; Crk3;
                     CrkIII; Crko; p38"
     regulatory      5982..5987
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Crk"
                     /gene_synonym="c-Crk; Crk-I; Crk-II; Crk-III; Crk3;
                     CrkIII; Crko; p38"
                     /note="hexamer: AATAAA"
     polyA_site      6005
                     /gene="Crk"
                     /gene_synonym="c-Crk; Crk-I; Crk-II; Crk-III; Crk3;
                     CrkIII; Crko; p38"
ORIGIN      
        1 gggtagaggg cgcgtcattt ccggtgggga aggcccgcgg ccgccgccgc catttcgggc
       61 gctgctggga ggctgacacc ggagccggtc cgctgggcgg cgggcgccag ggctggaggg
      121 gcgcgcgtgc cggcggcggc ccagcgtgaa agcgcggagg cggccatggc gggcaacttc
      181 gactcggagg agcggagtag ctggtactgg ggccgcctga gccggcagga ggcggtggcg
      241 ctattgcagg gccagcggca cggggtgttc ctggtgcggg actcgagcac cagccccggg
      301 gactatgtgc ttagcgtctc cgaaaactcg cgcgtctccc actacatcat caacagcagc
      361 ggcccgcgcc ctccagtgcc tccgtcgccc gctcagcctc cgccgggagt gagtccctcc
      421 aggctccgaa taggagatca agaatttgat tcattgcctg ctttactgga attctacaaa
      481 atacactatt tggacactac aacattgata gaaccagtgg ccagatcaag gcagggtagt
      541 ggagtgattc tcaggcagga ggaggcagag tatgtgcggg ccctctttga ctttaatggg
      601 aatgatgaag aagatcttcc ctttaagaaa ggagacatcc tgagaatccg ggataagcct
      661 gaagagcagt ggtggaatgc agaggacagc gaaggaaaga gggggatgat tcctgtccct
      721 tacgtggaga agtatagacc tgcctccgcc tcagtatcgg ctctgattgg aggtaaccag
      781 gagggttccc acccacagcc actgggtggg ccggagcctg ggccctatgc ccaacccagc
      841 gtcaacactc cgctccctaa cctccagaat gggcccattt atgccagggt tatccagaag
      901 cgagtcccta atgcctacga caagacagcc ttggctttgg aggtcggtga gctggtaaag
      961 gttacgaaga ttaatgtgag tggtcagtgg gaaggggagt gtaatggcaa acgaggtcac
     1021 ttcccattca cacatgtccg tctgctggat caacagaatc ccgatgagga cttcagctga
     1081 gtatagctcg acagtttgct gacagatgga acaatctgtt tccccccaat tgccatctat
     1141 acaattttct tacaggtgtc aaagcagtct agtttatata agcattctgt tacctgggat
     1201 cttttttaag actgaactac tccattctca cttgtattta ccatattcag ggtacgaaac
     1261 cggagggctt atgtggttaa cttctgagtt ggcagtttta ggtggtagtt gccgtgcctg
     1321 tatgagaaga cgtaaataca ttgcctcctt tcttttgggc aaaacagatc aacctgtgga
     1381 aagctgatgg gcaggaaaag aatgttactc actgctgacc ctgatttctt cagaggttct
     1441 gttttcatcc tgaatccata ctgtcaagtg gcagcagact gttccccact cccatgacgt
     1501 aatcatgcac tgtgtgaata ttacccagtg gggttgcatt tgcatttatg gaccatgacc
     1561 attgtatgac tcattctgac atttcaaatc ctgaggattc tgagaacact actagaggca
     1621 tttgtcttcc ttggattttt cttggattct ttccttgtta ctctcctccc ctgtacctat
     1681 aagtggcttg attcttagga taatgtatat cttcattcca gatgagaagc aggatgtaca
