LOCUS       NM_007760               4393 bp    mRNA    linear   ROD 12-FEB-2023
DEFINITION  Mus musculus carnitine acetyltransferase (Crat), mRNA.
ACCESSION   NM_007760
VERSION     NM_007760.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4393)
  AUTHORS   Lasheras-Otero I, Feliu I, Maillo A, Moreno H, Redondo-Munoz M,
            Aldaz P, Bocanegra A, Olias-Arjona A, Lecanda F, Fernandez-Irigoyen
            J, Santamaria E, Larrayoz IM, Gomez-Cabrero D, Wellbrock C, Vicent
            S and Arozarena I.
  TITLE     The Regulators of Peroxisomal Acyl-Carnitine Shuttle CROT and CRAT
            Promote Metastasis in Melanoma
  JOURNAL   J Invest Dermatol 143 (2), 305-316 (2023)
   PUBMED   36058299
  REMARK    GeneRIF: The Regulators of Peroxisomal Acyl-Carnitine Shuttle CROT
            and CRAT Promote Metastasis in Melanoma.
REFERENCE   2  (bases 1 to 4393)
  AUTHORS   Williams AS, Koves TR, Pettway YD, Draper JA, Slentz DH, Grimsrud
            PA, Ilkayeva OR and Muoio DM.
  TITLE     Nicotinamide riboside supplementation confers marginal metabolic
            benefits in obese mice without remodeling the muscle
            acetyl-proteome
  JOURNAL   iScience 25 (1), 103635 (2021)
   PUBMED   35028529
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 4393)
  AUTHORS   Berdous D, Berney X, Sanchez-Archidona AR, Jan M, Roujeau C,
            Lopez-Mejia IC, Mynatt R and Thorens B.
  TITLE     A genetic screen identifies Crat as a regulator of pancreatic
            beta-cell insulin secretion
  JOURNAL   Mol Metab 37, 100993 (2020)
   PUBMED   32298772
REFERENCE   4  (bases 1 to 4393)
  AUTHORS   Williams AS, Koves TR, Davidson MT, Crown SB, Fisher-Wellman KH,
            Torres MJ, Draper JA, Narowski TM, Slentz DH, Lantier L, Wasserman
            DH, Grimsrud PA and Muoio DM.
  TITLE     Disruption of Acetyl-Lysine Turnover in Muscle Mitochondria
            Promotes Insulin Resistance and Redox Stress without Overt
            Respiratory Dysfunction
  JOURNAL   Cell Metab 31 (1), 131-147 (2020)
   PUBMED   31813822
REFERENCE   5  (bases 1 to 4393)
  AUTHORS   Kruger C, Nguyen TT, Breaux C, Guillory A, Mangelli M, Fridianto
            KT, Kovalik JP, Burk DH, Noland RC, Mynatt R and Stadler K.
  TITLE     Proximal Tubular Cell-Specific Ablation of Carnitine
            Acetyltransferase Causes Tubular Disease and Secondary
            Glomerulosclerosis
  JOURNAL   Diabetes 68 (4), 819-831 (2019)
   PUBMED   30728184
  REMARK    GeneRIF: we modeled mitochondrial overload by creating mice lacking
            the enzyme carnitine acetyltransferase (CrAT) in the proximal
            tubules, thus limiting a primary mechanism to export carbons under
            conditions of substrate excess. Mice developed tubular disease and,
            interestingly, secondary glomerulosclerosis. This was accompanied
            by increased levels of apoptosis, fibrosis and oxidative stress.
REFERENCE   6  (bases 1 to 4393)
  AUTHORS   Morillas M, Lopez-Vinas E, Valencia A, Serra D, Gomez-Puertas P,
            Hegardt FG and Asins G.
  TITLE     Structural model of carnitine palmitoyltransferase I based on the
            carnitine acetyltransferase crystal
  JOURNAL   Biochem J 379 (Pt 3), 777-784 (2004)
   PUBMED   14711372
  REMARK    GeneRIF: structural model for L-CPT I (liver CPT I), based on the
            similarity of this enzyme to the recently crystallized mouse
            carnitine acetyltransferase
REFERENCE   7  (bases 1 to 4393)
  AUTHORS   Mootha VK, Bunkenborg J, Olsen JV, Hjerrild M, Wisniewski JR, Stahl
            E, Bolouri MS, Ray HN, Sihag S, Kamal M, Patterson N, Lander ES and
            Mann M.
  TITLE     Integrated analysis of protein composition, tissue diversity, and
            gene regulation in mouse mitochondria
  JOURNAL   Cell 115 (5), 629-640 (2003)
   PUBMED   14651853
REFERENCE   8  (bases 1 to 4393)
  AUTHORS   Jogl G and Tong L.
