LOCUS       NM_025815               3324 bp    mRNA    linear   ROD 02-JUN-2024
DEFINITION  Mus musculus copine VIII (Cpne8), transcript variant 1, mRNA.
ACCESSION   NM_025815
VERSION     NM_025815.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3324)
  AUTHORS   Lloyd,S.E., Maytham,E.G., Grizenkova,J., Hummerich,H. and
            Collinge,J.
  TITLE     A Copine family member, Cpne8, is a candidate quantitative trait
            gene for prion disease incubation time in mouse
  JOURNAL   Neurogenetics 11 (2), 185-191 (2010)
   PUBMED   19795140
  REMARK    GeneRIF: Results identify Cpne8, a member of the copine family, as
            the most promising candidate gene for prion disease incubation
            time.
REFERENCE   2  (bases 1 to 3324)
  AUTHORS   Maitra,R., Grigoryev,D.N., Bera,T.K., Pastan,I.H. and Lee,B.
  TITLE     Cloning, molecular characterization, and expression analysis of
            Copine 8
  JOURNAL   Biochem Biophys Res Commun 303 (3), 842-847 (2003)
   PUBMED   12670487
  REMARK    GeneRIF: characterization of the human ortholog, CPNE8.
REFERENCE   3  (bases 1 to 3324)
  AUTHORS   Creutz,C.E., Tomsig,J.L., Snyder,S.L., Gautier,M.C., Skouri,F.,
            Beisson,J. and Cohen,J.
  TITLE     The copines, a novel class of C2 domain-containing,
            calcium-dependent, phospholipid-binding proteins conserved from
            Paramecium to humans
  JOURNAL   J Biol Chem 273 (3), 1393-1402 (1998)
   PUBMED   9430674
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BU846828.1, AK004559.1 and
            BB043122.1.
            
            On Sep 29, 2005 this sequence version replaced NM_025815.1.
            
            Summary: This gene encodes a member of the copine family of highly
            conserved, calcium-dependent phospholipid binding proteins. The
            encoded protein has two characteristic C2 domains and a VWFA domain
            and may play a role in membrane trafficking. A related pseudogene
            is found on chromosome 8. Alternatively spliced transcript variants
            encoding different isoforms have been described. [provided by
            RefSeq, Jul 2008].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK004559.1, BC138745.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-13                BU846828.1         4-16
            14-3322             AK004559.1         2-3310
            3323-3324           BB043122.1         312-313
FEATURES             Location/Qualifiers
     source          1..3324
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="15"
                     /map="15 45.21 cM"
     gene            1..3324
                     /gene="Cpne8"
                     /gene_synonym="1200003E11Rik; 1500031E20Rik"
                     /note="copine VIII"
                     /db_xref="GeneID:66871"
                     /db_xref="MGI:MGI:1914121"
     exon            1..221
                     /gene="Cpne8"
                     /gene_synonym="1200003E11Rik; 1500031E20Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             85..1818
                     /gene="Cpne8"
                     /gene_synonym="1200003E11Rik; 1500031E20Rik"
                     /note="isoform 1 is encoded by transcript variant 1;
                     copine-8"
                     /codon_start=1
                     /product="copine-8 isoform 1"
                     /protein_id="NP_080091.1"
                     /db_xref="CCDS:CCDS27758.1"
                     /db_xref="GeneID:66871"
                     /db_xref="MGI:MGI:1914121"
                     /translation="MWARDKCSVPQLEMDSRYTSATGIGDLNQLSAAIPATRVEVSVS
                     CRNLLDRDTFSKSDPICVLYTQAVGNKEWREFGRTEVIDNTLNPDFVRKFILDYFFEE
                     RENLRFDLYDVDSKSPNLSKHDFLGQVFCTLGEIVGSQGSRLEKPIVGIPGRKCGTII
                     LTAEELNCCRDAVLMQFCANKLDKKDFFGKSDPFLVFYRSNEDGSFTICHKTEVVKNT
                     LNPVWQAFKISVRALCNGDYDRTIKVEVYDWDRDGSHDFIGEFTTSYRELARGQSQFN
                     VYEVVNPKKKGKKKKYTNSGTVTLLSFLVETEVSFLDYIKGGTQINFTVAIDFTASNG
                     NPAQPTSLHYMNPYQLNAYGMALKAVGEIVQDYDSDKMFPALGFGAKLPPDGRISHEF
