LOCUS       NM_025350               1594 bp    mRNA    linear   ROD 09-FEB-2025
DEFINITION  Mus musculus carboxypeptidase A1, pancreatic (Cpa1), mRNA.
ACCESSION   NM_025350 XM_284174
VERSION     NM_025350.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1594)
  AUTHORS   Ortiz,J.A., Ghazalli,N., Lopez,K., Rawson,J., McCown,E.M., Oh,E.,
            Irimia,J.M., Jou,K., Mares,J., Chen,M.H., Wu,X., Zook,H.N.,
            Quijano,J.C., Erdem,N., Lizarraga,A., Kandeel,F., Fueger,P.T.,
            Thurmond,D.C. and Ku,H.T.
  TITLE     Trefoil Factor 2 Expressed by the Murine Pancreatic Acinar Cells Is
            Required for the Development of Islets and for beta-Cell Function
            During Aging
  JOURNAL   Diabetes 73 (9), 1447-1461 (2024)
   PUBMED   38905124
REFERENCE   2  (bases 1 to 1594)
  AUTHORS   Lu,P., Xu,J., Shen,X., Sun,J., Liu,M., Niu,N., Wang,Q. and Xue,J.
  TITLE     Spatiotemporal role of SETD2-H3K36me3 in murine pancreatic
            organogenesis
  JOURNAL   Cell Rep 43 (2), 113703 (2024)
   PUBMED   38265933
REFERENCE   3  (bases 1 to 1594)
  AUTHORS   Darrigrand,J.F., Salowka,A., Torres-Cano,A., Tapia-Rojo,R., Zhu,T.,
            Garcia-Manyes,S. and Spagnoli,F.M.
  TITLE     Acinar-ductal cell rearrangement drives branching morphogenesis of
            the murine pancreas in an IGF/PI3K-dependent manner
  JOURNAL   Dev Cell 59 (3), 326-338 (2024)
   PUBMED   38237591
REFERENCE   4  (bases 1 to 1594)
  AUTHORS   Cobo,I., Paliwal,S., Bodas,C., Felipe,I., Melia-Aloma,J.,
            Torres,A., Martinez-Villarreal,J., Malumbres,M., Garcia,F.,
            Millan,I., Del Pozo,N., Park,J.C., MacDonald,R.J., Munoz,J.,
            Mendez,R. and Real,F.X.
  TITLE     NFIC regulates ribosomal biology and ER stress in pancreatic acinar
            cells and restrains PDAC initiation
  JOURNAL   Nat Commun 14 (1), 3761 (2023)
   PUBMED   37353485
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 1594)
  AUTHORS   Martinez-Ramirez,A.S., Borders,T.L., Paul,L., Schipma,M., Wang,X.,
            Korobova,F., Wright,C.V. and Sosa-Pineda,B.
  TITLE     Specific Temporal Requirement of Prox1 Activity During Pancreatic
            Acinar Cell Development
  JOURNAL   Gastro Hep Adv 1 (5), 807-823 (2022)
   PUBMED   37829188
REFERENCE   6  (bases 1 to 1594)
  AUTHORS   Bahary,N., Pachter,J.E., Felman,R., Leibel,R.L., Albright,K.,
            Cram,S. and Friedman,J.M.
  TITLE     Molecular mapping of mouse chromosomes 4 and 6: use of a
            flow-sorted Robertsonian chromosome
  JOURNAL   Genomics 13 (3), 761-769 (1992)
   PUBMED   1639403
REFERENCE   7  (bases 1 to 1594)
  AUTHORS   Serikawa,T., Kuramoto,T., Hilbert,P., Mori,M., Yamada,J.,
            Dubay,C.J., Lindpainter,K., Ganten,D., Guenet,J.L., Lathrop,G.M. et
            al.
  TITLE     Rat gene mapping using PCR-analyzed microsatellites
  JOURNAL   Genetics 131 (3), 701-721 (1992)
   PUBMED   1628813
REFERENCE   8  (bases 1 to 1594)
  AUTHORS   Gittes,G.K. and Rutter,W.J.
  TITLE     Onset of cell-specific gene expression in the developing mouse
            pancreas
  JOURNAL   Proc Natl Acad Sci U S A 89 (3), 1128-1132 (1992)
   PUBMED   1371010
REFERENCE   9  (bases 1 to 1594)
  AUTHORS   Siegel,D., Irving,N.G., Friedman,J.M. and Wainwright,B.J.
