LOCUS       NM_144537               2187 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus carboxypeptidase A5 (Cpa5), mRNA.
ACCESSION   NM_144537
VERSION     NM_144537.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2187)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2187)
  AUTHORS   Zhou,Y., Zhang,X., Xiong,S., Zeng,X. and Zhang,X.
  TITLE     Predicted gene 31453 (Gm31453) and the gene encoding
            carboxypeptidase A5 (Cpa5) are not essential for spermatogenesis
            and male fertility in the mouse
  JOURNAL   Reprod Fertil Dev 33 (6), 401-409 (2021)
   PUBMED   33745502
  REMARK    GeneRIF: Predicted gene 31453 (Gm31453) and the gene encoding
            carboxypeptidase A5 (Cpa5) are not essential for spermatogenesis
            and male fertility in the mouse.
REFERENCE   3  (bases 1 to 2187)
  AUTHORS   Hald,J., Hjorth,J.P., German,M.S., Madsen,O.D., Serup,P. and
            Jensen,J.
  TITLE     Activated Notch1 prevents differentiation of pancreatic acinar
            cells and attenuate endocrine development
  JOURNAL   Dev Biol 260 (2), 426-437 (2003)
   PUBMED   12921743
REFERENCE   4  (bases 1 to 2187)
  AUTHORS   Wei,S., Segura,S., Vendrell,J., Aviles,F.X., Lanoue,E., Day,R.,
            Feng,Y. and Fricker,L.D.
  TITLE     Identification and characterization of three members of the human
            metallocarboxypeptidase gene family
  JOURNAL   J Biol Chem 277 (17), 14954-14964 (2002)
   PUBMED   11836249
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC155656.10, AK015256.1 and
            BC138114.1.
            
            On Jul 19, 2016 this sequence version replaced NM_144537.3.
            
            Summary: This gene encodes a member of the carboxypeptidase A
            family of zinc metalloproteases. The encoded preproprotein
            undergoes proteolytic processing that removes the N-terminal
            activation peptide to generate a functional enzyme. This gene is
            expressed in mouse testes where the encoded protein is localized to
            the germ cells. This gene is located in a cluster of
            carboxypeptidase genes on chromosome 6. [provided by RefSeq, Jul
            2016].
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF466283.1, AK015256.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849384 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-276               AC155656.10        75774-76049
            277-482             AK015256.1         278-483
            483-1878            BC138114.1         1-1396
            1879-2187           AC155656.10        96201-96509
FEATURES             Location/Qualifiers
     source          1..2187
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="6"
                     /map="6 12.52 cM"
     gene            1..2187
                     /gene="Cpa5"
                     /gene_synonym="4930430M09Rik"
                     /note="carboxypeptidase A5"
                     /db_xref="GeneID:74649"
                     /db_xref="MGI:MGI:1921899"
     exon            1..434
                     /gene="Cpa5"
                     /gene_synonym="4930430M09Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            435..646
                     /gene="Cpa5"
                     /gene_synonym="4930430M09Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             531..1841
                     /gene="Cpa5"
                     /gene_synonym="4930430M09Rik"
                     /EC_number="3.4.17.1"
                     /codon_start=1
                     /product="carboxypeptidase A5 preproprotein"
                     /protein_id="NP_653120.1"
                     /db_xref="CCDS:CCDS19976.1"
                     /db_xref="GeneID:74649"
                     /db_xref="MGI:MGI:1921899"
                     /translation="MQGTQRGGLVPGLSPLDRRTLLFCNFILAVAWGQVNFTGDQVLR
                     VLAKNEKQLSLLRDLETQKPQKVDFWRGPARPSLPVDMRVPFSELPSVKAYLKSHGLA
                     YSIMIKDIQVLLDEERDAMAKSRRLERSTNSFSYSSYHTLDEIYSWIDNFVAEHSNLV
                     SKIHIGKSFENRSILVLKFSTGGPNRPAIWIDTGIHSREWITHATGIWISQKIVNAYG
                     KDHVLKRILNTMDIFIEIVTNPDGFAFTHSMNRLWRKNKSSQPGIFCIGVDLNRNWKA
                     GFGGNGSNKNPCSETYRGPAPESEPEVAAIVDFITGHGNFKAMISIHSYSQMVMYPYG
                     HSLEPVPNHEELFNLAKDAVKALNKVHGIQYIFGSISTTLYSASGISVDWAYDSGIKY
                     AFSFELRDTGQYGFLLPASQIVPTAEETWMALQTIMKHTLNHPY"
     exon            647..728
                     /gene="Cpa5"
                     /gene_synonym="4930430M09Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            729..863
                     /gene="Cpa5"
