LOCUS       NM_001368877            7613 bp    mRNA    linear   ROD 09-FEB-2025
DEFINITION  Mus musculus calcium release activated channel regulator 2A
            (Cracr2a), transcript variant 1, mRNA.
ACCESSION   NM_001368877 XM_006506344
VERSION     NM_001368877.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 7613)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 7613)
  AUTHORS   Pedicini,L., Smith,J., Savic,S. and McKeown,L.
  TITLE     Rab46: a novel player in mast cell function
  JOURNAL   Discov Immunol 3 (1), kyad028 (2023)
   PUBMED   38567292
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 7613)
  AUTHORS   Woo,J.S., Srikanth,S., Kim,K.D., Elsaesser,H., Lu,J.,
            Pellegrini,M., Brooks,D.G., Sun,Z. and Gwack,Y.
  TITLE     CRACR2A-Mediated TCR Signaling Promotes Local Effector Th1 and Th17
            Responses
  JOURNAL   J Immunol 201 (4), 1174-1185 (2018)
   PUBMED   29987160
  REMARK    GeneRIF: this study demonstrates the requirement of
            CRACR2A-mediated TCR signaling in Th1 responses as well as
            pathogenic conversion of Th17 cells, which occurs at the site of
            inflammation
            Erratum:[J Immunol. 2019 Jul 1;203(1):293. doi:
            10.4049/jimmunol.1900492.. PMID: 31127031]
REFERENCE   4  (bases 1 to 7613)
  AUTHORS   Srikanth,S., Kim,K.D., Gao,Y., Woo,J.S., Ghosh,S., Calmettes,G.,
            Paz,A., Abramson,J., Jiang,M. and Gwack,Y.
  TITLE     A large Rab GTPase encoded by CRACR2A is a component of subsynaptic
            vesicles that transmit T cell activation signals
  JOURNAL   Sci Signal 9 (420), ra31 (2016)
   PUBMED   27016526
  REMARK    GeneRIF: GTP binding and prenylation of CRACR2A were associated
            with its localization near the Golgi and its stability
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 7613)
  AUTHORS   Koscielny,G., Yaikhom,G., Iyer,V., Meehan,T.F., Morgan,H.,
            Atienza-Herrero,J., Blake,A., Chen,C.K., Easty,R., Di Fenza,A.,
            Fiegel,T., Grifiths,M., Horne,A., Karp,N.A., Kurbatova,N.,
            Mason,J.C., Matthews,P., Oakley,D.J., Qazi,A., Regnart,J.,
            Retha,A., Santos,L.A., Sneddon,D.J., Warren,J., Westerberg,H.,
            Wilson,R.J., Melvin,D.G., Smedley,D., Brown,S.D., Flicek,P.,
            Skarnes,W.C., Mallon,A.M. and Parkinson,H.
  TITLE     The International Mouse Phenotyping Consortium Web Portal, a
            unified point of access for knockout mice and related phenotyping
            data
  JOURNAL   Nucleic Acids Res 42 (Database issue), D802-D809 (2014)
   PUBMED   24194600
REFERENCE   6  (bases 1 to 7613)
  AUTHORS   Srikanth,S., Jung,H.J., Kim,K.D., Souda,P., Whitelegge,J. and
            Gwack,Y.
  TITLE     A novel EF-hand protein, CRACR2A, is a cytosolic Ca2+ sensor that
            stabilizes CRAC channels in T cells
  JOURNAL   Nat Cell Biol 12 (5), 436-446 (2010)
   PUBMED   20418871
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC173480.1 and AC127373.3.
