LOCUS       NM_001080809            5585 bp    mRNA    linear   ROD 07-OCT-2024
DEFINITION  Mus musculus carbamoyl-phosphate synthetase 1 (Cps1), mRNA; nuclear
            gene for mitochondrial product.
ACCESSION   NM_001080809 XM_129769 XM_901578 XM_912076 XM_986847 XM_993446
            XM_993466
VERSION     NM_001080809.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 5585)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 5585)
  AUTHORS   Li,P., Kuo,N., Patel,R. and Omary,M.B.
  TITLE     Hypoosmosis alters hepatocyte mitochondrial morphology and induces
            selective release of carbamoyl phosphate synthetase 1
  JOURNAL   Am J Physiol Gastrointest Liver Physiol 325 (4), G334-G346 (2023)
   PUBMED   37489865
  REMARK    GeneRIF: Hypoosmosis alters hepatocyte mitochondrial morphology and
            induces selective release of carbamoyl phosphate synthetase 1.
REFERENCE   3  (bases 1 to 5585)
  AUTHORS   Soria,L.R., Makris,G., D'Alessio,A.M., De Angelis,A., Boffa,I.,
            Pravata,V.M., Rufenacht,V., Attanasio,S., Nusco,E., Arena,P.,
            Ferenbach,A.T., Paris,D., Cuomo,P., Motta,A., Nitzahn,M.,
            Lipshutz,G.S., Martinez-Pizarro,A., Richard,E., Desviat,L.R.,
            Haberle,J., van Aalten,D.M.F. and Brunetti-Pierri,N.
  TITLE     O-GlcNAcylation enhances CPS1 catalytic efficiency for ammonia and
            promotes ureagenesis
  JOURNAL   Nat Commun 13 (1), 5212 (2022)
   PUBMED   36064721
  REMARK    GeneRIF: O-GlcNAcylation enhances CPS1 catalytic efficiency for
            ammonia and promotes ureagenesis.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 5585)
  AUTHORS   Ogura,M., Nakamura,Y., Tanaka,D., Zhuang,X., Fujita,Y., Obara,A.,
            Hamasaki,A., Hosokawa,M. and Inagaki,N.
  TITLE     Overexpression of SIRT5 confirms its involvement in deacetylation
            and activation of carbamoyl phosphate synthetase 1
  JOURNAL   Biochem Biophys Res Commun 393 (1), 73-78 (2010)
   PUBMED   20097174
  REMARK    GeneRIF: Because ammonia generated during fasting is toxic, SIRT5
            protein might play a protective role by converting ammonia to
            non-toxic urea through deacetylation and activation of CPS1.
REFERENCE   5  (bases 1 to 5585)
  AUTHORS   Nakagawa,T., Lomb,D.J., Haigis,M.C. and Guarente,L.
  TITLE     SIRT5 Deacetylates carbamoyl phosphate synthetase 1 and regulates
            the urea cycle
  JOURNAL   Cell 137 (3), 560-570 (2009)
   PUBMED   19410549
  REMARK    GeneRIF: SIRT5 deacetylates CPS1 and upregulates its activity
REFERENCE   6  (bases 1 to 5585)
  AUTHORS   Helou,K., Das,A.T., Lamers,W.H., Hoovers,J.M., Szpirer,C.,
            Szpirer,J., Klinga-Levan,K. and Levan,G.
  TITLE     FISH mapping of three ammonia metabolism genes (Glul, Cps1, Glud1)
            in rat, and the chromosomal localization of GLUL in human and Cps1
            in mouse
  JOURNAL   Mamm Genome 8 (5), 362-364 (1997)
   PUBMED   9107685
REFERENCE   7  (bases 1 to 5585)
  AUTHORS   Hinsdale,M.E., Hamm,D.A. and Wood,P.A.
  TITLE     Effects of short-chain acyl-CoA dehydrogenase deficiency on
            development expression of metabolic enzyme genes in the mouse
  JOURNAL   Biochem Mol Med 57 (2), 106-115 (1996)
   PUBMED   8733888
REFERENCE   8  (bases 1 to 5585)
  AUTHORS   Warden,C.H., Mehrabian,M., He,K.Y., Yoon,M.Y., Diep,A., Xia,Y.R.,
            Wen,P.Z., Svenson,K.L., Sparkes,R.S. and Lusis,A.J.
