LOCUS       NM_007735               7808 bp    mRNA    linear   ROD 18-MAR-2025
DEFINITION  Mus musculus collagen, type IV, alpha 4 (Col4a4), mRNA.
ACCESSION   NM_007735 NM_177015
VERSION     NM_007735.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 7808)
  AUTHORS   Wang,M., Liu,H., Zhang,M., Niu,X., Sun,M., Wang,F., Ni,Y., Hong,T.,
            Zhang,W. and Dang,S.
  TITLE     The secreted protease ADAMTS18 links early isoform transformation
            and maturation of glomerular basement membrane macromolecules to
            the integrity of the glomerular filtration barrier
  JOURNAL   Biochem Biophys Res Commun 750, 151386 (2025)
   PUBMED   39879696
REFERENCE   2  (bases 1 to 7808)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 7808)
  AUTHORS   Lipp,S.N., Jacobson,K.R., Schwaderer,A.L., Hains,D.S. and Calve,S.
  TITLE     FOXD1 is required for 3D patterning of the kidney interstitial
            matrix
  JOURNAL   Dev Dyn 252 (4), 463-482 (2023)
   PUBMED   36335435
REFERENCE   4  (bases 1 to 7808)
  AUTHORS   Aypek,H., Krisp,C., Lu,S., Liu,S., Kylies,D., Kretz,O., Wu,G.,
            Moritz,M., Amann,K., Benz,K., Tong,P., Hu,Z.M., Alsulaiman,S.M.,
            Khan,A.O., Grohmann,M., Wagner,T., Muller-Deile,J., Schluter,H.,
            Puelles,V.G., Bergmann,C., Huber,T.B. and Grahammer,F.
  TITLE     Loss of the collagen IV modifier prolyl 3-hydroxylase 2 causes thin
            basement membrane nephropathy
  JOURNAL   J Clin Invest 132 (9) (2022)
   PUBMED   35499085
REFERENCE   5  (bases 1 to 7808)
  AUTHORS   Damen,M., Wirtz,L., Soroka,E., Khatif,H., Kukat,C., Simons,B.D. and
            Bazzi,H.
  TITLE     High proliferation and delamination during skin epidermal
            stratification
  JOURNAL   Nat Commun 12 (1), 3227 (2021)
   PUBMED   34050161
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 7808)
  AUTHORS   Thorner,P.S., Zheng,K., Kalluri,R., Jacobs,R. and Hudson,B.G.
  TITLE     Coordinate gene expression of the alpha3, alpha4, and alpha5 chains
            of collagen type IV. Evidence from a canine model of X-linked
            nephritis with a COL4A5 gene mutation
  JOURNAL   J Biol Chem 271 (23), 13821-13828 (1996)
   PUBMED   8662866
REFERENCE   7  (bases 1 to 7808)
  AUTHORS   Enders,G.C., Kahsai,T.Z., Lian,G., Funabiki,K., Killen,P.D. and
            Hudson,B.G.
  TITLE     Developmental changes in seminiferous tubule extracellular matrix
            components of the mouse testis: alpha 3(IV) collagen chain
            expressed at the initiation of spermatogenesis
  JOURNAL   Biol Reprod 53 (6), 1489-1499 (1995)
   PUBMED   8562707
REFERENCE   8  (bases 1 to 7808)
  AUTHORS   Miner,J.H. and Sanes,J.R.
  TITLE     Collagen IV alpha 3, alpha 4, and alpha 5 chains in rodent basal
            laminae: sequence, distribution, association with laminins, and
            developmental switches
  JOURNAL   J Cell Biol 127 (3), 879-891 (1994)
   PUBMED   7962065
REFERENCE   9  (bases 1 to 7808)
  AUTHORS   Wu,J.E. and Santoro,S.A.
  TITLE     Complex patterns of expression suggest extensive roles for the
            alpha 2 beta 1 integrin in murine development
  JOURNAL   Dev Dyn 199 (4), 292-314 (1994)
   PUBMED   7521231
REFERENCE   10 (bases 1 to 7808)
  AUTHORS   Saga,Y., Yagi,T., Ikawa,Y., Sakakura,T. and Aizawa,S.
  TITLE     Mice develop normally without tenascin
  JOURNAL   Genes Dev 6 (10), 1821-1831 (1992)
   PUBMED   1383086
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK164732.1, AF169388.1 and AC160543.13.
