LOCUS       NM_001360193            2185 bp    mRNA    linear   ROD 05-JUN-2024
DEFINITION  Mus musculus copine VI (Cpne6), transcript variant 4, mRNA.
ACCESSION   NM_001360193 XM_006518506
VERSION     NM_001360193.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2185)
  AUTHORS   Liu,P., Khvotchev,M., Li,Y.C., Chanaday,N.L. and Kavalali,E.T.
  TITLE     Copine-6 Binds to SNAREs and Selectively Suppresses Spontaneous
            Neurotransmission
  JOURNAL   J Neurosci 38 (26), 5888-5899 (2018)
   PUBMED   29802203
  REMARK    GeneRIF: we identify copine-6 as a specific regulator of
            spontaneous neurotransmission
REFERENCE   2  (bases 1 to 2185)
  AUTHORS   Larimore,J., Zlatic,S.A., Arnold,M., Singleton,K.S., Cross,R.,
            Rudolph,H., Bruegge,M.V., Sweetman,A., Garza,C., Whisnant,E. and
            Faundez,V.
  TITLE     Dysbindin Deficiency Modifies the Expression of GABA Neuron and Ion
            Permeation Transcripts in the Developing Hippocampus
  JOURNAL   Front Genet 8, 28 (2017)
   PUBMED   28344592
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2185)
  AUTHORS   Reinhard,J.R., Kriz,A., Galic,M., Angliker,N., Rajalu,M., Vogt,K.E.
            and Ruegg,M.A.
  TITLE     The calcium sensor Copine-6 regulates spine structural plasticity
            and learning and memory
  JOURNAL   Nat Commun 7, 11613 (2016)
   PUBMED   27194588
  REMARK    GeneRIF: links activity-triggered calcium signals to spine
            structural plasticity necessary for learning and memory
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2185)
  AUTHORS   Perestenko,P., Watanabe,M., Beusnard-Bee,T., Guna,P. and
            McIlhinney,J.
  TITLE     The second C2-domain of copine-2, copine-6 and copine-7 is
            responsible for their calcium-dependent membrane association
  JOURNAL   FEBS J 282 (19), 3722-3736 (2015)
   PUBMED   26175110
  REMARK    GeneRIF: The second C2-domain of copine-6 is responsible for
            calcium-dependent membrane association.
REFERENCE   5  (bases 1 to 2185)
  AUTHORS   Perestenko,P.V., Pooler,A.M., Noorbakhshnia,M., Gray,A., Bauccio,C.
            and Jeffrey McIlhinney,R.A.
  TITLE     Copines-1, -2, -3, -6 and -7 show different calcium-dependent
            intracellular membrane translocation and targeting
  JOURNAL   FEBS J 277 (24), 5174-5189 (2010)
   PUBMED   21087455
REFERENCE   6  (bases 1 to 2185)
  AUTHORS   Nakayama,T., Yaoi,T., Kuwajima,G., Yoshie,O. and Sakata,T.
  TITLE     Ca2(+)-dependent interaction of N-copine, a member of the two C2
            domain protein family, with OS-9, the product of a gene frequently
            amplified in osteosarcoma
  JOURNAL   FEBS Lett 453 (1-2), 77-80 (1999)
   PUBMED   10403379
REFERENCE   7  (bases 1 to 2185)
  AUTHORS   Nakayama,T., Yaoi,T. and Kuwajima,G.
  TITLE     Localization and subcellular distribution of N-copine in mouse
            brain
  JOURNAL   J Neurochem 72 (1), 373-379 (1999)
   PUBMED   9886090
REFERENCE   8  (bases 1 to 2185)
  AUTHORS   Nakayama,T., Yaoi,T., Yasui,M. and Kuwajima,G.
  TITLE     N-copine: a novel two C2-domain-containing protein with neuronal
            activity-regulated expression
  JOURNAL   FEBS Lett 428 (1-2), 80-84 (1998)
   PUBMED   9645480
REFERENCE   9  (bases 1 to 2185)
  AUTHORS   Creutz,C.E., Tomsig,J.L., Snyder,S.L., Gautier,M.C., Skouri,F.,
            Beisson,J. and Cohen,J.
  TITLE     The copines, a novel class of C2 domain-containing,
            calcium-dependent, phospholipid-binding proteins conserved from
            Paramecium to humans
  JOURNAL   J Biol Chem 273 (3), 1393-1402 (1998)
   PUBMED   9430674
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from CJ143652.1, AK144410.1,
            AK134548.1 and CJ286371.1.
            
            On Jan 30, 2018 this sequence version replaced XM_006518506.3.