  TITLE     Linkage mapping of 40 randomly isolated liver cDNA clones in the
            mouse
  JOURNAL   Genomics 18 (2), 295-307 (1993)
   PUBMED   8288233
REFERENCE   9  (bases 1 to 5585)
  AUTHORS   Tomomura,M., Imamura,Y., Horiuchi,M., Koizumi,T., Nikaido,H.,
            Hayakawa,J. and Saheki,T.
  TITLE     Abnormal expression of urea cycle enzyme genes in juvenile visceral
            steatosis (jvs) mice
  JOURNAL   Biochim Biophys Acta 1138 (2), 167-171 (1992)
   PUBMED   1540663
REFERENCE   10 (bases 1 to 5585)
  AUTHORS   Bhat,N.K. and Avadhani,N.G.
  TITLE     The transport and processing of carbamyl phosphate synthetase-I in
            mouse hepatic mitochondria
  JOURNAL   Biochem Biophys Res Commun 118 (2), 514-522 (1984)
   PUBMED   6200105
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BY790286.1, BC126969.1,
            AK028683.1 and AI046442.1.
            
            On Nov 2, 2013 this sequence version replaced NM_001080809.1.
            
            Summary: This gene encodes a protein localized to the inner
            mitochondrial matrix. The encoded protein plays a role in the
            detoxification of ammonia by catalyzing the first step in the urea
            cycle in which carbomyl-phosphate is synthesized from ammonia and
            bicarbonate. Carbamoyl-phosphate is subsequently converted to urea
            that is excreted by the kidneys. Deficiency of the encoded enzyme
            leads to an accumulation of ammonia in the blood. High levels of
            ammonia are toxic to the central nervous system and result in
            neurological disorders. [provided by RefSeq, Oct 2013].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC126969.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849377, SAMN00849378
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            RefSeq Select criteria             :: based on conservation,
                                                  expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-39                BY790286.1         2-40
            40-4743             BC126969.1         1-4704
            4744-5554           AK028683.1         2351-3161
            5555-5585           AI046442.1         22-52               c
FEATURES             Location/Qualifiers
     source          1..5585
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="1"
                     /map="1 33.75 cM"
     gene            1..5585
                     /gene="Cps1"
                     /gene_synonym="4732433M03Rik; CPS; D1Ucla3"
                     /note="carbamoyl-phosphate synthetase 1"
                     /db_xref="GeneID:227231"
                     /db_xref="MGI:MGI:891996"
     exon            1..264
                     /gene="Cps1"
                     /gene_synonym="4732433M03Rik; CPS; D1Ucla3"
                     /inference="alignment:Splign:2.1.0"
     CDS             139..4641
                     /gene="Cps1"
                     /gene_synonym="4732433M03Rik; CPS; D1Ucla3"
                     /EC_number="6.3.4.16"
                     /note="carbamoyl-phosphate synthase [ammonia],
                     mitochondrial; carbamoyl-phosphate synthetase I;
                     carbamoylphosphate synthetase I; CPSase I"
                     /codon_start=1
                     /product="carbamoyl-phosphate synthase [ammonia],
