LOCUS       NM_011931              12474 bp    mRNA    linear   ROD 08-FEB-2024
DEFINITION  Mus musculus COP1, E3 ubiquitin ligase (Cop1), transcript variant
            1, mRNA.
ACCESSION   NM_011931
VERSION     NM_011931.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 12474)
  AUTHORS   Lynes,M.D., Huang,Q., Cora,C., Su,S.C., Yi,P. and Tseng,Y.H.
  TITLE     A CRISPR Screen Identifies the E3 Ubiquitin Ligase Rfwd2 as a
            Negative Regulator of Glucose Uptake in Brown Adipocytes
  JOURNAL   Genes (Basel) 14 (10), 1865 (2023)
   PUBMED   37895214
  REMARK    GeneRIF: A CRISPR Screen Identifies the E3 Ubiquitin Ligase Rfwd2
            as a Negative Regulator of Glucose Uptake in Brown Adipocytes.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12474)
  AUTHORS   He,C., Wang,T., Han,Y., Zuo,C. and Wang,G.
  TITLE     E3 ubiquitin ligase COP1 confers neuroprotection in cerebral
            ischemia/reperfusion injury via regulation of transcription factor
            C/EBPbeta in microglia
  JOURNAL   Int J Biol Macromol 222 (Pt B), 1789-1800 (2022)
   PUBMED   36191790
  REMARK    GeneRIF: E3 ubiquitin ligase COP1 confers neuroprotection in
            cerebral ischemia/reperfusion injury via regulation of
            transcription factor C/EBPbeta in microglia.
REFERENCE   3  (bases 1 to 12474)
  AUTHORS   Wang,X., Tokheim,C., Gu,S.S., Wang,B., Tang,Q., Li,Y., Traugh,N.,
            Zeng,Z., Zhang,Y., Li,Z., Zhang,B., Fu,J., Xiao,T., Li,W.,
            Meyer,C.A., Chu,J., Jiang,P., Cejas,P., Lim,K., Long,H., Brown,M.
            and Liu,X.S.
  TITLE     In vivo CRISPR screens identify the E3 ligase Cop1 as a modulator
            of macrophage infiltration and cancer immunotherapy target
  JOURNAL   Cell 184 (21), 5357-5374 (2021)
   PUBMED   34582788
  REMARK    GeneRIF: In vivo CRISPR screens identify the E3 ligase Cop1 as a
            modulator of macrophage infiltration and cancer immunotherapy
            target.
REFERENCE   4  (bases 1 to 12474)
  AUTHORS   Lin,H., Yan,Y., Luo,Y., So,W.Y., Wei,X., Zhang,X., Yang,X.,
            Zhang,J., Su,Y., Yang,X., Zhang,B., Zhang,K., Jiang,N.,
            Chow,B.K.C., Han,W., Wang,F. and Rao,F.
  TITLE     IP6-assisted CSN-COP1 competition regulates a CRL4-ETV5 proteolytic
            checkpoint to safeguard glucose-induced insulin secretion
  JOURNAL   Nat Commun 12 (1), 2461 (2021)
   PUBMED   33911083
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 12474)
  AUTHORS   Ndoja,A., Reja,R., Lee,S.H., Webster,J.D., Ngu,H., Rose,C.M.,
            Kirkpatrick,D.S., Modrusan,Z., Chen,Y.J., Dugger,D.L., Gandham,V.,
            Xie,L., Newton,K. and Dixit,V.M.
  TITLE     Ubiquitin Ligase COP1 Suppresses Neuroinflammation by Degrading
            c/EBPbeta in Microglia
  JOURNAL   Cell 182 (5), 1156-1169 (2020)
   PUBMED   32795415
REFERENCE   6  (bases 1 to 12474)
  AUTHORS   Dentin,R., Liu,Y., Koo,S.H., Hedrick,S., Vargas,T., Heredia,J.,
            Yates,J. 3rd and Montminy,M.
  TITLE     Insulin modulates gluconeogenesis by inhibition of the coactivator
            TORC2
  JOURNAL   Nature 449 (7160), 366-369 (2007)
   PUBMED   17805301
REFERENCE   7  (bases 1 to 12474)
  AUTHORS   Qi,L., Heredia,J.E., Altarejos,J.Y., Screaton,R., Goebel,N.,
            Niessen,S., Macleod,I.X., Liew,C.W., Kulkarni,R.N., Bain,J.,
            Newgard,C., Nelson,M., Evans,R.M., Yates,J. and Montminy,M.