            
            On Apr 25, 2007 this sequence version replaced NM_007735.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF169388.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-57                AK164732.1         1-57
            58-5136             AF169388.1         1-5079
            5137-7808           AC160543.13        83029-85700
FEATURES             Location/Qualifiers
     source          1..7808
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="1"
                     /map="1 42.2 cM"
     gene            1..7808
                     /gene="Col4a4"
                     /gene_synonym="E130010M05Rik; [a]4(IV)"
                     /note="collagen, type IV, alpha 4"
                     /db_xref="GeneID:12829"
                     /db_xref="MGI:MGI:104687"
     exon            1..231
                     /gene="Col4a4"
                     /gene_synonym="E130010M05Rik; [a]4(IV)"
                     /inference="alignment:Splign:2.1.0"
     exon            232..398
                     /gene="Col4a4"
                     /gene_synonym="E130010M05Rik; [a]4(IV)"
                     /inference="alignment:Splign:2.1.0"
     CDS             352..5400
                     /gene="Col4a4"
                     /gene_synonym="E130010M05Rik; [a]4(IV)"
                     /note="collagen alpha-4(IV) chain; procollagen, type IV,
                     alpha 4"
                     /codon_start=1
                     /product="collagen alpha-4(IV) chain precursor"
                     /protein_id="NP_031761.1"
                     /db_xref="CCDS:CCDS35630.1"
                     /db_xref="GeneID:12829"
                     /db_xref="MGI:MGI:104687"
                     /translation="MRCFFRWTKSFVTAPWSLIFILFTIQYEYGSGKKYGGPCGGRNC
                     SVCQCFPEKGSRGHPGPLGPQGPIGPLGPLGPIGIPGEKGERGDSGSPGPPGEKGDKG
                     PTGVPGFPGVDGVPGHPGPPGPRGKPGVDGYNGSRGDPGYPGERGAPGPGGPPGQPGE
                     NGEKGRSVYITGGVKGIQGDRGDPGPPGLPGSRGAQGSPGPMGHAGAPGLAGPIGHPG
                     SPGLKGNPATGLKGQRGEPGEVGQRGPPGPTLLVQPPDLSIYKGEKGVKGMPGMIGPP
                     GPPGRKGAPGVGIKGEKGIPGFPGPRGEPGSHGPPGFPGFKGIQGAAGEPGLFGFLGP
                     KGDLGDRGYPGPPGILLTPAPPLKGVPGDPGPPGYYGEIGDVGLPGPPGPPGRPGETC
                     PGMMGPPGPPGVPGPPGFPGEAGVPGRLDCAPGKPGKPGLPGLPGAPGPEGPPGSDVI
                     YCRPGCPGPMGEKGKVGPPGRRGAKGAKGNKGLCTCPPGPMGPPGPPGPPGRQGSKGD
                     LGLPGWHGEKGDPGQPGAEGPPGPPGRPGAMGPPGHKGEKGDMVISRVKGQKGERGLD
                     GPPGFPGPHGQDGGDGRPGERGDPGPRGDHKDAAPGERGLPGLPGPPGRTGPEGPPGL
                     GFPGPPGQRGLPGEPGRPGTRGFDGTKGQKGDSILCNVSYPGKPGLPGLDGPPGLKGF
                     PGPPGAPGMRCPDGQKGQRGKPGMSGIPGPPGFRGDMGDPGIKGEKGTSPIGPPGPPG
                     SPGKDGQKGIPGDPAFGDPGPPGERGLPGAPGMKGQKGHPGCPGAGGPPGIPGSPGLK
                     GPKGREGSRGFPGIPGSPGHSCERGAPGIPGQPGLPGTPGDPGAPGWKGQPGDMGPSG
                     PAGMKGLPGLPGLPGADGLRGPPGIPGPNGEDGLPGLPGLKGLPGLPGFPGFPGERGK
                     PGPDGEPGRKGEVGEKGWPGLKGDLGERGAKGDRGLPGDAGEAVTSRKGEPGDAGPPG
                     DGGFSGERGDKGSSGMRGGRGDPGRDGLPGLHRGQPGIDGPPGPPGPPGPPGSPGLRG
                     VIGFPGFPGDQGDPGSPGPPGFPGDDGARGPKGYKGDPASQCGPPGPKGEPGSPGYQG
                     RTGVPGEKGFPGDEGPRGPPGRPGQPGSFGPPGCPGDPGMPGLKGHPGEVGDPGPRGD
