LOCUS       NM_177834               1902 bp    mRNA    linear   ROD 11-MAR-2025
DEFINITION  Mus musculus carboxypeptidase A6 (Cpa6), transcript variant 1,
            mRNA.
ACCESSION   NM_177834
VERSION     NM_177834.5
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1902)
  AUTHORS   Hata,K., Wakamori,K., Hirakawa-Yamamura,A., Ichiyama-Kobayashi,S.,
            Yamaguchi,M., Okuzaki,D., Takahata,Y., Murakami,T., Uzawa,N.,
            Yamashiro,T. and Nishimura,R.
  TITLE     Serinc5 Regulates Sequential Chondrocyte Differentiation by
            Inhibiting Sox9 Function in Pre-Hypertrophic Chondrocytes
  JOURNAL   J Cell Physiol 240 (1), e31490 (2025)
   PUBMED   39568258
REFERENCE   2  (bases 1 to 1902)
  AUTHORS   Lyons,P.J., Callaway,M.B. and Fricker,L.D.
  TITLE     Characterization of carboxypeptidase A6, an extracellular matrix
            peptidase
  JOURNAL   J Biol Chem 283 (11), 7054-7063 (2008)
   PUBMED   18178555
  REMARK    GeneRIF: CPA6 may have a role in the regulation of neuropeptides in
            the extracellular environment within the olfactory bulb and other
            parts of the brain
REFERENCE   3  (bases 1 to 1902)
  AUTHORS   Fontenele-Neto,J.D., Kalinina,E., Feng,Y. and Fricker,L.D.
  TITLE     Identification and distribution of mouse carboxypeptidase A-6
  JOURNAL   Brain Res Mol Brain Res 137 (1-2), 132-142 (2005)
   PUBMED   15950771
  REMARK    GeneRIF: The distribution of Cpa6 suggests a specific role in a
            limited number of tissues, and it is possible that this role
            involves an aspect of cell migration.
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC102426.6, AC102469.7,
            AC162861.2 and AC102613.7.
            
            On Jan 4, 2023 this sequence version replaced NM_177834.4.
            
            Summary: This gene encodes a protein that belongs to the
            metallocarboxypeptidase family of proteins that catalyze the
            release of a C-terminal amino acid from the target protein. The
            encoded preproprotein undergoes proteolytic cleavage to yield the
            mature form which is thought to play a role in cell migration. In
            humans, this protein regulates neuropeptides in the brain and
            mutations in this gene are associated with a recessive familial
            form of febrile seizures and with temporal lobe epilepsy.
            Alternative splicing results in multiple transcript variants
            encoding different isoforms. [provided by RefSeq, Jan 2014].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            ##Evidence-Data-START##
            Transcript exon combination :: AY773477.1, BC119559.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164131 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-294               AC102426.6         101023-101316
            295-370             AC102469.7         35924-35999
            371-495             AC162861.2         141627-141751       c
            496-610             AC162861.2         136751-136865       c
            611-712             AC162861.2         133816-133917       c
            713-817             AC162861.2         129827-129931       c
            818-928             AC102613.7         48211-48321
            929-1019            AC102613.7         49219-49309
            1020-1222           AC102613.7         120109-120311
            1223-1307           AC102613.7         128021-128105
            1308-1902           AC102613.7         132267-132861
FEATURES             Location/Qualifiers
     source          1..1902
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="1"
                     /map="1 2.45 cM"
     gene            1..1902
                     /gene="Cpa6"
                     /gene_synonym="9030616D13Rik"
                     /note="carboxypeptidase A6"
                     /db_xref="GeneID:329093"
                     /db_xref="MGI:MGI:3045348"
     exon            1..294
                     /gene="Cpa6"
                     /gene_synonym="9030616D13Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             179..1495
                     /gene="Cpa6"
                     /gene_synonym="9030616D13Rik"
                     /EC_number="3.4.17.1"
                     /note="isoform 1 preproprotein is encoded by transcript
                     variant 1; metallocarboxypeptidase"
                     /codon_start=1
                     /product="carboxypeptidase A6 isoform 1 preproprotein"
                     /protein_id="NP_808502.2"
                     /db_xref="CCDS:CCDS14819.1"
                     /db_xref="GeneID:329093"
                     /db_xref="MGI:MGI:3045348"
                     /translation="MNFLGNPRSHTAAFLPVCWLLLNILKPGHCHSYDNRYAGDKVIR
