LOCUS       NM_001167923            9297 bp    mRNA    linear   ROD 10-JUN-2024
DEFINITION  Mus musculus collagen, type VI, alpha 5 (Col6a5), mRNA.
ACCESSION   NM_001167923 XM_909153 XR_035671
VERSION     NM_001167923.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 9297)
  AUTHORS   Rasheed,A.A.B., Birling,M.C., Lauria,G., Gaveriaux-Ruff,C. and
            Herault,Y.
  TITLE     The COL6A5-p.Glu2272* mutation induces chronic itch in mice
  JOURNAL   Mamm Genome 35 (2), 122-134 (2024)
   PUBMED   38523187
  REMARK    GeneRIF: The COL6A5-p.Glu2272* mutation induces chronic itch in
            mice.
REFERENCE   2  (bases 1 to 9297)
  AUTHORS   Naba,A., Clauser,K.R., Mani,D.R., Carr,S.A. and Hynes,R.O.
  TITLE     Quantitative proteomic profiling of the extracellular matrix of
            pancreatic islets during the angiogenic switch and insulinoma
            progression
  JOURNAL   Sci Rep 7, 40495 (2017)
   PUBMED   28071719
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 9297)
  AUTHORS   Besschetnova,T.Y., Ichimura,T., Katebi,N., St Croix,B.,
            Bonventre,J.V. and Olsen,B.R.
  TITLE     Regulatory mechanisms of anthrax toxin receptor 1-dependent
            vascular and connective tissue homeostasis
  JOURNAL   Matrix Biol 42, 56-73 (2015)
   PUBMED   25572963
REFERENCE   4  (bases 1 to 9297)
  AUTHORS   Pan,T.C., Zhang,R.Z., Arita,M., Bogdanovich,S., Adams,S.M.,
            Gara,S.K., Wagener,R., Khurana,T.S., Birk,D.E. and Chu,M.L.
  TITLE     A mouse model for dominant collagen VI disorders: heterozygous
            deletion of Col6a3 Exon 16
  JOURNAL   J Biol Chem 289 (15), 10293-10307 (2014)
   PUBMED   24563484
REFERENCE   5  (bases 1 to 9297)
  AUTHORS   Naba,A., Clauser,K.R., Hoersch,S., Liu,H., Carr,S.A. and Hynes,R.O.
  TITLE     The matrisome: in silico definition and in vivo characterization by
            proteomics of normal and tumor extracellular matrices
  JOURNAL   Mol Cell Proteomics 11 (4), M111.014647 (2012)
   PUBMED   22159717
REFERENCE   6  (bases 1 to 9297)
  AUTHORS   Gara,S.K., Grumati,P., Squarzoni,S., Sabatelli,P., Urciuolo,A.,
            Bonaldo,P., Paulsson,M. and Wagener,R.
  TITLE     Differential and restricted expression of novel collagen VI chains
            in mouse
  JOURNAL   Matrix Biol 30 (4), 248-257 (2011)
   PUBMED   21477648
  REMARK    GeneRIF: The collagen VI alpha 5 chain is present in perimysium and
            at the neuromuscular junctions in skeletal muscle, skin, kidney
            glomerulus, interfollicular stroma in ovary and in the tunica
            albuginea of testis
REFERENCE   7  (bases 1 to 9297)
  AUTHORS   Fitzgerald,J., Rich,C., Zhou,F.H. and Hansen,U.
  TITLE     Three novel collagen VI chains, alpha4(VI), alpha5(VI), and
            alpha6(VI)
  JOURNAL   J Biol Chem 283 (29), 20170-20180 (2008)
   PUBMED   18400749
  REMARK    GeneRIF: discovery of three additional collagen VI chains doubles
            the collagen VI family and adds a layer of complexity to collagen
            VI assembly and function in the extracellular matrix
REFERENCE   8  (bases 1 to 9297)
  AUTHORS   Gara,S.K., Grumati,P., Urciuolo,A., Bonaldo,P., Kobbe,B., Koch,M.,
            Paulsson,M. and Wagener,R.
  TITLE     Three novel collagen VI chains with high homology to the alpha3
            chain
  JOURNAL   J Biol Chem 283 (16), 10658-10670 (2008)
   PUBMED   18276594
  REMARK    GeneRIF: analysis of collagen VI chains alpha4, alpha5, and
            alpha6with high homology to the alpha3 chain
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AM748256.1, AM748257.1, AC120386.12, AM748258.1 and AK134435.1.
            
            On or before Nov 21, 2009 this sequence version replaced
            XM_909153.3, XR_035671.1.