                     AGDFGRPGPAGVKGPLGSPGLNGLHGLKGEKGTKGASGLLEMGPPGPMGMPGQKGEKG
                     DPGSPGISPPGLPGEKGFPGPPGRPGPPGPAGAPGRAAKGDIPDPGPPGDRGPPGPDG
                     PRGVPGPPGSPGNVDLLKGDPGDCGLPGPPGSRGPPGPPGCQGPPGCDGKDGQKGPMG
                     LPGLPGPPGLPGAPGEKGLPGPPGRKGPVGPPGCRGEPGPPADVDSCPRIPGLPGVPG
                     PRGPEGAMGEPGRRGLPGPGCKGEPGPDGRRGQDGIPGSPGPPGRKGDTGEAGCPGAP
                     GPPGPTGDPGPKGFGPGSLSGFLLVLHSQTDQEPACPVGMPRLWTGYSLLYMEGQEKA
                     HNQDLGLAGSCLPVFSTLPFAYCNIHQVCHYAQRNDRSYWLSSAAPLPMMPLSEEEIR
                     SYISRCAVCEAPAQAVAVHSQDQSIPPCPRTWRSLWIGYSFLMHTGAGDQGGGQALMS
                     PGSCLEDFRAAPFVECQGRQGTCHFFANEYSFWLTTVNPDLQFASGPSPDTLKEVQAQ
                     RRKISRCQVCMKHS"
     exon            399..441
                     /gene="Col4a4"
                     /gene_synonym="E130010M05Rik; [a]4(IV)"
                     /inference="alignment:Splign:2.1.0"
     exon            442..519
                     /gene="Col4a4"
                     /gene_synonym="E130010M05Rik; [a]4(IV)"
                     /inference="alignment:Splign:2.1.0"
     exon            520..654
                     /gene="Col4a4"
                     /gene_synonym="E130010M05Rik; [a]4(IV)"
                     /inference="alignment:Splign:2.1.0"
     exon            655..699
                     /gene="Col4a4"
                     /gene_synonym="E130010M05Rik; [a]4(IV)"
                     /inference="alignment:Splign:2.1.0"
     exon            700..816
                     /gene="Col4a4"
                     /gene_synonym="E130010M05Rik; [a]4(IV)"
                     /inference="alignment:Splign:2.1.0"
     exon            817..885
                     /gene="Col4a4"
                     /gene_synonym="E130010M05Rik; [a]4(IV)"
                     /inference="alignment:Splign:2.1.0"
     exon            886..921
                     /gene="Col4a4"
                     /gene_synonym="E130010M05Rik; [a]4(IV)"
                     /inference="alignment:Splign:2.1.0"
     exon            922..984
                     /gene="Col4a4"
                     /gene_synonym="E130010M05Rik; [a]4(IV)"
                     /inference="alignment:Splign:2.1.0"
     exon            985..1020
                     /gene="Col4a4"
                     /gene_synonym="E130010M05Rik; [a]4(IV)"
                     /inference="alignment:Splign:2.1.0"
     exon            1021..1062
                     /gene="Col4a4"
                     /gene_synonym="E130010M05Rik; [a]4(IV)"
                     /inference="alignment:Splign:2.1.0"
     exon            1063..1143
                     /gene="Col4a4"
                     /gene_synonym="E130010M05Rik; [a]4(IV)"
                     /inference="alignment:Splign:2.1.0"
     exon            1144..1197
                     /gene="Col4a4"
                     /gene_synonym="E130010M05Rik; [a]4(IV)"
                     /inference="alignment:Splign:2.1.0"
     exon            1198..1257
                     /gene="Col4a4"
                     /gene_synonym="E130010M05Rik; [a]4(IV)"
                     /inference="alignment:Splign:2.1.0"
     exon            1258..1302
                     /gene="Col4a4"
                     /gene_synonym="E130010M05Rik; [a]4(IV)"
                     /inference="alignment:Splign:2.1.0"
     exon            1303..1356