                     LIPKSEEEALALKNIYHQLKVDLWQPSSISYVSEGTITDVHISQNASRTLLAFLQETH
                     IYYKVLIEDLQKAVENENSLQTQRNRRSLSEYNYEVYHSLEDIQSWLHHLNQTQPGLV
                     RVFSIGRSYEGRPLFIMQLGRKSRAYKRAVWIDCGIHAREWIGPAFCQWFVREAILTY
                     KTDPAMKKMLNHLYFYIMPVFNVDGYHFSWTHDRFWRKTRSRDSKFRCRGVDANRNWK
                     VKWCDEGASAHPCDDTYCGPFPESEPEVKAVANFLRKHRKRIRAYLSFHAYAQMLLYP
                     YSYKYATIPNFSCVEFAAHKAVKALRSVHGIRYRHGPASQTLYVSSGNSMDWAYKNGI
                     PYAFAFELRDTGHFGFLLPEMLIKPTCTETMLAVKNITMHLLKKCP"
     exon            295..370
                     /gene="Cpa6"
                     /gene_synonym="9030616D13Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            371..495
                     /gene="Cpa6"
                     /gene_synonym="9030616D13Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            496..610
                     /gene="Cpa6"
                     /gene_synonym="9030616D13Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            611..712
                     /gene="Cpa6"
                     /gene_synonym="9030616D13Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            713..817
                     /gene="Cpa6"
                     /gene_synonym="9030616D13Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            818..928
                     /gene="Cpa6"
                     /gene_synonym="9030616D13Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            929..1019
                     /gene="Cpa6"
                     /gene_synonym="9030616D13Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1020..1222
                     /gene="Cpa6"
                     /gene_synonym="9030616D13Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1223..1307
                     /gene="Cpa6"
                     /gene_synonym="9030616D13Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1308..1902
                     /gene="Cpa6"
                     /gene_synonym="9030616D13Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1878..1883
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Cpa6"
                     /gene_synonym="9030616D13Rik"
                     /note="hexamer: AATACA"
     polyA_site      1902
                     /gene="Cpa6"
                     /gene_synonym="9030616D13Rik"
                     /note="major polyA site"
ORIGIN      
        1 agtcttaata gcaactccct agctgcctaa agtgtcacat tttgctcatt cccaagcctt
       61 ctcccccacc cctttcccag acaacttgct ggtgagcagg gggtgttgta agccacctcc
      121 agcctcttcc tttggtgact gctgttaagc aactttcagc tcctctcatt gcaatagtat
      181 gaacttcctc gggaatccca ggagccacac agcagctttt ctgccggttt gctggctcct
      241 tttgaatatt ctgaaacctg ggcactgcca ctcttacgac aaccgctatg ctggtgataa
      301 agtgataaga ctcatcccca aatccgaaga ggaagcactt gcactcaaga acatatacca
      361 ccaacttaag gtggacctgt ggcagcccag cagtatctcc tatgtgtcag aggggacaat
      421 tactgatgtc catatttccc aaaatgcttc cagaaccctg ttagcgttct tacaggaaac
      481 ccacatctac tacaaggtcc tcatagaaga tcttcagaaa gctgtggaaa atgaaaacag
      541 cttgcaaacc cagagaaaca gaagatccct gtctgaatac aactacgagg tgtatcactc
      601 cttagaagat attcagagtt ggttgcacca tctgaaccaa actcagccag gcctcgttcg
      661 agtgttctct attggaagat catacgaagg aagacctctc tttattatgc agctgggcag
      721 aaaatcacga gcctacaaaa gagctgtctg gatagactgc ggcattcacg caagagaatg
      781 gatcggtcct gccttttgtc agtggtttgt aagagaagcc attctgacct acaagactga
      841 cccagccatg aaaaaaatgt tgaatcatct gtatttctat atcatgcctg tattcaatgt
      901 cgatggatac catttcagct ggacacatga tagattctgg agaaagaccc ggtcaagaga
      961 ctctaagttt cgctgtcgtg gagttgatgc caatcgcaac tggaaagtga aatggtgcga
     1021 tgaaggagcc tccgcacacc cttgtgatga tacatactgt ggtcctttcc cagaatctga
     1081 accagaagtg aaggctgtag ctaacttcct tcgaaaacac agaaagcgca tcagggcgta
     1141 cctgtctttt catgcctatg ctcagatgtt gctatatcct tattcttaca aatatgcaac
     1201 aatccccaat tttagctgtg tggaatttgc agctcacaaa gctgtgaagg cacttcggtc
     1261 ggtacatggg atccgataca gacatggccc cgcctcccaa acactgtatg taagctctgg
     1321 taactcaatg gattgggctt ataaaaatgg aataccctat gcatttgctt ttgaactgcg
     1381 agacactggg cattttggat ttctactgcc agagatgctc attaaaccca cctgcacaga
     1441 gaccatgctg gctgtgaaga atatcacaat gcacctgcta aagaaatgtc cttgagagga
     1501 acagtcatct agtctaccaa cacagagaaa gcagattctg atcaaaaggc accttggcaa
     1561 acataactgt ttagactgga aggatccatg gacctaaaag gatctttcat gcaattcgac
     1621 actttgcaaa gactgaaaag tagggcacag cctactttgg tatgagaaga aagtccactt
     1681 tatatctctt tagagtccta tttgattaca agggggaggg aatgttttca aattcctttg
     1741 tctcaaaagg tgtacaaatt aattgaggat ttctcttctc tcaaccactc catccttgat
     1801 taagacctat gctaccaacg acattgaatt acttggttct tatttgaaat ggaaacaatc
     1861 aactgtgttt cttaataaat acataacttt tcaaaaaact aa
//