                     mitochondrial precursor"
                     /protein_id="NP_001074278.1"
                     /db_xref="CCDS:CCDS35605.1"
                     /db_xref="GeneID:227231"
                     /db_xref="MGI:MGI:891996"
                     /translation="MTRILTACKVVKTLKSGFGFANVTTKRQWDFSRPGIRLLSVKAK
                     TAHIVLEDGTKMKGYSFGHPSSVAGEVVFNTGLGGYPEALTDPAYKGQILTMANPIIG
                     NGGAPDTTARDELGLNKYMESDGIKVAGLLVLNYSNDYNHWLATKSLGQWLQEEKVPA
                     IYGVDTRMLTKIIRDKGTMLGKIEFEGQSVDFVDPNKQNLIAEVSTKDVKVFGKGNPT
                     KVVAVDCGIKNNVIRLLVKRGAEVHLVPWNHDFTQMEYDGLLIAGGPGNPALAQPLIQ
                     NVKKILESDRKEPLFGISTGNIITGLAAGAKSYKMSMANRGQNQPVLNITNRQAFITA
                     QNHGYALDNTLPAGWKPLFVNVNDQTNEGIMHESKPFFAVQFHPEVSPGPTDTEYLFD
                     SFFSLIKKGKGTTITSVLPKPALVASRVEVSKVLILGSGGLSIGQAGEFDYSGSQAVK
                     AMKEENVKTVLMNPNIASVQTNEVGLKQADAVYFLPITPQFVTEVIKAERPDGLILGM
                     GGQTALNCGVELFKRGVLKEYGVKVLGTSVESIMATEDRQLFSDKLNEINEKIAPSFA
                     VESMEDALKAADTIGYPVMIRSAYALGGLGSGICPNKETLIDLGTKAFAMTNQILVER
                     SVTGWKEIEYEVVRDADDNCVTVCNMENVDAMGVHTGDSVVVAPAQTLSNAEFQMLRR
                     TSVNVVRHLGIVGECNIQFALHPTSMEYCIIEVNARLSRSSALASKATGYPLAFIAAK
                     IALGIPLPEIKNVVSGKTSACFEPSLDYMVTKIPRWDLDRFHGTSSRIGSSMKSVGEV
                     MAIGRTFEESFQKALRMCHPSVDGFTPRLPMNKEWPANLDLKKELSEPSSTRIYAIAK
                     ALENNMSLDEIVRLTSIDKWFLYKMRDILNMDKTLKGLNSDSVTEETLRKAKEIGFSD
                     KQISKCLGLTEAQTRELRLKKNIHPWVKQIDTLAAEYPSVTNYLYVTYNGQEHDIKFD
                     EHGIMVLGCGPYHIGSSVEFDWCAVSSIRTLRQLGKKTVVVNCNPETVSTDFDECDKL
                     YFEELSLERILDIYHQEACNGCIISVGGQIPNNLAVPLYKNGVKIMGTSPLQIDRAED
                     RSIFSAVLDELKVAQAPWKAVNTLNEALEFANSVGYPCLLRPSYVLSGSAMNVVFSED
                     EMKRFLEEATRVSQEHPVVLTKFVEGAREVEMDAVGKEGRVISHAISEHVEDAGVHSG
                     DATLMLPTQTISQGAIEKVKDATRKIAKAFAISGPFNVQFLVKGNDVLVIECNLRASR
                     SFPFVSKTLGVDFIDVATKVMIGESIDEKRLPTLEQPIIPSDYVAIKAPMFSWPRLRD
                     ADPILRCEMASTGEVACFGEGIHTAFLKAMLSTGFKIPQKGILIGIQQSFRPRFLGVA
                     EQLHNEGFKLFATEATSDWLNANNVPATPVAWPSQEGQNPSLSSIRKLIRDGSIDLVI
                     NLPNNNTKFVHDNYVIRRTAVDSGIALLTNFQVTKLFAEAVQKSRTVDSKSLFHYRQY
                     SAGKAA"
     exon            265..374
                     /gene="Cps1"
                     /gene_synonym="4732433M03Rik; CPS; D1Ucla3"
                     /inference="alignment:Splign:2.1.0"
     exon            375..519
                     /gene="Cps1"
                     /gene_synonym="4732433M03Rik; CPS; D1Ucla3"
                     /inference="alignment:Splign:2.1.0"
     exon            520..609
                     /gene="Cps1"
                     /gene_synonym="4732433M03Rik; CPS; D1Ucla3"
                     /inference="alignment:Splign:2.1.0"
     exon            610..666
                     /gene="Cps1"
                     /gene_synonym="4732433M03Rik; CPS; D1Ucla3"
                     /inference="alignment:Splign:2.1.0"
     exon            667..759
                     /gene="Cps1"
                     /gene_synonym="4732433M03Rik; CPS; D1Ucla3"
                     /inference="alignment:Splign:2.1.0"
     exon            760..849
