LOCUS       NM_145493               1767 bp    mRNA    linear   ROD 10-FEB-2025
DEFINITION  Mus musculus complexin 4 (Cplx4), mRNA.
ACCESSION   NM_145493 XM_355097
VERSION     NM_145493.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1767)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 1767)
  AUTHORS   Lux,U.T., Meyer,J., Jahn,O., Davison,A., Babai,N., Giessl,A.,
            Wartenberg,A., Sticht,H., Brose,N., Reim,K. and Brandstatter,J.H.
  TITLE     Light-dependent regulation of neurotransmitter release from rod
            photoreceptor ribbon synapses involves an interplay of Complexin 4
            and Transducin with the SNARE complex
  JOURNAL   Front Mol Neurosci 17, 1308466 (2024)
   PUBMED   38481472
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 1767)
  AUTHORS   Babai,N., Sendelbeck,A., Regus-Leidig,H., Fuchs,M., Mertins,J.,
            Reim,K., Brose,N., Feigenspan,A. and Brandstatter,J.H.
  TITLE     Functional Roles of Complexin 3 and Complexin 4 at Mouse
            Photoreceptor Ribbon Synapses
  JOURNAL   J Neurosci 36 (25), 6651-6667 (2016)
   PUBMED   27335398
  REMARK    GeneRIF: The findings of this study reveal a suppressing action of
            Cplx3/4 on tonic neurotransmitter release, a facilitating action on
            evoked release, and a regulatory role of Cplx3/4 in the
            adaptation-dependent availability of synaptic vesicles at mouse
            photoreceptor ribbon synapses.
            Erratum:[J Neurosci. 2017 Aug 9;37(32):7796. doi:
            10.1523/JNEUROSCI.1954-17.2017.. PMID: 31305591]
REFERENCE   4  (bases 1 to 1767)
  AUTHORS   Kurokawa,A., Narukawa,M., Ohmoto,M., Yoshimoto,J., Abe,K. and
            Misaka,T.
  TITLE     Expression of the synaptic exocytosis-regulating molecule complexin
            2 in taste buds and its participation in peripheral taste
            transduction
  JOURNAL   J Neurochem 133 (6), 806-814 (2015)
   PUBMED   25692331
REFERENCE   5  (bases 1 to 1767)
  AUTHORS   Landgraf,I., Muhlhans,J., Dedek,K., Reim,K., Brandstatter,J.H. and
            Ammermuller,J.
  TITLE     The absence of Complexin 3 and Complexin 4 differentially impacts
            the ON and OFF pathways in mouse retina
  JOURNAL   Eur J Neurosci 36 (4), 2470-2481 (2012)
   PUBMED   22694764
  REMARK    GeneRIF: The lack of Cplx 3 and Cplx 4 differentially impacts the
            ON and OFF pathways in the retina is corroborated by localization
            studies of Cplx 3 and Cplx 4 in the retina.
REFERENCE   6  (bases 1 to 1767)
  AUTHORS   Strenzke,N., Chanda,S., Kopp-Scheinpflug,C., Khimich,D., Reim,K.,
            Bulankina,A.V., Neef,A., Wolf,F., Brose,N., Xu-Friedman,M.A. and
            Moser,T.
  TITLE     Complexin-I is required for high-fidelity transmission at the
            endbulb of Held auditory synapse
  JOURNAL   J Neurosci 29 (25), 7991-8004 (2009)
   PUBMED   19553439
REFERENCE   7  (bases 1 to 1767)
  AUTHORS   Reim,K., Regus-Leidig,H., Ammermuller,J., El-Kordi,A.,
            Radyushkin,K., Ehrenreich,H., Brandstatter,J.H. and Brose,N.
