LOCUS       NM_013494               2183 bp    mRNA    linear   ROD 07-OCT-2024
DEFINITION  Mus musculus carboxypeptidase E (Cpe), mRNA.
ACCESSION   NM_013494 XM_911185
VERSION     NM_013494.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2183)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2183)
  AUTHORS   Fulcher,J.M., Swensen,A.C., Chen,Y.C., Verchere,C.B., Petyuk,V.A.
            and Qian,W.J.
  TITLE     Top-Down Proteomics of Mouse Islets With Beta Cell CPE Deletion
            Reveals Molecular Details in Prohormone Processing
  JOURNAL   Endocrinology 164 (12) (2023)
   PUBMED   37967211
  REMARK    GeneRIF: Top-Down Proteomics of Mouse Islets With Beta Cell CPE
            Deletion Reveals Molecular Details in Prohormone Processing.
REFERENCE   3  (bases 1 to 2183)
  AUTHORS   Bohuslavova,R., Fabriciova,V., Smolik,O., Lebron-Mora,L.,
            Abaffy,P., Benesova,S., Zucha,D., Valihrach,L., Berkova,Z.,
            Saudek,F. and Pavlinkova,G.
  TITLE     NEUROD1 reinforces endocrine cell fate acquisition in pancreatic
            development
  JOURNAL   Nat Commun 14 (1), 5554 (2023)
   PUBMED   37689751
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2183)
  AUTHORS   Chen,Y.C., Taylor,A.J., Fulcher,J.M., Swensen,A.C., Dai,X.Q.,
            Komba,M., Wrightson,K.L.C., Fok,K., Patterson,A.E., Klein
            Geltink,R.I., MacDonald,P.E., Qian,W.J. and Verchere,C.B.
  TITLE     Deletion of Carboxypeptidase E in beta-Cells Disrupts Proinsulin
            Processing but Does Not Lead to Spontaneous Development of Diabetes
            in Mice
  JOURNAL   Diabetes 72 (9), 1277-1288 (2023)
   PUBMED   37364047
  REMARK    GeneRIF: Deletion of Carboxypeptidase E in beta-Cells Disrupts
            Proinsulin Processing but Does Not Lead to Spontaneous Development
            of Diabetes in Mice.
REFERENCE   5  (bases 1 to 2183)
  AUTHORS   Lu,C., Zhang,J., Wang,B., Gao,Q., Ma,K., Pei,S., Li,J. and Cui,S.
  TITLE     Casein kinase 1alpha is required to maintain murine hypothalamic
            pro-opiomelanocortin expression
  JOURNAL   iScience 26 (5), 106670 (2023)
   PUBMED   37168577
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2183)
  AUTHORS   Naggert,J.K., Fricker,L.D., Varlamov,O., Nishina,P.M., Rouille,Y.,
            Steiner,D.F., Carroll,R.J., Paigen,B.J. and Leiter,E.H.
  TITLE     Hyperproinsulinaemia in obese fat/fat mice associated with a
            carboxypeptidase E mutation which reduces enzyme activity
  JOURNAL   Nat Genet 10 (2), 135-142 (1995)
   PUBMED   7663508
REFERENCE   7  (bases 1 to 2183)
  AUTHORS   Keightley,P.D.
  TITLE     Chewing the fat
  JOURNAL   Nat Genet 10 (2), 125-126 (1995)
   PUBMED   7663503
REFERENCE   8  (bases 1 to 2183)
  AUTHORS   Kingsmore,S.F., Suh,D. and Seldin,M.F.
  TITLE     Genetic mapping of the glycine receptor alpha 3 subunit on mouse
            chromosome 8
  JOURNAL   Mamm Genome 5 (12), 831-832 (1994)
   PUBMED   7894176
REFERENCE   9  (bases 1 to 2183)
  AUTHORS   Mysore,J.V., Duhamel,G.E. and Mathiesen,M.R.
  TITLE     Morphologic analysis of enteric lesions in conventional and
            streptomycin-treated inbred C3H/HeN mice infected with Serpulina
            (Treponema) hyodysenteriae
  JOURNAL   Lab Anim Sci 42 (1), 7-12 (1992)
   PUBMED   1316514
REFERENCE   10 (bases 1 to 2183)
  AUTHORS   Coleman,D.L. and Eicher,E.M.
  TITLE     Fat (fat) and tubby (tub): two autosomal recessive mutations
            causing obesity syndromes in the mouse
  JOURNAL   J Hered 81 (6), 424-427 (1990)
   PUBMED   2250094
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from CX241703.1, AK047891.1 and
            BE849320.1.
            
            On Jan 26, 2016 this sequence version replaced NM_013494.3.
