LOCUS       NP_031761               1682 aa            linear   ROD 18-MAR-2025
DEFINITION  collagen alpha-4(IV) chain precursor [Mus musculus].
ACCESSION   NP_031761 NP_795989
VERSION     NP_031761.1
DBSOURCE    REFSEQ: accession NM_007735.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (residues 1 to 1682)
  AUTHORS   Wang,M., Liu,H., Zhang,M., Niu,X., Sun,M., Wang,F., Ni,Y., Hong,T.,
            Zhang,W. and Dang,S.
  TITLE     The secreted protease ADAMTS18 links early isoform transformation
            and maturation of glomerular basement membrane macromolecules to
            the integrity of the glomerular filtration barrier
  JOURNAL   Biochem Biophys Res Commun 750, 151386 (2025)
   PUBMED   39879696
REFERENCE   2  (residues 1 to 1682)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (residues 1 to 1682)
  AUTHORS   Lipp,S.N., Jacobson,K.R., Schwaderer,A.L., Hains,D.S. and Calve,S.
  TITLE     FOXD1 is required for 3D patterning of the kidney interstitial
            matrix
  JOURNAL   Dev Dyn 252 (4), 463-482 (2023)
   PUBMED   36335435
REFERENCE   4  (residues 1 to 1682)
  AUTHORS   Aypek,H., Krisp,C., Lu,S., Liu,S., Kylies,D., Kretz,O., Wu,G.,
            Moritz,M., Amann,K., Benz,K., Tong,P., Hu,Z.M., Alsulaiman,S.M.,
            Khan,A.O., Grohmann,M., Wagner,T., Muller-Deile,J., Schluter,H.,
            Puelles,V.G., Bergmann,C., Huber,T.B. and Grahammer,F.
  TITLE     Loss of the collagen IV modifier prolyl 3-hydroxylase 2 causes thin
            basement membrane nephropathy
  JOURNAL   J Clin Invest 132 (9) (2022)
   PUBMED   35499085
REFERENCE   5  (residues 1 to 1682)
  AUTHORS   Damen,M., Wirtz,L., Soroka,E., Khatif,H., Kukat,C., Simons,B.D. and
            Bazzi,H.
  TITLE     High proliferation and delamination during skin epidermal
            stratification
  JOURNAL   Nat Commun 12 (1), 3227 (2021)
   PUBMED   34050161
  REMARK    Publication Status: Online-Only
REFERENCE   6  (residues 1 to 1682)
  AUTHORS   Thorner,P.S., Zheng,K., Kalluri,R., Jacobs,R. and Hudson,B.G.
  TITLE     Coordinate gene expression of the alpha3, alpha4, and alpha5 chains
            of collagen type IV. Evidence from a canine model of X-linked
            nephritis with a COL4A5 gene mutation
  JOURNAL   J Biol Chem 271 (23), 13821-13828 (1996)
   PUBMED   8662866
REFERENCE   7  (residues 1 to 1682)
  AUTHORS   Enders,G.C., Kahsai,T.Z., Lian,G., Funabiki,K., Killen,P.D. and
            Hudson,B.G.
  TITLE     Developmental changes in seminiferous tubule extracellular matrix
            components of the mouse testis: alpha 3(IV) collagen chain
            expressed at the initiation of spermatogenesis
  JOURNAL   Biol Reprod 53 (6), 1489-1499 (1995)
   PUBMED   8562707
REFERENCE   8  (residues 1 to 1682)
  AUTHORS   Miner,J.H. and Sanes,J.R.
  TITLE     Collagen IV alpha 3, alpha 4, and alpha 5 chains in rodent basal
            laminae: sequence, distribution, association with laminins, and
            developmental switches
  JOURNAL   J Cell Biol 127 (3), 879-891 (1994)
   PUBMED   7962065
REFERENCE   9  (residues 1 to 1682)
  AUTHORS   Wu,J.E. and Santoro,S.A.
  TITLE     Complex patterns of expression suggest extensive roles for the
            alpha 2 beta 1 integrin in murine development
  JOURNAL   Dev Dyn 199 (4), 292-314 (1994)
   PUBMED   7521231
REFERENCE   10 (residues 1 to 1682)
  AUTHORS   Saga,Y., Yagi,T., Ikawa,Y., Sakakura,T. and Aizawa,S.
