LOCUS       NM_027904               4583 bp    mRNA    linear   ROD 02-JUN-2024
DEFINITION  Mus musculus carboxypeptidase N, polypeptide 2 (Cpn2), mRNA.
ACCESSION   NM_027904 XM_148373 XM_358824
VERSION     NM_027904.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4583)
  AUTHORS   Naba,A., Clauser,K.R., Hoersch,S., Liu,H., Carr,S.A. and Hynes,R.O.
  TITLE     The matrisome: in silico definition and in vivo characterization by
            proteomics of normal and tumor extracellular matrices
  JOURNAL   Mol Cell Proteomics 11 (4), M111.014647 (2012)
   PUBMED   22159717
REFERENCE   2  (bases 1 to 4583)
  AUTHORS   Bernhard,O.K., Kapp,E.A. and Simpson,R.J.
  TITLE     Enhanced analysis of the mouse plasma proteome using
            cysteine-containing tryptic glycopeptides
  JOURNAL   J Proteome Res 6 (3), 987-995 (2007)
   PUBMED   17330941
REFERENCE   3  (bases 1 to 4583)
  AUTHORS   Ghesquiere,B., Van Damme,J., Martens,L., Vandekerckhove,J. and
            Gevaert,K.
  TITLE     Proteome-wide characterization of N-glycosylation events by
            diagonal chromatography
  JOURNAL   J Proteome Res 5 (9), 2438-2447 (2006)
   PUBMED   16944957
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK005049.1 and AC163355.2.
            
            On May 22, 2007 this sequence version replaced NM_027904.2.
            
            Sequence Note: An in-frame AUG is located 2 codons upstream of the
            annotated translation start site but is not being annotated as a
            start site since it is not conserved and is in a weak Kozak
            sequence context.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC025836.1, AK005049.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849375, SAMN00849377
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            CDS uses downstream in-frame AUG :: upstream AUG and CDS extension
                                                is not conserved
            RefSeq Select criteria           :: based on single protein-coding
                                                transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2072              AK005049.1         3-2074
            2073-4583           AC163355.2         188416-190926       c
FEATURES             Location/Qualifiers
     source          1..4583
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="16"
                     /map="16 21.22 cM"
     gene            1..4583
                     /gene="Cpn2"
                     /gene_synonym="1300018K11Rik"
                     /note="carboxypeptidase N, polypeptide 2"
                     /db_xref="GeneID:71756"
                     /db_xref="MGI:MGI:1919006"
     exon            1..76
                     /gene="Cpn2"
                     /gene_synonym="1300018K11Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            77..4583
                     /gene="Cpn2"
                     /gene_synonym="1300018K11Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             80..1723
                     /gene="Cpn2"
                     /gene_synonym="1300018K11Rik"
                     /EC_number="3.4.17.3"
                     /note="carboxypeptidase N, polypeptide 2 homolog;
                     carboxypeptidase N subunit 2; carboxypeptidase N 83 kDa
                     chain; carboxypeptidase N large subunit; carboxypeptidase
                     N regulatory subunit"
                     /codon_start=1
                     /product="carboxypeptidase N subunit 2 precursor"
                     /protein_id="NP_082180.2"