                     /gene="Cps1"
                     /gene_synonym="4732433M03Rik; CPS; D1Ucla3"
                     /inference="alignment:Splign:2.1.0"
     exon            850..978
                     /gene="Cps1"
                     /gene_synonym="4732433M03Rik; CPS; D1Ucla3"
                     /inference="alignment:Splign:2.1.0"
     exon            979..1085
                     /gene="Cps1"
                     /gene_synonym="4732433M03Rik; CPS; D1Ucla3"
                     /inference="alignment:Splign:2.1.0"
     exon            1086..1224
                     /gene="Cps1"
                     /gene_synonym="4732433M03Rik; CPS; D1Ucla3"
                     /inference="alignment:Splign:2.1.0"
     exon            1225..1302
                     /gene="Cps1"
                     /gene_synonym="4732433M03Rik; CPS; D1Ucla3"
                     /inference="alignment:Splign:2.1.0"
     exon            1303..1401
                     /gene="Cps1"
                     /gene_synonym="4732433M03Rik; CPS; D1Ucla3"
                     /inference="alignment:Splign:2.1.0"
     exon            1402..1497
                     /gene="Cps1"
                     /gene_synonym="4732433M03Rik; CPS; D1Ucla3"
                     /inference="alignment:Splign:2.1.0"
     exon            1498..1687
                     /gene="Cps1"
                     /gene_synonym="4732433M03Rik; CPS; D1Ucla3"
                     /inference="alignment:Splign:2.1.0"
     exon            1688..1845
                     /gene="Cps1"
                     /gene_synonym="4732433M03Rik; CPS; D1Ucla3"
                     /inference="alignment:Splign:2.1.0"
     exon            1846..1974
                     /gene="Cps1"
                     /gene_synonym="4732433M03Rik; CPS; D1Ucla3"
                     /inference="alignment:Splign:2.1.0"
     exon            1975..2119
                     /gene="Cps1"
                     /gene_synonym="4732433M03Rik; CPS; D1Ucla3"
                     /inference="alignment:Splign:2.1.0"
     exon            2120..2330
                     /gene="Cps1"
                     /gene_synonym="4732433M03Rik; CPS; D1Ucla3"
                     /inference="alignment:Splign:2.1.0"
     exon            2331..2529
                     /gene="Cps1"
                     /gene_synonym="4732433M03Rik; CPS; D1Ucla3"
                     /inference="alignment:Splign:2.1.0"
     exon            2530..2706
                     /gene="Cps1"
                     /gene_synonym="4732433M03Rik; CPS; D1Ucla3"
                     /inference="alignment:Splign:2.1.0"
     exon            2707..2825
                     /gene="Cps1"
                     /gene_synonym="4732433M03Rik; CPS; D1Ucla3"
                     /inference="alignment:Splign:2.1.0"
     exon            2826..2967
                     /gene="Cps1"
                     /gene_synonym="4732433M03Rik; CPS; D1Ucla3"
                     /inference="alignment:Splign:2.1.0"
     exon            2968..3033
                     /gene="Cps1"
                     /gene_synonym="4732433M03Rik; CPS; D1Ucla3"
                     /inference="alignment:Splign:2.1.0"
     exon            3034..3097
                     /gene="Cps1"
                     /gene_synonym="4732433M03Rik; CPS; D1Ucla3"
                     /inference="alignment:Splign:2.1.0"
     exon            3098..3279