                     ALNGNPQNPYCDGIEGVMEAYYRSLKSVQLYGPTNFAPVINHVARYASSVKDGSQYFV
                     LLIVTDGVISDMAQTKESIVNASKLPMSIIIVGVGPAEFDAMVELDGDDVRVSSRGKY
                     AERDIVQFVPFRDYIDRSGNHILSMARLAKDVLAEIPEQFLSYMRARGIKPSPAPPPY
                     TPPTHVLQTQI"
     exon            222..262
                     /gene="Cpne8"
                     /gene_synonym="1200003E11Rik; 1500031E20Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            263..309
                     /gene="Cpne8"
                     /gene_synonym="1200003E11Rik; 1500031E20Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            310..413
                     /gene="Cpne8"
                     /gene_synonym="1200003E11Rik; 1500031E20Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            414..453
                     /gene="Cpne8"
                     /gene_synonym="1200003E11Rik; 1500031E20Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            454..530
                     /gene="Cpne8"
                     /gene_synonym="1200003E11Rik; 1500031E20Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            531..594
                     /gene="Cpne8"
                     /gene_synonym="1200003E11Rik; 1500031E20Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            595..698
                     /gene="Cpne8"
                     /gene_synonym="1200003E11Rik; 1500031E20Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            699..803
                     /gene="Cpne8"
                     /gene_synonym="1200003E11Rik; 1500031E20Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            804..845
                     /gene="Cpne8"
                     /gene_synonym="1200003E11Rik; 1500031E20Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            846..921
                     /gene="Cpne8"
                     /gene_synonym="1200003E11Rik; 1500031E20Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            922..975
                     /gene="Cpne8"
                     /gene_synonym="1200003E11Rik; 1500031E20Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            976..1037
                     /gene="Cpne8"
                     /gene_synonym="1200003E11Rik; 1500031E20Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1038..1084
                     /gene="Cpne8"
                     /gene_synonym="1200003E11Rik; 1500031E20Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1085..1266
                     /gene="Cpne8"
                     /gene_synonym="1200003E11Rik; 1500031E20Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1267..1394
                     /gene="Cpne8"
                     /gene_synonym="1200003E11Rik; 1500031E20Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1395..1497
                     /gene="Cpne8"
                     /gene_synonym="1200003E11Rik; 1500031E20Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1498..1555
                     /gene="Cpne8"
                     /gene_synonym="1200003E11Rik; 1500031E20Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1556..1629
                     /gene="Cpne8"
                     /gene_synonym="1200003E11Rik; 1500031E20Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1630..3324
                     /gene="Cpne8"
                     /gene_synonym="1200003E11Rik; 1500031E20Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2850..2855
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Cpne8"
                     /gene_synonym="1200003E11Rik; 1500031E20Rik"
                     /note="hexamer: AATAAA"
     polyA_site      2873
                     /gene="Cpne8"
                     /gene_synonym="1200003E11Rik; 1500031E20Rik"
                     /note="major polyA site"
     regulatory      3305..3310
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Cpne8"
                     /gene_synonym="1200003E11Rik; 1500031E20Rik"
                     /note="hexamer: ATTAAA"
     polyA_site      3323
                     /gene="Cpne8"
                     /gene_synonym="1200003E11Rik; 1500031E20Rik"
ORIGIN      
        1 gcctccttcc tctgcctcca ccctcacccc ttcccgtcgg tgcggagctc cgctccgagc