                     /db_xref="CCDS:CCDS49819.1"
                     /db_xref="GeneID:71756"
                     /db_xref="MGI:MGI:1919006"
                     /translation="MFPGAWLCWVSLLLLARLTQPCPVGCDCFGREVFCSDEQLADIP
                     PDIPPHITDIVFVETAFTTVRTRAFSGSPNLTKVVFLNTQVRHLEPDAFGGLPRLQDL
                     EITGSPVSNLSAHIFSNLSSLEKLTLDFDRLAGLPEDLFCHMDILESLQLQGNQLRTL
                     PGRLFQSLRDLRTLNLAQNLLTQLPKGAFQSLTGLQMLKLSNNMLARLPEGALGSLSS
                     LQELFLDGNAITELSPHLFSQLFSLEMLWLQHNAICHLPVSLFSSLHNLTFLSLKDNA
                     LRTLPEGLFAHNQGLLHLSLSYNQLETIPEGAFTNLSRLVSLTLSHNAITDLPEHVFR
                     NLEQLVKLSLDSNNLTALHPALFHNLSRLQLLNLSRNQLTTLPGGIFDTNYDLFNLAL
                     LGNPWQCDCHLSYLTSWLRLYNNQISNTHTFCAGPAYLKGQLVPNLKQEQLICPVNPG
                     HLSFRALGLDEGEPAGSWDLTVEGRAAHSQCAYSNPEGTVLLACEESRCRWLNIQLSS
                     RDGSDSAAMVYNSSQEWGLRSSCGLLRVTVSIEAPAAGP"
     regulatory      4559..4564
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Cpn2"
                     /gene_synonym="1300018K11Rik"
                     /note="hexamer: ACTAAA"
     polyA_site      4583
                     /gene="Cpn2"
                     /gene_synonym="1300018K11Rik"
                     /note="major polyA site"
ORIGIN      
        1 cacagagcac atgaaattct cctctgtttc cgaggaaaaa ccagggcttc tggccccacc
       61 tgcccagccc ctcacgacga tgttcccggg agcgtggttg tgctgggtct cactcctgct
      121 cctggcgagg ctcacccagc cttgccccgt gggctgcgac tgcttcggca gggaggtctt
      181 ctgctcagac gagcagctgg ccgacatccc tccggacatc cccccacaca tcacagacat
      241 tgtctttgtg gagactgcct tcaccacggt gaggaccagg gctttcagcg gtagccccaa
      301 cctgaccaag gtggtcttcc tcaacactca ggtccgtcac ctggagcccg atgcctttgg
      361 aggactgccc aggctgcagg acctggagat cacgggcagc cccgtctcca acctcagcgc
      421 ccacatcttt tctaacctga gctcactgga gaagctcacc ctggactttg acaggctggc
      481 gggtctgcct gaggatctct tttgccacat ggacatcctg gagtccctcc aacttcaagg
      541 aaaccaactc cgaaccttgc ccgggagact attccagtct ctgagggatc tgagaaccct
      601 caacctggct cagaacctcc tgacacagct gcctaagggg gcgttccagt cactcactgg
      661 cctgcagatg ttgaagctga gtaacaatat gcttgccaga ctccccgagg gagcgctggg
      721 cagtctgagc agcctgcaag agctcttcct ggatggcaat gccatcacag agctgtcccc
      781 acatctgttc tcacagctgt tcagcctaga gatgctatgg ctgcagcaca atgccatctg
      841 ccacctgccg gtctctctct tctcctccct gcacaatctg acctttctaa gcctgaagga
      901 caacgcactg cggactctgc ccgagggtct ctttgcccac aaccagggcc tccttcacct
      961 gtctctttcc tacaaccagc tggagaccat ccccgagggg gccttcacca acctgtcacg
     1021 tcttgtttcc ctcacgctct cgcacaatgc catcactgac cttcccgagc atgttttcag
     1081 gaacctcgag cagttggtta aactctctct ggacagcaat aacttgacgg ccctgcaccc
     1141 ggctctcttt cacaatctgt ccaggcttca gctcctcaac ttgtccagga accagctgac
     1201 cacgctcccc gggggcatct ttgacaccaa ctacgacctc ttcaacctgg ctttgcttgg
     1261 caacccctgg cagtgcgact gccatctgtc ctatctcacc agttggctcc gtctttacaa
     1321 caaccagatc tccaacaccc atactttctg tgcaggcccc gcatacctca agggccagtt
     1381 ggtgccaaac ttgaagcagg agcagctgat atgcccggtc aacccaggcc acttgagctt
     1441 cagggccctg gggctagatg agggggagcc agcgggcagt tgggatctga ctgtggaggg
     1501 gagggcagct cacagccagt gtgcctacag caaccctgag ggcactgtgc tgcttgcctg
     1561 tgaggagagc cggtgtcgct ggctgaacat ccagctgtct tcccgggacg gctcagactc
     1621 tgcagcaatg gtctacaatt ccagtcagga gtgggggctg aggtcaagct gcggcctcct
     1681 aagggtcact gtgtctattg aggctccggc tgctggccct taggtgcagc agattcggaa
     1741 gcctcagggc agaggggaga gaatgtctga aaccaatata agggacatgg ccacagctcc
