LOCUS       NP_001157038            1282 aa            linear   ROD 14-FEB-2025
DEFINITION  protein crumbs homolog 2 isoform 1 precursor [Mus musculus].
ACCESSION   NP_001157038 XP_140952
VERSION     NP_001157038.1
DBSOURCE    REFSEQ: accession NM_001163566.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (residues 1 to 1282)
  AUTHORS   Cho,S.H., Kim,J.H. and Kim,S.
  TITLE     Perturbed cell cycle phase-dependent positioning and nuclear
            migration of retinal progenitors along the apico-basal axis
            underlie global retinal disorganization in the LCA8-like mouse
            model
  JOURNAL   Dev Biol 517, 39-54 (2025)
   PUBMED   39284539
REFERENCE   2  (residues 1 to 1282)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (residues 1 to 1282)
  AUTHORS   Grego-Bessa,J., Gomez-Apinaniz,P., Prados,B., Gomez,M.J.,
            MacGrogan,D. and de la Pompa,J.L.
  TITLE     Nrg1 Regulates Cardiomyocyte Migration and Cell Cycle in
            Ventricular Development
  JOURNAL   Circ Res 133 (11), 927-943 (2023)
   PUBMED   37846569
REFERENCE   4  (residues 1 to 1282)
  AUTHORS   Hada,I., Shimizu,A., Takematsu,H., Nishibori,Y., Kimura,T.,
            Fukutomi,T., Kudo,A., Ito-Nitta,N., Kiuchi,Z., Patrakka,J.,
            Mikami,N., Leclerc,S., Akimoto,Y., Hirayama,Y., Mori,S., Takano,T.
            and Yan,K.
  TITLE     A Novel Mouse Model of Idiopathic Nephrotic Syndrome Induced by
            Immunization with the Podocyte Protein Crb2
  JOURNAL   J Am Soc Nephrol 33 (11), 2008-2025 (2022)
   PUBMED   35985815
  REMARK    GeneRIF: A Novel Mouse Model of Idiopathic Nephrotic Syndrome
            Induced by Immunization with the Podocyte Protein Crb2.
REFERENCE   5  (residues 1 to 1282)
  AUTHORS   Tanoue,A., Katayama,K., Ito,Y., Joh,K., Toda,M., Yasuma,T.,
            D'Alessandro-Gabazza,C.N., Kawachi,H., Yan,K., Ito,M.,
            Gabazza,E.C., Tryggvason,K. and Dohi,K.
  TITLE     Podocyte-specific Crb2 knockout mice develop focal segmental
            glomerulosclerosis
  JOURNAL   Sci Rep 11 (1), 20556 (2021)
   PUBMED   34654837
  REMARK    GeneRIF: Podocyte-specific Crb2 knockout mice develop focal
            segmental glomerulosclerosis.
            Publication Status: Online-Only
REFERENCE   6  (residues 1 to 1282)
  AUTHORS   Gosens,I., Sessa,A., den Hollander,A.I., Letteboer,S.J.,
            Belloni,V., Arends,M.L., Le Bivic,A., Cremers,F.P., Broccoli,V. and
            Roepman,R.
  TITLE     FERM protein EPB41L5 is a novel member of the mammalian CRB-MPP5
            polarity complex
  JOURNAL   Exp Cell Res 313 (19), 3959-3970 (2007)
   PUBMED   17920587
REFERENCE   7  (residues 1 to 1282)
  AUTHORS   Lee,J.D., Silva-Gagliardi,N.F., Tepass,U., McGlade,C.J. and
            Anderson,K.V.
  TITLE     The FERM protein Epb4.1l5 is required for organization of the
            neural plate and for the epithelial-mesenchymal transition at the
            primitive streak of the mouse embryo
  JOURNAL   Development 134 (11), 2007-2016 (2007)
   PUBMED   17507402
REFERENCE   8  (residues 1 to 1282)
  AUTHORS   van Rossum,A.G., Aartsen,W.M., Meuleman,J., Klooster,J.,
            Malysheva,A., Versteeg,I., Arsanto,J.P., Le Bivic,A. and
            Wijnholds,J.
