LOCUS       NM_001347048            1252 bp    mRNA    linear   ROD 05-AUG-2023
DEFINITION  Mus musculus cysteine-rich secretory protein 4 (Crisp4), transcript
            variant 3, mRNA.
ACCESSION   NM_001347048 XM_006495590
VERSION     NM_001347048.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1252)
  AUTHORS   Gaikwad AS, Nandagiri A, Potter DL, Nosrati R, O'Connor AE, Jadhav
            S, Soria J, Prabhakar R and O'Bryan MK.
  TITLE     CRISPs Function to Boost Sperm Power Output and Motility
  JOURNAL   Front Cell Dev Biol 9, 693258 (2021)
   PUBMED   34422816
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1252)
  AUTHORS   Curci L, Brukman NG, Weigel Munoz M, Rojo D, Carvajal G, Sulzyk V,
            Gonzalez SN, Rubinstein M, Da Ros VG and Cuasnicu PS.
  TITLE     Functional redundancy and compensation: Deletion of multiple murine
            Crisp genes reveals their essential role for male fertility
  JOURNAL   FASEB J 34 (12), 15718-15733 (2020)
   PUBMED   33037689
REFERENCE   3  (bases 1 to 1252)
  AUTHORS   Weigel Munoz M, Carvajal G, Curci L, Gonzalez SN and Cuasnicu PS.
  TITLE     Relevance of CRISP proteins for epididymal physiology,
            fertilization, and fertility
  JOURNAL   Andrology 7 (5), 610-617 (2019)
   PUBMED   31218833
  REMARK    Review article
REFERENCE   4  (bases 1 to 1252)
  AUTHORS   Carvajal G, Brukman NG, Weigel Munoz M, Battistone MA, Guazzone VA,
            Ikawa M, Haruhiko M, Lustig L, Breton S and Cuasnicu PS.
  TITLE     Impaired male fertility and abnormal epididymal epithelium
            differentiation in mice lacking CRISP1 and CRISP4
  JOURNAL   Sci Rep 8 (1), 17531 (2018)
   PUBMED   30510210
  REMARK    GeneRIF: These observations reveal that CRISP proteins are relevant
            for epididymal epithelium differentiation and male fertility,
            contributing to a better understanding of the fine-tuning
            mechanisms underlying sperm maturation and immunotolerance in the
            epididymis with clear implications for human epididymal physiology
            and pathology.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 1252)
  AUTHORS   Hu J, Merriner DJ, O'Connor AE, Houston BJ, Furic L, Hedger MP and
            O'Bryan MK.
  TITLE     Epididymal cysteine-rich secretory proteins are required for
            epididymal sperm maturation and optimal sperm function
  JOURNAL   Mol Hum Reprod 24 (3), 111-122 (2018)
   PUBMED   29361143
REFERENCE   6  (bases 1 to 1252)
  AUTHORS   Borges BC, Garcia-Galiano D, da Silveira Cruz-Machado S, Han X,
            Gavrilina GB, Saunders TL, Auchus RJ, Hammoud SS, Smith GD and
            Elias CF.
  TITLE     Obesity-Induced Infertility in Male Mice Is Associated With
            Disruption of Crisp4 Expression and Sperm Fertilization Capacity
  JOURNAL   Endocrinology 158 (9), 2930-2943 (2017)
   PUBMED   28911169
  REMARK    GeneRIF: The obesity-induced decline in sperm motility and
            fertilization capacity results in part from the disruption of
            epididymal CRISP4 expression and secretion.
REFERENCE   7  (bases 1 to 1252)
  AUTHORS   Turunen HT, Sipila P, Krutskikh A, Toivanen J, Mankonen H,
            Hamalainen V, Bjorkgren I, Huhtaniemi I and Poutanen M.
  TITLE     Loss of cysteine-rich secretory protein 4 (Crisp4) leads to
            deficiency in sperm-zona pellucida interaction in mice
  JOURNAL   Biol Reprod 86 (1), 1-8 (2012)
   PUBMED   21865554
  REMARK    GeneRIF: This study showed that the loss of CRISP4 leads to a
            deficiency of the spermatozoa to undergo progesterone-induced
            acrosome reaction and to a decreased fertilizing ability of the
            sperm in the in vitro fertilization conditions.
            Publication Status: Online-Only
REFERENCE   8  (bases 1 to 1252)
  AUTHORS   Gibbs GM, Orta G, Reddy T, Koppers AJ, Martinez-Lopez P, de la
            Vega-Beltran JL, Lo JC, Veldhuis N, Jamsai D, McIntyre P, Darszon A
            and O'Bryan MK.
  TITLE     Cysteine-rich secretory protein 4 is an inhibitor of transient
            receptor potential M8 with a role in establishing sperm function
  JOURNAL   Proc Natl Acad Sci U S A 108 (17), 7034-7039 (2011)
   PUBMED   21482758
  REMARK    GeneRIF: these data identify CRISP4 as an endogenous regulator of
            TRPM8 with a role in normal sperm function.
REFERENCE   9  (bases 1 to 1252)
  AUTHORS   Nolan MA, Wu L, Bang HJ, Jelinsky SA, Roberts KP, Turner TT, Kopf
            GS and Johnston DS.
  TITLE     Identification of rat cysteine-rich secretory protein 4 (Crisp4) as
            the ortholog to human CRISP1 and mouse Crisp4
  JOURNAL   Biol Reprod 74 (5), 984-991 (2006)
   PUBMED   16467491
REFERENCE   10 (bases 1 to 1252)
  AUTHORS   Jalkanen J, Huhtaniemi I and Poutanen M.