                     /gene="Col4a4"
                     /gene_synonym="E130010M05Rik; [a]4(IV)"
                     /inference="alignment:Splign:2.1.0"
     exon            1357..1426
                     /gene="Col4a4"
                     /gene_synonym="E130010M05Rik; [a]4(IV)"
                     /inference="alignment:Splign:2.1.0"
     exon            1427..1531
                     /gene="Col4a4"
                     /gene_synonym="E130010M05Rik; [a]4(IV)"
                     /inference="alignment:Splign:2.1.0"
     exon            1532..1696
                     /gene="Col4a4"
                     /gene_synonym="E130010M05Rik; [a]4(IV)"
                     /inference="alignment:Splign:2.1.0"
     exon            1697..1786
                     /gene="Col4a4"
                     /gene_synonym="E130010M05Rik; [a]4(IV)"
                     /inference="alignment:Splign:2.1.0"
     exon            1787..1950
                     /gene="Col4a4"
                     /gene_synonym="E130010M05Rik; [a]4(IV)"
                     /inference="alignment:Splign:2.1.0"
     exon            1951..2023
                     /gene="Col4a4"
                     /gene_synonym="E130010M05Rik; [a]4(IV)"
                     /inference="alignment:Splign:2.1.0"
     exon            2024..2130
                     /gene="Col4a4"
                     /gene_synonym="E130010M05Rik; [a]4(IV)"
                     /inference="alignment:Splign:2.1.0"
     exon            2131..2314
                     /gene="Col4a4"
                     /gene_synonym="E130010M05Rik; [a]4(IV)"
                     /inference="alignment:Splign:2.1.0"
     exon            2315..2383
                     /gene="Col4a4"
                     /gene_synonym="E130010M05Rik; [a]4(IV)"
                     /inference="alignment:Splign:2.1.0"
     exon            2384..2491
                     /gene="Col4a4"
                     /gene_synonym="E130010M05Rik; [a]4(IV)"
                     /inference="alignment:Splign:2.1.0"
     exon            2492..2710
                     /gene="Col4a4"
                     /gene_synonym="E130010M05Rik; [a]4(IV)"
                     /inference="alignment:Splign:2.1.0"
     exon            2711..2872
                     /gene="Col4a4"
                     /gene_synonym="E130010M05Rik; [a]4(IV)"
                     /inference="alignment:Splign:2.1.0"
     exon            2873..3043
                     /gene="Col4a4"
                     /gene_synonym="E130010M05Rik; [a]4(IV)"
                     /inference="alignment:Splign:2.1.0"
     exon            3044..3187
                     /gene="Col4a4"
                     /gene_synonym="E130010M05Rik; [a]4(IV)"
                     /inference="alignment:Splign:2.1.0"
     exon            3188..3292
                     /gene="Col4a4"
                     /gene_synonym="E130010M05Rik; [a]4(IV)"
                     /inference="alignment:Splign:2.1.0"
     exon            3293..3474
                     /gene="Col4a4"
                     /gene_synonym="E130010M05Rik; [a]4(IV)"
                     /inference="alignment:Splign:2.1.0"
     exon            3475..3538
                     /gene="Col4a4"
                     /gene_synonym="E130010M05Rik; [a]4(IV)"
                     /inference="alignment:Splign:2.1.0"