            
            Summary: This gene encodes carboxypeptidase E, a
            prohormone-processing exopeptidase found in secretory granules of
            endocrine and neuroendocrine cells. The encoded preproprotein
            undergoes proteolytic processing to generate a mature, functional
            enzyme that cleaves the C-terminal basic residues of protein
            substrates. A missense mutation in this gene is responsible for the
            obesity phenotype in a mouse model known as the 'fat mouse.' Mice
            lacking the functional product of this gene exhibit impaired
            processing of multiple peptide hormones such as proinsulin,
            prodynorphin, proneurotensin, promelanin-concentrating hormone and
            pro-opiomelanocortin. [provided by RefSeq, Jan 2016].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC010197.1, SRR1660811.112356.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849382, SAMN01164131
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-78                CX241703.1         1-78
            79-2156             AK047891.1         1-2078
            2157-2183           BE849320.1         413-439
FEATURES             Location/Qualifiers
     source          1..2183
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="8"
                     /map="8 32.3 cM"
     gene            1..2183
                     /gene="Cpe"
                     /gene_synonym="CPH; Cph-1; Cph1; fat; NF-alpha1"
                     /note="carboxypeptidase E"
                     /db_xref="GeneID:12876"
                     /db_xref="MGI:MGI:101932"
     exon            1..467
                     /gene="Cpe"
                     /gene_synonym="CPH; Cph-1; Cph1; fat; NF-alpha1"
                     /inference="alignment:Splign:2.1.0"
     CDS             161..1591
                     /gene="Cpe"
                     /gene_synonym="CPH; Cph-1; Cph1; fat; NF-alpha1"
                     /EC_number="3.4.17.10"
                     /note="enkephalin convertase; prohormone-processing
                     carboxypeptidase; carboxypeptidase H"
                     /codon_start=1
                     /product="carboxypeptidase E preproprotein"
                     /protein_id="NP_038522.2"
                     /db_xref="CCDS:CCDS22327.1"
                     /db_xref="GeneID:12876"
                     /db_xref="MGI:MGI:101932"
                     /translation="MAGRGGRVLLALCAALVAGGWLLTAEAQEPGAPAAGMRRRRRLQ
                     QEDGISFEYHRYPELREALVSVWLQCTAISRIYTVGRSFEGRELLVIELSDNPGVHEP
                     GEPEFKYIGNMHGNEAVGRELLIFLAQYLCNEYQKGNETIVNLIHSTRIHIMPSLNPD
                     GFEKAASQPGELKDWFVGRSNAQGIDLNRNFPDLDRIVYVNEKEGGPNNHLLKNLKKI
                     VDQNSKLAPETKAVIHWIMDIPFVLSANLHGGDLVANYPYDETRSGTAHEYSSCPDDA
                     IFQSLARAYSSFNPVMSDPNRPPCRKNDDDSSFVDGTTNGGAWYSVPGGMQDFNYLSS
                     NCFEITVELSCEKFPPEETLKSYWEDNKNSLISYLEQIHRGVKGFVRDLQGNPIANAT
                     ISVDGIDHDVTSAKDGDYWRLLAPGNYKLTASAPGYLAITKKVAVPFSPAVGVDFELE
                     SFSERKEEEKEELMEWWKMMSETLNF"
     exon            468..664
                     /gene="Cpe"
                     /gene_synonym="CPH; Cph-1; Cph1; fat; NF-alpha1"
                     /inference="alignment:Splign:2.1.0"
     exon            665..832
                     /gene="Cpe"
                     /gene_synonym="CPH; Cph-1; Cph1; fat; NF-alpha1"
                     /inference="alignment:Splign:2.1.0"
     exon            833..950
                     /gene="Cpe"
                     /gene_synonym="CPH; Cph-1; Cph1; fat; NF-alpha1"
                     /inference="alignment:Splign:2.1.0"
     exon            951..1133
                     /gene="Cpe"
                     /gene_synonym="CPH; Cph-1; Cph1; fat; NF-alpha1"
                     /inference="alignment:Splign:2.1.0"
     exon            1134..1273