  TITLE     Crystal structure of carnitine acetyltransferase and implications
            for the catalytic mechanism and fatty acid transport
  JOURNAL   Cell 112 (1), 113-122 (2003)
   PUBMED   12526798
REFERENCE   9  (bases 1 to 4393)
  AUTHORS   Cox KB, Johnson KR and Wood PA.
  TITLE     Chromosomal locations of the mouse fatty acid oxidation genes
            Cpt1a, Cpt1b, Cpt2, Acadvl, and metabolically related Crat gene
  JOURNAL   Mamm Genome 9 (8), 608-610 (1998)
   PUBMED   9680378
REFERENCE   10 (bases 1 to 4393)
  AUTHORS   Brunner S, Kramar K, Denhardt DT and Hofbauer R.
  TITLE     Cloning and characterization of murine carnitine acetyltransferase:
            evidence for a requirement during cell cycle progression
  JOURNAL   Biochem J 322 (Pt 2) (Pt 2), 403-410 (1997)
   PUBMED   9065756
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL954299.13, AK041431.1 and AK132179.1.
            
            [WARNING] On Apr 25, 2023 this sequence was replaced by
            NM_007760.4.
            
            On Jan 31, 2006 this sequence version replaced NM_007760.2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660815.265131.1, AK041431.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849381, SAMN00849382
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            RefSeq Select criteria             :: based on conservation,
                                                  expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-227               AL954299.13        9722-9948           c
            228-464             AK041431.1         2-238
            465-4393            AK132179.1         292-4220
FEATURES             Location/Qualifiers
     source          1..4393
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 21.69 cM"
     gene            1..4393
                     /gene="Crat"
                     /gene_synonym="CARAT"
                     /note="carnitine acetyltransferase"
                     /db_xref="GeneID:12908"
                     /db_xref="MGI:MGI:109501"
     exon            1..437
                     /gene="Crat"
                     /gene_synonym="CARAT"
                     /inference="alignment:Splign:2.1.0"
     CDS             411..2291
                     /gene="Crat"
                     /gene_synonym="CARAT"
                     /EC_number="2.3.1.7"
                     /EC_number="2.3.1.137"
                     /note="CAT; carnitine acetylase"
                     /codon_start=1
                     /product="carnitine O-acetyltransferase"
                     /protein_id="NP_031786.2"
                     /db_xref="CCDS:CCDS15882.1"
                     /db_xref="GeneID:12908"
                     /db_xref="MGI:MGI:109501"
                     /translation="MLAFAARTVVKPLGLLKPSSLMKVSGRFKAHQDALPRLPVPPLQ
                     QSLDYYLKALQPIVSEEEWAHTKQLVDEFQTSGGVGERLQKGLERRAKKMENWLSEWW
                     LKTAYLQFRQPVVIYSSPGVILPKQDFVDLQGQLRFAAKLIEGVLDFKSMIDNETLPV
                     EFLGGQPLCMNQYYQILSSCRVPGPKQDSVVNFLKSKRPPTHITVVHNYQFFELDVYH
                     SDGTPLTSDQIFVQLEKIWNSSLQSNKEPVGILTSNHRNTWAKAYNNLIKDKVNRESV
                     NSIQKSIFTVCLDKQVPRVSDDVYRNHVAGQMLHGGGSKFNSGNRWFDKTLQFIVAED
                     GSCGMVYEHAAAEGPPIVALVDHVMEYTKKPELVRSPMVPLPMPKKLRFNITPEIKND
                     IEKAKQNLSIMIQDLDIMMLTFHHFGKDFPKSEKLSPDAFIQVALQLAYYRIYGQACA
                     TYESASLRMFHLGRTDTIRSASIDSLAFVKGMGDSTVPEQQKVELLRKAVQAHRAYTD
                     RAIRGEAFDRHLLGLKLQAIEDLVSMPDIFMDTSYAIAMHFNLSTSQVPAKTDCVMFF
                     GPVVPDGYGICYNPMEAHINFSVSAYNSCAETNAARMAHYLEKALLDMRTLLQNHPRA
                     KL"
     exon            438..701
                     /gene="Crat"
                     /gene_synonym="CARAT"
                     /inference="alignment:Splign:2.1.0"