  TITLE     TRB3 links the E3 ubiquitin ligase COP1 to lipid metabolism
  JOURNAL   Science 312 (5781), 1763-1766 (2006)
   PUBMED   16794074
  REMARK    GeneRIF: description of a pathway in which Tribbles 3 (TRB3)
            stimulates lipolysis by triggering the degradation of
            acetyl-coenzyme A carboxylase (ACC) in adipose tissue; TRB3
            promoted ACC ubiquitination through an association with the E3
            ubiquitin ligase COP1
REFERENCE   8  (bases 1 to 12474)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
REFERENCE   9  (bases 1 to 12474)
  AUTHORS   Yi,C., Wang,H., Wei,N. and Deng,X.W.
  TITLE     An initial biochemical and cell biological characterization of the
            mammalian homologue of a central plant developmental switch, COP1
  JOURNAL   BMC Cell Biol 3, 30 (2002)
   PUBMED   12466024
REFERENCE   10 (bases 1 to 12474)
  AUTHORS   Wang,H., Kang,D., Deng,X.W. and Wei,N.
  TITLE     Evidence for functional conservation of a mammalian homologue of
            the light-responsive plant protein COP1
  JOURNAL   Curr Biol 9 (13), 711-714 (1999)
   PUBMED   10395541
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CJ070274.1, BC082804.1 and AC084287.9.
            
            [WARNING] On Mar 30, 2024 this sequence was replaced by
            NM_011931.5.
            
            On Feb 16, 2018 this sequence version replaced NM_011931.3.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC082804.1, AF151110.2 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-126               CJ070274.1         4-129
            127-5120            BC082804.1         1-4994
            5121-12474          AC084287.9         186653-194006
FEATURES             Location/Qualifiers
     source          1..12474
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="1"
                     /map="1 69.03 cM"
     gene            1..12474
                     /gene="Cop1"
                     /gene_synonym="Rfwd2"
                     /note="COP1, E3 ubiquitin ligase"
                     /db_xref="GeneID:26374"
                     /db_xref="MGI:MGI:1347046"
     exon            1..651
                     /gene="Cop1"
                     /gene_synonym="Rfwd2"
                     /inference="alignment:Splign:2.1.0"
     CDS             239..2440
                     /gene="Cop1"
                     /gene_synonym="Rfwd2"
                     /EC_number="2.3.2.27"
                     /note="isoform 1 is encoded by transcript variant 1; E3
                     ubiquitin-protein ligase RFWD2; mCOP1; RING finger and WD
                     repeat domain protein 2; constitutive photomorphogenesis
                     protein 1 homolog; RING-type E3 ubiquitin transferase
                     RFWD2; constitutive photomorphogenic protein 1; ring
                     finger and WD repeat domain 2; E3 ubiquitin-protein ligase
                     COP1"
                     /codon_start=1
                     /product="E3 ubiquitin-protein ligase COP1 isoform 1"
                     /protein_id="NP_036061.1"
                     /db_xref="CCDS:CCDS35745.1"
                     /db_xref="GeneID:26374"
                     /db_xref="MGI:MGI:1347046"
                     /translation="MSGSRQAGSGSAGTSPGSSAASSVTSASSSLSSSPSPPSVAASA