     exon            3539..3613
                     /gene="Col4a4"
                     /gene_synonym="E130010M05Rik; [a]4(IV)"
                     /inference="alignment:Splign:2.1.0"
     exon            3614..3721
                     /gene="Col4a4"
                     /gene_synonym="E130010M05Rik; [a]4(IV)"
                     /inference="alignment:Splign:2.1.0"
     exon            3722..3829
                     /gene="Col4a4"
                     /gene_synonym="E130010M05Rik; [a]4(IV)"
                     /inference="alignment:Splign:2.1.0"
     exon            3830..3901
                     /gene="Col4a4"
                     /gene_synonym="E130010M05Rik; [a]4(IV)"
                     /inference="alignment:Splign:2.1.0"
     exon            3902..4030
                     /gene="Col4a4"
                     /gene_synonym="E130010M05Rik; [a]4(IV)"
                     /inference="alignment:Splign:2.1.0"
     exon            4031..4144
                     /gene="Col4a4"
                     /gene_synonym="E130010M05Rik; [a]4(IV)"
                     /inference="alignment:Splign:2.1.0"
     exon            4145..4300
                     /gene="Col4a4"
                     /gene_synonym="E130010M05Rik; [a]4(IV)"
                     /inference="alignment:Splign:2.1.0"
     exon            4301..4408
                     /gene="Col4a4"
                     /gene_synonym="E130010M05Rik; [a]4(IV)"
                     /inference="alignment:Splign:2.1.0"
     exon            4409..4417
                     /gene="Col4a4"
                     /gene_synonym="E130010M05Rik; [a]4(IV)"
                     /inference="alignment:Splign:2.1.0"
     exon            4418..4543
                     /gene="Col4a4"
                     /gene_synonym="E130010M05Rik; [a]4(IV)"
                     /inference="alignment:Splign:2.1.0"
     exon            4544..4660
                     /gene="Col4a4"
                     /gene_synonym="E130010M05Rik; [a]4(IV)"
                     /inference="alignment:Splign:2.1.0"
     exon            4661..4849
                     /gene="Col4a4"
                     /gene_synonym="E130010M05Rik; [a]4(IV)"
                     /inference="alignment:Splign:2.1.0"
     exon            4850..5136
                     /gene="Col4a4"
                     /gene_synonym="E130010M05Rik; [a]4(IV)"
                     /inference="alignment:Splign:2.1.0"
     exon            5137..7808
                     /gene="Col4a4"
                     /gene_synonym="E130010M05Rik; [a]4(IV)"
                     /inference="alignment:Splign:2.1.0"
     polyA_site      5709
                     /gene="Col4a4"
                     /gene_synonym="E130010M05Rik; [a]4(IV)"
                     /note="major polyA site"
     regulatory      5734..5739
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Col4a4"
                     /gene_synonym="E130010M05Rik; [a]4(IV)"
                     /note="hexamer: TATAAA"
     polyA_site      5768
                     /gene="Col4a4"
                     /gene_synonym="E130010M05Rik; [a]4(IV)"
     regulatory      6590..6595
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Col4a4"
                     /gene_synonym="E130010M05Rik; [a]4(IV)"
                     /note="hexamer: AACAAA"
     polyA_site      6613
                     /gene="Col4a4"