            
            Summary: Calcium-dependent membrane-binding proteins may regulate
            molecular events at the interface of the cell membrane and
            cytoplasm. This gene is one of several genes that encodes a
            calcium-dependent protein containing two N-terminal type II C2
            domains and an integrin A domain-like sequence in the C-terminus.
            Alternative splicing results in multiple transcript variants
            encoding different isoforms. [provided by RefSeq, Jan 2018].
            
            Transcript Variant: This variant (4) differs in the 5' UTR compared
            to variant 1. Variants 1, 2 and 4 encode the same isoform (a).
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660825.36116.1,
                                           SRR7345562.1494868.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164131, SAMN01164132
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-303               CJ143652.1         1-303
            304-1778            AK144410.1         153-1627
            1779-2179           AK134548.1         1618-2018
            2180-2185           CJ286371.1         399-404
FEATURES             Location/Qualifiers
     source          1..2185
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="14"
                     /map="14 28.19 cM"
     gene            1..2185
                     /gene="Cpne6"
                     /note="copine VI"
                     /db_xref="GeneID:12891"
                     /db_xref="MGI:MGI:1334445"
     exon            1..265
                     /gene="Cpne6"
                     /inference="alignment:Splign:2.1.0"
     exon            266..437
                     /gene="Cpne6"
                     /inference="alignment:Splign:2.1.0"
     CDS             270..1943
                     /gene="Cpne6"
                     /note="isoform a is encoded by transcript variant 4;
                     copine-6; N-copine; neuronal-copine"
                     /codon_start=1
                     /product="copine-6 isoform a"
                     /protein_id="NP_001347122.1"
                     /db_xref="CCDS:CCDS27112.1"
                     /db_xref="GeneID:12891"
                     /db_xref="MGI:MGI:1334445"
                     /translation="MSDPEMGWVPEPPAMTLGASRVELRVSCHGLLDRDTLTKPHPCV
                     LLKLYSDEQWVEVERTEVLRSCSSPVFSRVLAIEYFFEEKQPLQFHVFDAEDGATSPS
                     SDTFLGSTECTLGQIVSQTKVTKPLLLKNGKTAGKSTITIVAEEVSGTNDYVQLTFRA
                     HKLDNKDLFSKSDPFMEIYKTNGDQSDQLVWRTEVVKNNLNPSWEPFRLSLHSLCSCD
                     IHRPLKFLVYDYDSSGKHDFIGEFTSTFQEMQEGTANPGQEMQWDCINPKYRDKKKNY
                     KSSGTVVLAQCTVEKVHTFLDYIMGGCQISFTVAIDFTASNGDPRSSQSLHCLSPRQP
                     NHYLQALRTVGGICQDYDSDKRFPAFGFGARIPPNFEVSHDFAINFDPENPECEEISG
                     VIASYRRCLPQIQLYGPTNVAPIINRVAEPAQREQSTGQATKYSVLLVLTDGVVSDMA
                     ETRTAIVRASRLPMSIIIVGVGNADFSDMRLLDGDDGPLRCPKGVPAARDIVQFVPFR
                     DFKDAAPSALAKCVLAEVPRQVVEYYASQGISPGAPRPSTPAMTPSPSP"
     exon            438..617
                     /gene="Cpne6"
                     /inference="alignment:Splign:2.1.0"
     exon            618..692
                     /gene="Cpne6"
                     /inference="alignment:Splign:2.1.0"
     exon            693..767
                     /gene="Cpne6"
                     /inference="alignment:Splign:2.1.0"
     exon            768..851
                     /gene="Cpne6"
                     /inference="alignment:Splign:2.1.0"
     exon            852..941
                     /gene="Cpne6"
                     /inference="alignment:Splign:2.1.0"
     exon            942..1046
                     /gene="Cpne6"
                     /inference="alignment:Splign:2.1.0"
     exon            1047..1133
                     /gene="Cpne6"
                     /inference="alignment:Splign:2.1.0"
     exon            1134..1193