                     ATLVSGGVAPAAGSGGLGGPGRPVLVAAAVSGSASAGGAVSAGQSRLSCAARPSAGVG
                     GSSSSLGSSSRKRPLLVPLCNGLLNSYEDKSNDFVCPICFDMIEEAYMTKCGHSFCYK
                     CIHQSLEDNNRCPKCNYVVDNIDHLYPNFLVNELILKQKQRFEEKRFKLDHSVSSTNG
                     HRWQIFQDLLGTDQDNLDLANVNLMLELLVQKKKQLEAESHAAQLQILMEFLKVARRN
                     KREQLEQIQKELSVLEEDIKRVEEMSGLYSPVSEDSTVPQFEAPSPSHSSIIDSTEYS
                     QPPGFSGTSQTKKQPWYNSTLASRRKRLTAHFEDLEQCYFSTRMSRISDDSRTASQLD
                     EFQECLSKFTRYNSVRPLATLSYASDLYNGSSIVSSIEFDRDCDYFAIAGVTKKIKVY
                     EYGTVIQDAVDIHYPENEMTCNSKISCISWSSYHKNLLASSDYEGTVILWDGFTGQRS
                     KVYQEHEKRCWSVDFNLMDPKLLASGSDDAKVKLWSTNLDNSVASIEAKANVCCVKFS
                     PSSRYHLAFGCADHCVHYYDLRNTKQPIMVFKGHRKAVSYAKFVSGEEIVSASTDSQL
                     KLWNVGKPYCLRSFKGHINEKNFVGLASNGDYIACGSENNSLYLYYKGLSKTLLTFKF
                     DTVKSVLDKDRKEDDTNEFVSAVCWRALSDGESNVLIAANSQGTIKVLELV"
     exon            652..711
                     /gene="Cop1"
                     /gene_synonym="Rfwd2"
                     /inference="alignment:Splign:2.1.0"
     exon            712..809
                     /gene="Cop1"
                     /gene_synonym="Rfwd2"
                     /inference="alignment:Splign:2.1.0"
     exon            810..886
                     /gene="Cop1"
                     /gene_synonym="Rfwd2"
                     /inference="alignment:Splign:2.1.0"
     exon            887..1006
                     /gene="Cop1"
                     /gene_synonym="Rfwd2"
                     /inference="alignment:Splign:2.1.0"
     exon            1007..1075
                     /gene="Cop1"
                     /gene_synonym="Rfwd2"
                     /inference="alignment:Splign:2.1.0"
     exon            1076..1135
                     /gene="Cop1"
                     /gene_synonym="Rfwd2"
                     /inference="alignment:Splign:2.1.0"
     exon            1136..1212
                     /gene="Cop1"
                     /gene_synonym="Rfwd2"
                     /inference="alignment:Splign:2.1.0"
     exon            1213..1270
                     /gene="Cop1"
                     /gene_synonym="Rfwd2"
                     /inference="alignment:Splign:2.1.0"
     exon            1271..1385
                     /gene="Cop1"
                     /gene_synonym="Rfwd2"
                     /inference="alignment:Splign:2.1.0"
     exon            1386..1521
                     /gene="Cop1"
                     /gene_synonym="Rfwd2"
                     /inference="alignment:Splign:2.1.0"
     exon            1522..1665
                     /gene="Cop1"
                     /gene_synonym="Rfwd2"
                     /inference="alignment:Splign:2.1.0"
     exon            1666..1774
                     /gene="Cop1"
                     /gene_synonym="Rfwd2"
                     /inference="alignment:Splign:2.1.0"
     exon            1775..1856
                     /gene="Cop1"
                     /gene_synonym="Rfwd2"
                     /inference="alignment:Splign:2.1.0"
     exon            1857..1973
                     /gene="Cop1"
                     /gene_synonym="Rfwd2"
                     /inference="alignment:Splign:2.1.0"
     exon            1974..2091