            
            ##Evidence-Data-START##
            RNAseq introns :: mixed sample support SAMN00849374, SAMN00849375
                              [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-3762              AM748256.1         1-3762
            3763-4572           AM748257.1         499-1308
            4573-4573           AC120386.12        184972-184972
            4574-6778           AM748257.1         1310-3514
            6779-8298           AM748258.1         593-2112
            8299-8400           AK134435.1         430-531
            8401-8450           AK134435.1         533-582
            8451-9297           AK134435.1         584-1430
FEATURES             Location/Qualifiers
     source          1..9297
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="9"
                     /map="9 57.34 cM"
     gene            1..9297
                     /gene="Col6a5"
                     /gene_synonym="Col29a1; EG665033; Gm7455"
                     /note="collagen, type VI, alpha 5"
                     /db_xref="GeneID:665033"
                     /db_xref="MGI:MGI:3648134"
     exon            1..98
                     /gene="Col6a5"
                     /gene_synonym="Col29a1; EG665033; Gm7455"
                     /inference="alignment:Splign:2.1.0"
     exon            99..193
                     /gene="Col6a5"
                     /gene_synonym="Col29a1; EG665033; Gm7455"
                     /inference="alignment:Splign:2.1.0"
     CDS             127..8049
                     /gene="Col6a5"
                     /gene_synonym="Col29a1; EG665033; Gm7455"
                     /note="collagen alpha-1(XXIX) chain; collagen alpha-5(VI)
                     chain"
                     /codon_start=1
                     /product="collagen alpha-5(VI) chain precursor"
                     /protein_id="NP_001161395.1"
                     /db_xref="CCDS:CCDS85720.1"
                     /db_xref="GeneID:665033"
                     /db_xref="MGI:MGI:3648134"
                     /translation="MKLRLIAFVLILWTETLADQSPGPGPEYADVVFLVDSSNYLGIK
                     SFPFVRTFLNRMISSLPIEANKYRVALAQYSDALHNEFQLGTFKNRNPMLNHLKKNFG
                     FIGGSLKIGNALQEAHRTYFSAPTNGRDKKQFPPILVVLASAESEDDVEEAAKALRED
                     GVKIISVGVQKASEENLKAMATSQFHFNLRTARDLGMFAPNMTRIIKDVTQYREGTTV
                     DLITAVAPTTPAAPATPAAPTIPAALTTAANHVDKTVPFPTSCQKDSLADLIFLVDES
                     VGTTQNLRDLQNFLENVTSSVDVKDNCMRLGLMSFSDRAQTISSLRSSANQSEFQQQI
                     QKLSLQTGASNVGAAIEQMRKEGFSESSGSRKAQGVPQIAVLVTHRASDDMVREAALD
                     LRLEGVTMFAMGIEGANNTQLEDIVSYPSRQSISTHSSYSHLESYSGNFLKKIRNEIW
                     TQVSTRAEQMELDKTGCVDTKEADIYFLIDGSSSIRKKEFEQIQIFMSSVIDMFPIGP
                     NKVRVGVVQYSHKNEVEFPVSRYTDGIDLKKAVFNIKQLKGLTFTGKALDFILPLIKK
                     GKTERTDRAPCYLIVLTDGKSNDSVLEPANRLRAEQITIHAIGIGEANKTQLRQIAGK
                     DERVNFGQNFDSLKSIKNEIVHRICSEKGCEDMKADIMFLVDSSGSIGPTNFETMKTF
                     MKNLVGKIQIGADRSQVGVVQFSDYNREEFQLNKYSTHEEIYAAIDRMSPINRNTLTG
                     GALTFVNEYFDLSKGGRPQVRKFLILLTDGKAQDEVGGPATALRSKSVTIFSVGVYGA
                     NRAQLEEISGDGSLVFHVENFDHLKAIESKLIFRVCALHDCKRIELLDIVFVLDHSGS
                     IGPREQESMMNLTIHLVKKADVGRDRVQIGALTYSNHPEILFYLNTYSSGSAIAEHLR
                     RPRDTGGETYTAKALQHSNVLFTEEHGSRLTQNVRQLMIVITDGVSHDRDKLDEAARE
                     LRDKGITIFAVGVGNANQDELETMAGKKENTVHVDNFDKLRDIYLPLQETLCNNSQET
                     CNLPEADVIFLCDGSDMVSDSEFVTMTTFLSDLIDNFDIESQRMKIGMAQYGSRYQEI
                     IELESSLNKTQWKSQVHSVAQSKGLPRLDFALKHVSDMFDPSVGGRRNAGVPQTLVVI
