LOCUS       NM_001204071            1451 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus cysteine-rich secretory protein 2 (Crisp2), transcript
            variant 2, mRNA.
ACCESSION   NM_001204071
VERSION     NM_001204071.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1451)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 1451)
  AUTHORS   Gaikwad,A.S., Nandagiri,A., Potter,D.L., Nosrati,R., O'Connor,A.E.,
            Jadhav,S., Soria,J., Prabhakar,R. and O'Bryan,M.K.
  TITLE     CRISPs Function to Boost Sperm Power Output and Motility
  JOURNAL   Front Cell Dev Biol 9, 693258 (2021)
   PUBMED   34422816
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 1451)
  AUTHORS   Curci,L., Brukman,N.G., Weigel Munoz,M., Rojo,D., Carvajal,G.,
            Sulzyk,V., Gonzalez,S.N., Rubinstein,M., Da Ros,V.G. and
            Cuasnicu,P.S.
  TITLE     Functional redundancy and compensation: Deletion of multiple murine
            Crisp genes reveals their essential role for male fertility
  JOURNAL   FASEB J 34 (12), 15718-15733 (2020)
   PUBMED   33037689
REFERENCE   4  (bases 1 to 1451)
  AUTHORS   Lim,S., Kierzek,M., O'Connor,A.E., Brenker,C., Merriner,D.J.,
            Okuda,H., Volpert,M., Gaikwad,A., Bianco,D., Potter,D.,
            Prabhakar,R., Strunker,T. and O'Bryan,M.K.
  TITLE     CRISP2 Is a Regulator of Multiple Aspects of Sperm Function and
            Male Fertility
  JOURNAL   Endocrinology 160 (4), 915-924 (2019)
   PUBMED   30759213
  REMARK    GeneRIF: we revealed a role for CRISP2 in establishing the ability
            of sperm to undergo the acrosome reaction and in establishing a
            normal flagellum waveform. Crisp2-deficient sperm possess a stiff
            midpiece and are thus unable to manifest the rapid form of
            progressive motility seen in wild type sperm. As a consequence,
            Crisp2-deficient males are subfertile.
REFERENCE   5  (bases 1 to 1451)
  AUTHORS   Brukman,N.G., Miyata,H., Torres,P., Lombardo,D., Caramelo,J.J.,
            Ikawa,M., Da Ros,V.G. and Cuasnicu,P.S.
  TITLE     Fertilization defects in sperm from Cysteine-rich secretory protein
            2 (Crisp2) knockout mice: implications for fertility disorders
  JOURNAL   Mol Hum Reprod 22 (4), 240-251 (2016)
   PUBMED   26786179
  REMARK    GeneRIF: Crisp2 genetic defects in sperm from humans and mice
            result in male infertility.
REFERENCE   6  (bases 1 to 1451)
  AUTHORS   Mizuki,N., Sarapata,D.E., Garcia-Sanz,J.A. and Kasahara,M.
  TITLE     The mouse male germ cell-specific gene Tpx-1: molecular structure,
            mode of expression in spermatogenesis, and sequence similarity to
            two non-mammalian genes
  JOURNAL   Mamm Genome 3 (5), 274-280 (1992)
   PUBMED   1638086
REFERENCE   7  (bases 1 to 1451)
  AUTHORS   Nadeau,J.H., Herrmann,B., Bucan,M., Burkart,D., Crosby,J.L.,
            Erhart,M.A., Kosowsky,M., Kraus,J.P., Michiels,F., Schnattinger,A.
            et al.
  TITLE     Genetic maps of mouse chromosome 17 including 12 new anonymous DNA
            loci and 25 anchor loci
  JOURNAL   Genomics 9 (1), 78-89 (1991)
   PUBMED   1672293
REFERENCE   8  (bases 1 to 1451)
  AUTHORS   Vincek,V., Figueroa,F., Gill,T.J. 3rd, Cortese Hassett,A.L. and
            Klein,J.