  TITLE     Aberrant function and structure of retinal ribbon synapses in the
            absence of complexin 3 and complexin 4
  JOURNAL   J Cell Sci 122 (Pt 9), 1352-1361 (2009)
   PUBMED   19386896
  REMARK    GeneRIF: Results show that complexin 3 and complexin 4 are
            essential regulators of transmitter release at retinal ribbon
            synapses.
REFERENCE   8  (bases 1 to 1767)
  AUTHORS   Glynn,D., Drew,C.J., Reim,K., Brose,N. and Morton,A.J.
  TITLE     Profound ataxia in complexin I knockout mice masks a complex
            phenotype that includes exploratory and habituation deficits
  JOURNAL   Hum Mol Genet 14 (16), 2369-2385 (2005)
   PUBMED   16000319
  REMARK    GeneRIF: Disruption in complexin I is associated with profound
            ataxia with a complex phenotype that includes exploratory and
            habituation deficits
REFERENCE   9  (bases 1 to 1767)
  AUTHORS   Reim,K., Wegmeyer,H., Brandstatter,J.H., Xue,M., Rosenmund,C.,
            Dresbach,T., Hofmann,K. and Brose,N.
  TITLE     Structurally and functionally unique complexins at retinal ribbon
            synapses
  JOURNAL   J Cell Biol 169 (4), 669-680 (2005)
   PUBMED   15911881
REFERENCE   10 (bases 1 to 1767)
  AUTHORS   Blackshaw,S., Harpavat,S., Trimarchi,J., Cai,L., Huang,H.,
            Kuo,W.P., Weber,G., Lee,K., Fraioli,R.E., Cho,S.H., Yung,R.,
            Asch,E., Ohno-Machado,L., Wong,W.H. and Cepko,C.L.
  TITLE     Genomic analysis of mouse retinal development
  JOURNAL   PLoS Biol 2 (9), E247 (2004)
   PUBMED   15226823
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from BC016082.1.
            
            On Dec 17, 2003 this sequence version replaced XM_355097.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC016082.1, AK044262.1 [ECO:0000332]
            RNAseq introns              :: partial sample support SAMN00849384,
                                           SAMN01164131 [ECO:0000350]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..1767
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10090"
                     /chromosome="18"
                     /map="18 39.05 cM"
     gene            1..1767
                     /gene="Cplx4"
                     /gene_synonym="A930004D23Rik; CPXIV; Gm957"
                     /note="complexin 4"
                     /db_xref="GeneID:225644"
                     /db_xref="MGI:MGI:2685803"
     exon            1..298
                     /gene="Cplx4"
                     /gene_synonym="A930004D23Rik; CPXIV; Gm957"
                     /inference="alignment:Splign:2.1.0"
     CDS             132..614
                     /gene="Cplx4"
                     /gene_synonym="A930004D23Rik; CPXIV; Gm957"
                     /note="complexin IV; CPX IV"
                     /codon_start=1
                     /product="complexin-4"
                     /protein_id="NP_663468.1"
                     /db_xref="CCDS:CCDS29312.1"
                     /db_xref="GeneID:225644"
                     /db_xref="MGI:MGI:2685803"
                     /translation="MAFFVKNMISNQVKNLGFGGGSEEKKEEGGTSDPAAAKGMTREE
                     YEEYQKQMIEEKMERDAAFTQKKAERACLRVHLRDKYRLPKSEMDETQIQLAGDDVDL
                     PEDLRKMVDEDQDEEEEKDSILGQLQNLQNMDLDTIKEKAQATFTEIKQSAEQKCSVM
                     "