                     TSSSPRYDVTDAVKVLKDLGICVLALGIGDVYKEQLLPITGNSEKIITFRDFNKLKNV
                     DVKKRMVREICQSCGKANCFVDVVVGFDISTHRQGQPLFQGHPRLESYLPGILEDITS
                     IRGVSCGAGAEAQVSLAFKVNSDQEFPAKFQIYQKAAFDSLLHVTVRGPTHLDAPFLQ
                     SLWDMFEERSASRGQVLLIFSDGLQGESITLLERQSDRLREAGLDALLVVSLNTFGHD
                     EFSSFEFGKGFDYRTQLTIGMLDLGKTLSQYLGNIAERACCCTFCKCPGIPGPHGTRG
                     LQASKGSSGPKGSRGHRGEDGDPGRRGEIGLQGDRGVVGCPGTRGQKGVKGFSGAQGE
                     HGEDGLDGLDGEEGFYGFRGGKGQKGDPGNQGYPGIRGAAGEDGEKGFPGDPGDPGKD
                     SNIKGQKGEKGERGRQGITGQKGTHGRPSSKGSRGMEGQRGPQGPSGQAGNPGPQGTQ
                     GPEGLQGSQGSSGNRGGKGDKGSQGYQGPQGSPGPAGPRGDIGRPGFGGRKGEPGVPG
                     GPGPVGPPGQRGKQGDYGIPGYGQTGRKGVKGPTGFPGDPGQKGDAGNPGIPGGPGPK
                     GFKGLTLSQGLKGRSGLQGSQGPPGRRGPKGTAGQPIYSPCELIQFLRDHSPCWKDKC
                     PVYPTELVFALDQSSGITERRFNETRDTITSIVSDLNIRENNCPVGARVAVVSYDSDT
                     SYLIRGSDYHNKKHLLQLLSQIKYQVPRKARDIGNAMRFVARNVFKRMSAGTNTRRVA
                     VFFSNGQAASRASILTATMELSALDISLAVFAYNERVFLDEAFGFDDTGTFQVIPVPP
                     VGDYEPLEKLRRCTLCYDKCFPNTCAEEPFFPENSYMDVAFLLDNSKNIASDDFQAVK
                     ALVSSVIDSFHITSNPSASESGDRVALLSYSPSESSRRKGRVKTEFAFTTYDNQSIMK
                     NYIYTSLQQLNGDATIGLALQWAMEGLFLGTPNPRKHKVIIVISAGENHEEKEFVKTV
                     ALRAKCQGYVVFVISLGSTQRDEMEELASYPLDHHLIQLGRMYKPDLNYIVKFLKPFI
                     YSVRRGFNQYPPPTLKDDCRLVELERGDTLPHGLRLTAKLREVPESTISLADQELNAG
                     KDSSFVLEDHRGDHLVYVPSQMLEPHKLVSHYGNDRESVAMASLTSEHESHGREELGL
                     AHEPGDASLQEYYMDVAFLIDASQRVGGRNEFKEVRTLITSVLDYFHIAPAPLTSVLG
                     DRVAVLTYSPPGYLPNTEECPVYLEFDLVTYNTVHQMKHHLQESLQQLNGDVFIGHAL
                     QWTVDNVFVGTPNLRKNKVIFIVTAGETNPLDKEVLRNASLRAKCQGYSIFVFSFGPI
                     HNDMELEELASHPLDHHLVRLGRVHRPDLDYVIKFIKPFVHSIRRAINKYPGRDLQAK
                     CDNLTFPGPENAGTEDSALLIPEVYRIEAGENELSGDSGSQEQHFFLLGNSHGNHSES
                     TADLMRQLYLLLSSGELMVNDKEEPCSAETPAPVNSKQDGEDAR"
     exon            194..889
                     /gene="Col6a5"
                     /gene_synonym="Col29a1; EG665033; Gm7455"
                     /inference="alignment:Splign:2.1.0"
     exon            890..1522
                     /gene="Col6a5"
                     /gene_synonym="Col29a1; EG665033; Gm7455"
                     /inference="alignment:Splign:2.1.0"
     exon            1523..2083
                     /gene="Col6a5"
                     /gene_synonym="Col29a1; EG665033; Gm7455"
                     /inference="alignment:Splign:2.1.0"
     exon            2084..2638
                     /gene="Col6a5"
                     /gene_synonym="Col29a1; EG665033; Gm7455"
                     /inference="alignment:Splign:2.1.0"
     exon            2639..3214
                     /gene="Col6a5"
                     /gene_synonym="Col29a1; EG665033; Gm7455"
                     /inference="alignment:Splign:2.1.0"
     exon            3215..3790
                     /gene="Col6a5"
                     /gene_synonym="Col29a1; EG665033; Gm7455"
                     /inference="alignment:Splign:2.1.0"
     exon            3791..4131
                     /gene="Col6a5"
                     /gene_synonym="Col29a1; EG665033; Gm7455"
                     /inference="alignment:Splign:2.1.0"
     exon            4132..4213
                     /gene="Col6a5"
                     /gene_synonym="Col29a1; EG665033; Gm7455"
                     /inference="alignment:Splign:2.1.0"