                     /gene_synonym="E130010M05Rik; [a]4(IV)"
ORIGIN      
        1 gaggcgcgcg ggagtctgca actccggagg ccaagtttgc tggcagctct agggactgcc
       61 catcggctgc cggccttcaa ctcgcagcca gcacgcagac ctgaggctgg acaggctggg
      121 gagaggaaca cggtcacggt cacgggtgga ttccctccag ccataacctc gaggagcctg
      181 tctggtctgc ctgctaccct gctgttcctt ctttgcccgg ttgctcccca gattctaaca
      241 ggacactgac tcccggctgt ccatgagagt cttctggaag agaggagaag gctggaggag
      301 tattttgggc acagaggtca cccctcgagg gcaccagtgc tccaggtctt catgaggtgc
      361 tttttcagat ggaccaagtc ctttgtcaca gccccctggt cactaatatt tatcctcttt
      421 actatacaat atgaatatgg gagtggcaag aagtatggcg gcccctgcgg aggaagaaac
      481 tgctctgtct gccagtgttt tcctgagaag ggatctcggg gtcacccagg accactggga
      541 ccacagggac caatagggcc cttgggaccc ctgggaccta ttgggattcc aggagaaaaa
      601 ggagagagag gagacagtgg ctcacctgga ccaccaggag agaaaggaga caagggtcca
      661 actggtgtcc ctggatttcc aggtgtggat ggtgtacctg gtcacccagg gcctcctgga
      721 cccagaggca aacctggtgt ggacggctac aatggctcac gtggtgatcc gggctatcca
      781 ggagaaagag gagctccagg cccaggcggc ccccctggtc agcctgggga aaatggagaa
      841 aaaggaagat ctgtgtacat tactggtggc gttaaaggta ttcagggaga ccgtggggac
      901 ccaggaccac ccggcttacc aggatctagg ggtgcacaag ggtcaccagg gcccatgggg
      961 catgcaggcg caccagggct agcaggacct ataggtcatc ctggaagccc tgggttgaag
     1021 ggaaatcctg ccactggact gaagggacaa aggggagagc cgggtgaagt tggccagcgt
     1081 ggtcctcctg ggcccacgct gttggtacag ccgcctgatt tgagtatcta taaaggagaa
     1141 aagggtgtaa aaggaatgcc tggaatgatt ggtcccccag gacctccagg tcgcaaggga
     1201 gcgcctggtg tcgggatcaa aggagagaaa ggtatccctg ggttcccagg accccggggt
     1261 gagcctggtt cgcatgggcc tccaggtttt ccaggattca aggggataca aggagcagct
     1321 ggagagcctg ggctgttcgg atttcttggt ccaaaggggg atctgggaga tcgcgggtac
     1381 ccgggaccac caggcatttt gttgactcca gctccaccac tcaaaggtgt tccaggggac
     1441 ccaggacccc ctggctacta tggggagatt ggagatgttg gattaccagg tcctccaggg
     1501 cccccaggca gaccagggga aacttgtcca ggcatgatgg gacctcctgg accaccaggg
     1561 gttcctggtc ctccaggatt tccaggagaa gctggggttc ctggaagact cgactgtgcc
     1621 ccaggaaaac ctgggaagcc agggctaccc ggcctgcctg gcgcaccagg accagagggg
     1681 ccccctggat cagatgtgat atactgtcgc cctgggtgcc ctgggccaat gggtgaaaag
     1741 ggaaaagtgg gtcctccagg aagaagagga gccaaaggag caaaaggaaa caagggactc
     1801 tgtacctgtc cacctggtcc catgggaccc cctggtcccc caggacctcc tggaagacaa
     1861 ggaagtaaag gagacttagg gctccctggt tggcatggag aaaagggcga tccaggtcag
     1921 cctggtgctg aaggaccgcc agggccacca ggaagacctg gtgccatggg gccccctggt
     1981 cacaaagggg aaaagggtga catggtcata tcaagagtga aagggcaaaa aggagaaaga
     2041 ggactggatg ggccaccagg atttccgggg ccacatggac aagatggtgg ggatggacgt
     2101 cctggagaaa gaggggatcc tgggccacga ggggaccata aggatgcagc cccgggtgag
     2161 agagggcttc ccggactgcc aggccctcca ggaagaacag gacctgaggg gcctccaggc
     2221 ctgggatttc caggcccacc aggacagcga ggcctacccg gagagcccgg gcgtccgggc
     2281 accagaggct ttgatggcac gaaggggcag aaaggtgact ccattctgtg taatgtaagc
     2341 tacccgggaa aaccaggtct gccaggtctt gatggacctc caggtctgaa ggggtttcca
     2401 ggccccccag gggctcctgg tatgagatgt ccagatgggc agaaaggcca gcggggcaaa
     2461 ccgggaatgt ctgggattcc aggtccacct ggttttcgtg gtgacatggg agatccaggc
     2521 atcaaaggtg aaaaggggac ctcccctatt ggacccccag gcccacctgg gtctcctgga
