LOCUS       NM_009931               6636 bp    mRNA    linear   ROD 18-MAR-2025
DEFINITION  Mus musculus collagen, type IV, alpha 1 (Col4a1), transcript
            variant 1, mRNA.
ACCESSION   NM_009931
VERSION     NM_009931.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 6636)
  AUTHORS   Wang,M., Liu,H., Zhang,M., Niu,X., Sun,M., Wang,F., Ni,Y., Hong,T.,
            Zhang,W. and Dang,S.
  TITLE     The secreted protease ADAMTS18 links early isoform transformation
            and maturation of glomerular basement membrane macromolecules to
            the integrity of the glomerular filtration barrier
  JOURNAL   Biochem Biophys Res Commun 750, 151386 (2025)
   PUBMED   39879696
REFERENCE   2  (bases 1 to 6636)
  AUTHORS   McNeilly,S., Thomson,C.R., Gonzalez-Trueba,L., Sin,Y.Y.,
            Granata,A., Hamilton,G., Lee,M., Boland,E., McClure,J.D.,
            Lumbreras-Perales,C., Aman,A., Kumar,A.A., Cantini,M., Gok,C.,
            Graham,D., Tomono,Y., Anderson,C.D., Lu,Y., Smith,C., Markus,H.S.,
            Abramowicz,M., Vilain,C., Al-Shahi Salman,R., Salmeron-Sanchez,M.,
            Hainsworth,A.H., Fuller,W., Kadler,K.E., Bulleid,N.J. and Van
            Agtmael,T.
  TITLE     Collagen IV deficiency causes hypertrophic remodeling and
            endothelium-dependent hyperpolarization in small vessel disease
            with intracerebral hemorrhage
  JOURNAL   EBioMedicine 107, 105315 (2024)
   PUBMED   39216230
  REMARK    GeneRIF: Collagen IV deficiency causes hypertrophic remodeling and
            endothelium-dependent hyperpolarization in small vessel disease
            with intracerebral hemorrhage.
REFERENCE   3  (bases 1 to 6636)
  AUTHORS   Patel,V.N., Aure,M.H., Choi,S.H., Ball,J.R., Lane,E.D., Wang,Z.,
            Xu,Y., Zheng,C., Liu,X., Martin,D., Pailin,J.Y., Prochazkova,M.,
            Kulkarni,A.B., van Kuppevelt,T.H., Ambudkar,I.S., Liu,J. and
            Hoffman,M.P.
  TITLE     Specific 3-O-sulfated heparan sulfate domains regulate salivary
            gland basement membrane metabolism and epithelial differentiation
  JOURNAL   Nat Commun 15 (1), 7584 (2024)
   PUBMED   39217171
  REMARK    Erratum:[Nat Commun. 2024 Oct 10;15(1):8784. doi:
            10.1038/s41467-024-53230-4. PMID: 39389981]
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 6636)
  AUTHORS   Despin-Guitard,E., Rosa,V.S., Plunder,S., Mathiah,N., Van
            Schoor,K., Nehme,E., Merino-Aceituno,S., Egea,J., Shahbazi,M.N.,
            Theveneau,E. and Migeotte,I.
  TITLE     Non-apical mitoses contribute to cell delamination during mouse
            gastrulation
  JOURNAL   Nat Commun 15 (1), 7364 (2024)
   PUBMED   39198421
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 6636)
  AUTHORS   Wang,L., He,L., Yi,W., Wang,M., Xu,F., Liu,H., Nie,J., Pan,Y.H.,
            Dang,S. and Zhang,W.
  TITLE     ADAMTS18-fibronectin interaction regulates the morphology of liver
            sinusoidal endothelial cells
  JOURNAL   iScience 27 (7), 110273 (2024)
   PUBMED   39040056
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 6636)
  AUTHORS   Saga,Y., Yagi,T., Ikawa,Y., Sakakura,T. and Aizawa,S.
  TITLE     Mice develop normally without tenascin
  JOURNAL   Genes Dev 6 (10), 1821-1831 (1992)
   PUBMED   1383086
REFERENCE   7  (bases 1 to 6636)
  AUTHORS   Bruggeman,L.A., Burbelo,P.D., Yamada,Y. and Klotman,P.E.