     exon            4214..4356
                     /gene="Col6a5"
                     /gene_synonym="Col29a1; EG665033; Gm7455"
                     /inference="alignment:Splign:2.1.0"
     exon            4357..4449
                     /gene="Col6a5"
                     /gene_synonym="Col29a1; EG665033; Gm7455"
                     /inference="alignment:Splign:2.1.0"
     exon            4450..4503
                     /gene="Col6a5"
                     /gene_synonym="Col29a1; EG665033; Gm7455"
                     /inference="alignment:Splign:2.1.0"
     exon            4504..4575
                     /gene="Col6a5"
                     /gene_synonym="Col29a1; EG665033; Gm7455"
                     /inference="alignment:Splign:2.1.0"
     exon            4576..4602
                     /gene="Col6a5"
                     /gene_synonym="Col29a1; EG665033; Gm7455"
                     /inference="alignment:Splign:2.1.0"
     exon            4603..4647
                     /gene="Col6a5"
                     /gene_synonym="Col29a1; EG665033; Gm7455"
                     /inference="alignment:Splign:2.1.0"
     exon            4648..4701
                     /gene="Col6a5"
                     /gene_synonym="Col29a1; EG665033; Gm7455"
                     /inference="alignment:Splign:2.1.0"
     exon            4702..4764
                     /gene="Col6a5"
                     /gene_synonym="Col29a1; EG665033; Gm7455"
                     /inference="alignment:Splign:2.1.0"
     exon            4765..4830
                     /gene="Col6a5"
                     /gene_synonym="Col29a1; EG665033; Gm7455"
                     /inference="alignment:Splign:2.1.0"
     exon            4831..4884
                     /gene="Col6a5"
                     /gene_synonym="Col29a1; EG665033; Gm7455"
                     /inference="alignment:Splign:2.1.0"
     exon            4885..4920
                     /gene="Col6a5"
                     /gene_synonym="Col29a1; EG665033; Gm7455"
                     /inference="alignment:Splign:2.1.0"
     exon            4921..4983
                     /gene="Col6a5"
                     /gene_synonym="Col29a1; EG665033; Gm7455"
                     /inference="alignment:Splign:2.1.0"
     exon            4984..5046
                     /gene="Col6a5"
                     /gene_synonym="Col29a1; EG665033; Gm7455"
                     /inference="alignment:Splign:2.1.0"
     exon            5047..5109
                     /gene="Col6a5"
                     /gene_synonym="Col29a1; EG665033; Gm7455"
                     /inference="alignment:Splign:2.1.0"
     exon            5110..5172
                     /gene="Col6a5"
                     /gene_synonym="Col29a1; EG665033; Gm7455"
                     /inference="alignment:Splign:2.1.0"
     exon            5173..5223
                     /gene="Col6a5"
                     /gene_synonym="Col29a1; EG665033; Gm7455"
                     /inference="alignment:Splign:2.1.0"
     exon            5224..5259
                     /gene="Col6a5"
                     /gene_synonym="Col29a1; EG665033; Gm7455"
                     /inference="alignment:Splign:2.1.0"
     exon            5260..5322
                     /gene="Col6a5"
                     /gene_synonym="Col29a1; EG665033; Gm7455"
                     /inference="alignment:Splign:2.1.0"
     exon            5323..5385
                     /gene="Col6a5"
                     /gene_synonym="Col29a1; EG665033; Gm7455"
                     /inference="alignment:Splign:2.1.0"
     exon            5386..5421