  TITLE     Pals1/Mpp5 is required for correct localization of Crb1 at the
            subapical region in polarized Muller glia cells
  JOURNAL   Hum Mol Genet 15 (18), 2659-2672 (2006)
   PUBMED   16885194
REFERENCE   9  (residues 1 to 1282)
  AUTHORS   Augustin,M., Sedlmeier,R., Peters,T., Huffstadt,U., Kochmann,E.,
            Simon,D., Schoniger,M., Garke-Mayerthaler,S., Laufs,J., Mayhaus,M.,
            Franke,S., Klose,M., Graupner,A., Kurzmann,M., Zinser,C., Wolf,A.,
            Voelkel,M., Kellner,M., Kilian,M., Seelig,S., Koppius,A.,
            Teubner,A., Korthaus,D., Nehls,M. and Wattler,S.
  TITLE     Efficient and fast targeted production of murine models based on
            ENU mutagenesis
  JOURNAL   Mamm Genome 16 (6), 405-413 (2005)
   PUBMED   16075367
REFERENCE   10 (residues 1 to 1282)
  AUTHORS   van den Hurk,J.A., Rashbass,P., Roepman,R., Davis,J.,
            Voesenek,K.E., Arends,M.L., Zonneveld,M.N., van Roekel,M.H.,
            Cameron,K., Rohrschneider,K., Heckenlively,J.R., Koenekoop,R.K.,
            Hoyng,C.B., Cremers,F.P. and den Hollander,A.I.
  TITLE     Characterization of the Crumbs homolog 2 (CRB2) gene and analysis
            of its role in retinitis pigmentosa and Leber congenital amaurosis
  JOURNAL   Mol Vis 11, 263-273 (2005)
   PUBMED   15851977
  REMARK    Publication Status: Online-Only
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL805959.11.
            
            On Jul 25, 2009 this sequence version replaced XP_140952.9.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR9219381.294108.1,
                                           SRR9219382.207580.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849382
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..1282
                     /organism="Mus musculus"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 24.19 cM"
     Protein         1..1282
                     /product="protein crumbs homolog 2 isoform 1 precursor"
                     /note="crumbs-like protein 2; protein crumbs homolog 2;
                     crumbs2"
                     /calculated_mol_wt=130760
     sig_peptide     1..35
                     /inference="COORDINATES: ab initio prediction:SignalP:6.0"
                     /calculated_mol_wt=4019
     mat_peptide     36..1282
                     /product="Protein crumbs homolog 2. /evidence=ECO:0000255.
                     /id=PRO_0000055626"
                     /note="propagated from UniProtKB/Swiss-Prot (Q80YA8.3)"
                     /calculated_mol_wt=130760
     Region          <79..110
                     /region_name="EGF_CA"
                     /note="Calcium-binding EGF-like domain, present in a large
                     number of membrane-bound and extracellular (mostly animal)
                     proteins. Many of these proteins require calcium for their
                     biological function and calcium-binding sites have been
                     found to be located at the...; cd00054"
                     /db_xref="CDD:238011"
     Region          112..148
                     /region_name="EGF_CA"
                     /note="Calcium-binding EGF-like domain, present in a large
                     number of membrane-bound and extracellular (mostly animal)
                     proteins. Many of these proteins require calcium for their
                     biological function and calcium-binding sites have been
                     found to be located at the...; cd00054"
                     /db_xref="CDD:238011"
     Site            order(112,115,129)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:238011"
     Region          151..186
                     /region_name="EGF_CA"
                     /note="Calcium-binding EGF-like domain, present in a large
                     number of membrane-bound and extracellular (mostly animal)
                     proteins. Many of these proteins require calcium for their
                     biological function and calcium-binding sites have been
                     found to be located at the...; cd00054"
                     /db_xref="CDD:238011"
     Region          188..225
                     /region_name="EGF_CA"
                     /note="Calcium-binding EGF-like domain, present in a large
                     number of membrane-bound and extracellular (mostly animal)
                     proteins. Many of these proteins require calcium for their
                     biological function and calcium-binding sites have been
                     found to be located at the...; cd00054"
                     /db_xref="CDD:238011"
     Site            order(188,191,205)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:238011"
     Region          230..263
                     /region_name="EGF_CA"
                     /note="Calcium-binding EGF-like domain, present in a large
                     number of membrane-bound and extracellular (mostly animal)
                     proteins. Many of these proteins require calcium for their
                     biological function and calcium-binding sites have been
                     found to be located at the...; cd00054"
                     /db_xref="CDD:238011"
     Site            239
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (Q80YA8.3)"
     Site            271
                     /site_type="glycosylation"
                     /note="O-linked (Glc...) serine.