     2581 aaggatggcc agaaaggaat cccaggagac cctgcatttg gtgacccagg accccctgga
     2641 gagaggggtc ttccaggagc accaggcatg aaaggacaga aaggtcaccc agggtgccca
     2701 ggggctggag gccctcctgg catccctgga tctccaggtc tcaaaggtcc caaaggtaga
     2761 gagggaagtc gtggctttcc agggatccca ggatcccctg gccattcctg tgaaagaggt
     2821 gctcccggga tcccaggaca accgggactt cctggaactc caggtgatcc aggggcccca
     2881 ggttggaaag gccagccagg agacatgggt ccctctggac cagctgggat gaagggcctc
     2941 cctggtctcc caggcctacc aggagctgat ggtctccgag ggcctcctgg gattccaggc
     3001 cccaatgggg aagatggtct acctggtctt ccaggcctga agggactccc agggctgcct
     3061 ggcttccctg gttttccagg agagagagga aagccagggc cagatggaga acctggaaga
     3121 aagggagagg ttggagagaa aggctggcct ggcttgaagg gagacctggg agagcgaggc
     3181 gccaaaggag acagaggact tcctggtgat gcaggagaag cagtcacttc cagaaagggt
     3241 gaacctgggg atgctgggcc tccaggagat ggtgggttct caggagagag aggtgataaa
     3301 ggaagctcag ggatgcgagg gggaagaggg gatcccggaa gagatggact accgggactc
     3361 cacagagggc agcctgggat agatgggcct cctgggcctc ctgggccccc tggccctcca
     3421 ggatcacctg gactgagagg agtcattggt ttcccaggat ttccaggtga ccagggtgat
     3481 ccaggttctc caggaccccc tggattccca ggagatgatg gagcaagagg acctaaagga
     3541 tacaaaggtg accctgccag tcagtgtggt ccacctggtc caaagggtga gccaggtagt
     3601 cctggatacc aaggacgtac tggagtcccc ggagagaaag gctttcctgg agatgaagga
     3661 ccccgaggac caccaggcag acctggacag cctggatctt ttggaccacc agggtgtcca
     3721 ggtgacccag ggatgcccgg actcaaggga catccaggag aagtgggaga ccctgggcca
     3781 agaggtgatg cgggagattt cgggaggcca ggtcctgctg gagtgaaagg gcctctcggg
     3841 tctcctggtc tgaatggctt acatggtctg aagggtgaga aaggaacaaa aggcgcctca
     3901 ggtttgctcg aaatgggtcc acctgggcca atggggatgc ctgggcaaaa aggagagaaa
     3961 ggagaccctg ggagtccagg gatttctcct ccaggtcttc ctggagaaaa gggcttccca
     4021 ggacccccag ggagaccagg accacctggt cccgcaggtg ccccaggaag agctgctaaa
     4081 ggtgacattc ctgatccagg tccacctgga gaccggggac ctcctggccc cgatggccca
     4141 agaggagtac ctgggcctcc gggttcccct gggaatgttg accttctgaa aggggaccca
     4201 ggtgactgtg gtttgccagg accacctggc tcccgaggcc caccaggccc tccagggtgt
     4261 cagggtcccc caggatgtga tggcaaagat ggccagaaag gaccaatggg acttccaggg
     4321 ttgccagggc cacctggtct tcctggggca cctggcgaga agggattacc tggccctcca
     4381 ggcagaaaag ggccagtagg tcctccaggc tgcagaggtg aacctgggcc tcctgcagac
     4441 gtggattcct gtcctcgaat cccaggactt cctggagtgc caggtcccag aggaccagaa
     4501 ggagctatgg gggaacctgg acggagaggc ctgcctggac cagggtgcaa aggagagcct
     4561 ggaccggatg gcagacgggg ccaggatggc atccctggat ctcccgggcc tcctggacgc
     4621 aaaggtgaca ctggagaggc agggtgtcct ggagcaccag gccctcctgg tccaactggt
     4681 gatcctgggc ccaaggggtt tggccctgga tcgctcagtg gcttcctcct ggttctccac
     4741 agtcagactg accaggaacc ggcctgcccc gtgggcatgc ctcggctttg gacagggtac
     4801 agcttgttgt acatggaagg acaggagaag gcgcacaatc aagatcttgg tttggcaggc
     4861 tcttgtcttc ctgtgtttag cactctgccc tttgcctact gcaacatcca ccaagtgtgc
     4921 cactacgccc agagaaatga caggtcctac tggctgtcca gtgcggctcc tcttcctatg
     4981 atgccactct cagaggaaga gatccgctct tacatcagcc gctgtgcggt gtgtgaggcc
     5041 ccagcacagg ccgtggcggt ccacagtcag gaccaatcca tcccaccgtg tccacggaca
     5101 tggaggagtc tctggattgg gtactcattt ctgatgcaca ctggagctgg ggaccaagga