  TITLE     Mice develop normally without tenascin
  JOURNAL   Genes Dev 6 (10), 1821-1831 (1992)
   PUBMED   1383086
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK164732.1, AF169388.1 and AC160543.13.
            
            On Dec 17, 2005 this sequence version replaced NP_795989.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF169388.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..1682
                     /organism="Mus musculus"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="1"
                     /map="1 42.2 cM"
     Protein         1..1682
                     /product="collagen alpha-4(IV) chain precursor"
                     /note="collagen alpha-4(IV) chain; procollagen, type IV,
                     alpha 4"
                     /calculated_mol_wt=160219
     sig_peptide     1..32
                     /inference="COORDINATES: ab initio prediction:SignalP:6.0"
                     /calculated_mol_wt=3897
     Region          31..56
                     /region_name="7S domain.
                     /evidence=ECO:0000250|UniProtKB:P53420"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9QZR9.1)"
     mat_peptide     33..1682
                     /product="Collagen alpha-4(IV) chain.
                     /evidence=ECO:0000255. /id=PRO_0000283793"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9QZR9.1)"
                     /calculated_mol_wt=160219
     Site            43
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (Q9QZR9.1)"
     Region          <54..>356
                     /region_name="gly_rich_SclB"
                     /note="LPXTG-anchored collagen-like adhesin Scl2/SclB;
                     NF038329"
                     /db_xref="CDD:468478"
     Region          56..255
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9QZR9.1)"
     Region          57..1451
                     /region_name="Triple-helical region.
                     /evidence=ECO:0000250|UniProtKB:P53420"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9QZR9.1)"
     Region          86..88
                     /region_name="Cell attachment site. /evidence=ECO:0000255"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9QZR9.1)"
     Site            134
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (Q9QZR9.1)"
     Region          137..139
                     /region_name="Cell attachment site. /evidence=ECO:0000255"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9QZR9.1)"
     Region          181..183
                     /region_name="Cell attachment site. /evidence=ECO:0000255"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9QZR9.1)"
     Region          <288..>483
                     /region_name="gly_rich_SclB"
                     /note="LPXTG-anchored collagen-like adhesin Scl2/SclB;
                     NF038329"
                     /db_xref="CDD:468478"
     Region          379..1453
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9QZR9.1)"
     Region          <495..>727
                     /region_name="gly_rich_SclB"
                     /note="LPXTG-anchored collagen-like adhesin Scl2/SclB;
                     NF038329"
                     /db_xref="CDD:468478"
     Region          587..589
                     /region_name="Cell attachment site. /evidence=ECO:0000255"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9QZR9.1)"
     Region          593..595
                     /region_name="Cell attachment site. /evidence=ECO:0000255"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9QZR9.1)"
     Site            661
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (Q9QZR9.1)"
     Region          <695..>924
                     /region_name="gly_rich_SclB"
                     /note="LPXTG-anchored collagen-like adhesin Scl2/SclB;
                     NF038329"
                     /db_xref="CDD:468478"
     Region          716..718
                     /region_name="Cell attachment site. /evidence=ECO:0000255"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9QZR9.1)"
     Region          <908..>1217
                     /region_name="gly_rich_SclB"