                     /evidence=ECO:0000305|PubMed:26496195; propagated from
                     UniProtKB/Swiss-Prot (Q80YA8.3)"
     Region          324..360
                     /region_name="EGF_CA"
                     /note="Calcium-binding EGF-like domain, present in a large
                     number of membrane-bound and extracellular (mostly animal)
                     proteins. Many of these proteins require calcium for their
                     biological function and calcium-binding sites have been
                     found to be located at the...; cd00054"
                     /db_xref="CDD:238011"
     Site            order(324,327,341)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:238011"
     Region          362..397
                     /region_name="EGF_CA"
                     /note="Calcium-binding EGF-like domain, present in a large
                     number of membrane-bound and extracellular (mostly animal)
                     proteins. Many of these proteins require calcium for their
                     biological function and calcium-binding sites have been
                     found to be located at the...; cd00054"
                     /db_xref="CDD:238011"
     Site            order(362,365,379)
                     /site_type="other"
                     /note="Ca2+ binding site [ion binding]"
                     /db_xref="CDD:238011"
     Site            442
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (Q80YA8.3)"
     Region          469..592
                     /region_name="Laminin_G_2"
                     /note="Laminin G domain; pfam02210"
                     /db_xref="CDD:460494"
     Region          613..643
                     /region_name="EGF"
                     /note="EGF-like domain; pfam00008"
                     /db_xref="CDD:394967"
     Region          666..776
                     /region_name="LamG"
                     /note="Laminin G domain; Laminin G-like domains are
                     usually Ca++ mediated receptors that can have binding
                     sites for steroids, beta1 integrins, heparin, sulfatides,
                     fibulin-1, and alpha-dystroglycans. Proteins that contain
                     LamG domains serve a variety of...; cd00110"
                     /db_xref="CDD:238058"
     Site            672
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (Q80YA8.3)"
     Site            693
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (Q80YA8.3)"
     Site            789
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (Q80YA8.3)"
     Site            803
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (Q80YA8.3)"
     Region          814..844
                     /region_name="EGF"
                     /note="EGF-like domain; pfam00008"
                     /db_xref="CDD:394967"
     Site            839
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (Q80YA8.3)"
     Region          875..1014
                     /region_name="LamG"
                     /note="Laminin G domain; Laminin G-like domains are
                     usually Ca++ mediated receptors that can have binding
                     sites for steroids, beta1 integrins, heparin, sulfatides,
                     fibulin-1, and alpha-dystroglycans. Proteins that contain
                     LamG domains serve a variety of...; cd00110"
                     /db_xref="CDD:238058"
     Site            889
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (Q80YA8.3)"
     Site            929
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (Q80YA8.3)"
     Site            1006
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (Q80YA8.3)"
     Region          1058..1089
                     /region_name="EGF_CA"
                     /note="Calcium-binding EGF-like domain, present in a large
                     number of membrane-bound and extracellular (mostly animal)
                     proteins. Many of these proteins require calcium for their
                     biological function and calcium-binding sites have been
                     found to be located at the...; cd00054"
                     /db_xref="CDD:238011"
     Site            1138
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (Q80YA8.3)"
     Site            1155
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (Q80YA8.3)"
     Region          1175..1206
                     /region_name="EGF_CA"
                     /note="Calcium-binding EGF-like domain, present in a large
                     number of membrane-bound and extracellular (mostly animal)
                     proteins. Many of these proteins require calcium for their
                     biological function and calcium-binding sites have been
                     found to be located at the...; cd00054"
                     /db_xref="CDD:238011"
     Site            1222..1242
                     /site_type="transmembrane region"
                     /note="propagated from UniProtKB/Swiss-Prot (Q80YA8.3)"
     Region          1246..1282
                     /region_name="Interaction with EPB41L5.
