LOCUS       NM_028719               3787 bp    mRNA    linear   ROD 12-FEB-2025
DEFINITION  Mus musculus copine IV (Cpne4), transcript variant 2, mRNA.
ACCESSION   NM_028719 XM_135115
VERSION     NM_028719.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3787)
  AUTHORS   Shigesawa,I., Nakamura,A., Yamauchi,Y., Kawata,S., Miyazaki,A.,
            Nomoto,H., Kameda,H., Terauchi,Y. and Atsumi,T.
  TITLE     Effects of glucokinase haploinsufficiency on the pancreatic
            beta-cell mass and function of long-term high-fat, high-sucrose
            diet-fed mice
  JOURNAL   J Diabetes Investig 15 (12), 1732-1742 (2024)
   PUBMED   39305123
REFERENCE   2  (bases 1 to 3787)
  AUTHORS   Bedogni,F. and Hevner,R.F.
  TITLE     Cell-Type-Specific Gene Expression in Developing Mouse Neocortex:
            Intermediate Progenitors Implicated in Axon Development
  JOURNAL   Front Mol Neurosci 14, 686034 (2021)
   PUBMED   34321999
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3787)
  AUTHORS   Ogujiofor,O.W., Pop,I.V., Espinosa,F., Durodoye,R.O.,
            Viacheslavov,M.L., Jarvis,R., Landy,M.A., Gurumurthy,C.B. and
            Lai,H.C.
  TITLE     An Atoh1 CRE Knock-In Mouse Labels Motor Neurons Involved in Fine
            Motor Control
  JOURNAL   eNeuro 8 (1) (2021)
   PUBMED   33468540
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3787)
  AUTHORS   Koopmans,F., van Nierop,P., Andres-Alonso,M., Byrnes,A.,
            Cijsouw,T., Coba,M.P., Cornelisse,L.N., Farrell,R.J.,
            Goldschmidt,H.L., Howrigan,D.P., Hussain,N.K., Imig,C., de
            Jong,A.P.H., Jung,H., Kohansalnodehi,M., Kramarz,B., Lipstein,N.,
            Lovering,R.C., MacGillavry,H., Mariano,V., Mi,H., Ninov,M.,
            Osumi-Sutherland,D., Pielot,R., Smalla,K.H., Tang,H., Tashman,K.,
            Toonen,R.F.G., Verpelli,C., Reig-Viader,R., Watanabe,K., van
            Weering,J., Achsel,T., Ashrafi,G., Asi,N., Brown,T.C., De
            Camilli,P., Feuermann,M., Foulger,R.E., Gaudet,P., Joglekar,A.,
            Kanellopoulos,A., Malenka,R., Nicoll,R.A., Pulido,C., de
            Juan-Sanz,J., Sheng,M., Sudhof,T.C., Tilgner,H.U., Bagni,C.,
            Bayes,A., Biederer,T., Brose,N., Chua,J.J.E., Dieterich,D.C.,
            Gundelfinger,E.D., Hoogenraad,C., Huganir,R.L., Jahn,R.,
            Kaeser,P.S., Kim,E., Kreutz,M.R., McPherson,P.S., Neale,B.M.,
            O'Connor,V., Posthuma,D., Ryan,T.A., Sala,C., Feng,G., Hyman,S.E.,
            Thomas,P.D., Smit,A.B. and Verhage,M.
  TITLE     SynGO: An Evidence-Based, Expert-Curated Knowledge Base for the
            Synapse
  JOURNAL   Neuron 103 (2), 217-234 (2019)
   PUBMED   31171447
REFERENCE   5  (bases 1 to 3787)
  AUTHORS   Mendelsohn,A.I., Dasen,J.S. and Jessell,T.M.
  TITLE     Divergent Hox Coding and Evasion of Retinoid Signaling Specifies
            Motor Neurons Innervating Digit Muscles
  JOURNAL   Neuron 93 (4), 792-805 (2017)
   PUBMED   28190640
REFERENCE   6  (bases 1 to 3787)
  AUTHORS   Pietri,S., Dimidschstein,J., Tiberi,L., Sotiropoulou,P.A.,
            Bilheu,A., Goffinet,A., Achouri,Y., Tissir,F., Blanpain,C.,
            Jacquemin,P. and Vanderhaeghen,P.
  TITLE     Transcriptional mechanisms of EphA7 gene expression in the
            developing cerebral cortex
  JOURNAL   Cereb Cortex 22 (7), 1678-1689 (2012)
   PUBMED   21940705
REFERENCE   7  (bases 1 to 3787)
  AUTHORS   van der Zwaag,B., Burbach,J.P., Scharfe,C., Oefner,P.J.,
            Brunner,H.G., Padberg,G.W. and van Bokhoven,H.