                     /gene="Cop1"
                     /gene_synonym="Rfwd2"
                     /inference="alignment:Splign:2.1.0"
     exon            2092..2216
                     /gene="Cop1"
                     /gene_synonym="Rfwd2"
                     /inference="alignment:Splign:2.1.0"
     exon            2217..2377
                     /gene="Cop1"
                     /gene_synonym="Rfwd2"
                     /inference="alignment:Splign:2.1.0"
     exon            2378..2422
                     /gene="Cop1"
                     /gene_synonym="Rfwd2"
                     /inference="alignment:Splign:2.1.0"
     exon            2423..12474
                     /gene="Cop1"
                     /gene_synonym="Rfwd2"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gttggctgcc ccggcggcct cctcggcagt catggctcct cggatgtgat agtttccccc
       61 gccccctcgc gtaggacgcg gcggcggggc ggcggtggga agcctaactt tattgttccc
      121 ccggcccgtc tcccggcgcg tcctcaggat gctcgggagc tgagcgccgg gggcggcgga
      181 gggtcgcggt ctgtccgggc cctcgcctcg gagcgcaggg ccaagggagg gagtcacgat
      241 gtcaggtagc cgccaggccg gctcgggttc cgctgggacg agccccggct cctcggccgc
      301 ctcctcggtg acttccgcct cctcgtcctt atcctcgtct ccgtcgccgc cgtccgtggc
      361 ggcctcggcg gcgacgctgg tgtccggcgg cgtggctccg gccgcgggct ccggcggcct
      421 cggcggcccg gggcggcctg tgctggtggc ggccgcggtg tctggcagcg cgagcgccgg
      481 cggggcggtg tccgcgggcc agtcccggct cagctgcgcg gccaggccca gcgccggcgt
      541 tggagggagc agctccagcc tcggcagtag cagtaggaag cgacctctgc tcgtcccgct
      601 ttgtaacggg ctcctcaact cctacgagga caaaagcaac gacttcgtct gtcccatctg
      661 ctttgatatg attgaggagg catacatgac aaaatgtggc cacagctttt gctacaagtg
      721 tattcatcag agtttggagg acaataatag atgtcccaag tgtaattatg ttgtggacaa
      781 tattgaccat ctctatccta atttcttggt gaatgaactc attctcaaac agaagcaaag
      841 atttgaggaa aagaggttca aattggacca ctcagtgagt agcaccaatg ggcataggtg
      901 gcaaatattt caagatctac taggaactga tcaagataac cttgatttgg ccaatgtcaa
      961 cctcatgttg gaattattag tgcagaagaa gaaacaactg gaagcagaat cacatgcagc
     1021 tcagctacag atccttatgg aattcctcaa ggttgcaagg agaaataaga gagagcaatt
     1081 ggagcagatc cagaaggaac taagtgtttt ggaagaggat attaaaagag tggaagaaat
     1141 gagtggcctc tactctcctg tcagtgaaga tagcacagtg cctcaatttg aagctccttc
     1201 tccatcacac agtagcatta ttgattctac agaatacagc caacctccag gtttcagtgg
     1261 gacctctcag acaaagaaac agccttggta taacagcaca ttagcatcaa gacgaaagcg
     1321 actcactgct cattttgaag acttagagca gtgttatttt tctacaagga tgtctcgtat
     1381 ctcagatgac agtagaactg caagccagtt agatgaattt caagaatgct tgtccaagtt
     1441 tactcgatac aactcagtaa gaccgttggc cacattgtct tatgctagtg atctctataa
     1501 tggttccagc atagtttcta gtattgagtt tgaccgggat tgtgactatt ttgcaattgc
     1561 tggagttaca aagaagatta aagtctatga gtatggcaca gtcatccagg atgcagtgga
     1621 tattcattac cctgagaatg aaatgacctg caattccaaa atcagctgta tcagttggag
     1681 tagttaccat aagaacctgc tagccagcag tgattatgaa ggcactgtta tactatggga
     1741 tggattcaca ggacagaggt caaaggtcta tcaggagcat gaaaaaaggt gttggagtgt
     1801 tgactttaat ctgatggatc ctaaacttct ggcttcaggt tctgatgatg caaaagtgaa
     1861 gctgtggtct accaatttag acaattctgt ggcgagcatt gaggcaaagg ctaacgtgtg
     1921 ctgtgtgaag ttcagcccct cctccaggta ccatctggct ttcggctgtg cagatcactg