                     /gene="Col6a5"
                     /gene_synonym="Col29a1; EG665033; Gm7455"
                     /inference="alignment:Splign:2.1.0"
     exon            5422..5458
                     /gene="Col6a5"
                     /gene_synonym="Col29a1; EG665033; Gm7455"
                     /inference="alignment:Splign:2.1.0"
     exon            5459..5470
                     /gene="Col6a5"
                     /gene_synonym="Col29a1; EG665033; Gm7455"
                     /inference="alignment:Splign:2.1.0"
     exon            5471..5958
                     /gene="Col6a5"
                     /gene_synonym="Col29a1; EG665033; Gm7455"
                     /inference="alignment:Splign:2.1.0"
     exon            5959..6052
                     /gene="Col6a5"
                     /gene_synonym="Col29a1; EG665033; Gm7455"
                     /inference="alignment:Splign:2.1.0"
     exon            6053..6709
                     /gene="Col6a5"
                     /gene_synonym="Col29a1; EG665033; Gm7455"
                     /inference="alignment:Splign:2.1.0"
     exon            6710..6800
                     /gene="Col6a5"
                     /gene_synonym="Col29a1; EG665033; Gm7455"
                     /inference="alignment:Splign:2.1.0"
     exon            6801..7009
                     /gene="Col6a5"
                     /gene_synonym="Col29a1; EG665033; Gm7455"
                     /inference="alignment:Splign:2.1.0"
     exon            7010..7699
                     /gene="Col6a5"
                     /gene_synonym="Col29a1; EG665033; Gm7455"
                     /inference="alignment:Splign:2.1.0"
     exon            7700..7796
                     /gene="Col6a5"
                     /gene_synonym="Col29a1; EG665033; Gm7455"
                     /inference="alignment:Splign:2.1.0"
     exon            7797..8029
                     /gene="Col6a5"
                     /gene_synonym="Col29a1; EG665033; Gm7455"
                     /inference="alignment:Splign:2.1.0"
     exon            8030..9297
                     /gene="Col6a5"
                     /gene_synonym="Col29a1; EG665033; Gm7455"
                     /inference="alignment:Splign:2.1.0"
     regulatory      8404..8409
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Col6a5"
                     /gene_synonym="Col29a1; EG665033; Gm7455"
                     /note="hexamer: AGTAAA"
     polyA_site      8427
                     /gene="Col6a5"
                     /gene_synonym="Col29a1; EG665033; Gm7455"
                     /note="major polyA site"
ORIGIN      
        1 ggagcagcag gaacccagca gcgaggtgca tgctccaagg ggacacaggg ctgcactgcg
       61 ggctgcccca ggatcctcgg ggaccccagg aagtcttgaa taaaaataag aaaccccgtt
      121 aacagaatga agctccggct aattgcgttt gtcttaatcc tctggactga aaccctggca
      181 gaccagagcc cagggccagg ccccgagtac gcagacgtgg tgtttctggt ggacagctcc
      241 aattacctgg gaattaagtc cttcccattt gtgagaactt ttctcaacag gatgatcagc
      301 agcctcccca tagaggccaa caagtaccgc gtggccctgg cccagtacag cgatgctctc
      361 cacaatgagt tccagctggg caccttcaag aacaggaacc ccatgctgaa ccacctgaag
      421 aagaacttcg ggttcatcgg tggctccctg aagataggga acgccctgca ggaggctcac
      481 aggacctatt tctctgctcc cacaaatggg agagacaaga aacagttccc cccaatcctg
      541 gtggtgctgg cttcagcaga gtctgaggat gatgtggaag aggctgcgaa ggccctgcgg
      601 gaagatgggg tgaaaatcat ctctgtgggg gtgcagaagg cttctgagga aaacctgaag
      661 gcgatggcca cctctcagtt ccatttcaat ctcaggactg ccagagacct cggcatgttt
      721 gccccaaaca tgacacggat catcaaggat gtgactcagt acagggaagg gacaactgtg