  TITLE     Identifying new candidate genes for hereditary facial paresis on
            chromosome 3q21-q22 by RNA in situ hybridization in mouse
  JOURNAL   Genomics 86 (1), 55-67 (2005)
   PUBMED   15953540
REFERENCE   8  (bases 1 to 3787)
  AUTHORS   Tomsig,J.L., Snyder,S.L. and Creutz,C.E.
  TITLE     Identification of targets for calcium signaling through the copine
            family of proteins. Characterization of a coiled-coil
            copine-binding motif
  JOURNAL   J Biol Chem 278 (12), 10048-10054 (2003)
   PUBMED   12522145
REFERENCE   9  (bases 1 to 3787)
  AUTHORS   Creutz,C.E., Tomsig,J.L., Snyder,S.L., Gautier,M.C., Skouri,F.,
            Beisson,J. and Cohen,J.
  TITLE     The copines, a novel class of C2 domain-containing,
            calcium-dependent, phospholipid-binding proteins conserved from
            Paramecium to humans
  JOURNAL   J Biol Chem 273 (3), 1393-1402 (1998)
   PUBMED   9430674
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC160113.2, AC160135.2 and AC117688.10.
            
            On Mar 17, 2020 this sequence version replaced NM_028719.2.
            
            Transcript Variant: This variant (2) differs in the 5' UTR and
            coding sequence compared to variant 1. The resulting isoform (2) is
            shorter at the N-terminus compared to isoform 1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC063081.1, AK043692.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849385, SAMN01164131
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-447               AC160113.2         181533-181979       c
            448-628             AC160113.2         65029-65209         c
            629-808             AC160135.2         173161-173340
            809-880             AC160135.2         200700-200771
            881-955             AC160135.2         201926-202000
            956-1039            AC117688.10        163886-163969       c
            1040-1129           AC117688.10        160623-160712       c
            1130-1228           AC117688.10        96792-96890         c
            1229-1315           AC117688.10        93248-93334         c
            1316-1375           AC117688.10        92575-92634         c
            1376-1509           AC117688.10        78795-78928         c
            1510-1564           AC117688.10        67933-67987         c
            1565-1616           AC117688.10        66645-66696         c
            1617-1750           AC117688.10        64041-64174         c
            1751-1987           AC117688.10        59523-59759         c
            1988-3787           AC117688.10        51918-53717         c
FEATURES             Location/Qualifiers
     source          1..3787
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="9"
                     /map="9 56.61 cM"
     gene            1..3787
                     /gene="Cpne4"
                     /gene_synonym="3632411M23Rik; 4933406O10Rik"
                     /note="copine IV"
                     /db_xref="GeneID:74020"
                     /db_xref="MGI:MGI:1921270"
     exon            1..447
                     /gene="Cpne4"
                     /gene_synonym="3632411M23Rik; 4933406O10Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            448..628
                     /gene="Cpne4"
                     /gene_synonym="3632411M23Rik; 4933406O10Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             449..2122
                     /gene="Cpne4"
                     /gene_synonym="3632411M23Rik; 4933406O10Rik"
                     /note="isoform 2 is encoded by transcript variant 2;
                     copine-4"
                     /codon_start=1
                     /product="copine-4 isoform 2"
                     /protein_id="NP_082995.1"
                     /db_xref="CCDS:CCDS23461.1"
                     /db_xref="GeneID:74020"
                     /db_xref="MGI:MGI:1921270"
                     /translation="MKKMSNIYESAANTLGIFNSPCLTKVELRVACKGISDRDALSKP