     exon            702..820
                     /gene="Crat"
                     /gene_synonym="CARAT"
                     /inference="alignment:Splign:2.1.0"
     exon            821..874
                     /gene="Crat"
                     /gene_synonym="CARAT"
                     /inference="alignment:Splign:2.1.0"
     exon            875..1040
                     /gene="Crat"
                     /gene_synonym="CARAT"
                     /inference="alignment:Splign:2.1.0"
     exon            1041..1215
                     /gene="Crat"
                     /gene_synonym="CARAT"
                     /inference="alignment:Splign:2.1.0"
     exon            1216..1394
                     /gene="Crat"
                     /gene_synonym="CARAT"
                     /inference="alignment:Splign:2.1.0"
     exon            1395..1495
                     /gene="Crat"
                     /gene_synonym="CARAT"
                     /inference="alignment:Splign:2.1.0"
     exon            1496..1615
                     /gene="Crat"
                     /gene_synonym="CARAT"
                     /inference="alignment:Splign:2.1.0"
     exon            1616..1738
                     /gene="Crat"
                     /gene_synonym="CARAT"
                     /inference="alignment:Splign:2.1.0"
     exon            1739..1874
                     /gene="Crat"
                     /gene_synonym="CARAT"
                     /inference="alignment:Splign:2.1.0"
     exon            1875..1937
                     /gene="Crat"
                     /gene_synonym="CARAT"
                     /inference="alignment:Splign:2.1.0"
     exon            1938..2075
                     /gene="Crat"
                     /gene_synonym="CARAT"
                     /inference="alignment:Splign:2.1.0"
     exon            2076..4393
                     /gene="Crat"
                     /gene_synonym="CARAT"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 tcgacagcta aggtggcttt cggaggcgga gagcacgagc gggacagcgc ctaccgttgt
       61 gaccccgtga cgggagccca gcgccgggct gctaacagct gagctagagg tccgtctccg
      121 caagccccgc cctctgcggc cactccccgg cttgggccca ccccgagatc ggggcgagcg
      181 gccggggact gcgagccacc aaagctccgc cccgtgagtc ctgccccacc ggcagcccgc
      241 cgggcccggt ggccgcgggg ccgcctctac tggctagcgg cagtgactcg gcgaccttga
      301 acctagaacc ggagccgcga acccactgac cccagcaccc cagcagtggc gcagtcttta
      361 cccagcgcga ggggcggagc ggaccaagtg tccgcaaaca gactgggacg atgttagcct
      421 ttgctgccag aaccgtggta aaacccctgg gcctcctcaa gccctcctcc ttgatgaagg
      481 tttctggtcg cttcaaggcc caccaagatg cactgccaag gctgcctgtg cccccacttc
      541 agcaatccct ggactattac ctcaaggcac tgcagcccat cgtgagtgag gaagaatggg
      601 ctcacaccaa gcaactggtg gatgagtttc agacctcagg gggcgtaggg gaacgcctac
      661 agaagggact ggagcgcagg gccaagaaaa tggagaactg gctgtccgag tggtggctca
      721 agacagccta tctccagttc cgccagcctg tggtcatcta ctccagccca ggagtgatcc
      781 tgcccaagca ggactttgtg gatctacagg gtcagcttcg gtttgctgcc aaactcatcg
      841 agggtgtatt ggatttcaag tccatgattg acaatgagac gctgccagtt gagttcctgg
      901 gaggacagcc gctgtgcatg aaccagtatt atcagatcct gtcttcttgc cgtgtgcccg
      961 gccccaagca ggactcagtg gtgaacttct taaagtcaaa gagaccaccc acgcatataa
     1021 ccgtggtgca taactaccag ttcttcgagc tggatgtgta ccacagtgac ggaacaccgc
     1081 tcacctcaga tcagatcttt gtgcagctag agaaaatatg gaattcctca ctgcaatcca
     1141 acaaagagcc tgttggcatc ctaacctcca accaccgaaa cacctgggcc aaggcctaca
     1201 acaacctcat taaagacaaa gttaaccgag agtcagtgaa ctccatacag aagagcatct
     1261 tcaccgtgtg cctggacaag caagtgccta gagtctcaga tgatgtctac cgaaaccacg
     1321 tagcaggcca gatgctacat ggtggtggta gcaagttcaa cagtggcaac cgctggtttg
     1381 acaagacact gcagttcatc gtggcagaag acggctcctg tgggatggtg tatgagcatg