       61 agcgctcagc ccgccgcagc ttggatgtgg gctcgggaca agtgctccgt tcctcagctc
      121 gagatggaca gccgctacac cagcgccacg ggcatcgggg acttgaacca gctgagtgcg
      181 gccatccccg ccacacgagt ggaggtgtca gtgtcctgca gaaatctcct tgatagagat
      241 acattttcta agtcggatcc aatttgtgtc ctatatacac aagcagttgg gaataaagaa
      301 tggagagagt ttggaagaac tgaagtgatc gataatactt tgaatcctga ttttgtaaga
      361 aagtttatcc tggattattt ctttgaagaa agagagaatc ttcgttttga cttatatgat
      421 gttgattcta agagtcccaa cttgtccaaa catgactttc tgggacaagt gttttgtaca
      481 ttgggggaga ttgttggttc acagggaagt cgcctggaaa agccaatagt aggaattcca
      541 ggcaggaaat gtgggacaat catactgacg gcagaggagc taaactgctg cagggatgct
      601 gtcttgatgc agttctgtgc aaacaaattg gataagaagg atttctttgg gaagtcagat
      661 cctttcctgg tattttatcg aagcaatgag gatggaagct ttacaatttg ccacaagaca
      721 gaagtggtca aaaacactct aaatcctgtg tggcaagcgt tcaagatctc agtgcgagca
      781 ttgtgcaatg gggactatga cagaacaatc aaggtagaag tgtacgactg ggaccgagac
      841 gggagtcatg atttcattgg agaattcaca acaagctatc gggagctcgc aagagggcag
      901 tcacaattca acgtctatga ggtggtgaat cccaaaaaga aaggaaaaaa gaaaaaatac
      961 accaattcag gaacagtaac cttattgtcc ttcttggtgg aaacagaagt ctccttcctg
     1021 gactatatta aaggaggaac gcagatcaac ttcacggtgg ccattgattt tacagcatca
     1081 aatggaaacc ctgcccagcc aacttctctc cactatatga acccttacca actgaatgcc
     1141 tatggaatgg cactgaaggc tgtaggagaa atcgttcaag attatgacag tgataagatg
     1201 ttcccagccc tgggttttgg tgcaaagctg cctccagatg gaaggatatc tcacgagttt
     1261 gccctgaatg gcaaccctca gaatccttac tgtgatggca ttgaaggtgt catggaagct
     1321 tactatagaa gtctgaagtc tgtccagttg tatgggccaa ccaactttgc tcccgtaata
     1381 aaccacgtag caagatacgc atcctctgta aaagatggct cccagtactt tgtgcttctc
     1441 attgtgacgg acggcgtcat ctcagatatg gctcaaacta aagaatccat agttaatgcc
     1501 tcaaagcttc ccatgtcaat aataatagta ggtgtgggac ctgcagaatt tgatgcaatg
     1561 gttgaattgg atggagatga tgtaagagtt tcctccagag ggaaatatgc tgaaagagat
     1621 attgtacagt ttgtgccgtt cagggattat attgacagaa gtggaaacca catactcagc
     1681 atggcgagac tggccaaaga tgtcttagct gagatcccag agcagttcct ttcctacatg
     1741 agagccagag gaatcaagcc atcaccggcg cccccgccgt acacccctcc tacacacgtg
     1801 ctgcagacgc aaatatgacc acgcccagat gctgatgtcc ctcacacgcc agtcggaact
     1861 gcgaagcctg tgctttgatc ctggtgctct gctttgaacc agggaatgag acactttctc
     1921 agtttggttt cagcagtcct ggaaaatgtg ctttcttgga tccaaattac attctcttcc
     1981 taaaccaaaa ctgtaaatag ggttgttgca tgagcaaccg ggaaattgtt taagatgctt
     2041 gaagcaaagt agagatgtct tctctaattt ttgaatattt ttgttttttt gttcatgacg
     2101 gaatagtaaa tttccaatgt attattggga aaaagcacaa gctacatttt taacacaaat
     2161 gttgtccttg actgctacat atataggccg gcagtctctg tgtcgatgtt tacacgttac
     2221 tactttttaa atttcaatac ttttataact gttcttaaga gtaagtttta aatattgaat
     2281 cctttgtctt ctaaattgca atagctataa tcacaggagc aatatctctt tgaatgactg
     2341 tctggacatt catggtatga ataagggaag agaaggaggt agaatctact tagtgattaa
     2401 tatccatttt ggtcaaccgt gggagtttca acttataaac agatgtggcc ctgtaatatg
     2461 catttttttc atggactttg ggtttaaatt tagactgcag tcaatttatc tttcaaatgc
     2521 caactagaag caatgaacag gcaaacatcg tgaactgtta atgcacagaa tatactttgt
     2581 ataaaaacca ccatccttat aacactatga tttagtgtgt aaaaacaaga ctatataact
     2641 ccaaaccatg tagcaggtgt attgaacttt gtattcagtt atatcctgtc tagatatttt
     2701 gattcaaagg gatactggct ctcttgcttt ggaatttata ttctcaccct tccatcttgc
     2761 cttcacgggt tgcacaggct ggagtctctt gtgagttctc agttccacgt acacacaacc
     2821 ctgtgactta gcacacaaca cgtttgtgaa ataaactcat tcctgtgccc caacctgcct
     2881 gtttgtgaaa taacccatcc ctctaccctg acctgcctgc tcacaaccaa ttctaaatag
     2941 ttttcaatgt acagttttta tacgaattac agttttgtga agttacgtct acatttcttt
     3001 agaacaaatg gccttaaatt ctcacagaat tcctggaaat gattgtgaat tgccttcaaa
     3061 atacagaaat gtgtatttgt ttgttgttct tttttgtgtc aacagtgtga ctgctttcta
     3121 acctctctgc ctccccctct ttgctacttg gattcttcct attgtgtgtg ctcttgccac
     3181 aacttggatc tatggtgact tttataagca tgaagctgtt ttactggaga atatgcataa
     3241 tcctcatagc taacagagtc acagttacat aactgtgacg tgaattgtcc atttttaagt
     3301 acctattaaa acatttaaaa ggat
//