  TITLE     Mapping in the mouse of the region homologous to the rat growth and
            reproduction complex (grc)
  JOURNAL   Immunogenetics 32 (4), 293-295 (1990)
   PUBMED   1978716
REFERENCE   9  (bases 1 to 1451)
  AUTHORS   Richards,S., Bucan,M., Brorson,K., Kiefer,M.C., Hunt,S.W. 3rd,
            Lehrach,H. and Lindahl,K.F.
  TITLE     Genetic and molecular mapping of the Hmt region of mouse
  JOURNAL   EMBO J 8 (12), 3749-3757 (1989)
   PUBMED   2573520
REFERENCE   10 (bases 1 to 1451)
  AUTHORS   Kasahara,M., Passmore,H.C. and Klein,J.
  TITLE     A testis-specific gene Tpx-1 maps between Pgk-2 and Mep-1 on mouse
            chromosome 17
  JOURNAL   Immunogenetics 29 (1), 61-63 (1989)
   PUBMED   2562800
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC135084.4, CJ046590.1 and BC049615.1.
            
            Transcript Variant: This variant (2) differs in the 5' UTR,
            compared to variant 1. Variants 1 and 2 encode the same protein.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR5189685.80015.1,
                                           SRR5189679.81256.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849384 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-60                AC135084.4         178614-178673       c
            61-517              CJ046590.1         2-458
            518-1451            BC049615.1         488-1421
FEATURES             Location/Qualifiers
     source          1..1451
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="17"
                     /map="17 19.54 cM"
     gene            1..1451
                     /gene="Crisp2"
                     /gene_synonym="CRISP-2; GAPDL5; Tpx-1; Tpx1"
                     /note="cysteine-rich secretory protein 2"
                     /db_xref="GeneID:22024"
                     /db_xref="MGI:MGI:98815"
     exon            1..119
                     /gene="Crisp2"
                     /gene_synonym="CRISP-2; GAPDL5; Tpx-1; Tpx1"
                     /inference="alignment:Splign:2.1.0"
     exon            120..202
                     /gene="Crisp2"
                     /gene_synonym="CRISP-2; GAPDL5; Tpx-1; Tpx1"
                     /inference="alignment:Splign:2.1.0"
     exon            203..234
                     /gene="Crisp2"
                     /gene_synonym="CRISP-2; GAPDL5; Tpx-1; Tpx1"
                     /inference="alignment:Splign:2.1.0"
     exon            235..312
                     /gene="Crisp2"
                     /gene_synonym="CRISP-2; GAPDL5; Tpx-1; Tpx1"
                     /inference="alignment:Splign:2.1.0"
     CDS             244..975
                     /gene="Crisp2"
                     /gene_synonym="CRISP-2; GAPDL5; Tpx-1; Tpx1"
                     /note="testis-specific protein TPX-1; testis specific gene
                     1"
                     /codon_start=1
                     /product="cysteine-rich secretory protein 2 precursor"
                     /protein_id="NP_001191000.1"
                     /db_xref="CCDS:CCDS28786.1"
                     /db_xref="GeneID:22024"
                     /db_xref="MGI:MGI:98815"
                     /translation="MAWFQVMLFVFALLLRSPLTEGKDPDFTSLLTNQLQVQREIVNK
                     HNELRRSVNPTGSDILKMEWSIQATTNAQKWANKCILEHSSKDDRKINIRCGENLYMS
                     TDPTLWSTVIQSWYNENEDFVYGVGAKPNSAVGHYTQLVWYSSFKIGCGIAYCPNQDN
                     LKYFYVCHYCPMGNNVMKKSTPYQQGTPCASCPNNCENGLCTNSCDFEDLLSNCESLK