     1741 gagcacttta ttcagtgcat agctttaagc cagtattgga ttcactcagt ggataaactc
     1801 ccagctctct gccttccccc aagggggcat cgtaggttca cactgctacc acagctcaag
     1861 actcacctta ctgatgggac agatctggca gggccatttt tcctgattgc tagtatccta
     1921 atgggatgct caaagttctc agattctact tggagtggaa ggaccaatca cttactgtat
     1981 cctatagaag gtatcaaggg ccaaactttg ccctctgcat catgtgcaag ttgtataaaa
     2041 ggcaggttag aattctatgc atttgggtag gattgatact ttgaaaacta ttccagtcgt
     2101 aaattactta tgtatcattt tacatggttt aatagttggt gaagtatatt acactataaa
     2161 ttgccttcat ttgggttctc ctttgtccag atttagttta caacccttta aagtagagaa
     2221 tgatttatac gaggtcagat gctccagaga ctaggcctct gaggccacaa attatagggt
     2281 ttctttagac tgtaacatga gactttcagg tgataaaggc acttgctggt ctctggtgta
     2341 ggtgaccaat agaaacacct tatactttgc tttgaacctc attttagaaa aataatttac
     2401 cttcttagtt gtcttgtgta gattaatatg atgaatttgc attcattgaa catataccaa
     2461 atcatgtgac tgaagtgact agcacattgc tgagcacgtg tgtagagatt ttgtacgaaa
     2521 gaatgagcag agtgcagaag cgtgttgtat ggcccaggga cgccagttgg catacattac
     2581 cacaggcaga tttaatgagt tgatgctaat ttaatacatt ttttacctca ctgtagcttg
     2641 ataaaaacag taatactcaa ctttagagat gttggcccag ttctgagctc ataataggtg
     2701 gtcagtgata ctgaatggac aggtttgtag cacgtgactt actgcacctg actgcttgtt
     2761 tcacttttca gttagcatag attgtgtaaa tccaaatgca cagtcaatcc tgtttcccta
     2821 agccttgtgt gatggtgaat gtctttagtc ctagcccttg ggagacaaag gcaagtggat
     2881 ctctgagttc aagaccaacc tggtctacag agctacttcc agaacagtca ggagttagaa
     2941 agtgagacct tgtttcaaaa acaaaaacaa aaacaaaaaa gaatctgttc ctgaaattat
     3001 ggcatcgaaa acacttctta gaaaatcatc gtcacaactg atttgaatgc tggtattttc
     3061 ttttgccttt gatgctggct tgtattgtct cttcactata tgtggtggga atgggatctg
     3121 cagctctccg tgaagcctac gaactcctca gtgattagtt attcagagcc attacgtgaa
     3181 ctgtttggtt ggatttggct gggtgtagca gttattggac ctcaagagat caaaggactt
     3241 cttacaaata tcttccaaaa atagcaagtg ctataatcct agtcagatgc gtatgtacat
     3301 tgtcacagag caaagtaaac tgaaattggc tgctatgttt tatataatca cttctgcaag
     3361 agcccatttt tggatactaa tatttgacat ggttaattct tattaatttg ttgaaaatgt
     3421 ttattaataa tgcaaataga taattttaat tttccacaag taacatttca ctattaatgg
     3481 tttgaaatgg gtgataagca aaccaatttg aaataaaata tgaacatgtg ccattgtatt
     3541 ataacactat tcactttctt gacagttaaa ttttaaaaat gttttttttt ttgtagcatg
     3601 tattgtatat gtttatagta tatgtagtaa ataaaagtat ggccaaatgt ttggcttggt
     3661 gatcttttga gaaagtaatc ttgaaatatt tttcacaaaa gtgagttttt gtgtttttaa
     3721 ggaatagaag gtatgtgtct cagctgttca ttagtagtta cgtaaactgt tacatggtgg
     3781 gtatgctata agatctctag aaagtatgac actactgtta ggaaacatga aaatcatata
     3841 tatggaatat aaaggaagca cattgtgaac agagaacacc agacttgcct ggaaatgggt