                     /gene_synonym="4930430M09Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            864..962
                     /gene="Cpa5"
                     /gene_synonym="4930430M09Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            963..1064
                     /gene="Cpa5"
                     /gene_synonym="4930430M09Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1065..1166
                     /gene="Cpa5"
                     /gene_synonym="4930430M09Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1167..1277
                     /gene="Cpa5"
                     /gene_synonym="4930430M09Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1278..1368
                     /gene="Cpa5"
                     /gene_synonym="4930430M09Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1369..1568
                     /gene="Cpa5"
                     /gene_synonym="4930430M09Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1569..1653
                     /gene="Cpa5"
                     /gene_synonym="4930430M09Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1654..2187
                     /gene="Cpa5"
                     /gene_synonym="4930430M09Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1941..1946
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Cpa5"
                     /gene_synonym="4930430M09Rik"
                     /note="hexamer: AATAGA"
     polyA_site      1963
                     /gene="Cpa5"
                     /gene_synonym="4930430M09Rik"
                     /note="major polyA site"
     regulatory      2150..2155
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Cpa5"
                     /gene_synonym="4930430M09Rik"
                     /note="hexamer: AATAAA"
     polyA_site      2185
                     /gene="Cpa5"
                     /gene_synonym="4930430M09Rik"
                     /note="major polyA site"
ORIGIN      
        1 tttctttcgc ctcgctcttg ttctcactct aattctctct cactcactct ctctctcttt
       61 ccatcttcca tttctaaacc aagtgcagga atagttgtct cattgttagt ggcttaaaat
      121 gatgcttttg aacatgagaa tgctgggtgc tttcagtgac tagtaccatc cccacctttt
      181 ttccttctga atttctgagc tattctttac cagctctctc ttttattctc atggctacta
      241 aagctgtaca attgctttct tttctaggtc cttaagccgt ttgctaatgt tactttctga
      301 gcatttccaa agctggggtt ctaggagagt ttggtgtgcc tgaacggctg acccatgaaa
      361 taaaggagta gggcaggcgg ggtggagagc agggccagct atcaatagaa gagtgacccc
      421 cctctccgca cacggcccgg gctgctggcc cttaggtgat gtgctgtcgt gaggcctagg
      481 ccatgcccaa gctggaggaa aacccttgac gcttgtctgg aaggagaagc atgcagggca
      541 cccaaagagg aggactagtc cctgggctgt cccctctgga cagacggaca cttctgttct
      601 gcaactttat cttggctgta gcttggggcc aagtgaattt cacaggagac caggttcttc
      661 gagtcctggc caaaaatgag aagcagctgt cacttctcag ggatctggag acccagaagc
      721 cccagaaggt ggacttctgg cgtggcccag ccagacctag cctccctgtg gacatgagag
      781 ttcccttctc tgaactgccc agtgttaaag cttatttgaa gtctcatggc cttgcctaca
      841 gcatcatgat aaaggacatc caggtgctac tggatgaaga gagagacgcc atggcaaagt
      901 ctcgcaggct ggaacgcagt accaacagct tcagctattc ctcttatcac accctggacg
      961 agatatatag ttggatcgac aactttgtag ctgaacattc taatcttgtc tccaaaattc
     1021 acattggcaa gagctttgaa aaccggtcca ttcttgtcct gaagttcagc actggaggtc
     1081 caaatcgccc ggctatctgg attgacactg ggattcattc cagagagtgg atcacccatg
     1141 ccaccggcat ctggatctcg cagaagattg tcaatgcata cggcaaagac catgtcttga
     1201 agaggatatt gaacactatg gacattttca tagagattgt cactaacccc gatggctttg
     1261 cttttactca tagcatgaac cgcttatggc gaaaaaacaa gtcaagtcaa cccggcatct
     1321 tctgcattgg cgtggacctc aacaggaact ggaaggcagg cttcggagga aatggttcta
     1381 acaaaaaccc ctgctcagag acttaccgag ggcctgcacc tgagtcagag ccagaggtag
     1441 ctgcaattgt ggacttcatc actggccatg ggaacttcaa agctatgatc tccatccaca
     1501 gctactctca gatggtcatg tatccttatg gccattctct ggagcctgta cctaaccacg
     1561 aagagctgtt caatcttgcc aaggatgcag taaaggctct caacaaggtt cacgggatcc
     1621 agtacatttt tggcagcatc agcaccaccc tctattcagc cagtgggatc tcggtggact
     1681 gggcttatga cagcggcatc aagtatgcct tcagcttcga gctccgggac actggacaat
     1741 atggcttcct gctgccagcc tcacagattg tccccacagc cgaggagaca tggatggcat
     1801 tacagaccat catgaaacac accctgaatc acccctatta gccacgctac tcaggccgga
     1861 gcagttgggg ttcacccttt tccctgaaaa ccctcctgaa ttaagtatcc cttccccaaa
     1921 ccgtcgttgg acccttagca aatagaagca aatttgagca ggcctcactg cctctagtgt
     1981 tggctgccat ctctttgggc atcccctcat gtctgggaca cccactccca ccccgcccca
     2041 ccccttgctg ccttcgggaa aatatgaggc ccatccaagc tctctttgct tccaatccct
     2101 gggaagggcc aaaatctaca ttgccctagc agtttttgta aaatatttta ataaatccag
     2161 aggggaccag gcttgattta aacaaca
//