     1981 tgtccactac tacgaccttc gtaacactaa acagccaata atggtattca agggacaccg
     2041 aaaagcagtg tcttatgcca agtttgtaag tggtgaagaa attgtctcag cctcaacaga
     2101 cagccaacta aagctgtgga atgtgggaaa accatactgt ctacgttcct tcaagggtca
     2161 tattaatgaa aaaaactttg taggccttgc ttccaatgga gattatatag cgtgtggaag
     2221 tgagaacaac tccctctacc tgtattataa aggactttct aaaactttgc taacttttaa
     2281 gtttgatacc gttaagagtg tattagacaa agatcggaaa gaagatgaca caaatgaatt
     2341 tgtcagtgct gtgtgttggc gggcactatc agacggggag tccaatgtgc tgattgctgc
     2401 taacagccag ggtacaatta aggtgctaga attggtataa agagtttcct gaagtcaaac
     2461 tgtgtttgat cctcccaaaa gccatctgca gctgatgatg agaagaaaaa gaaatggcat
     2521 gtaatgtctc ccccaacatc atttggattt gttttaagta ccttttccct cctttatgcc
     2581 tttgggacac tgggaatatc cagagaattc gccaccatca gtgtaactat gaactttgct
     2641 gttggcagtg tggctaattt ttgtgatagg gaaacaaatt cttttaaata aaaataataa
     2701 acaacaacaa caaaaagttt attgagccac agctgagcta ggaacacctt tttgtcagat
     2761 ggggtgagag tcttttgaag aagttgcact tatgggttat acagaatgtt ctggctgggg
     2821 ctggagagag gctcagagaa taagagcact ggtcgctcct acagaggatc tgggttcagt
     2881 ccccagcacc tacgtagcag ctcactgctg cttatgattc cagtaggaga tctgacgtcc
     2941 tcttctgggc tccttaagca ccaggcatgc atgtggtgca cattcaaaga tccagataca
     3001 acgctcacac ataaatgaaa ataaatctta ttttaaaagg aagaaaaatg tactggtgag
     3061 taattttatc gttgtgtttc tcaacccttt tggaatatca acataaacaa aagataaaat
     3121 gtgtagattc cttccaggga ccttaatgtt tacaatttaa aaaaaataat aagaattcct
     3181 ttttctatac cctgattaga tgatcaccat cagaccttat ctggtcagag atctaagagt
     3241 ttgacttgag atcaagtaaa caggaaagga ctgtccttac aactctggtt ctctttgcac
     3301 caagaatccc tcagttatac tggccaagac tgatcactga ctgagaagag gtcaaccaac
     3361 cttttctata aaagaacata taggaaatac ttcaggcttt gctggccata tggtctctta
     3421 taactgttag agaactgtta gctctgccag ggtatcaggg atgtagctac tatcgttagt
     3481 agacaaatgg gcatggctag aaaataggtg gaatttcaaa ctctatctgc ggctcatctg
     3541 caatgccctg atttaaactt gtatgctctg gtacctaccc caagtttcca aaccgtgtga
     3601 cctcttaaat tgtctagtta atttataaat catttttaca ttgcagggca ttgggtcacg
     3661 caaaattaat gtggataata catttcagca aatggttcaa gtgaaattct ccacacattt
     3721 cataaaacat tttcacaaag gattgttgga tacagatagc acaatccact acccagtgga
     3781 tccctcagct gaaggttact gatacctaag taagcttaca tttttttgaa cttaaattat
     3841 tgaagccagt tgtcttttga ggttttaaac tctttaaact accagaaagt cacataacac
     3901 acataactat attaacagca atcccaacaa aactaaagca atggtacatg tccaacataa
     3961 acttctgctg caaagcctgc atagtcacat tccatgaggc tggttagaaa agaggaaact
     4021 taggaaagag gagaaataat tatacaatag tttgtgtaag gttttatcat ccaacttgag
     4081 tgaaatttat tagttttcat agcttcaaaa gaatttcatc tttcataact tcttacattt
     4141 aaaacttgct aaacttaatg gtctcaagtc tgagagaaaa ggctcagcag tcaagagtcc
     4201 tgctgctctt gtagagaacc caagtttgct tcctaggacc cacagatgag ctcacaacta
     4261 cttagaaccc caattccaaa gaatctgaca ttctatttta acatccaagg cccaaggcat
     4321 ggatgtggta cacagacata tgtgcaggag aaacacctat acatataaat aaatccttaa
     4381 aaaaatcaga agttaccatc tgtcaccagt gaatgttaaa atcagtaaat gtccatttgt
     4441 gaagatgtag gaagtatact ccaacagaga tgctggtgtt agggatgagt gccctgaaca
     4501 tcttcaagat aggaagcagt tagtgccacc ttggatgcct ttaaatagaa gtgatgtttt
     4561 tgctcattaa tactgttttt acatgcctac acttttaatc tcaagctctc tatattatat