     1801 agggtaggga ggagagaggc ctgcttcacc tagggtctcc tcaacttctg tcaccatgct
     1861 ttcttggagc ctatggccta acaaggtcac cctcagatct tccaaggtac ccatgggaac
     1921 catggtctag cacagtggtt ctcgaccttt ccaatgttgt gaccctttaa tacggttcct
     1981 catgctgtgg tggcccccaa ccgtaaaatt atttcattgc aacttttata actgtaattt
     2041 tgctactatt tggatgataa tttttttttt ttggagatag gggtttggca aaggagtcat
     2101 gacctacagg tggagaacca ctggcctaga gattatacga cacccccaac cccaccccca
     2161 tcacggcaca gcgttttcct attgttaagc cccaccccgc tccctgctct gagttctagc
     2221 tttgaggtgt gttcacctct actgtactta tacaaagtag aggccgtgtc cctttaatag
     2281 atcaaaagat caatagtctg ttctgatctc ttggcctcaa ttttgttttc aatggctaga
     2341 gagaggaaga gggtgtccaa ggatctgagg tgattggaaa agggcagggc ggggctgtgt
     2401 ggggaaggga gattgtcttg gctgtctctc aagccgtgtc caggagaaac tgaggcaaag
     2461 ccaggctgtg tggctctcag gagggcagtg gcattgccct cttcagcaca caagatgtct
     2521 gttccctgag gatgaggaag aggaggggta gggggagggg aagagggggg gaggggaaga
     2581 ggaaggggag gaggagggga gctggtggac ttaggtagct gctttgtgtc atggctttca
     2641 gggcatctga ttctgggctg ctcagtcact gaacagctct tctctagttt ctaagatgtc
     2701 tctggtggcc ctcctcagcc accactcact ggatggcccc tcagcactga tggcctcata
     2761 tctgtggctt aggctcagca cttcctccat tcaattagcc ccagcgcatc tagacatcta
     2821 atcaactctt gattcccacc ttctgtcctt gcaaccccct gttctcgaac cttcccgttc
     2881 cccatcctca ggctaaggaa tggctctctt tcatcttccc tttcttcttt ctcacttccc
     2941 caagccccct agaacgttgc acaacctcct acttggtttc agggctgcag agtagaaagg
     3001 ccgagtgaga tgcatggtgc cccaccccca ccgcagcagc tcatagccca ggatggacag
     3061 gagtacgtgg gaggtacctc caaacacaga ggtccatgcg tgggcgtgcc aaggccctgg
     3121 cgctcaggag gtatcctagt tatgtttctg ttgtgataaa acactgagca aactgagcca
     3181 acttgggggg cagagatggt ttatttggct aatgagtcac agtccatctt tgtgggaact
     3241 cagggcagga agtcaaggca ggactggaag tagaaacgag gggcactgtt tgttgctttc
     3301 aggcttatct tcagctgcct ttctaatacc ttgcaggacc acctgcccag gagtggtgct
     3361 gtccacagtg ggtggggccc tcccactcca ccaatcatta atcaataaaa agccctctgc
     3421 tgggtgtagt ggcacacacc tttaatccca gcacctggga ggcagaagca agtggatgtc
     3481 tgtgagttca aggctagcct ggtttacgtc gagagctcca ggctagccag aactacacag
     3541 caagactctg tctcaccatt aataatgata acaacaataa ataaataatt aaaaggtccc
     3601 tattggctaa tctgatagaa gcatcttttc aactgaggtt ctctttcccg atgcccttcg
     3661 cttgtccaag ttgacaaaac taaccagcac aggaggcctg gggaatccct ggccttgggg
     3721 aagctacagg ctgaaacaga gtgggagtta catgctggag acatgcaagc cgggaggact
     3781 gggcaacaca ctgcagaaca ggcccccgag tcgggagagc agaggggaag gtcaaggctt
     3841 ggagcccagc ctccaggtct cccctgcctc tagggtaggc tgtggtgatg aggagtatgg
     3901 gaactgaagc tgggccctct acaaaggcac ccttctgctg agccatctct ccagcccact
     3961 tggaggaatc tgtgcttgtt aaggactctg caggcagttc acagaggcag gtaggactcg
     4021 ctccagctta tggttcaagc agttggccca gaacatgaag ctagggcaac agcttgagct
     4081 agggtttggc tagagatgac aaccgacagg caagggatgc taagtagttt aacagcactt
     4141 acacaggtgg attaagagcc ccgatggtga attacagaaa cgcaaacgaa cctagaatag
     4201 gttctgtgtt acatctattc tataggaagc tcatatggag tggtgtaaca gggtccagac
     4261 cttagcacag ctgactccat gatggaagtg gcattccgac tgtaaaactc agcacataga
     4321 caccgccacc agccagaatg aaaacaaaga tctaatccac caaggtccac agttctaaga
     4381 aagtctccac atgaactaac cttgcttttt ggcttctgta attatgcttc tggctaactg
     4441 ttcttgctaa ctgaagaatg tcaacccaga atacggtttt cgtccttaaa agctcattca
     4501 gagagaggct cagggctacg ctggcatctg gaatacccag tgtagtagcc agccagctac
     4561 taaagacttt cttttggctt aaa
//