     exon            299..386
                     /gene="Cplx4"
                     /gene_synonym="A930004D23Rik; CPXIV; Gm957"
                     /inference="alignment:Splign:2.1.0"
     exon            387..1756
                     /gene="Cplx4"
                     /gene_synonym="A930004D23Rik; CPXIV; Gm957"
                     /inference="alignment:Splign:2.1.0"
     regulatory      687..692
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Cplx4"
                     /gene_synonym="A930004D23Rik; CPXIV; Gm957"
                     /note="hexamer: AATATA"
     polyA_site      708
                     /gene="Cplx4"
                     /gene_synonym="A930004D23Rik; CPXIV; Gm957"
                     /note="major polyA site"
     regulatory      1730..1735
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Cplx4"
                     /gene_synonym="A930004D23Rik; CPXIV; Gm957"
                     /note="hexamer: AATAAA"
     polyA_site      1756
                     /gene="Cplx4"
                     /gene_synonym="A930004D23Rik; CPXIV; Gm957"
ORIGIN      
        1 cgcaaatcct tcgctcccaa atgtgtggct gtggttgaaa atatttggag gttttgcttt
       61 ggtttggttg gggtatattt tttttttttt gtcgatcttt gacagggttt cagttctcca
      121 aggccgagaa aatggctttc tttgtgaaaa atatgataag taaccaggta aagaatttag
      181 gatttggagg tgggtctgaa gaaaagaaag aagaaggagg cacctcagac cccgcggcgg
      241 ctaaagggat gactagagag gaatacgagg aataccagaa gcagatgatt gaagagaaga
      301 tggagagaga tgccgcattt acgcagaaaa aggcagagag ggcgtgccta cgagtccacc
      361 tcagggacaa gtacaggctt ccaaagagtg aaatggatga gacacaaatc caactggctg
      421 gagacgatgt ggacttacca gaagatctcc gaaagatggt agatgaagac caagatgagg
      481 aagaggagaa ggattctatc cttgggcagc tgcagaacct ccaaaacatg gacttggata
      541 ccattaaaga aaaggcacaa gccaccttca cagaaatcaa gcagtcagca gagcagaagt
      601 gttctgtgat gtgagagggg cagactgcct tcaggaagac ggggctcctg ggggtgggca
      661 tgaccagcac gcattataac ttagcaaata tatctaggga aaccatgatg ctgaggctgt
      721 ctactaccgg catgtagaca ataactcggt cgatcttgat gtcccaggta cggcactaac
      781 cagcccacac atcacgggca cggaacatgt ttccctttag ttatagccac aaataattaa
      841 ggaatatcag ccataagtta tgatgtggga ccatctggtt aaagatcttt aggacagcca
      901 gaaggagtca aacaaaagca tttcctttcc agctttcttc cttatcgagc aggtcccaac
      961 catcagcgtg attcgttacc tctagaggaa aggctgctcg cctggtacca agactacaga
     1021 caggtgacag agcaatcaga tgtcacgttg ctttttactt gcagcatcct tgatatccat
     1081 gatttttttc cccttaaaac cggctctcag acactgtgca agtcattttg tgtttctgca
     1141 atcaaatatc tgatatacac atcataaatg gaagaaagat ttatgcgggg gtcatggttc
     1201 agcaggtcca gtccatggtt gcttggctgt atgggattgg gcagaacccc atggcaggga
     1261 ggatagaggg tggagtggca tccaggaaca agaaatgcac agtcatctac tatccttaag
     1321 ttaggtccta cctgcgatcc tttaatacag ttcctcacgt gggggtgacc ctccccccag
     1381 ccacaaaatt attttctctg ctatttcata actgtaattt ttctagtgtt atgggttgta
     1441 atataagtat ctgtgttttc tggtggtctt aggcgacctc tgtgaaagga ttgagaacag
     1501 ctaccctggt acatcgcacc agatgactct atctcagtta acaccaactc ctgagaatat
     1561 gggctggggc agggacgatg tgtatattgg gtcagaaatt ctcaaacatt cttttgtggc
     1621 ccctttcact tgagaaattt tcacacactc tggtgtatat atgtgaatat tcactgacaa
     1681 gtcataaaaa aactgaaaca actttacatc taattttagc atttattaaa ataaaaggaa
     1741 atgtgtattc taagaaaaaa aaaaaaa
//