      781 gacttaatca cagctgtagc cccaaccaca cctgcagccc cagccacacc tgcagcccca
      841 accatacctg cagctctaac cacagctgcc aaccacgttg ataagacagt tccctttccc
      901 acctcttgcc agaaggactc ccttgccgac cttatattct tggtggatga gtcagtgggg
      961 accacacaga atctgaggga cctgcagaac ttcctggaga atgtcaccag ctctgtggat
     1021 gtgaaggaca actgcatgcg gcttgggctg atgagcttca gcgacagggc tcagactatt
     1081 tcttccctga ggtcaagcgc caaccagtct gaattccagc agcaaatcca gaaactctct
     1141 ctccagactg gggcatctaa tgttggggcc gccattgagc agatgaggaa agaaggcttc
     1201 tcagagtcca gtggcagcag gaaggcccag ggcgtccccc agattgctgt gctggtcacc
     1261 cacagagcat cagatgacat ggtgcgggag gctgcactgg atctgcgctt ggagggtgta
     1321 actatgtttg caatgggcat cgaaggggcc aacaacacgc aattagaaga catagtgtca
     1381 tacccttcca ggcagagcat ttccacacac agctcctaca gtcacttgga atcatacagt
     1441 gggaacttcc tgaagaagat ccgcaatgaa atctggaccc aagtttctac tcgtgctgaa
     1501 caaatggagc tggataaaac cggctgtgtg gatacaaagg aggccgacat ctattttctc
     1561 attgatgggt cgtccagcat ccgtaagaaa gagtttgagc aaatccagat atttatgtca
     1621 tctgtgatag acatgtttcc tatcggcccc aacaaagttc gggtcggagt tgtgcagtat
     1681 tcacataaga atgaagtgga atttcctgtc tctcgctaca cggatggcat tgacttgaaa
     1741 aaggctgttt ttaacatcaa gcaactcaaa ggtcttacat tcaccgggaa agccctggat
     1801 ttcatattgc ctctcataaa gaagggaaaa acagagagga ccgacagagc cccctgttac
     1861 ctcatcgtgc tgactgatgg aaagtcaaat gacagcgttc tcgaacctgc caacagactg
     1921 cgggctgagc agatcaccat tcacgccatc gggattggcg aggcaaacaa aacacagctg
     1981 cggcagattg ctgggaagga cgaaagggtt aacttcgggc agaacttcga ttctctgaag
     2041 agcataaaaa atgaaatcgt tcaccgcatc tgctctgaaa aaggatgtga agacatgaag
     2101 gcagacatca tgtttttggt ggacagttct ggaagcatag ggcctacaaa ttttgagaca
     2161 atgaaaactt tcatgaaaaa tctggtgggg aagattcaga ttggcgccga cagatcccaa
     2221 gtcggtgttg ttcaattcag tgactataac agggaagaat tccagcttaa taaatactcc
     2281 acgcatgagg aaatttatgc agccatagat aggatgtctc ccatcaacag aaacactctg
     2341 acaggaggag cactaacctt tgtaaatgaa tactttgacc tttccaaggg ggggcgcccc
     2401 caggtcagaa agttccttat cctcctcaca gatggaaaag ctcaggacga agtggggggc
     2461 ccagccacgg ctctcaggag caaaagtgtg accatcttct ccgtgggtgt gtatggggcc
     2521 aatagggctc agctggagga gatcagtgga gatggcagtc tggttttcca tgttgagaac
     2581 tttgaccatc tgaaggccat agagagtaaa ctcatctttc gagtgtgtgc tctgcatgat
     2641 tgcaagagga tcgaactctt agacatcgtg tttgtgctgg accactcagg cagcataggt
     2701 ccacgtgagc aagaaagcat gatgaacctc accatccact tggtgaagaa ggcagacgtt
     2761 ggtagggacc gagtgcagat tggagcgctc acatactcca accatcccga gatcctcttc
     2821 tatcttaaca cttactcaag tggatcagcc attgctgagc atctgaggag gcccagggac
     2881 accggagggg agacctacac tgccaaggct cttcagcatt ccaacgtcct gttcacagaa
     2941 gagcatggca gccgcctgac gcagaacgtg aggcagctga tgattgtcat cacggatggc
     3001 gtgtcccacg accgagataa actcgacgag gcagcacggg aactcagaga caaaggcatc
     3061 accatctttg cagtgggtgt aggaaatgcc aaccaagatg aactggagac catggcaggg
     3121 aagaaagaaa acactgtcca tgtagacaac tttgacaaac tgagagatat ttacctgcct
     3181 ctgcaagaga ctctgtgtaa taattcacaa gagacttgta accttccgga agctgatgtg
     3241 attttccttt gtgatggctc tgacatggtg tctgattcag agttcgttac catgacaact
     3301 ttcttgtcag acttaattga taattttgac attgagtctc aaagaatgaa gattgggatg
     3361 gctcaatacg ggagccggta tcaggaaatt attgagttgg aaagctctct gaacaagact
     3421 cagtggaagt ctcaggtcca cagtgtggcc cagagcaagg ggttgccacg ccttgacttt
     3481 gctctcaagc atgtgagtga catgtttgat ccatccgttg gcgggagaag gaacgctggt
     3541 gtccctcaga ctctggttgt tataacatct agttctccac gctatgatgt gacagacgcg