                     /gene="Cps1"
                     /gene_synonym="4732433M03Rik; CPS; D1Ucla3"
                     /inference="alignment:Splign:2.1.0"
     exon            3280..3474
                     /gene="Cps1"
                     /gene_synonym="4732433M03Rik; CPS; D1Ucla3"
                     /inference="alignment:Splign:2.1.0"
     exon            3475..3542
                     /gene="Cps1"
                     /gene_synonym="4732433M03Rik; CPS; D1Ucla3"
                     /inference="alignment:Splign:2.1.0"
     exon            3543..3618
                     /gene="Cps1"
                     /gene_synonym="4732433M03Rik; CPS; D1Ucla3"
                     /inference="alignment:Splign:2.1.0"
     exon            3619..3696
                     /gene="Cps1"
                     /gene_synonym="4732433M03Rik; CPS; D1Ucla3"
                     /inference="alignment:Splign:2.1.0"
     exon            3697..3804
                     /gene="Cps1"
                     /gene_synonym="4732433M03Rik; CPS; D1Ucla3"
                     /inference="alignment:Splign:2.1.0"
     exon            3805..3894
                     /gene="Cps1"
                     /gene_synonym="4732433M03Rik; CPS; D1Ucla3"
                     /inference="alignment:Splign:2.1.0"
     exon            3895..4065
                     /gene="Cps1"
                     /gene_synonym="4732433M03Rik; CPS; D1Ucla3"
                     /inference="alignment:Splign:2.1.0"
     exon            4066..4140
                     /gene="Cps1"
                     /gene_synonym="4732433M03Rik; CPS; D1Ucla3"
                     /inference="alignment:Splign:2.1.0"
     exon            4141..4239
                     /gene="Cps1"
                     /gene_synonym="4732433M03Rik; CPS; D1Ucla3"
                     /inference="alignment:Splign:2.1.0"
     exon            4240..4299
                     /gene="Cps1"
                     /gene_synonym="4732433M03Rik; CPS; D1Ucla3"
                     /inference="alignment:Splign:2.1.0"
     exon            4300..4412
                     /gene="Cps1"
                     /gene_synonym="4732433M03Rik; CPS; D1Ucla3"
                     /inference="alignment:Splign:2.1.0"
     exon            4413..4542
                     /gene="Cps1"
                     /gene_synonym="4732433M03Rik; CPS; D1Ucla3"
                     /inference="alignment:Splign:2.1.0"
     exon            4543..5562
                     /gene="Cps1"
                     /gene_synonym="4732433M03Rik; CPS; D1Ucla3"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5536..5541
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Cps1"
                     /gene_synonym="4732433M03Rik; CPS; D1Ucla3"
                     /note="hexamer: AATAAA"
     polyA_site      5558
                     /gene="Cps1"
                     /gene_synonym="4732433M03Rik; CPS; D1Ucla3"
                     /note="major polyA site"
ORIGIN      
        1 agatcgctgt gcagtcagcc tacagcctca actgtactgt cgccacacag ctttctttca
       61 cactggttac aaacaaattg gacatcaaaa tctcatgaga tatttgtgat ttaattttag
      121 tcacaaaaca tcttcaaaat gacgaggatt ttgacagctt gcaaagtggt gaagaccctg
      181 aagagtggct ttggttttgc caatgtgact acgaagcgac agtgggactt ctccagacct
      241 ggcatcaggc tcctttctgt gaaggcaaag acagcacaca ttgttctgga agatggaact