                     /evidence=ECO:0000250|UniProtKB:Q5IJ48"
                     /note="propagated from UniProtKB/Swiss-Prot (Q80YA8.3)"
     CDS             1..1282
                     /gene="Crb2"
                     /gene_synonym="5930402A21"
                     /coded_by="NM_001163566.2:93..3941"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1"
                     /db_xref="CCDS:CCDS50580.1"
                     /db_xref="GeneID:241324"
                     /db_xref="MGI:MGI:2679260"
ORIGIN      
        1 malvgpriwg prrdiyplll llllllllll pwvpaglvpp etpsvcasdp capgtkcqat
       61 esggytceps elggcatqpc hhgalcvpqg pdpnsfrcyc vpgfqgphce ldidecasrp
      121 cqhggtcqnl adhyechcpl gyagvtceae vdecssapcl hggscldgvg syrcvcapgy
      181 agancqldvd ecqsqpcahg gvchdlvngf rcdcadtgye garceqevle casapcahna
      241 scldgfrsfr clcwpgfsge rcevdedeca sgpcqnggqc lqrsdptlyg gvqaifpgaf
      301 sfshaagflc scplgfagnd csmdvdecas gpclnggscq dlpngfqcyc qdgytgltcq
      361 edmdecqsep clhggtcsdt vagyicqcpe awgghdcsvq ltgcqghtcp laatciptfk
      421 sglhgyfcrc ppgtygpfcg qnttfsvvsg ssvwglvpaa aslglalrfr ttllagtlat
      481 lkdtrdslel vlvgavlqat lsrhgtavli ltlpdlalnd ghwhqvevtl hlgtlelrlw
      541 hegcpgqlcv asgpvatgpt asvasgppgs ysiylgggvf agcfqdvrve ghlllpeelk
      601 gtvllgcerr epcqplpcah ggacvdlwth frcdcprpyr gatctdevpa atfglggats
      661 sasfllhqlg pnltvsfflr trepaglllq fandsvaslt vflsegqira eglghpavvl
      721 pgrwddglph lvmlsfgpdq lqdlgqrlyv ggrfypddtq lwggpfrgcl qdlqlnsihl
      781 pffsspmens swpseleagq ssnltqgcvs edtcnpnpcf nggtchvtwn dfyctcsenf
      841 tgptcaqqrw cprqpclppa tceevpdgfv cvaeatfreg ppavftghnv ssslsgltla
      901 frtrdseagl lravsaagah sniwlavrng slagdvagsv lpapgprvad gawhrvrlar
      961 efpqaaasrw llwldgaatp valhglggdl gflqgpgavp lllaenftgc lgrvalgdfp
     1021 lplapprsgt vsgarehfva wpgspavslg crggpvcsps pclhggacrd lfdafacscg
     1081 pawegprcei radpcrstpc vrgqcharpd grfecrcppg fsgprcrlpv lpqgcnlnst
     1141 ckdgapcegg plgtncscqe glaglrcqsl dkpceaspcl nggtcrvasg ifectcsagf
     1201 sgqfcevvkt lplplpfpll evavpaacac llllllglls gilaarkrrq segtyspsqq
     1261 evagarlemd svlkvppeer li
//