                     DPCVILKMQSHGQWFEVDRTEVIRTCINPVYSKLFTVDFYFEEVQRLRFEVHDISSNH
                     NGLKEADFLGGMECTLGQIVSQRKLSKSLLKHGNTAGKSSITVIAEELSGNDDYVELA
                     FNARKLDDKDFFSKSDPFLEIFRMNDDATQQLVHRTEVVMNNLSPAWKSFKVSVNSLC
                     SGDPDRRLKCIVWDWDSNGKHDFIGEFTSTFKEMRGAMEGKQVQWECINPKYKAKKKN
                     YKNSGMVILNQCKIHKMHSFLDYIMGGCQIQFTVAIDFTASNGDPRNSCSLHYIHPYQ
                     PNEYLKALVAVGEICQDYDSDKMFPAFGFGARIPPEYTVSHDFAINFNEDNPECAGIQ
                     GVVEAYQSCLPKLQLYGPTNIAPIIQKVAKSASEETNTKEASQYFILLILTDGVITDM
                     ADTREAIVHASHLPMSVIIVGVGNADFSDMQMLDGDDGILRSPKGEPVLRDIVQFVPF
                     RNFKHASPAALAKSVLAEVPNQVVDYYNGKGIKPKCSSEVYESSRTLAP"
     exon            629..808
                     /gene="Cpne4"
                     /gene_synonym="3632411M23Rik; 4933406O10Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            809..880
                     /gene="Cpne4"
                     /gene_synonym="3632411M23Rik; 4933406O10Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            881..955
                     /gene="Cpne4"
                     /gene_synonym="3632411M23Rik; 4933406O10Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            956..1039
                     /gene="Cpne4"
                     /gene_synonym="3632411M23Rik; 4933406O10Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1040..1129
                     /gene="Cpne4"
                     /gene_synonym="3632411M23Rik; 4933406O10Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1130..1228
                     /gene="Cpne4"
                     /gene_synonym="3632411M23Rik; 4933406O10Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1229..1315
                     /gene="Cpne4"
                     /gene_synonym="3632411M23Rik; 4933406O10Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1316..1375
                     /gene="Cpne4"
                     /gene_synonym="3632411M23Rik; 4933406O10Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1376..1509
                     /gene="Cpne4"
                     /gene_synonym="3632411M23Rik; 4933406O10Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1510..1564
                     /gene="Cpne4"
                     /gene_synonym="3632411M23Rik; 4933406O10Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1565..1616
                     /gene="Cpne4"
                     /gene_synonym="3632411M23Rik; 4933406O10Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1617..1750
                     /gene="Cpne4"
                     /gene_synonym="3632411M23Rik; 4933406O10Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1751..1987
                     /gene="Cpne4"
                     /gene_synonym="3632411M23Rik; 4933406O10Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1988..3787
                     /gene="Cpne4"
                     /gene_synonym="3632411M23Rik; 4933406O10Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3766..3771
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Cpne4"
                     /gene_synonym="3632411M23Rik; 4933406O10Rik"
                     /note="hexamer: AATAAA"
     polyA_site      3787
                     /gene="Cpne4"
                     /gene_synonym="3632411M23Rik; 4933406O10Rik"
                     /note="major polyA site"
ORIGIN      
        1 gtcagaaggt ggaaatctca gctagaagct gccatctcct ggctctcacc cctccctccc
       61 tcccttcctc ctttccttcc tccctcactc ctctgctccc tccctccttc cccagttcct
      121 tcctctttac tcagcgagtt cctgagccca gcagctcctt ccacgccgtt caccctctcc
      181 cccagccccc agcccccagc cccaagaatg gagtcggggt ttccagagcg accaaactct
      241 cctcccagca tctttatcct cctcggctgt tgctaaacta cccccagtgg accaaagagg
      301 ggggagggaa gcccgactct cctaagacct ctttacaact acatcaccca ggacctccac
      361 tccaaaggcg gattttgcat cctgggggcg tgacagacct gtcccgggct gaattctctt
      421 tctagtctct gaggctggca cacccagaat gaagaagatg agcaacattt atgagtcggc
      481 tgccaacacg ctgggaatct ttaacagccc ctgcctgacc aaagtcgagc tgcgtgtggc
      541 atgcaaaggc atttccgaca gagatgccct gtccaagccg gacccttgtg tcatcctcaa