                     /note="LPXTG-anchored collagen-like adhesin Scl2/SclB;
                     NF038329"
                     /db_xref="CDD:468478"
     Region          980..982
                     /region_name="Cell attachment site. /evidence=ECO:0000255"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9QZR9.1)"
     Region          992..994
                     /region_name="Cell attachment site. /evidence=ECO:0000255"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9QZR9.1)"
     Region          <1142..>1412
                     /region_name="gly_rich_SclB"
                     /note="LPXTG-anchored collagen-like adhesin Scl2/SclB;
                     NF038329"
                     /db_xref="CDD:468478"
     Region          1144..1146
                     /region_name="Cell attachment site. /evidence=ECO:0000255"
                     /note="propagated from UniProtKB/Swiss-Prot (Q9QZR9.1)"
     Site            1197..1198
                     /site_type="cleavage"
                     /note="Cleavage, by collagenase. /evidence=ECO:0000250;
                     propagated from UniProtKB/Swiss-Prot (Q9QZR9.1)"
     Region          1458..1563
                     /region_name="C4"
                     /note="C-terminal tandem repeated domain in type 4
                     procollagen; pfam01413"
                     /db_xref="CDD:460201"
     Region          1566..1680
                     /region_name="C4"
                     /note="C-terminal tandem repeated domain in type 4
                     procollagen; pfam01413"
                     /db_xref="CDD:460201"
     CDS             1..1682
                     /gene="Col4a4"
                     /gene_synonym="E130010M05Rik; [a]4(IV)"
                     /coded_by="NM_007735.2:352..5400"
                     /db_xref="CCDS:CCDS35630.1"
                     /db_xref="GeneID:12829"
                     /db_xref="MGI:MGI:104687"
ORIGIN      
        1 mrcffrwtks fvtapwslif ilftiqyeyg sgkkyggpcg grncsvcqcf pekgsrghpg
       61 plgpqgpigp lgplgpigip gekgergdsg spgppgekgd kgptgvpgfp gvdgvpghpg
      121 ppgprgkpgv dgyngsrgdp gypgergapg pggppgqpge ngekgrsvyi tggvkgiqgd
      181 rgdpgppglp gsrgaqgspg pmghagapgl agpighpgsp glkgnpatgl kgqrgepgev
      241 gqrgppgptl lvqppdlsiy kgekgvkgmp gmigppgppg rkgapgvgik gekgipgfpg
      301 prgepgshgp pgfpgfkgiq gaagepglfg flgpkgdlgd rgypgppgil ltpapplkgv
      361 pgdpgppgyy geigdvglpg ppgppgrpge tcpgmmgppg ppgvpgppgf pgeagvpgrl
      421 dcapgkpgkp glpglpgapg pegppgsdvi ycrpgcpgpm gekgkvgppg rrgakgakgn
      481 kglctcppgp mgppgppgpp grqgskgdlg lpgwhgekgd pgqpgaegpp gppgrpgamg
      541 ppghkgekgd mvisrvkgqk gergldgppg fpgphgqdgg dgrpgergdp gprgdhkdaa
      601 pgerglpglp gppgrtgpeg ppglgfpgpp gqrglpgepg rpgtrgfdgt kgqkgdsilc
      661 nvsypgkpgl pgldgppglk gfpgppgapg mrcpdgqkgq rgkpgmsgip gppgfrgdmg
      721 dpgikgekgt spigppgppg spgkdgqkgi pgdpafgdpg ppgerglpga pgmkgqkghp
      781 gcpgaggppg ipgspglkgp kgregsrgfp gipgspghsc ergapgipgq pglpgtpgdp
      841 gapgwkgqpg dmgpsgpagm kglpglpglp gadglrgppg ipgpngedgl pglpglkglp
      901 glpgfpgfpg ergkpgpdge pgrkgevgek gwpglkgdlg ergakgdrgl pgdageavts
      961 rkgepgdagp pgdggfsger gdkgssgmrg grgdpgrdgl pglhrgqpgi dgppgppgpp
     1021 gppgspglrg vigfpgfpgd qgdpgspgpp gfpgddgarg pkgykgdpas qcgppgpkge
     1081 pgspgyqgrt gvpgekgfpg degprgppgr pgqpgsfgpp gcpgdpgmpg lkghpgevgd
     1141 pgprgdagdf grpgpagvkg plgspglngl hglkgekgtk gasgllemgp pgpmgmpgqk
     1201 gekgdpgspg isppglpgek gfpgppgrpg ppgpagapgr aakgdipdpg ppgdrgppgp
     1261 dgprgvpgpp gspgnvdllk gdpgdcglpg ppgsrgppgp pgcqgppgcd gkdgqkgpmg
     1321 lpglpgppgl pgapgekglp gppgrkgpvg ppgcrgepgp padvdscpri pglpgvpgpr
     1381 gpegamgepg rrglpgpgck gepgpdgrrg qdgipgspgp pgrkgdtgea gcpgapgppg
     1441 ptgdpgpkgf gpgslsgfll vlhsqtdqep acpvgmprlw tgysllymeg qekahnqdlg
     1501 lagsclpvfs tlpfaycnih qvchyaqrnd rsywlssaap lpmmplseee irsyisrcav
     1561 ceapaqavav hsqdqsippc prtwrslwig ysflmhtgag dqgggqalms pgscledfra
     1621 apfvecqgrq gtchffaney sfwlttvnpd lqfasgpspd tlkevqaqrr kisrcqvcmk
     1681 hs
//