            
            On Mar 6, 2019 this sequence version replaced XM_006506344.2.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            RNAseq introns :: single sample supports all introns SAMN00849380,
                              SAMN00849383 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            inferred exon combination :: based on alignments, homology
            RefSeq Select criteria    :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-86                AC173480.1         111508-111593
            87-292              AC173480.1         128058-128263
            293-535             AC173480.1         154002-154244
            536-647             AC173480.1         157362-157473
            648-831             AC173480.1         158735-158918
            832-978             AC173480.1         161558-161704
            979-1069            AC173480.1         176007-176097
            1070-1165           AC173480.1         179911-180006
            1166-1353           AC173480.1         181972-182159
            1354-1425           AC127373.3         75071-75142
            1426-1471           AC127373.3         77702-77747
            1472-1578           AC127373.3         79745-79851
            1579-1912           AC127373.3         82988-83321
            1913-2045           AC127373.3         88383-88515
            2046-2127           AC127373.3         96182-96263
            2128-2242           AC127373.3         96348-96462
            2243-2344           AC127373.3         103595-103696
            2345-2421           AC127373.3         106124-106200
            2422-7613           AC127373.3         107581-112772
FEATURES             Location/Qualifiers
     source          1..7613
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="6"
                     /map="6 62.39 cM"
     gene            1..7613
                     /gene="Cracr2a"
                     /gene_synonym="Efcab4b; Gm1073; Gm462"
                     /note="calcium release activated channel regulator 2A"
                     /db_xref="GeneID:381812"
                     /db_xref="MGI:MGI:2685919"
     exon            1..86
                     /gene="Cracr2a"
                     /gene_synonym="Efcab4b; Gm1073; Gm462"
                     /inference="alignment:Splign:2.1.0"
     exon            87..292
                     /gene="Cracr2a"
                     /gene_synonym="Efcab4b; Gm1073; Gm462"
                     /inference="alignment:Splign:2.1.0"
     exon            293..535
                     /gene="Cracr2a"
                     /gene_synonym="Efcab4b; Gm1073; Gm462"
                     /inference="alignment:Splign:2.1.0"
     CDS             326..2506
                     /gene="Cracr2a"
                     /gene_synonym="Efcab4b; Gm1073; Gm462"
                     /note="isoform 1 is encoded by transcript variant 1;
                     EF-hand calcium-binding domain-containing protein 4B;
                     EF-hand calcium binding domain 4B; ras-related protein
                     Rab-46"
                     /codon_start=1
                     /product="EF-hand calcium-binding domain-containing
                     protein 4B isoform 1"
                     /protein_id="NP_001355806.1"
                     /db_xref="CCDS:CCDS90125.1"
                     /db_xref="GeneID:381812"
                     /db_xref="MGI:MGI:2685919"
                     /translation="MATPSGREDSSSQTPGHGKQGSGACVEQLDHPEKLEVEMPDQSA
                     MWKKAQEFFQTCDSEGKGFIARTDMQRLHQELPLSLEELEDVFDALDADGNGFLTPEE