     4621 gagttgatca tgttagtttc catgtttaga aaatatagaa ctgtttggtt atcaaaggga
     4681 tatggctttt tagaaataaa ttattttaag atagtgcttt tttttttttt ttaaagaaag
     4741 tttaccatgt agcacaaacc aaactcagtt ttactctgta gtcacaagta gccctgagct
     4801 tctgatcatc ctgtcgcccc accccggggc tgctgggata taatcatgtg tcccccctcc
     4861 cagttttaag tggtgctaaa gtgctaggga agaaaaccta gggctctgtg catgctaggc
     4921 aagacactta tcaactgaac tacatctgta accccttagt tagatttttt ttttcctttt
     4981 tcttgaaaat catagctttc taaagttgac ttagtctctg caggacattg agatcaaatt
     5041 aataatgcta ttccttgtaa aaatataaca cttaaattat gttttacctt aatctttatt
     5101 tcaggggttt tgtagggttt cttacacaac ttgtctttat tcccatttgt caactaatgt
     5161 gaataaaaaa ttgaagtttg tattactttg tagaattatt gtaaattgac ctgatattaa
     5221 aaacaaatta agaaagcaaa gctagatatg gtggcacact tctgtcatct tggtgcttgg
     5281 gaagtaggag gcagggagtc atgaattcag gctcattctc aacagcagag tgcagtctag
     5341 accacccaca ctacctgaaa tgaaatatgg aacccagcta cagcttttag aaagaagaaa
     5401 gaacacagga tgagtttgaa cagaggagcc caatacaatg gcacttttag cacatgatta
     5461 agaatttgga tttttataaa gcagaatggt gggatctggt cacgggaaaa tagttggagg
     5521 ttaataggag cttgggtcaa agataacgtt agccacccca aatcctgcat catctattgg
     5581 cattcaccac agtctttact aagagaactt accctaggga gtttattttt tcaccgattg
     5641 tattatcctc agcaagctta agttacttga aaacatcatg gccacagtgc ttacgaacct
     5701 gtagctaagc aaatctagaa gacatctagc cctagttcta aacataaggg taatgggtca
     5761 ttgcccttaa tgcaattatg caaatacagc tagtgttggc tacttaattg agcccattgt
     5821 aatactgctg tgctcttcct cacagagcag tcagtttgac atcttatatg atgcagcttc
     5881 tgtttaatgg gttaggaaac tgtggagccc tcagttctgg aaatatttgt tgaacagctg
     5941 cttttcgtga gaattgtgtc ctacattgta agaaaattat aattatgcta gtgtagctta
     6001 ttcactaaga gagagagaga gacagggacc caggcaaaaa tatggaagac atgagttttg
     6061 tgtgttcact gggaaaacat acaaccttac atgatgggag atgaagggtt ggggtagagg
     6121 tagttgtcag gaagtttaat cttacaggag ataaaaatct cagctgaatc ttaaactagt
     6181 taaaaagaaa aagtcaaatg aatttaaggg caacagattt taagaaaaat gtgtgtttct
     6241 cttgaccaaa actgaaatat gtcagcaaca ggataaacat cggaatgcag tcaaaagtat
     6301 gggacaaatc gtaaagccat actactgtaa gtaagataca gaattgcagt ttgtggctat
     6361 acctcaccct caagatacct tttgggacat gcctccactt tgagtatggt ctgtatatag
     6421 tgattttttc cccaaaggta tattttggtt aagaagataa aagttagtta ctacaatgga
     6481 gaagcttaca gaactacttt aattaatcta accatggcca acatcaaaga tgatgtcata
     6541 ctgatagtat atactgttga taagatataa aagaaatggt gtttttactc tgtagtcatc
     6601 atgtaaatgg gaaagacatc catcatataa gccctccttg agttataacc tactaaataa
     6661 ccgactagcc tttctaaact gtcagaacca tcaaaaacaa agccagagaa attgtcacag
     6721 gcacaaggag aatagagact gagcaaatgg tctgcactgt ccagggtgat attctgaaac
     6781 tgaaagggac agtgcataaa atagagaaaa gtcaagcatg gctttctctg atatcagtgg
     6841 ttctgcaccg gtgtgctgtt tataacaaat tgtcatcata atatgaagtg taaacaggct
     6901 acattaagaa tgtacaagga atacaagggt tttaatattg ccttattaaa cttttttgga
     6961 agtttatttt agtcaagttt atagttttag atcaggaaat tggcttattg gataaaacag
     7021 ttgccttata aagtatgaag accaaagttt caatcttcag agactgccaa tagctgagtg
     7081 ggtaaggcat gtagcctgtg actcaaaatt gataaacaga catggggatt cccagggaaa
     7141 cctggctaaa tattggcacg ctctggtgtt ggccaagatc ctactccata gaaaaatgtg
     7201 aagaacagtc aaggaaggtg cacagaaccc tctgacttac aaagacccat acacacacct