  TITLE     Localization of the cystic fibrosis transmembrane conductance
            regulator (Cftr) to mouse chromosome 6
  JOURNAL   Cytogenet Cell Genet 61 (3), 184-185 (1992)
   PUBMED   1385049
REFERENCE   10 (bases 1 to 1594)
  AUTHORS   Steinmeyer,K., Klocke,R., Ortland,C., Gronemeier,M., Jockusch,H.,
            Grunder,S. and Jentsch,T.J.
  TITLE     Inactivation of muscle chloride channel by transposon insertion in
            myotonic mice
  JOURNAL   Nature 354 (6351), 304-308 (1991)
   PUBMED   1659665
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BC052661.1 and AC155656.10.
            
            On Jan 23, 2016 this sequence version replaced NM_025350.3.
            
            Summary: This gene encodes carboxypeptidase A, a zinc-dependent
            metalloprotease that cleaves peptide bonds at the C-terminus of
            protein substrates. The encoded preproprotein undergoes proteolytic
            activation to generate a mature, functional enzyme. This gene is
            expressed in pancreas, the encoded protein is a major component of
            digestive enzymes secreted by pancreas and plays an important role
            in the process of digestion. This gene is located in a cluster of
            related carboxypeptidase genes on chromosome 6. [provided by
            RefSeq, Jan 2016].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC052661.1, AK003088.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849377
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-208               BC052661.1         12-219
            209-209             AC155656.10        104190-104190
            210-1594            BC052661.1         221-1605
FEATURES             Location/Qualifiers
     source          1..1594
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="6"
                     /map="6 12.52 cM"
     gene            1..1594
                     /gene="Cpa1"
                     /gene_synonym="0910001L12Rik; Cpa"
                     /note="carboxypeptidase A1, pancreatic"
                     /db_xref="GeneID:109697"
                     /db_xref="MGI:MGI:88478"
     exon            1..311
                     /gene="Cpa1"
                     /gene_synonym="0910001L12Rik; Cpa"
                     /inference="alignment:Splign:2.1.0"
     CDS             247..1506
                     /gene="Cpa1"
                     /gene_synonym="0910001L12Rik; Cpa"
                     /EC_number="3.4.17.1"
                     /codon_start=1
                     /product="carboxypeptidase A1 preproprotein"
                     /protein_id="NP_079626.2"
                     /db_xref="CCDS:CCDS19977.1"
                     /db_xref="GeneID:109697"
                     /db_xref="MGI:MGI:88478"
                     /translation="MKRLLVLSVLLAAVFGNENFVGHQVLRISATDEAQVQKVRELEE
                     LEHLKLDFWRDPARAGLPIDVRVPFPTIQSVKAFLEYHDISYEIMIEDVQSLLDEEKQ
                     QMSAFQARALSTDAFNYATYHTLDEIYEFMDLLVTEHPQLVSKIQIGSTFEGRPINVL
                     KFSTGGTNRPAIWIDTGIHSREWVTQASGVWFAKKITKDYGQEPTLTAILDNMDIFLE
                     IVTNPDGFVYTHKTNRMWRKTRSHTEGSLCVGVDPNRNWDAAFGMPGASSNPCSETYR
                     GKFPNSEVEVKSIVDFVTSHGNIKAFISIHSYSQLLLYPYGYTSEPAPDKEELDQLAK
                     SAVTALTSLHGTKFKYGSIIDTIYQASGSTIDWTYSQGIKYSFTFELRDTGLRGFLLP
                     ASQIIPTAEETWLALLTIMDHTVKHPY"
     exon            312..393
                     /gene="Cpa1"
                     /gene_synonym="0910001L12Rik; Cpa"
                     /inference="alignment:Splign:2.1.0"
     exon            394..627
                     /gene="Cpa1"
                     /gene_synonym="0910001L12Rik; Cpa"