                     /gene="Cpe"
                     /gene_synonym="CPH; Cph-1; Cph1; fat; NF-alpha1"
                     /inference="alignment:Splign:2.1.0"
     exon            1274..1373
                     /gene="Cpe"
                     /gene_synonym="CPH; Cph-1; Cph1; fat; NF-alpha1"
                     /inference="alignment:Splign:2.1.0"
     exon            1374..1492
                     /gene="Cpe"
                     /gene_synonym="CPH; Cph-1; Cph1; fat; NF-alpha1"
                     /inference="alignment:Splign:2.1.0"
     exon            1493..2172
                     /gene="Cpe"
                     /gene_synonym="CPH; Cph-1; Cph1; fat; NF-alpha1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2025..2030
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Cpe"
                     /gene_synonym="CPH; Cph-1; Cph1; fat; NF-alpha1"
                     /note="hexamer: AATGAA"
     polyA_site      2056
                     /gene="Cpe"
                     /gene_synonym="CPH; Cph-1; Cph1; fat; NF-alpha1"
                     /note="major polyA site"
ORIGIN      
        1 cgcagagccg tcgcgtcgcc gctgtcctct gggtggcccc agagcgcacc gttcattgag
       61 cggtggaagg tgaggcgaga ggaggctggt gctgagctcg ccaactccac ccgggcccgg
      121 gcagacaaaa gaggccggca gtgcagctcg cggggacgcg atggccgggc gcggaggacg
      181 ggtgctgctg gcgctgtgtg ccgcgctggt ggccggcggg tggctgctga cggctgaagc
      241 ccaggagccc ggggcgccag cggctggcat gaggcgccgc cggcggctcc agcaagagga
      301 cggcatctcc ttcgagtacc accgctatcc agagctgcgc gaggcgctgg tgtccgtatg
      361 gctgcagtgc accgccatca gcagaatcta cacagtgggg cgcagcttcg agggccggga
      421 gctcctggtc atcgagctgt ctgacaaccc cggggtccat gagccgggtg aacctgaatt
      481 taaatacatt gggaacatgc atggtaatga ggcggttgga cgggaactgc ttattttctt
      541 ggcccagtac ctgtgtaacg agtaccagaa aggcaatgag acaattgtca acctgatcca
      601 cagcacccga attcatatca tgccctcctt gaaccccgac ggctttgaga aagccgcatc
      661 gcagcccggc gagctgaagg actggtttgt gggccgcagc aacgcccagg gaatagatct
      721 gaaccgtaac ttcccagacc tggacaggat cgtgtatgtt aatgagaaag aaggcggtcc
      781 taacaatcac ctgctgaaga atctgaagaa aattgtggac caaaattcaa agcttgcccc
      841 cgagaccaag gctgtcattc actggatcat ggacattcca tttgtgcttt ctgccaatct
      901 gcacggagga gaccttgtgg ctaattaccc atatgatgag acacggagcg gtactgctca
      961 cgaatacagt tcctgccctg atgacgcaat tttccaaagc ttggctcgcg cgtactcttc
     1021 tttcaaccca gtcatgtctg accccaatcg acctccctgt cgcaagaatg acgatgacag
     1081 cagctttgta gatggaacga ccaatggtgg tgcatggtac agcgtccccg gtggaatgca
     1141 agacttcaat tacctgagca gcaactgctt cgagatcact gtggagctta gctgtgagaa
     1201 gttcccaccg gaagagactc tcaaaagcta ctgggaagat aacaaaaact ccctcatcag
     1261 ctacctggag cagatacacc gaggtgttaa agggtttgtc cgtgaccttc agggtaaccc
     1321 gattgccaac gcaaccatct ctgtggacgg gatagaccat gatgtcacct cggctaagga
     1381 tggggattac tggcgattgc ttgctcctgg aaactataaa cttacagcct ccgctcctgg
     1441 ctacctggca atcacaaaga aagtggcagt tccttttagc cctgctgttg gggtggactt
     1501 tgagcttgag tctttctctg aaaggaagga ggaggagaag gaagaattga tggagtggtg
     1561 gaaaatgatg tcagaaactt tgaattttta agaaaggctt ctaactaatt gctttaatct
     1621 atctatagac tgtagtaaga tgcaatgtgg ctcttttctt ttaggttgtg tgcagttgat
     1681 atttaacatt gatttatttt tgatcattta agtaatagtt agtaatcacg taaatacacc
     1741 cggacagaaa tataatgtct ggatctactt cattcttaca tcaacattca ctttaaaatc
     1801 tatcgaagct cttttaacgt aatgggtgac aatgtcacat gacagatgcc atgaagaagt
     1861 caaccgatat agcttggatc tgtgaaccct gtactgcgag aatcacatag ttccatataa
     1921 gttgtcctta gtctcttgtg ctgattcact gtataagcat gatcctggta atgcactttg
     1981 gatgggaaga aaatgtacgt gcttttcaga ggggctctga acagaatgaa aacctagttc
     2041 ttgcgtgtac tttgaagaat ggaattgtat tagtcagcct gttaatgcca cttcagagtt
     2101 tggggttttg tcttgattgt agattggccc agaattgcat tctgatgaat aaaggcaaaa
     2161 aaaaaaaaaa aaaaaaaaaa aaa
//