                     FTTGFSHFFFSQNIQGEEEADQQVAQLQEEKVYQSRGEEDVGDMDHDEEAQFQMLMDR
                     LGAQKVLEDESDVRQLWLQLRKDEPHLLSNFEDLLTTIFAQLQEAHEQKNELECALRK
                     KIAAYDEEIQHLYEEMEQQIKSEREQFLLKDTERFQARSRELEKKLSAKEQELERLNQ
                     KQRKLEGQCAALHNDKHETKAENSKLRLTNQELARELERTSHQLQEAQQQLESLQREA
                     CELQQEKEMEVYRVTESLQREKSGLLKQLDFLRERNKHLRDERDITFQKDKAAKANTA
                     ASKASRKQRSGSVIGKYVDGRGILRSQSEEEEDVFSAPRRRSSVGLSAYLQAEEDLGT
                     GEPGSGVPRRQALRRIISIEEDPLPQLLEGGFEKPLSRCPEEEEVSDQGTERQSVAAP
                     VLDLIPTSPRGQPVGKETLYKEERVPSTPDRLFKIVFVGDSAVGKTSFLRRLCEARFS
                     PGMAATVGIDYRVKTVTVDNAQVALQLWDTAGQERYRCISQQFFRKADGVAVMYDLTA
                     KQSFLSIRQWLSSVEEAVGDRIPVLLLGNKLDNEKEREVPRGLGEQLAKENNLIFYEC
                     SACSGHNAQESLLHLARLLKEQEDTVRNDTIQVGPPAKKKSCCG"
     exon            536..647
                     /gene="Cracr2a"
                     /gene_synonym="Efcab4b; Gm1073; Gm462"
                     /inference="alignment:Splign:2.1.0"
     exon            648..831
                     /gene="Cracr2a"
                     /gene_synonym="Efcab4b; Gm1073; Gm462"
                     /inference="alignment:Splign:2.1.0"
     exon            832..978
                     /gene="Cracr2a"
                     /gene_synonym="Efcab4b; Gm1073; Gm462"
                     /inference="alignment:Splign:2.1.0"
     exon            979..1069
                     /gene="Cracr2a"
                     /gene_synonym="Efcab4b; Gm1073; Gm462"
                     /inference="alignment:Splign:2.1.0"
     exon            1070..1165
                     /gene="Cracr2a"
                     /gene_synonym="Efcab4b; Gm1073; Gm462"
                     /inference="alignment:Splign:2.1.0"
     exon            1166..1353
                     /gene="Cracr2a"
                     /gene_synonym="Efcab4b; Gm1073; Gm462"
                     /inference="alignment:Splign:2.1.0"
     exon            1354..1425
                     /gene="Cracr2a"
                     /gene_synonym="Efcab4b; Gm1073; Gm462"
                     /inference="alignment:Splign:2.1.0"
     exon            1426..1471
                     /gene="Cracr2a"
                     /gene_synonym="Efcab4b; Gm1073; Gm462"
                     /inference="alignment:Splign:2.1.0"
     exon            1472..1578
                     /gene="Cracr2a"
                     /gene_synonym="Efcab4b; Gm1073; Gm462"
                     /inference="alignment:Splign:2.1.0"
     exon            1579..1912
                     /gene="Cracr2a"
                     /gene_synonym="Efcab4b; Gm1073; Gm462"
                     /inference="alignment:Splign:2.1.0"
     exon            1913..2045
                     /gene="Cracr2a"
                     /gene_synonym="Efcab4b; Gm1073; Gm462"
                     /inference="alignment:Splign:2.1.0"
     exon            2046..2127
                     /gene="Cracr2a"
                     /gene_synonym="Efcab4b; Gm1073; Gm462"
                     /inference="alignment:Splign:2.1.0"
     exon            2128..2242
                     /gene="Cracr2a"
                     /gene_synonym="Efcab4b; Gm1073; Gm462"
                     /inference="alignment:Splign:2.1.0"
     exon            2243..2344
                     /gene="Cracr2a"
                     /gene_synonym="Efcab4b; Gm1073; Gm462"
                     /inference="alignment:Splign:2.1.0"
     exon            2345..2421
                     /gene="Cracr2a"