                     /gene="Cpne6"
                     /inference="alignment:Splign:2.1.0"
     exon            1194..1327
                     /gene="Cpne6"
                     /inference="alignment:Splign:2.1.0"
     exon            1328..1382
                     /gene="Cpne6"
                     /inference="alignment:Splign:2.1.0"
     exon            1383..1434
                     /gene="Cpne6"
                     /inference="alignment:Splign:2.1.0"
     exon            1435..1568
                     /gene="Cpne6"
                     /inference="alignment:Splign:2.1.0"
     exon            1569..1805
                     /gene="Cpne6"
                     /inference="alignment:Splign:2.1.0"
     exon            1806..1981
                     /gene="Cpne6"
                     /inference="alignment:Splign:2.1.0"
     exon            1982..2185
                     /gene="Cpne6"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2158..2163
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Cpne6"
                     /note="hexamer: AATAAA"
     polyA_site      2181
                     /gene="Cpne6"
                     /note="major polyA site"
ORIGIN      
        1 tttgggcctg cccccagccc gccctcctcc ctagtactgt ggctccctcc caggcagtgt
       61 ccctgtccct cccctctcct tcctcccagc cagtctgtgc aggggggggg gagctggtgc
      121 tgcggccccc ctcctcccca tccaggcccc ataaatagca gcagaaccga gccggagctg
      181 gcgccagcgg ctggagcagc gaggaagtca ggggcagggc ggaagactgc agactggaga
      241 gaggagaccc ccactaagag cccagtgcca tgtcggaccc agagatggga tgggtgcctg
      301 agcccccggc catgaccctg ggagcctctc gagtggagct gcgggtgtcc tgccatggcc
      361 tgctggaccg agacacgctc acaaagccgc atccatgtgt gctgctcaaa ctctactcgg
      421 atgagcagtg ggtggaggtg gaacgcacgg aggtgcttcg ctcctgttca agcccagtct
      481 tctcccgggt gctggccatt gaatactttt ttgaagagaa gcagcctttg cagttccacg
      541 tgttcgatgc cgaggatgga gccaccagcc ccagcagcga caccttcctc gggtctacgg
      601 agtgcacctt gggccagatt gtgtcacaaa ccaaggtcac taagccatta ctgctgaaga
      661 atgggaagac ggcgggcaag tctactatca cgattgtggc tgaggaggta tcaggtacca
      721 acgactatgt gcaacttacc ttcagagccc acaagctgga taacaaggat ctgttcagca
      781 agtctgaccc cttcatggag atttataaga ccaatggaga ccagagtgac caactggtct
      841 ggaggactga ggtggtgaaa aacaacctga accccagctg ggagccattc cgcctgtcct
      901 tgcattcttt gtgcagctgt gacatccaca ggccgctcaa gttcctggta tatgactatg
      961 actccagtgg aaagcacgac ttcatcggcg agttcaccag cacattccag gaaatgcaag
     1021 aggggaccgc aaaccctggg caggagatgc agtgggactg tatcaacccc aagtaccgag
     1081 acaagaagaa gaattacaag agctcaggga cagtcgtgct ggcccagtgc accgtggaaa
     1141 aagtccacac cttcctggat tatatcatgg gtggctgcca gatcagcttc acggtggcta
     1201 tcgacttcac tgcctccaat ggggacccaa ggagcagcca gtctctgcac tgcctcagcc
     1261 cccgacagcc caaccactac ctgcaggcct tgcgcacagt gggcggtatc tgccaggact
     1321 atgacagtga taagcggttc ccagcttttg gcttcggagc tcgaatcccc ccaaactttg
     1381 aggtctccca tgactttgct atcaactttg acccagaaaa tcctgaatgt gaagagatct
     1441 caggggtcat agcctcctac cgccgctgct tgcctcagat ccagctctat ggtcctacca
     1501 acgtggcccc tatcatcaac cgtgtggctg aaccagccca gcgagagcag agcaccggcc
     1561 aagccacgaa gtattcagta ctgctggtgc tcactgacgg tgtggtgagt gatatggcag
     1621 agacccgaac agccattgtg cgagcctccc gcctgcccat gtcaatcatc atcgtgggtg
     1681 tgggcaacgc tgacttctct gacatgaggc tactggacgg agatgatggt cccctgcgtt
     1741 gcccaaaggg ggtacctgca gcccgtgaca ttgtccagtt tgtgcccttc agggacttca
     1801 aggacgctgc cccctctgca ctagctaagt gtgtcctggc agaggtgcca cggcaggtgg
     1861 tagagtacta tgccagccaa ggtatcagtc ccggggctcc caggccctcc acgccagcta
     1921 tgactcccag ccctagccca tgactgcctc tcgacataac caacacccct tcagtctctc
     1981 agtgcctgtg ctgaccttgt gattccagtg acctgtgtct ctgcattttg gaccagtggt
     2041 gccctgcggg tcccggaagt gagtgatggg ccctggtccc atctctccaa gcccgatatc
     2101 cctcaactct gaggaccctc aataacttcc tccagtggtt gtgactttat ggatgtaaat
     2161 aaaaggaacc actaccgagt acccc
//