     3601 gtaaaggtcc tgaaagacct tggcatctgt gtcctggctt tgggcatagg ggatgtttat
     3661 aaggaacagc ttctgcccat cacaggcaat tcagaaaaga taattacttt ccgagacttt
     3721 aataagctaa agaatgtgga tgtgaagaag agaatggttc gtgaaatttg ccagagctgt
     3781 gggaaagcca attgctttgt ggatgtagtg gtcgggttcg acatttccac tcaccgtcag
     3841 ggccagcctt tgttccaggg ccatccccgc ttagaatcct acctcccggg catcttggag
     3901 gacatcacct ctatcagagg ggtgagctgt ggtgcaggtg cagaggcgca ggtgagcctg
     3961 gcctttaagg tgaacagtga ccaagagttc ccagccaagt tccagatcta tcagaaagca
     4021 gcgtttgaca gcttgttgca cgtcacagtc cgtggaccaa ctcatctgga tgcaccgttc
     4081 ttgcagtcac tttgggacat gtttgaggaa agatctgcat ccaggggcca ggttctgctc
     4141 atcttctcag acggtctcca gggtgaaagc atcaccttgc tggagcggca gtcagacagg
     4201 ctcagagaag caggacttga tgctctgttg gtggtgtccc ttaacacatt cggtcacgat
     4261 gagttttcca gctttgaatt tggaaaagga tttgactaca ggactcagct gaccattggg
     4321 atgctagacc tgggcaagac gctctcacag tacctgggaa acatcgcaga aagggcttgc
     4381 tgctgtacat tttgtaaatg tccggggatt ccaggacctc atggaacccg gggactccaa
     4441 gcctcaaagg gttcttcagg tccaaaaggt agcagaggac acaggggaga ggacggagac
     4501 cctggaagac gaggagaaat tgggcttcaa ggagacagag gagtcgtggg atgcccgggg
     4561 actcgaggtc aaaagggagt caaaggattt tcaggagctc agggggaaca tggagaggac
     4621 gggctggacg gactggatgg agaagaagga ttttacggat ttcgtggggg aaagggacaa
     4681 aaaggtgacc ctggcaatca gggttaccca ggcatcagag gcgctgctgg ggaagatggc
     4741 gagaagggct tcccagggga tcccggtgac ccaggaaaag acagcaacat caaaggccag
     4801 aagggcgaga aaggagaacg aggaagacaa gggataactg ggcagaaggg gacacatggc
     4861 cgtcctagct ccaaaggaag caggggaatg gagggccaga gggggcccca gggtccctca
     4921 gggcaggcag gaaacccagg acctcaaggt acacaaggac ctgaaggact acaaggctca
     4981 cagggatcaa gtggaaatcg tggagggaaa ggagacaaag gaagtcaagg ttatcaggga
     5041 cctcagggtt ctcctgggcc agcaggaccc agaggggata ttggaagacc tggatttggg
     5101 gggagaaagg gggaacctgg agttcctgga ggcccaggac cagtggggcc ccctggacag
     5161 cgaggaaagc agggagatta tggcatcccg ggctatggtc agacaggaag aaaaggagtt
     5221 aagggcccaa caggattccc tggggaccca gggcagaagg gtgacgctgg caatcctgga
     5281 attcctgggg gacctggacc caaaggattc aagggactga ctctctctca aggcttgaaa
     5341 ggcaggagtg gacttcaggg gtctcaaggc cctcctggtc ggagaggtcc taaaggcaca
     5401 gcagggcaac ctatatattc cccctgtgaa ctgatccagt tcctgaggga ccacagcccc
     5461 tgttggaaag acaagtgccc tgtgtatcca acagagctgg tctttgctct ggaccagtcc
     5521 tctggtatca cagaaaggag atttaatgaa acaagggaca ccatcacttc aattgtcagt
     5581 gacctcaaca tcagggagaa caactgtccc gtgggagcac gggttgccgt ggtctcctat
     5641 gactcagaca ccagctatct catccgtggc tctgactacc ataacaagaa gcatctccta
     5701 cagcttcttt cccagataaa ataccaagtg ccccggaagg ccagggacat tggcaatgca
     5761 atgaggtttg tggcccgcaa cgttttcaag cggatgtctg cgggaaccaa caccaggaga
     5821 gtggctgtgt ttttcagcaa tggccaggca gccagtagag catccatcct cacggccacc
     5881 atggagttga gtgccctcga tatcagcctt gccgtctttg cttataacga aagagtgttc
     5941 cttgatgagg cttttgggtt tgatgacact gggacatttc aggtgattcc agtcccccca
     6001 gttggagatt atgagccact ggagaagctt cggcgctgca cactttgcta tgacaagtgt
     6061 tttccaaaca cctgtgcaga agagcctttc tttcctgaga attcctacat ggatgttgca
     6121 ttcctcttag acaattccaa aaacatcgcc agcgatgact tccaagccgt gaaagcgctg
     6181 gtgagctcag tgattgacag cttccacatc acttcaaacc cttcagcctc agagtctggt
     6241 gacagggttg ctttgctgag ctattctccc tcagagagct ccagaaggaa gggcagggtg
     6301 aagacagagt ttgcctttac aacctacgac aaccaatcaa tcatgaagaa ctacatctac
     6361 acttcccttc aacagctcaa tggagatgct actattggcc ttgccctaca gtgggccatg
     6421 gaaggtctct tcctgggaac ccccaatcca agaaaacaca aggtcatcat tgtgatttca