     3901 ctctgtgagt gtaagatggc agtgtgatag ggaactctgc aaacttgaga ctgcattctc
     3961 tgttgtgaga atgctgctgt ggatacgcca ttcatttctc ctttttaatg taagtagcaa
     4021 ttgctcaggg aaagtcctag gggaggaaca gcagcagaga tgatgcgttt ctgtgtgtcc
     4081 ataccacccg tccccagagt gcagtgcact ccacattgac actactcact ccagtattgg
     4141 agctgtggct caagtttcaa ggtatggtgg cctgtgctga taattccagg acttctgaag
     4201 ccatgggatt ccttgaaccc aggagttcaa ggtcagctta ggcaacttag caagactcct
     4261 gtatactaaa tacctaacag acaaatttta cctattattg cctatatgat actctgcctt
     4321 agactcagcc tcagcctcct ggaggctgcc acagatgtag gcctgtttag tgcagagcta
     4381 agggaaaatg ccgcctccag gggatcccgc tttagcatat gcaaaattaa cagcaaggtg
     4441 gaaagtgttt tccagcttct gacaataaaa taaactgtcc accgctttac tatgttgaat
     4501 gtcgggttcc tttacaaagt ctcagtgttc atacagccca tgtctcgctc agggaacttc
     4561 atctggaggg aggggtgcct gcataggagg ggtgggaaga ggcccagagc ggatggtggg
     4621 agctgcagga gctaccacgc atgctataat gaaagtgagc tagggtcaat cgctcatccc
     4681 gccctggaca gtctccttca gtcttcctca tggtccagct ccctgtccac acattcacat
     4741 acccttagag atgttctcaa acccatttac cttcatggtg ctcctttcaa atagtactcc
     4801 cccagcccca caagtgtagc tcagattgtc ctggaacttg ctctgtagac caggttggcc
     4861 tcgaactcac agatatccac ccgcctcttc ctcctgagca ctgggataaa aggtgtgcgc
     4921 caccagcatc ctatgctgct tacataccat ttttgtgatg gtttagctca actaacaaca
     4981 tgagtaattt ttcagttttt acattttcat agcctaccag agtgcttggc acccaggagt
     5041 cagctgttgc tgaggactag caatgggcct caaaggaaaa gtagataccc aacactaaca
     5101 atcatggctt tggcttcagt ccccagcaac acaaaaccgt agaagtgttg aatgttttcc
     5161 tcatatgaag ttgtcttagg gtttctattg ctgtaaagag acaccaggac cacagcaact
     5221 cttggaaaat atttcattga ggctgtctta cagtttaaga ggcttagtct gttactggcc
     5281 tgattcatgc agttagacat ggtgctggag aagcagctga gaggtctaca tcttgatctg
     5341 caggctgcaa ggaagcagac tactccactt cagatacctt gagaatctga gaccctaaag
     5401 gcaacctcca cagtggcaca cttcatccag caaggccata ccttctaata gtgtcacgct
     5461 ctatgggcca agcttctaaa cacaagagtc tatggggcca ttcctattca agccaccaca
     5521 ggtgtgctgg agatacaaga gcacctcagc tgcttctcta gtgtccttgg tcctctggaa
     5581 gttcagacta ggttcagcca catttactta catggtcctt catagtcaca caaaatgctc
     5641 ttcatagttt tcgagcgggt gagtgcttcc cagccctttg tgtgtttttc tctcacagag
     5701 gggagggtaa tccagtcact ccatatcgta ttatacatat agggagtctt tacttgcttg
     5761 tctcattttt ttagttttta gctttagcca cattcaaaaa ggctgagtcc cattcctcaa
     5821 agcatcaggg gtatgtccag catactttgt tctaaaacac tatatggaaa attatatcat
     5881 gactctcaga cgtgccagct cttggatatt aacacggaga cctgttactg atagtaaaag
     5941 tgtggcttta gctggcttgt tccctatagc ttgtataacc taataaaccc gttgatacta
     6001 accta
//