  TITLE     A novel sequence in the type IV collagen promoter binds nuclear
            proteins from Engelbreth-Holm-Swarm tumor
  JOURNAL   Oncogene 7 (8), 1497-1502 (1992)
   PUBMED   1630813
REFERENCE   8  (bases 1 to 6636)
  AUTHORS   Killen,P.D., Burbelo,P.D., Martin,G.R. and Yamada,Y.
  TITLE     Characterization of the promoter for the alpha 1 (IV) collagen
            gene. DNA sequences within the first intron enhance transcription
  JOURNAL   J Biol Chem 263 (25), 12310-12314 (1988)
   PUBMED   2842328
REFERENCE   9  (bases 1 to 6636)
  AUTHORS   Oberbaumer,I.
  TITLE     New pUC-derived expression vectors for rapid construction of cDNA
            libraries
  JOURNAL   Gene 49 (1), 81-91 (1986)
   PUBMED   2436975
REFERENCE   10 (bases 1 to 6636)
  AUTHORS   Oberbaumer,I., Laurent,M., Schwarz,U., Sakurai,Y., Yamada,Y.,
            Vogeli,G., Voss,T., Siebold,B., Glanville,R.W. and Kuhn,K.
  TITLE     Amino acid sequence of the non-collagenous globular domain (NC1) of
            the alpha 1(IV) chain of basement membrane collagen as derived from
            complementary DNA
  JOURNAL   Eur J Biochem 147 (2), 217-224 (1985)
   PUBMED   2578961
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC114598.9, CJ108618.1,
            AK147661.1, AC115800.13 and CJ248638.1.
            
            On Dec 1, 2007 this sequence version replaced NM_009931.1.
            
            Summary: This gene encodes the alpha-1 subunit of the type IV
            collagens, an essential component of basement membranes. The
            encoded protein forms a triple helical heterotrimer comprised of
            two alpha-1 and one alpha-2 subunits that assembles into a type IV
            collagen network. This gene is located adjacent to the gene
            encoding alpha-2 subunit. Mice lacking both the alpha-1 and alpha-2
            subunits of collagen IV die in utero due to structural deficiencies
            in the basement membranes and certain mutations in this gene cause
            perinatal cerebral hemorrhage and porencephaly. Alternative
            splicing of this gene results in multiple transcript variants.
            [provided by RefSeq, Nov 2015].
            
            Transcript Variant: This variant (1) represents the shorter
            transcript and encodes the functional protein.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK147661.1, AK147284.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849381
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2                 AC114598.9         122941-122942
            3-41                CJ108618.1         2-40
            42-5026             AK147661.1         8-4992
            5027-5027           AC115800.13        164258-164258       c
            5028-6607           AK147661.1         4994-6573
            6608-6636           CJ248638.1         431-459
FEATURES             Location/Qualifiers
     source          1..6636
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="8"
                     /map="8 5.53 cM"
     gene            1..6636
                     /gene="Col4a1"
                     /gene_synonym="Bru; Col4a-1; Raw; Svc"
                     /note="collagen, type IV, alpha 1"
                     /db_xref="GeneID:12826"
                     /db_xref="MGI:MGI:88454"
     exon            1..311
                     /gene="Col4a1"