     1441 cagctgcaga agggcccccc atagttgcac ttgtggacca tgtcatggag tatacaaaaa
     1501 agcctgagct tgtgcggtct cctatggtgc ccttgccaat gcccaagaag ctgcggttca
     1561 acatcacacc tgagatcaag aacgacatag agaaagccaa acagaacctc agcatcatga
     1621 tccaggacct ggacatcatg atgctgacgt tccatcactt tggaaaggac ttccccaagt
     1681 ccgagaagct aagccctgat gccttcatcc aggtagccct gcagctggca tactacagga
     1741 tctatgggca ggcgtgtgcc acgtatgaaa gtgcctctct gcgcatgttt cacctgggcc
     1801 gcactgacac catccgctca gcctccatag actcgctggc ctttgtcaaa ggcatgggtg
     1861 actccacagt gccggagcag cagaaggtgg agctgctccg gaaggctgtg caggcccacc
     1921 gagcctacac tgaccgggcc atccgagggg aggcctttga ccggcacctg ctgggtctga
     1981 agctgcaggc catcgaggac ctggtcagca tgcctgacat cttcatggac acctcttacg
     2041 ccattgctat gcacttcaac ctctctacca gccaggtccc tgccaagaca gactgtgtca
     2101 tgttcttcgg acctgtggtc ccagatggtt atggcatctg ctacaacccc atggaggccc
     2161 atatcaactt ctccgtgtca gcctacaaca gctgtgctga gaccaacgct gcccgcatgg
     2221 ctcactactt ggagaaagct ctgctggaca tgcgcaccct actccagaac caccccaggg
     2281 ccaagctctg agcccaggcc cagacctgcc acttccacag ccaaacccac tttgggatgg
     2341 acacttgcca tggctcactt ccttggctcc cgtgccctcc agtttgagcc agaaaagcat
     2401 tctctagcaa ggccccaacg tccacaccaa gtgccttcca ggccctgtcc ttagcacctg
     2461 ccggagtcca tgtgcagggc tgagtccaga ggctgaacaa gactacggcc caggttccca
     2521 atgtatcatc cactccatga accatggaga agagagccag tccagcccat cccttctcac
     2581 agcaggagcc gggaagagtt cctttcctca gcctggccaa tctcttgtca tctccacagc
     2641 caggccaaaa tctgtcttca ttggtaaatg cccaaggacc agcggtggga ggattatcta
     2701 tcagtgaggg ctctcaacct ccccatagct aagggttaag tctttgtagc caaagggaca
     2761 tctgaggccc agcctccacc agcccacctc aaggtgccat ctccctgctg ccacactctg
     2821 gtgactcttg agccccagac ttattgcttc cattcagtgt gattcttgct gcagtcatgg
     2881 tggtataatg tgattatgcc aggtgatcaa taaagggaag aagtgctggt gaccttgatt
     2941 cagctgaggg gcaagcaggg cagatgtctg gccctgccat tttctacctg tctctggttc
     3001 agggagcttg tatccaagca ggcagtgtgg gccagcaagc tcccagagac aacatcctca
     3061 ggctgagctg tgacaactaa ggcattgagc caaggatgtc gccaaaccct aatgagccac
     3121 cttcctgtcc tggattaggc cttcccaagt cccatggtta gtgcagtgct atggatctgg
     3181 ggtggcaaac actcctcagc ccaaagccag gtgcagctgg cctccactga actcaatagg
     3241 aggggcaatg gagcagggac acactagcag cccagtcaat acaaacagat gctgactgga
     3301 agtgggctgg aggagctgtc actatcaggt ccccaacctt gacacctgct agagggtaca
     3361 tgtcctgcgg aagcccctgg gtacatatgt gcttggaatg tggggccggg aggctggcac
     3421 ccctgtgaag tgctccgtga cctgactgac ctgtggtcac ctgaggcagg gcctgcaaca
     3481 taggatgaaa ctacctcaga gaagctaacg tcactgctac taaatgacag gctcccaaga
     3541 gtacattagt catctgtaag gcagggaagg gcttgagact ctgaagctca gctcacccag
     3601 gctcacacca catccctcag ccatcccagg gcagcaagta tacagaaagg aactgtctag
     3661 tcagggtgct agcaggggct aaccagattt ccccaggatg tatcctagtt ctgaccaggc
     3721 cacagaaaag acctgaactc cactgtaact aaaaaactgt tagtaatttg aagtctagaa
     3781 tacattcttt ctcctggggg tcactactag ttagaagtta taatccaact gcttcagcag
     3841 gcttcccaca tgtaagtgtg tatctcccat atactcccac ccagagggtg tagcacataa
     3901 cctgccagga aaggaaagcc agcctgtgcg cactcagtgc tgcatggcct ctggtcatca
     3961 gcttggtggc gggcagttct tctaaggcag aagtctcctc actgaggcag acaatgcatg
     4021 tggagttgga tatggaagcc ttgtgaaggg cagacctccc caggcctcag gactattctg
     4081 tggaggaaaa cctggccgga ggcaggctgg cagggaggga gggtttttga cagtagcaca
     4141 gaccagacat agcggcatag ctatgtacca gagtgtacca gaggctggca ggcagtggca
     4201 gatgttgggg tcattcaaat cctcactttg gggggctaaa gggaacaaaa atatcctgga
     4261 aatgcctgtg acttcatggt cttaccacat ccttgtctct gagcctcctt tcagaagctc
     4321 aagtaatgta tgctcagcct ccagagtttc agattttatt ttaaaacaat agtaaacaat
     4381 ttcatttgat ttc
//