                     /gene_synonym="Efcab4b; Gm1073; Gm462"
                     /inference="alignment:Splign:2.1.0"
     exon            2422..7613
                     /gene="Cracr2a"
                     /gene_synonym="Efcab4b; Gm1073; Gm462"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3516..3521
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Cracr2a"
                     /gene_synonym="Efcab4b; Gm1073; Gm462"
                     /note="hexamer: ATTATA"
     polyA_site      3540
                     /gene="Cracr2a"
                     /gene_synonym="Efcab4b; Gm1073; Gm462"
                     /note="major polyA site"
     regulatory      7090..7095
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Cracr2a"
                     /gene_synonym="Efcab4b; Gm1073; Gm462"
                     /note="hexamer: AATAAA"
     polyA_site      7116
                     /gene="Cracr2a"
                     /gene_synonym="Efcab4b; Gm1073; Gm462"
ORIGIN      
        1 agatccggct ggccggcgaa ttcctgacag ctgcgagtca gaaggcaggg ggacaactcg
       61 gacagccgca ctggacgccc ctgcagtgta ctgacatcag atgcccttca ggaggctggc
      121 tgcttatcaa ggcgggacag cagtgacctg ctgctcttct tccgtgagcc caacctggac
      181 atctggtgaa cacttgactc tgccacgtct ccccatgtgg gagcacctct gaaggaccct
      241 gggagaacca gagttcagaa ccctccggat caggagtcat agttgagtcc aggttctgaa
      301 aataacaaag aaatccacac gtccgatggc cacccccagt ggaagagaag actccagctc
      361 ccagacacca ggtcatggga agcagggaag tggagcctgc gtggagcaac tggaccaccc
      421 ggagaagctg gaggtggaga tgccagacca gagtgccatg tggaagaaag cacaggaatt
      481 cttccagacc tgtgactccg agggcaaggg cttcattgcc aggacagaca tgcagaggct
      541 gcatcaggag ttgcccctaa gtctagaaga attggaagat gtgtttgacg ccctggatgc
      601 tgatggcaat ggctttctga ccccagaaga gttcaccact ggatttagtc acttcttctt
      661 cagtcaaaat atccaaggag aggaagaagc tgaccagcag gtggcccagc tccaagagga
      721 gaaagtgtat cagtctagag gggaggagga tgtgggagac atggaccatg atgaagaagc
      781 acaatttcag atgctgatgg acagacttgg agcccagaaa gttctggaag atgaaagtga
      841 cgtcaggcag ctgtggctgc agctgaggaa ggacgagccc cacctgctgt ccaacttcga
      901 agacctcctc accacaattt ttgctcagct ccaagaagcc catgagcaga agaacgaact
      961 ggaatgtgcc ctcagaaaga aaattgctgc ttacgatgaa gaaatccagc atctctatga
     1021 ggagatggag cagcagatca aaagtgagag ggagcagttc ctcctgaaag acacagagag
     1081 attccaggct cgcagccggg agttggaaaa gaagctgagc gccaaggagc aggagctgga
     1141 gcgactcaat cagaagcaga ggaagctgga aggccagtgt gcagctctac acaacgacaa
     1201 gcacgaaacc aaggctgaga acagcaagct gagactcact aaccaggagc tggcccggga
     1261 gttggagcgc acctcccacc aactccagga ggcccagcag cagctggaga gcctccagag
     1321 agaggcctgc gagctgcagc aggagaagga gatggaagtg taccgagtga ctgaaagcct
     1381 gcagcgggag aagtctggcc tcctgaaaca gctggatttc ctaagggaaa ggaacaaaca
     1441 tctccgggac gaacgggaca taactttcca gaaagataag gcggccaagg caaacacagc
     1501 tgcttccaag gcaagcagga agcaaagatc tggctctgtg atcggcaaat acgtggatgg
     1561 caggggaatt ctcaggagcc agtcagagga ggaggaagac gtattcagcg cccccaggag
     1621 gagaagctca gtgggtctga gtgcatatct ccaggcagaa gaggacctgg gaactgggga
     1681 gccagggtct ggtgttccac gtcgccaggc actccgaagg atcatctcca ttgaggagga
     1741 tcccctgcct cagctcctgg aaggaggctt tgagaagcca ctgagcagat gcccagaaga
     1801 ggaggaggtc tctgaccagg gaacagaaag acaaagcgtg gcagccccag tcttggatct
     1861 catccctacc tctccccgag ggcagcctgt tggaaaagag accctctaca aggaggaaag
     1921 ggtcccctct acccctgacc ggctctttaa gatcgtgttt gtgggtgact ctgctgtagg
     1981 gaagacatcc ttcctgagga gactctgtga ggcccgcttc tccccaggca tggcggccac