      301 aagatgaaag gctactcctt tggccatccc tcctctgttg ctggtgaagt ggtttttaat
      361 actggcttag gagggtaccc ggaagcactt actgatcccg cctacaaggg gcagatcctc
      421 accatggcca accctatcat tgggaacggt ggggctcccg acaccactgc ccgagacgaa
      481 ctgggactga ataagtacat ggagtctgat gggatcaagg tggcaggtct gctggttctg
      541 aattacagta atgactataa ccactggctg gctaccaaga gtctgggtca atggctgcag
      601 gaggaaaagg tccctgcaat ttatggagtg gatacaagaa tgctgactaa aataattcgg
      661 gataagggta ccatgcttgg gaagattgaa tttgaaggcc agtctgtgga ttttgtggat
      721 cccaataagc agaatttgat tgctgaggtt tcaaccaagg atgtcaaggt gtttggcaaa
      781 ggaaacccca cgaaagtggt agctgtggac tgtgggataa aaaacaatgt catccgcctg
      841 ctagttaagc gaggagctga agtgcatttg gttccctgga atcacgactt cacccagatg
      901 gagtatgatg gacttttgat tgctggggga cctgggaatc cagctcttgc acagccacta
      961 attcagaatg tgaaaaagat tttggagagt gaccgcaaag agccattgtt tggaatcagt
     1021 acaggaaaca taataacagg attggctgct ggtgccaaat cctacaagat gtccatggcc
     1081 aacagaggac agaaccaacc tgttttgaat atcacaaaca gacaggcttt cataactgct
     1141 cagaatcatg gctatgctct ggacaacaca ctccctgctg gctggaaacc actgtttgtg
     1201 aatgtcaatg atcaaacaaa cgaggggatt atgcatgaga gcaaaccttt cttcgctgtg
     1261 cagttccacc cagaggtcag cccggggcca acagacactg agtacctatt tgattccttc
     1321 ttctccctga taaagaaggg caaaggcacc accattacct cagttctgcc caagccagcg
     1381 ttggtggcat ctcgtgtcga ggtttccaag gtcctcatcc tgggatcagg aggtctgtcc
     1441 attggtcagg caggtgaatt tgattactcg ggatctcaag ctgtgaaagc catgaaggaa
     1501 gaaaatgtca aaacagtcct gatgaacccg aacatcgcat ccgtgcagac caacgaggtg
     1561 ggattgaagc aggcagatgc agtctacttt ctccctatta ccccccagtt tgtcacagaa
     1621 gttatcaagg ctgaacggcc tgatgggctg attctgggca tgggtggcca gacagctctg
     1681 aactgtggag tggaactatt caagaggggt gtgctcaagg aatatggggt gaaggtcttg
     1741 ggcacatcag ttgagtccat tatggccaca gaagacaggc agctgttctc agacaagctg
     1801 aatgagatca acgagaagat cgcacccagt tttgcagtgg aatcaatgga ggatgccttg
     1861 aaggcagcag acaccattgg ctaccctgtg atgattcggt ctgcatatgc tctgggtggc
     1921 ttaggctctg gcatctgtcc caataaggag accttaatag accttggcac aaaagcattt
     1981 gctatgacca accagatcct ggtggagcgg tcagtgacag gttggaaaga aatagaatat
     2041 gaagttgtcc gagatgcaga tgataactgt gtcacagtct gtaacatgga aaatgttgac
     2101 gccatgggtg ttcacacagg tgactcggtt gtcgtggccc cagcccagac actctccaat
     2161 gcagagttcc agatgttgag acgcacttca gtcaatgttg ttcgtcactt gggcattgtg
     2221 ggcgaatgca acattcagtt tgctcttcac cccacttcca tggaatactg tatcattgaa
     2281 gtgaatgcca ggctgtcccg gagttctgcc ctggcctcca aggccactgg ctacccactg
     2341 gcattcattg cagcaaagat tgctctagga atcccacttc cagaaatcaa gaatgttgta
     2401 tctgggaaga cctcagcctg cttcgaacct agcctggatt acatggtgac caagattcct
     2461 cgctgggatc ttgaccgttt ccatggaaca tccagccgaa ttggtagctc aatgaaaagt
     2521 gtgggcgagg tcatggccat tggtcgcacc tttgaggaaa gtttccagaa ggctctgagg
     2581 atgtgccatc catctgtgga tgggttcact ccccggctcc caatgaataa ggaatggcca
     2641 gcaaacctgg atctgaagaa agagctgtct gagccctcca gcacccgcat ctacgccatt
     2701 gctaaggcct tggagaacaa catgtccctc gacgaaattg tgaggctcac gtccatcgac
     2761 aagtggtttc tgtacaagat gcgtgacatt ttaaacatgg ataagacact gaaagggctt
     2821 aacagtgact ctgttacaga agaaactctg agaaaggcca aggagattgg gttctcagac
     2881 aagcagattt caaaatgttt gggactgact gaagcccaga caagagagct gaggttaaag