     7261 gcatacctgt gcacgcatcc acaagcattt atacctatac acatgcatac gaactcatac
     7321 cacctccaaa acggaaagga atttcagtat tttttataag tatgaagata caaagcagaa
     7381 tgcaacagga caatgggaat taaagctaac tacataaatg atttacacat gaaatcagag
     7441 taactgtcca gccgaggctt aaacagcaca agtcatggag cttaaagaaa ctgacattgt
     7501 agtgtttatc attaataaag tgtatttttg caaaagatgg aaaatatctt tgtttacctg
     7561 caaattctac ttcctgagag tgaaacttgc aacaacaaaa agcaaagcaa catcttagca
     7621 agattgtaat atataaagtt catagtcatc cttggagttt aaacactcat ttatatatga
     7681 gtgaaaatat tctacctgca ctcattaaat cctcaataag agaaccaaga ggtccttcca
     7741 tttgtgtgtc acttcttgag ttatttgtcc tttgtcagtt ttaagggcaa acttcatatt
     7801 gcctcagatc tgaaatcact ggagttggag taaggaggaa ggtcaatgga atcaaaggtt
     7861 gggctcccaa atttaccatg atactcacag gatttctccc tgtgaacaga ctttttctct
     7921 aattgtcaat gctactctat gaccacaggt ttaggagggt tgatttggac acaggaaact
     7981 tcaatatata ttgttattaa aatttacgtg tgtgggtgat tgcatgtatg tgcaacacgt
     8041 gtgcagcacg tggaggccaa aagagggtgt cagatcctca aactagagtg atgagtttag
     8101 agcctccatg aggatgctgg gaattgaatc caagtcctct agaagagcaa ccagtgctct
     8161 caacagctga gcaattgttc cagccttaag acttttgaaa gaagccaaga aaaagcaatt
     8221 tcatatatgt aatattagca cttggtttta tacagcataa agaacatttt atcccaaaat
     8281 cttaagaatg aggtaagatt gaattgagtt tcttatagca ttctgaccac cacttctact
     8341 caaagccatt atgtcccatc agtctagatg gttgatgtgt tcccaattgt gtgtaaactt
     8401 atgcccatct ggacaatggg ctgccaagtg atagtgatca tatttctgtc tatctctaat
     8461 tctccttagg gtggctctca gctggaagtc tgtaattggc atggcagttg cttacaaagg
     8521 aagatacacc cattgctgct tgctcttggt gggttccttc aacaagttgt agtgccaact
     8581 atatggtaga ccatcctagc caggagtaag caagctgttt ctgtaaaggg ccaactattt
     8641 taggctttgt gggctacaaa tcctccatcg gagccactca gtgttaccgt tgcagcatgc
     8701 aaaaatatcc ctgaaccaaa tataagcaaa cgaatatggt tgtgttccac tacaatcaca
     8761 tatttgagtt catttcactg taatggttca cagatttgta tttttatatt tattaggcat
     8821 taaaatatag aaaacctatg atccgtacaa aaagctacaa actggatttg gtttgcatgc
     8881 tctagcttgc tgacctctag gtgctgacca caaagcagtg taggaaaaag tagtcttcat
     8941 ggaacttcta tttatttgtg gggacggaaa ttctttctgt aaaataatac ctctccccat
     9001 aatggagcat ctctagcata agagcctgga agaccatctt ggctaacacc atccaattca
     9061 aagctacctc gatgcttagt tttcacagaa gtgcacacat gcagtgttgc tatgcattgg
     9121 ttatgtgtct tccagagaac caatggacac ttcctgctga agcttcgtag gatgtaaggt
     9181 cttttgttag gtgggtgtac ttggaggtca atactcacta acaggcttca gagtttaaga
     9241 gcagagatga tactaaagga tcagacagaa agtctgcagt ctgttgggtg tatggaagca
     9301 gggtatattt ttcaaagctg acttcctacc ccttcccact tttgttctct tttactttat
     9361 tattgagatt ctctctctct gagtataaaa tgtttcttga gctcttggca cttaacaaag
     9421 cctgggaacc taatatttcc ttgatgtgac tgaatctcac aaaacatttg tacatggttt
     9481 tatgccattg tttaatcaag acctcttact ttcccctaaa ttaatggcca tcagattagc
     9541 ctgcttttcc agatattgtt catagggaga gtctagactg gtaaatctgt aacctttttt
     9601 ttttcccagt caacacttgt gcttcccaat tctgcatggc caatcccagg agaggcctcc
     9661 gggctctgtg gctccctgga aaaaaggcag ccttctgttc ttagcacagg ctttgccact
     9721 tctgggctgt ggaactgtag cgcttttctg tacattcagt gattattttt gtttgtttgt
     9781 ttgttttgtt ttttttctgg gaaggctgct ggtagtcttg aaaattaaaa gcactccatg