     2041 tgtgggcatc gactaccgag tgaagacagt cactgtggac aacgcacagg tggccctgca
     2101 gctgtgggat actgctgggc aggagagata tcgctgtatc agtcagcagt tcttcagaaa
     2161 ggccgacggt gtggctgtta tgtatgacct cacagccaag cagtccttcc tgtcgatccg
     2221 ccagtggctc agcagcgtgg aggaagctgt gggagaccga attcctgttc tgctgctggg
     2281 caataaactt gacaatgaga aggagcggga agtgccccgg ggcctgggag agcaactcgc
     2341 caaggagaac aatttgatct tctatgaatg cagtgcctgt tcgggtcaca acgcccaaga
     2401 gtccctgctc cacctggcca ggttactcaa ggagcaagag gacacagtga gaaacgacac
     2461 cattcaggtt ggcccccctg cgaagaagaa atcctgttgt ggctgacagt agtctcccac
     2521 ccccagccag cctgcccatg cccacggtca ccttcagcca gcagcccttc aaagcccaag
     2581 gtcacgtggt tgcttgaatc agcacttggg ttcctatgca gcaacacctg gcaaggccca
     2641 ctctgacttg cccttgttgg tgacttgcct cccctaatgg aaggaagcac ccgcagccag
     2701 gaagactgct ttttctgtat cttgaaatct gttccccttt ctcttctaca ccccaatacc
     2761 cacttctttc cccccccctc caatgtggtt ttctgaaaga aaggagcaga tgggcttttc
     2821 cagaaaagtc accacgtgcc ttctccacct cccttctcct ctctccatgc cctcaggagg
     2881 ctctgcgctg tggttgccat ggcaacagtg caggggcaac ttgctgcttc ttgaaggcca
     2941 aagatgtgcc atttgggtga tgctggggtg gcctgtcttg ctttggcccc tgttctccag
     3001 aaaccagaga gctgctttca gcacatgggt catggacctt gagacactcg aatgtctgct
     3061 ccagctccag gggctgtctg cttctccctg aggttccgaa tcacaagctt caccagcctc
     3121 tcccagctaa cagtgcactc actggatctg cacccgcggg tctcttcagt gtgcttccga
     3181 atgaccctgc agccctggct ccacggtgaa ccatggatga cggctccaca cctggagtta
     3241 cacaacacac atacttccta cttaactttg aaaaggacaa acaaaagggg aagccatcta
     3301 tggctaacga tagtggccat ttctgaaggg tctaaataag atgtatgctg cacataaaaa
     3361 ccactttctg agggtttctc caaatacctc tttggaaaat cacatctgat cattgacagg
     3421 gtagattgac aggcgtgtgt gtggagggga gtggcgtcca acactaagtc tgtaattatt
     3481 ggcttcattt gtttcatttt cttttattcc aggagattat actgattttt tttaaatgta
     3541 gctgtgattt tgtgggtgct tgagaagtga gctgcctcta gctgctgact tcagcagagg
     3601 gcgctaggct tcagggaatg acctgagccc tgcaggggtc cattccttgc ttctggtcag
     3661 cactaaatgt ctgggtgcta gaaaatacat ccttgtccta gatgggaggt aggatagaaa
     3721 gatgacacag ggtgcttttc acaagtgatt gtcattccgc tgacactgtc ttcttcagca
     3781 caagagtcag gcaccaaccc acacccacca agtgaggctc actgagagga cagaatctta
     3841 aggacaaact ccccgtggcc acgtaagcga ggagaaacag caagctagag ttcctagaca
     3901 tgttgcacag cctcttcccc agacccctca aagaacaaga gccccacggt catcaggcac
     3961 ctgactgtgc ctgactttgg aaggctgcat ggaaggaagc acatgactgc tttcgggagg
     4021 cttttctcct tttttgatgt aacatttgca gggagcaaag agccggtggc aaatgcttaa
     4081 cagaacccat acccaggtgc ctcgagaagg tccgtaatgg ccacaggctg gcttccaccc
     4141 cagaccccta tccccatcag ttaaaggtgg ggtgagttct tggatggttg gcagctcttt
     4201 gcttggatgc atagggtggc ttgtgatgtc tctgatgaca cactcacttt tctcacaagc
     4261 tggccccaca cctcaggctc atatctttcc ctagggtaag gacaatggtg gtggttatat
     4321 ttgtggattg gggcatgcgg aagaagcacg agagccactc agaagtggta ctcactgtgt
     4381 gtgtacgtgt gtgtttgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt
     4441 gtgtgtaggg caggtacaag gtaggacggc cacgaacagt gtggccagct cctctggttc
     4501 tggccagaca gagatggaac tgtaatacat agagcaagca gtctctactg ggatcctctg
     4561 ggcccccaga tgcctttatc agcactcagt ccaaactaag gatatcattc tttttttaat
     4621 tagatatttt ctttatttca aatgttattt cctttcctag tttcacctcc aaaaaccccc
     4681 tacccctcct ctctccccct gcttcccaac ccacccactc ccacttcctg gccttggcat
     4741 tcccctacac tgggcatcga gccttcacag gatcaagagc ctctcctccc attgatgact