                     /inference="alignment:Splign:2.1.0"
     exon            628..729
                     /gene="Cpa1"
                     /gene_synonym="0910001L12Rik; Cpa"
                     /inference="alignment:Splign:2.1.0"
     exon            730..831
                     /gene="Cpa1"
                     /gene_synonym="0910001L12Rik; Cpa"
                     /inference="alignment:Splign:2.1.0"
     exon            832..942
                     /gene="Cpa1"
                     /gene_synonym="0910001L12Rik; Cpa"
                     /inference="alignment:Splign:2.1.0"
     exon            943..1033
                     /gene="Cpa1"
                     /gene_synonym="0910001L12Rik; Cpa"
                     /inference="alignment:Splign:2.1.0"
     exon            1034..1233
                     /gene="Cpa1"
                     /gene_synonym="0910001L12Rik; Cpa"
                     /inference="alignment:Splign:2.1.0"
     exon            1234..1318
                     /gene="Cpa1"
                     /gene_synonym="0910001L12Rik; Cpa"
                     /inference="alignment:Splign:2.1.0"
     exon            1319..1581
                     /gene="Cpa1"
                     /gene_synonym="0910001L12Rik; Cpa"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1560..1565
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Cpa1"
                     /gene_synonym="0910001L12Rik; Cpa"
                     /note="hexamer: AATAAA"
     polyA_site      1581
                     /gene="Cpa1"
                     /gene_synonym="0910001L12Rik; Cpa"
                     /note="major polyA site"
ORIGIN      
        1 cccagctggg agacctcagg gacagctgac cccatggtca agggtgaaag cctggcttat
       61 ctctccacct gccttgttcc ctgatacttt atcaggaaga gaggaaaggt gccacagttt
      121 ggaactcctg cctctccctc cccatgagac ttgatccaga tgtgagggga actgtcccca
      181 gggaccctcg tcagtgttta aaaaggcctc agacctcagt cttggtctca gcctgaccgt
      241 ctcgccatga agaggctgct ggttctgagt gtgctgctgg cagcagtctt cggcaatgag
      301 aactttgtgg ggcaccaggt tctccgaatc tctgccaccg acgaagccca ggtgcagaaa
      361 gtgagggagc tggaggagtt ggagcacttg aagttggact tctggcggga ccctgcccgg
      421 gctggtctcc ccatcgatgt tcgagtgccc ttccccacca tccagtccgt gaaagcattc
      481 ttggaatatc atgatattag ctatgagatc atgatcgaag atgtgcagtc tctgctcgac
      541 gaggagaagc agcagatgtc cgccttccag gccagggctc tgtccaccga tgctttcaat
      601 tatgccacct accatactct ggatgagatc tatgaattca tggacctgct ggttacagag
      661 cacccacagc ttgtaagcaa gatccagatc ggcagcacct ttgaaggtcg tcccatcaat
      721 gtgctgaagt tcagcactgg agggaccaat cgcccagcaa tctggattga cactggcatc
      781 cattccaggg agtgggtcac tcaggccagt ggggtctggt ttgcaaaaaa gatcacgaag
      841 gactatggcc aggaacccac cctcacggcc attcttgaca acatggacat cttcctggag
      901 attgtaacta accctgatgg ttttgtctac acccacaaaa cgaatcgcat gtggcgcaaa
      961 actcgatcac acaccgaggg ctccctctgc gtcggggtgg accccaacag gaactgggat
     1021 gctgcctttg ggatgcccgg agctagtagc aacccctgct cagaaactta ccgtggcaag
     1081 tttcccaact ctgaagtgga ggtcaagtcc atcgtggact ttgtgacgag ccacgggaac
     1141 atcaaggcct tcatctccat ccacagctac tcccagctcc ttctctaccc ctatggctac
     1201 acatcagaac cagctcctga caaggaggag ctggatcagc tagcaaaatc tgctgtgaca
     1261 gccttgacat ctctacacgg gaccaagttc aagtatggca gtatcattga tacgatctat
     1321 caagccagtg ggagcactat tgactggacc tacagccagg gcatcaagta ttccttcact
     1381 tttgagctga gggacactgg gctcagaggt ttcctgctgc ctgcctccca gatcatcccc
     1441 acagcggagg agacatggct ggcccttttg accatcatgg accacactgt caaacacccc
     1501 tactgaagaa gcttttattc ccttcccctt tcttctcctt agccccattt aggcaaccca
     1561 ataaagtctg attgtaaact aaaaaaaaaa aaaa
//