     2941 aaaaacatcc acccttgggt taaacagatt gatacattgg ctgcagaata cccatcagta
     3001 acaaactacc tgtatgttac ctacaacggc caggagcacg atatcaaatt tgatgaacat
     3061 ggaataatgg ttctgggctg tggtccatac cacattggca gcagtgtgga atttgattgg
     3121 tgtgctgtct ccagtatccg tacactgcgc caacttggca agaagacagt ggtggtgaat
     3181 tgtaacccag agactgtgag cactgatttt gatgagtgtg acaaactcta ctttgaagag
     3241 ctgtctttgg agagaatcct agacatctac caccaggagg catgtaatgg ctgcatcata
     3301 tcagttggcg gccagattcc aaacaacttg gcggtcccgc tgtacaagaa tggtgtcaag
     3361 atcatgggta caagccctct gcagatcgac agggctgagg atcgctccat cttctcagct
     3421 gtcttagatg agctaaaggt ggcccaggct ccatggaaag ctgtgaacac tttgaatgaa
     3481 gcactggaat ttgcaaactc tgtgggctac ccctgcttac tgagaccttc ctatgttttg
     3541 agtgggtctg ccatgaacgt ggtattctct gaggatgaga tgaaaaggtt tcttgaggag
     3601 gccactcgag tctctcagga acacccggtg gtgctgacca agtttgtgga gggggcccgg
     3661 gaagtagaga tggacgctgt tggcaaagaa ggacgggtca tctcccatgc catctctgaa
     3721 catgttgaag atgcaggtgt ccactcagga gatgccaccc tgatgctacc tacacagacc
     3781 atcagccaag gagccattga aaaggtgaag gatgccacac gaaagattgc aaaagccttt
     3841 gccatctctg ggccattcaa tgtccagttt cttgtcaaag gaaatgatgt cttggtgatt
     3901 gagtgcaatc tgagagcatc aagatccttc ccctttgtct ccaagactct tggggtggac
     3961 ttcattgacg tggctacaaa ggtgatgatc ggggagagca ttgatgagaa gcgacttccc
     4021 acgctggagc agcccatcat tccctctgac tatgttgcca ttaaggctcc aatgttttcc
     4081 tggccccgac tgagagatgc tgaccctatt ctgagatgtg agatggcttc tactggagag
     4141 gtggcgtgct tcggcgaggg cattcatacc gccttcctaa aggccatgct gtctacaggg
     4201 tttaagatac ctcagaaggg cattctgatt ggaatccagc aatcattccg tccaagattc
     4261 cttggtgttg ctgagcagtt gcacaatgaa ggtttcaagc tttttgccac agaagctaca
     4321 tcagactggc tcaatgccaa caatgtccct gccacccctg tggcttggcc atctcaggaa
     4381 ggacagaatc ccagcctctc ttccatcaga aagttgataa gagatggaag cattgaccta
     4441 gtgattaacc tccccaataa caacaccaaa tttgtccatg ataattacgt gattcggagg
     4501 acagctgtgg acagtggaat tgctctcctc accaatttcc aggtgaccaa actttttgct
     4561 gaagctgtgc agaaatctcg cactgtggac tccaagagtc tgttccacta caggcagtac
     4621 agtgctggaa aggccgccta aagcagtatg ctggtctggt gaattcatcc tcgagtcagc
     4681 tcgagtcatg ctgcaccaaa gggactggtc ccagcctatc tccaggaatg gatggtgttg
     4741 ccaaacttta ttttggtttc ctttgttctt agtagtctgt atctttaacc actagattgt
     4801 tgtcagtcac tgtttcaaaa ctataggttc ttcccaagtc atttgttctt cacaaggaat
     4861 tcaaccattc acacattatc ttttgtagac tgacttaaca tttcatggtg ctggtttata
     4921 acgattgaag tagaaaaagc tgctgcccta tctcctgaat tctatactct aaacatactc
     4981 ttgttttaaa gtaacttcag tcacacccaa agtccacaaa caggacagat taccatgaaa
     5041 ttttgttaaa ataaaatatg gatttaattt atgaatggca tcatgatgct tgtgtattgt
     5101 tatttttttt tggcaatgat tctcacttat atagtcaacc tagaaatagc attttttctt
     5161 cccatgattc acaaagtgag attggatttc tttgatagat ccttgcttca ggttttagaa
     5221 gatgtcagca gatgacagag gacacagaag tggttatttt cttgatattc atgttggatt
     5281 ttaatataag tcaagtgaaa tgtataggct gattcacctt ctgctccaat ttggaaaccc
     5341 tgtagaagtt cctgttcctt ttgtaggatg gattctggca gtagcctgac tctcaccttg
     5401 cattctgtat accacacata ttacacatag ggcctccatt cctttacatg caacattttt
     5461 ttttctttct ttcaagatag catttgttgc ttttaagtgt tttggttatt acaatacttt
     5521 aattgtgaac ttttaaataa aatactatta agtgaaaatg caaaaaaaaa aaaaaaaaaa
     5581 aaaaa
//