                     TSAGCKHELLKTKCQATCLCEDKIH"
     exon            313..429
                     /gene="Crisp2"
                     /gene_synonym="CRISP-2; GAPDL5; Tpx-1; Tpx1"
                     /inference="alignment:Splign:2.1.0"
     exon            430..517
                     /gene="Crisp2"
                     /gene_synonym="CRISP-2; GAPDL5; Tpx-1; Tpx1"
                     /inference="alignment:Splign:2.1.0"
     exon            518..660
                     /gene="Crisp2"
                     /gene_synonym="CRISP-2; GAPDL5; Tpx-1; Tpx1"
                     /inference="alignment:Splign:2.1.0"
     exon            661..758
                     /gene="Crisp2"
                     /gene_synonym="CRISP-2; GAPDL5; Tpx-1; Tpx1"
                     /inference="alignment:Splign:2.1.0"
     exon            759..847
                     /gene="Crisp2"
                     /gene_synonym="CRISP-2; GAPDL5; Tpx-1; Tpx1"
                     /inference="alignment:Splign:2.1.0"
     exon            848..1451
                     /gene="Crisp2"
                     /gene_synonym="CRISP-2; GAPDL5; Tpx-1; Tpx1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1429..1434
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Crisp2"
                     /gene_synonym="CRISP-2; GAPDL5; Tpx-1; Tpx1"
                     /note="hexamer: AATAAA"
     polyA_site      1449
                     /gene="Crisp2"
                     /gene_synonym="CRISP-2; GAPDL5; Tpx-1; Tpx1"
                     /note="major polyA site"
ORIGIN      
        1 gtgacaccgc ccccacgtgg cggctggagg cggttatcgt tgctcaccga ggggaggggc
       61 aacaagagcc ctttctggct ccttcctgac agcatgaaga cctgtgctgt tgaaccagcg
      121 acaaagaagg gaaacaccat agccctcaca ctttagaaga ggcatgccct cctgatccta
      181 aaacatcgga aggaaggaca agataaggca gatatttcaa ctgtcaaatc aacactttcg
      241 gccatggctt ggttccaggt aatgctgttt gtctttgcgt tgctgctgcg atcaccactc
      301 acagaaggaa aggatccaga ctttacttct ttgttaacta accaactaca agttcaaaga
      361 gagatcgtaa ataaacacaa tgagctgagg agatcagtta accctactgg cagcgacata
      421 ctaaaaatgg aatggagcat acaagcaaca acaaatgctc aaaagtgggc taataagtgt
      481 attttagaac acagttctaa agatgaccgg aagatcaata taagatgtgg tgagaatctc
      541 tatatgtcaa ctgatcctac cctctggtca actgtaattc aaagctggta caatgaaaat
      601 gaagattttg tttatggtgt aggagctaaa cccaattcag ctgtcggaca ttacactcag
      661 cttgtttggt attcatcttt caaaattgga tgtggaattg cttactgtcc caatcaagac
      721 aacctgaaat atttctatgt ttgccattac tgtcctatgg gtaacaatgt gatgaaaaag
      781 agcaccccat atcaacaagg aacaccttgt gctagttgtc ccaataactg tgaaaatggg
      841 ctgtgcacca atagctgtga ttttgaagat ttacttagta actgtgaatc cttgaagact
      901 tcagcaggct gtaaacatga gttgctcaag acaaagtgcc aggctacttg tttgtgtgaa
      961 gacaaaattc attaacatgc ccagtgtgca gcaggacaga ctatatgagg aaggggtaca
     1021 gacttagttg agactgatgg ggaaaatcta taggagatta gtgacatgtc acatcccaaa
     1081 tgacaaggct tctttccttc ctagattcat acattaatat ctttcagaac agccatttaa
     1141 aaaggttgtc atttagtata acaactttgg aattggacca actttgctgc tttaaatata
     1201 gtgaactgaa tcaagtggag aattttgaaa gttgcaccat atcaggtcat tcacctctgg
     1261 aactttgaaa aaaagagaaa actatttgtg tcctaaatta acctgcaatg gaagaatggg
     1321 ctgtagttac atcaacatca accgacttta tagcgcctgc caggatgaag cttgacatct
     1381 acatgtgtct tctgtcttct tacttaaaca cagataatca cttctttcaa taaagaactc
     1441 aagctaccac a
//