  TITLE     Mouse cysteine-rich secretory protein 4 (CRISP4): a member of the
            Crisp family exclusively expressed in the epididymis in an
            androgen-dependent manner
  JOURNAL   Biol Reprod 72 (5), 1268-1274 (2005)
   PUBMED   15673606
  REMARK    GeneRIF: Crisp4 is expressed exclusively in epithelial cells of the
            epididymis in an androgen-dependent manner, and the expression of
            the gene starts at puberty along with the onset of sperm
            maturation. (CRISP4)
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK020340.1 and AC161367.4.
            
            On Nov 15, 2016 this sequence version replaced XM_006495590.3.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK020340.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849381 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-841               AK020340.1         2-842
            842-1252            AC161367.4         46564-46974         c
FEATURES             Location/Qualifiers
     source          1..1252
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="1"
                     /map="1 5.65 cM"
     gene            1..1252
                     /gene="Crisp4"
                     /gene_synonym="9230112K08Rik"
                     /note="cysteine-rich secretory protein 4"
                     /db_xref="GeneID:78081"
                     /db_xref="MGI:MGI:1925331"
     exon            1..59
                     /gene="Crisp4"
                     /gene_synonym="9230112K08Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             50..814
                     /gene="Crisp4"
                     /gene_synonym="9230112K08Rik"
                     /note="isoform 3 is encoded by transcript variant 3"
                     /codon_start=1
                     /product="cysteine-rich secretory protein 4 isoform 3"
                     /protein_id="NP_001333977.1"
                     /db_xref="CCDS:CCDS83530.1"
                     /db_xref="GeneID:78081"
                     /db_xref="MGI:MGI:1925331"
                     /translation="MDVGMAVKFILLLFVAAFVPVVTIRPLKLDRALYNKLITESQTE
                     PQEEIVNTHNAFRRKVSPPARNMLKVSWSSAAAENARILARYCDKSDSDSLERRLPNT
                     FCGENMLMEHYPSSWSKVIEIWFNESKYFKYGEWPSTDDDIETDHYTQMVWASTYLVG
                     CDVAACRRQKAATYLYVCHYCHEGNHQDTLNMPYKEGSPCDDCPNNCEDGLCTNPCIY
                     YDEYNNCDTQVKLYGCSHPAVQPFCKASCLCTTEIK"
     exon            60..124
                     /gene="Crisp4"
                     /gene_synonym="9230112K08Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            125..259
                     /gene="Crisp4"
                     /gene_synonym="9230112K08Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            260..350
                     /gene="Crisp4"
                     /gene_synonym="9230112K08Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            351..499
                     /gene="Crisp4"
                     /gene_synonym="9230112K08Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            500..597
                     /gene="Crisp4"
                     /gene_synonym="9230112K08Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            598..686
                     /gene="Crisp4"
                     /gene_synonym="9230112K08Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            687..1252
                     /gene="Crisp4"
                     /gene_synonym="9230112K08Rik"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 agtacagaca aagaccagat ttgtatcagc acacaatttt aagagataca tggatgtggg
       61 tatggcagtg aaatttatct tgttattgtt tgttgctgcc tttgttcctg ttgtgactat
      121 cagacctctc aaactggata gagctctcta taataaactg atcacggaat cacaaactga
      181 accacaagag gaaattgtca atacccataa cgccttcagg agaaaagtgt ccccgccagc
      241 caggaacatg ctgaaagtga gttggagttc agctgctgca gagaatgcta gaatcttggc
      301 gaggtactgt gacaagtcag acagtgactc acttgagaga cgactaccaa atacattttg
      361 tggagaaaat atgcttatgg aacattatcc ctcttcctgg tcaaaagtta ttgaaatctg
      421 gttcaatgag tccaaatact tcaaatatgg agaatggcca tccacagatg atgacatcga
      481 aactgatcac tacactcaga tggtctgggc ctctacctac ctcgttggct gtgatgttgc
      541 agcttgtcgc agacaaaagg cagctacata tctttatgtg tgtcactatt gtcatgaggg
      601 aaatcatcaa gatacactaa atatgcctta taaggagggc tcaccatgtg acgattgtcc
      661 aaataactgc gaagatggac tgtgcactaa cccctgcatt tattatgatg aatacaacaa
      721 ctgtgatacg caggtaaaac tttatgggtg ctcacatcca gctgtccagc cattctgcaa
      781 ggcttcttgt ctgtgtacaa ctgaaataaa ataacctaaa gtttgctact atgctctgaa
      841 gataaagtgt gttttcaggc tcaattattt caccttaatg taatgtactg taagattttg
      901 tactgaattt cattttttta aattatgctc aagaacttaa tttttaacca tcatttatag
      961 acatcaatat attttcatca attctgtgcc cagtgcatgt aataacaaag atttaaattt
     1021 cattgtttaa tgaaagggca agtcaaatgc ttgccccaaa tttaatacat gtgatttgtg
     1081 atatgtagct aaacttaatc cagctaatga atattcttca tctgtaactt gtagtaaaat
     1141 agcttttacc taaccctttt cccaatatct ccacttccca acaaccctaa ttttttttgt
     1201 tttaatgttt aattttcatt ctattttaaa tcagacatta aaaatattca ac
//