     6481 gctggagaaa accatgagga gaaggaattt gtgaagacgg tggctttgag ggccaaatgt
     6541 caaggctatg tcgtatttgt gatatctctg ggctccacac aaagagatga gatggaagaa
     6601 ttagccagct acccactcga tcaccatctg atacagcttg ggagaatgta caagccagat
     6661 ctgaattata ttgtgaaatt tctgaagccc tttatatact cggtcagacg cggatttaat
     6721 caatacccac cacccacgct gaaggacgac tgcagacttg ttgagttaga gaggggagac
     6781 acactgcccc atggcctcag attgactgct aagctgcgtg aggttcctga gagcacaatc
     6841 agcctggctg atcaagagct gaacgctggg aaagactcat cttttgtgtt ggaggatcac
     6901 agaggcgacc atttggttta cgttccaagc cagatgctag agccacataa gttagtgagc
     6961 cactatggaa atgaccggga atctgtagca atggcgagtc tcacttctga acatgaaagt
     7021 catggcagag aagaactggg tcttgctcat gagcctgggg atgcttctct tcaagaatat
     7081 tacatggacg tggctttcct catagatgct tctcaacgag taggaggacg taacgagttt
     7141 aaggaagtga gaactttgat aacgtcagta ctggattact ttcacattgc cccagctcca
     7201 ctgacctctg tcttaggaga cagagtggct gtcctgacct actctccccc aggctatctg
     7261 ccaaacactg aagagtgccc agtctacctg gaattcgatt tggtcactta caacactgta
     7321 catcaaatga agcatcatct ccaagagtct cttcagcagc tcaatggtga tgtttttatt
     7381 gggcatgccc ttcagtggac agttgacaat gtctttgtag gaacccccaa tctgaggaaa
     7441 aacaaagtta tctttatagt aactgcaggt gaaaccaacc ctttagacaa agaagtttta
     7501 aggaatgcat ctctgagagc caagtgccaa ggctactcca tatttgtatt ttcctttggt
     7561 ccgatacaca atgacatgga gttagaagaa ttagcaagcc accctctgga tcatcactta
     7621 gtccggcttg gccgggtaca caggccagat ttggactatg tcatcaagtt catcaagcca
     7681 tttgttcatt cgatcagacg tgctatcaac aaatatcccg gcagagacct gcaagccaag
     7741 tgtgataatc tcaccttccc tggcccagag aatgctggca cagaagacag tgcattactc
     7801 atccccgagg tgtacagaat cgaggcagga gagaatgagc tgtctggaga ctctggatct
     7861 caggagcagc atttcttcct cctagggaac agtcatggca accattcaga aagcactgct
     7921 gatctgatgc ggcagttgta cctgctcctc tcctccgggg aactgatggt gaatgataag
     7981 gaagagcctt gttccgcaga gaccccagct ccagtgaaca gcaaacaaga tggcgaagat
     8041 gcaagatgag ctttcaggag agaagagtgc tcgctggcag atgggactcc cctgcggcag
     8101 tagttccagc cagaaaatgc caatcattta ttagaagggt cggggcagga actagaacta
     8161 aatttgcttc tcagtcccaa acctgaggga gagactcttc aggatagaga catcaaggaa
     8221 cagaccttca aaactaggac cgggtttcat ctctcaggag gaatgggacc actgagcaag
     8281 aagaggcaac agccttcccc gtgacagctt ggttcactcg ctttgatcac tttggtgagt
     8341 gcctgccaaa agaagatgtt tgtcatgatc gccatcacat ctacatctca aattagaccc
     8401 ttgagtaaaa acagtttgta attggaatgt cccaattgtc tttttttttt cttcccattt
     8461 atacaaccct aaactctgtg tcgctggttc ccataggacc ctgagattac atttagcacc
     8521 cgttacctat agtcagtggg ttttctttac caggacattt atagagtaac aaattcataa
     8581 caaagaaagg ggaggagaag ctgccttgtg agctacccgc tcgtgctggg aacacttgtt
     8641 ttctatttta gtgtgctttg cattgtgtgg acttgagttt tgctgccaaa gaaatcatgc
     8701 caacatgtga acctggaaac agagctattc tcaaacagtg tatcaaaaca gactctaaaa
     8761 cagaaaaaga acaagacaat aacaacaacc ccaagtctga cccagaaact tattctgtgc
     8821 attcttatta actgtagtct cactcagccc aggaaagcac attaaaccta cttaggaaac
     8881 acacatacag gacaagacaa agcagtagtg aatttgttat acactcggtc agtttaacat
     8941 ggagtatgca ggcagtggag tactgtttag gcataaagaa gagtaaagtc atgtgacttg
     9001 tgggaagatg gacagaaccg taggcgttcg tgttgattga cataagccgg cttcatatgt
     9061 cttctcccat acgtggaatt ccaggactgg gggattaaga ggatgagaaa gtaaagcaga
     9121 tttggagggg agaggggaat aaaaggagac tcagaggagg tgaatgttat cagagtgttt
     9181 tacatgctgt ctggaaatgg cataatgaaa cccattattt tgtacaatta ttatattcta
     9241 ataaaatcac ttgatttggt taaggcaaca agtagttgcc aaaccaatga gcatgtg
//