     5161 ggtgggcagg cactgatgtc acctggcagc tgcctagagg acttcagagc tgcaccgttc
     5221 gttgaatgcc agggccgaca gggaacctgt cacttttttg caaacgagta cagcttctgg
     5281 ctgaccaccg tgaacccaga cctgcagttt gcctctggcc cgtcaccaga caccttgaaa
     5341 gaagttcagg cccagcgccg gaaaatcagc aggtgccagg tttgcatgaa gcacagctaa
     5401 agaattcaaa acatgccaac atgccaaccc gcatcaaggc agacatccag tggaagacaa
     5461 acttcctcaa ctattccagc aaattcagtg gtgctctctc cagacttctg ccatgccttg
     5521 ttctcagcgt ggttattccc ggcctctcct cctcctcctc ctcctcctcc tcctcctcct
     5581 cctcctcctc ctcctcctcc tcctctatgg atcaagcaaa cactgtacat gtggagttgc
     5641 tcccccttct ccttttattg agattataga gaacagactt tatttgaaca tgtgttaatt
     5701 cccaaaaaag caagcggatg acaaagacca gattataaac ttcattcatt ggtcgataga
     5761 gtctcaaaca actgaagtca attattctgt aatctattgg gcttctggct agattttata
     5821 ggaaaaaaaa tataaatagg ccaacaaagg aagcgatgaa gtagagattt ttcagtgttt
     5881 caaaatgatt ccctctataa tctttttttt tttttaagcc aggcacttta aataattgtg
     5941 agaccatggc ccaaagaggt ttttaaaaag gaggaagaaa aagaaaacca acctttctac
     6001 actgccagca ttagcagatc atttcaaagc agaaatgggt cattatgcag gaaggcctgc
     6061 agtcctgtat tccagagcct cgagattagc ataaacactt cacagatgaa tataaaacat
     6121 tatgttctct tctgcatttt acagagaatg gaaatgccta ctttggcaac ccctttgaaa
     6181 agtagcaatt atagaagaaa tatattcaat aagggattag gggcctaaaa gctattaatg
     6241 aatattaagg tagtgattca caaaaattga ctcccccgtc ccagggaact cgcaaacaga
     6301 ccacttttcc ccagtcgggg gccagcatgt cctcctgcta atgggactga agccacatgg
     6361 ggcttgctca ggcaagggag agcctgatgc aaagccactg gctttccttc ccaggagaaa
     6421 ctgtgaggct cctcctccag atggataatg gtgactcact ccggaagtga cagggctatc
     6481 attatgcact tgggagaaaa ttaagggctg acttaattaa acttaggtaa gaagattcgt
     6541 ttatgtcagg gtcaccccac caggaaagca tggggctctc ttgaacagaa acaaagacta
     6601 tagccaatct gaattttgtg tccctgtgat tttttttttt taaattggag agcatctgtc
     6661 tctccaattt tcaaacattc tttatattgt attagcatca tggcaagaca gttgtttgac
     6721 actggtactt agcagctcct gtacacgggt aggatctctg tcaatttgca gtgacttttc
     6781 tgcataccaa aaggtctgct tgctatcaag ttggggatgc ctacaaacca caaattgaaa
     6841 ggtttgctag gaaagatttt ttattttttt ttgaaactca ccttcctcag ccaaagtaaa
     6901 attgccattt taatggaaag gaaggatttt ttccccactg agagagacac tcatgtcaga
     6961 gctcactttt cagtgcactt tagaataatt gaaagaaaaa ctcaattaaa atgtggtttc
     7021 cgaggctggg gggcaggggg gcgactcggg ctaacagcat ttactgcttt ttcagaggag
     7081 gatctgaatt cagttcctgg cacccaggaa ctggctccca gtcaccagga actccagtcc
     7141 ccctttctgg ctcctagggg cccttctgta cccatgcaaa cgcccaccca cagacatact
     7201 catacacata gaaacaaaag taaataatga tacttctaaa gagtgtttgc cttgccatca
     7261 tttggccaat gatgaattcc ttcatgcaga atacatttag catttttatc tctgtaggat
     7321 gtctatggga tggcatttta agataagttt ctggtaaata acattttatt ttattatgtt
     7381 ttaagatagc accccagtct atagttcaag ttggcctgaa acttgctaca caacctcaca
     7441 ttggcttcaa tatcctggca attgcctggc gtcccagaag tgttagaagc ccagccttta
     7501 aaaaaaaaaa aaaaaaaaca cggtttttgt tttgctttga agacgttgtc tctgcttggt
     7561 ctggaacttg ctatataacc agggccaaac atctctattt taacataaga tattttctcg
     7621 atgtaacaga agtttttcta ttgacccatt tgattctaag ttcaatgctg tgcatgttaa
     7681 atattttttg agtgtccatt tctcctttat ttacattact cttacgtttc cagattctac
     7741 atttatcatt ctaaaccagg cctgaacttg gcctgagaac atgttcccgg aagaggtggt
     7801 tgggaata
//