      601 gatgcaatcc cacgggcaat ggtttgaggt ggacaggacg gaggtgattc gtacctgcat
      661 aaacccagtg tactcaaagc tgtttacagt ggacttttat tttgaagagg tccaacgtct
      721 ccggtttgaa gttcatgaca tcagcagcaa ccacaatgga ctgaaggaag ctgacttcct
      781 tggtggcatg gagtgcacac ttggccagat tgtctcacag agaaagctct ccaagtcctt
      841 gctgaagcat gggaacacag cggggaaatc ctccatcacg gtgattgctg aagaattatc
      901 gggcaatgac gactatgttg agcttgcatt caatgcacgg aaattggatg ataaggattt
      961 cttcagtaaa tcagacccat ttctggaaat tttcagaatg aacgatgacg cgactcaaca
     1021 gcttgtgcat cgaacagagg ttgtgatgaa taacttaagc ccagcctgga agtcattcaa
     1081 agtatcggta aactctctat gcagtggaga tccagaccgc aggctgaagt gcatagtgtg
     1141 ggactgggac tccaacggca agcatgactt catcggagag ttcacctcca ccttcaagga
     1201 gatgagagga gccatggaag ggaaacaggt acagtgggaa tgcatcaacc ccaagtacaa
     1261 agccaagaag aaaaattaca agaactcagg catggtgatt ctaaaccagt gcaagatcca
     1321 caagatgcat tccttcttgg actacatcat gggcggctgt caaatccagt ttacagtagc
     1381 tatagacttc actgcctcta atggggaccc cagaaacagc tgttccctgc actatatcca
     1441 cccttaccag cccaatgagt acctaaaggc cttggtggct gtgggagaga tttgccaaga
     1501 ctatgacagt gacaaaatgt tcccggcttt tgggtttggc gccaggatac ccccggaata
     1561 cacggtctct catgactttg caatcaactt taatgaagac aacccggagt gtgcaggaat
     1621 tcaaggagtc gtggaagcct atcagagctg tcttcccaag ctccagcttt atggtcccac
     1681 caacatcgct cccatcattc agaaggttgc caagtcagcc tcggaggaga caaacaccaa
     1741 ggaagcatcg caatacttca tcctgctgat cctgacagac ggggtcatca cagacatggc
     1801 tgacacccgg gaggccatcg tccacgcctc ccacctcccc atgtcagtca tcatcgttgg
     1861 ggtggggaac gccgacttca gtgacatgca gatgctggac ggtgacgacg gaattctaag
     1921 gtcacccaag ggagaacctg tccttcgaga catcgtccag tttgtgccct tccggaactt
     1981 caaacatgca tcgccagctg cccttgccaa gagtgtgttg gctgaagtcc caaatcaagt
     2041 cgtagactat tacaatggta aagggattaa accaaagtgc tcatcggaag tatatgaatc
     2101 gtcccggaca ctggcaccgt gaactacact tttacagaat tctgaagcac tgtttatcta
     2161 ttcctgtact ttaaataaca acaaaaacaa aacaaaaaaa aacaataata tacatttaaa
     2221 gatagcacgt tttggtgatt tttaattaac tgacttttta ttttatttta ttttttgcat
     2281 gtgtagccca aggcctggat ctgttaaacc cttgaattgt taaagacttt ttacaatgaa
     2341 atacagttat aacacactct ttacagcatg tcgccttgaa attaaaatgg tatatgtatc
     2401 catttttata ccggtttgtt gaaattttgc taaatttctt attatcttta cactgtaaag
     2461 cattttgaaa catttcgtga atgtcgatag ccaaaacgta tatttggctt aatctcctat
     2521 aggatgagag acttttcaaa atggcagatg catggactgt attttgcatg tttaaaataa
     2581 ttataataaa aataacctta cactgttttt gcagttgtta tctataatct ctccctgctg
     2641 agaatggtct ctctctacta aataaattta tgtacctaga ggtagtcttt tttgaagcgg
     2701 gcctgggtta tttatcccag tacccatctt tacacactcc cattgacaca aattcctgga
     2761 caatcttgtt ctaatgaagc tttctaatgc ggggcctgag gttctgcaca tctcacaagg
     2821 tcctcaatga tgctagtgca tcccttgcat ggcatgagga ctaatggggc tctgaacttg
     2881 aggctttcac ctcagagatc atgccaaaca gaacagctcc tagcctgctt gcccatggca
     2941 cctcttccac ccaccaggct ccccagtagc ttagaggacc agcctgtctc caagatgaga
     3001 ctagaaagta aggagactga ttccagaagc tgtccacccg acatcccggc ctcgctgttt
     3061 tatagcccca cctcttcctc atccggactg aggagcacgc cttctggttt gctcctgctt
     3121 ggctaaaagg ggcagcacag ctctctggat gggtggttcc attttttatg ccagttccta
     3181 gaaattattt cccaagattc ctcccttgga tctttgagtg ggatcctctt catgccaaat
     3241 agcaacatgg ctgtccaact ttccgttcta atcgcttggt ctttgagctc actgtggggg
     3301 ccttttttga cctcttttgt atcactcctg agtacaattt ccataagcag aagtgccctg
     3361 gaattgtgta gggtggagat attttgtctg aatctaagga agtctaaaac caaaataatg
     3421 acttcctctt ctcttatgat attctctccg agaaatggac aaatgtttta taagctataa
     3481 aaggcacaaa atgtaactta tttttggtca gaaaaataga gaagtgtatc caaagcaaaa
     3541 gaaatctgtt gttggggtag tttgggttct ttttcttttt ttttgaagcg gaaacaataa
     3601 taattaatta agcaaatcaa atgtcttgta caatttccag agttataatt atagattatc
     3661 ttgcaaatac atgtcaacca aatgcataac attttgggaa tgatggttta aaaaatgtac
     3721 gacagaaaaa aacagtcaca aatgtttaaa ttgtattgtt ttctgaataa attgggtact
     3781 tacagaa
//