     9841 aacactgggc agcattagaa tttgtgcatg aaaatttcaa tcacttcaat ttgttcctgg
     9901 gaagattaaa gtataaaact gttttttaaa agtagagagt taaaagttag agcagctatt
     9961 ccagaagtct gcaccagtct atgaagtgac ttttattcat tgtgtcttag ttaggctttc
    10021 tattgttata taaaacacta tgacaaaaaa atatcttaga gaacaaagga tttattccac
    10081 cttacagctg tggtccatca tatagaaaag tcagaacacg agcgcaaggc aggaacccgg
    10141 agtcaggagc tgatgcagag gccacggaga agtgcaactt actagctttt ttccccatgg
    10201 cttgctcagt ctgtttatag aacccagggc catcagccca ggagtaacac tacctgtaat
    10261 gagctgggcc tttccacatc atcagcccag aagatgtacc acaggccaat ctggggggtt
    10321 ggggggattt ttatcagttg ttctgccttg cgtcaagttg acataaaact agggagcctt
    10381 gtgttctcaa catgatatga gcctatatcg atatgaacta acctcccaaa gagtgtcttc
    10441 ataactacaa tgaaaagcta ctttacgaaa atgccatttc tcacatactt tcctcggtga
    10501 cggtgggctg aaaagcagca gcacctctat tgattacagg tagcaagaca agtggaaaag
    10561 aaagaagagt tcagttgtca gggaaactct ggaagctaaa gctttgatcc ttaggaaata
    10621 ttcttacctt tctcttcctg tgcccttcta atggtctgaa atcattgcaa gagaaaaaga
    10681 gcgtgtcctc ttcccacctc ttcttggtat gttagacagt ctagtggctt caagtggcca
    10741 tttaactatg cagcagtgta ttcattatct gggaatctca agggaacctt gacatcaatt
    10801 ttatgtctct ccatagctga tcagtggatc attaacttca tgtctctata gatgaaggat
    10861 gagaaaacag ggtcaggatt ttgtgtagaa acctaaccct tgtgacttca gtctcattgt
    10921 atctcagtag gtacaatggt caaacaacca acctcaaact ttctccaaag ccactggtga
    10981 attgatgtag aaattttcat gtggtaaata atgagtagtt ttccagactc tttttaactg
    11041 ctaatagttc ttgagagcca ctggttccta ggatagtttt gaagaatagt tctaattgtt
    11101 aggcaatttt ttgttttgtt ttgttttcct tagattaaac tgaaatctgc ctctcttgca
    11161 tcactcttct tccttcccac taagcttaga gtcatgattt taggagaaag tgctcccata
    11221 ggtatgtgtg ttgaacattt tgtcctcaga tggtggcacc atgtggggaa actagatatt
    11281 ttggaaagtg gagcctagct gaagaaagtg ggttactagg gtttgccttt gaaggttatg
    11341 gctagttatc ccattactca gtttcctgtc tgctaagaag tgaactgcct cctccacata
    11401 ctcttactgc agagatagtt tgccttatca gaggcctgga gccaagaact atactgaaac
    11461 tgtgaaccaa aacaactttt tctccttgta gtttttctta agcattttgt tatagcaatg
    11521 caaacataac taatacattt gccttggctt catcctaggc attggagtgg gaaattgtag
    11581 ataatagcaa gtaaatcgtg atcccttcat gaagctttag gcagacaatt tgcatataat
    11641 aaagtcctat aaagtaaaac attatggtgt ttataaatga aaactgccaa aataatgata
    11701 catagttaaa agattgttta aaaggcatgt ctactgagtt attaagtggg ttgtataaag
    11761 gatttgggat ttcagttgat taaaaaaaaa gttgccgggc agtggtggcg cacaccttta
    11821 atcccagcgc ttgggagaca gaggcaggtg gatttctgag tttgaggcca gcctggtcta
    11881 cagagttgag ttccaggaca accagggcta tacagagaaa ccctatctca aaaaaaaaaa
    11941 aacaaaacaa gacaaaagga ggggggttaa ctgctatttt ctacccacta atcaaacagg
    12001 taccaagaag gcagttacac atgttgatcc tgccaaaaga ctagaaaatg tcttatgatt
    12061 gttgacaagt tacttaactg atgataactt cagatgggta aaacagcaaa agaaagtctt
    12121 cagaacctat cgtgttgtta caacactaaa aggaactcca gtggctatgg acatctgtat
    12181 ccttggagat aactggtcaa ccaagtgtcc aaaatgattt cacagtcaac tgtctgtctg
    12241 aagtctggac ctacatggat catccctcca gatccctttg agagctcaga ctctcctcca
    12301 gattcattca ttcacagaga agaaatcagg aggaatggct atttccctac tgtaataggt
    12361 aatataaaga gtggagagcc tactttccct ttcggaaatt gacttcttcc tccacttcca
    12421 ctttaccctt cttctgtttt gtttttcatc tttttaataa atgtattgaa agtc
//