     4801 gactaggcca ttctctgcta catatgcagc tagagccatg agtccctcca tgtgttttct
     4861 ttggttggtg gtttagtacc agggagctct ggggctactg gttagttcat attgtcgttc
     4921 ctcctagggg gctgcaaacc ccttcagctc ctggggtcct ttctctagct ccttcattgg
     4981 ggacaggata tcattcttat gcataaataa agtgccttga agggttcgga ggagagtggg
     5041 cgagcactgg tacgaagcct ttggacatgt gacaagtgcc atggttcttt agaggtctag
     5101 gagaagtagg gacagggagg tctcagggac agagaggtct tggttgttcc tttcttagtc
     5161 ccactgtaaa ggaaagagaa gaatatgggc tttggtgggg ctctgaggag tcccttgttc
     5221 ccagctctca gcttctactc cccaattctt ttaaacagac tgggtggatc tgagccaccc
     5281 tcaggggcta tctccctggc ccagaggctg gcagagtgct cagaagaacc tctgtctcca
     5341 tcccatttgc acccctgttg atctttcatc taccatagac tgaaagtttc agggtcttcc
     5401 tcacatggag cttaagagtc tcatgcaatg gccatgaaga actcagcgca gtgcccagta
     5461 ctccacaggc ctttggtacc agggcctggt tttattggta ggtagcatgg agtcacagcc
     5521 cagcctactt tctagtcatc tgcccggaat gggtacactt actggaaccg gctcacttgg
     5581 acatctctcc gctcagccca ttggctgggt tctgagtcag agctgacttt gcttttgacc
     5641 ttgctgtcat ttgttcccac ggactcccag gattcctggg tttcctgcca gggcccttgt
     5701 cccgtcagtg gccctttctg attccaggcg gcagtctctg cttctcttct ctgtcgttct
     5761 ccaggactgg ggcagcagct gagacctggg gccagtccat gactttttag ctatgagtga
     5821 ccttggctta ggacagtcac acagctccct cactctcttg ttcttagctg acccatgatg
     5881 ctttgtgtct gctgccaccc acaaccctaa actgtcaagt caggaagtcc tacaccaaac
     5941 cagtatccca ggtgtttcgt tcctgcttag ggtttgcttc ttcagtctcc ctggggtatt
     6001 tcatgttccc ctgtgacatg ctctctctcc attctcccct ctttagtctc tctgtcccac
     6061 tcttctccct aattctgggc ttcccactct cctcaggaga ccctctaatc atacgtatga
     6121 actttgccaa atgactcagc tgtctctgca gcgagcagtg ctctaagggt tataaaccag
     6181 tctgactaag gtgctaacaa gagcagcagc tccagggctc tcacgtgctt tgacaaagaa
     6241 ctccgaatga gtcgctagtg gtaggatttt aagctcctac agagttggga tatgggggtc
     6301 aaatatttga gaccaagctg tctttccccc tgctactact gtcagaggtc tgtttgcttc
     6361 gtgtattgag ggctccgctg ctaggttgaa atgtattcaa accatgacat cttcaggata
     6421 cagtagcatc ctatgtaaca gtaacaaacg tttgtcttca agtctatttt gtggtccctc
     6481 ttttatttgc tgtttacacg gtgtatattt ctctttcacc ttcaatctct gtatctctaa
     6541 cgtgagtctc ttgtacacac catatagttg gcacattttt tttaaaaaaa tctattttcc
     6601 aatatctttt aattggaatt tttgtttgca tttaatctga ttgataagaa aagattatat
     6661 ctgccacttt tctattttct tcatatttat atataatata cattttattt ctttgttttt
     6721 catcactacc ttctttccta ttaaatagat atttttctgg ctcatcattt cattatcttc
     6781 cctgctttat tttaaatata tatcctttcg gtacttgcag tgaggattac catgaacatt
     6841 tcaatttcta ataatatgtt tggatcaaga ataccttatt ttaataagac atgaaactct
     6901 gattatgtat acctctattc tcatcttctt tatgtatgta tgcattatat atacacacat
     6961 atatataatc aacagttata tcttcatata tcctgatcct ctcgtgtaaa tttataatta
     7021 ttgttttatg cattcatcct atactttgac ctttaaaggg agttagttat aagtggtcaa
     7081 catccagaga ataaatgtca ttggaatact catctatgaa tgggatgctt atatctcacc
     7141 ctacccaccc caaactcagg gactgtcaca gaagagagga tgataagatt gtaagagcca
     7201 gaggttgagg aggcctggac caaaacagca tcttctgaac atggcaggcc cactgcactc
     7261 atgaactcac agctgctgtg gttgcctgta gaagacctgc gcaagattaa gatggtcagc
     7321 gtcctggcat agagccagat gtgctcatga cccctgccct cagctgagga gctgctggtg
     7381 gcttctaggg agcgagaacc aggggtcacc cgtgctccag tggatggctc tatgttcatg
     7441 ggtacatgga cagcactaat tgaactcagt tactagaaac acaaaaggga ccagaagttg
     7501 aaagaatggg gagtgggttt ggggggattt gggagagaaa acgggtgaat atgatcagaa
     7561 atctatgtat gtatgaaata atcaaaaagt taataaaata tttattttaa aag
//