                     /gene_synonym="Bru; Col4a-1; Raw; Svc"
                     /inference="alignment:Splign:2.1.0"
     CDS             228..5237
                     /gene="Col4a1"
                     /gene_synonym="Bru; Col4a-1; Raw; Svc"
                     /note="collagen alpha-1(IV) chain; alpha1(IV) collagen;
                     procollagen, type IV, alpha 1; alpha-1 type IV collagen"
                     /codon_start=1
                     /product="collagen alpha-1(IV) chain precursor"
                     /protein_id="NP_034061.2"
                     /db_xref="CCDS:CCDS40219.1"
                     /db_xref="GeneID:12826"
                     /db_xref="MGI:MGI:88454"
                     /translation="MGPRLSVWLLLLFAALLLHEERSRAAAKGDCGGSGCGKCDCHGV
                     KGQKGERGLPGLQGVIGFPGMQGPEGPHGPPGQKGDAGEPGLPGTKGTRGPPGAAGYP
                     GNPGLPGIPGQDGPPGPPGIPGCNGTKGERGPLGPPGLPGFSGNPGPPGLPGMKGDPG
                     EILGHVPGTLLKGERGFPGIPGMPGSPGLPGLQGPVGPPGFTGPPGPPGPPGPPGEKG
                     QMGSSFQGPKGDKGEQGVSGPPGVPGQAQVKEKGDFAPTGEKGQKGEPGFPGVPGYGE
                     KGEPGKQGPRGKPGKDGEKGERGSPGIPGDSGYPGLPGRQGPQGEKGEAGLPGPPGTV
                     IGTMPLGEKGDRGYPGAPGLRGEPGPKGFPGTPGQPGPPGFPTPGQAGAPGFPGERGE
                     KGDQGFPGVSLPGPSGRDGAPGPPGPPGPPGQPGHTNGIVECQPGPPGDQGPPGTPGQ
                     PGLTGEVGQKGQKGESCLACDTEGLRGPPGPQGPPGEIGFPGQPGAKGDRGLPGRDGL
                     EGLPGPQGSPGLIGQPGAKGEPGEIFFDMRLKGDKGDPGFPGQPGMPGRAGTPGRDGH
                     PGLPGPKGSPGSIGLKGERGPPGGVGFPGSRGDIGPPGPPGVGPIGPVGEKGQAGFPG
                     GPGSPGLPGPKGEAGKVVPLPGPPGAAGLPGSPGFPGPQGDRGFPGTPGRPGIPGEKG
                     AVGQPGIGFPGLPGPKGVDGLPGEIGRPGSPGRPGFNGLPGNPGPQGQKGEPGIGLPG
                     LKGQPGLPGIPGTPGEKGSIGGPGVPGEQGLTGPPGLQGIRGDPGPPGVQGPAGPPGV
                     PGIGPPGAMGPPGGQGPPGSSGPPGIKGEKGFPGFPGLDMPGPKGDKGSQGLPGLTGQ
                     SGLPGLPGQQGTPGVPGFPGSKGEMGVMGTPGQPGSPGPAGTPGLPGEKGDHGLPGSS
                     GPRGDPGFKGDKGDVGLPGMPGSMEHVDMGSMKGQKGDQGEKGQIGPTGDKGSRGDPG
                     TPGVPGKDGQAGHPGQPGPKGDPGLSGTPGSPGLPGPKGSVGGMGLPGSPGEKGVPGI
                     PGSQGVPGSPGEKGAKGEKGQSGLPGIGIPGRPGDKGDQGLAGFPGSPGEKGEKGSAG
                     TPGMPGSPGPRGSPGNIGHPGSPGLPGEKGDKGLPGLDGVPGVKGEAGLPGTPGPTGP
                     AGQKGEPGSDGIPGSAGEKGEQGVPGRGFPGFPGSKGDKGSKGEVGFPGLAGSPGIPG
                     VKGEQGFMGPPGPQGQPGLPGTPGHPVEGPKGDRGPQGQPGLPGHPGPMGPPGFPGIN
                     GPKGDKGNQGWPGAPGVPGPKGDPGFQGMPGIGGSPGITGSKGDMGLPGVPGFQGQKG
                     LPGLQGVKGDQGDQGVPGPKGLQGPPGPPGPYDVIKGEPGLPGPEGPPGLKGLQGPPG
                     PKGQQGVTGSVGLPGPPGVPGFDGAPGQKGETGPFGPPGPRGFPGPPGPDGLPGSMGP
                     PGTPSVDHGFLVTRHSQTTDDPLCPPGTKILYHGYSLLYVQGNERAHGQDLGTAGSCL
                     RKFSTMPFLFCNINNVCNFASRNDYSYWLSTPEPMPMSMAPISGDNIRPFISRCAVCE
                     APAMVMAVHSQTIQIPQCPNGWSSLWIGYSFVMHTSAGAEGSGQALASPGSCLEEFRS
                     APFIECHGRGTCNYYANAYSFWLATIERSEMFKKPTPSTLKAGELRTHVSRCQVCMRR
                     T"
     exon            312..371
                     /gene="Col4a1"
                     /gene_synonym="Bru; Col4a-1; Raw; Svc"
                     /inference="alignment:Splign:2.1.0"
     exon            372..461
                     /gene="Col4a1"
                     /gene_synonym="Bru; Col4a-1; Raw; Svc"
                     /inference="alignment:Splign:2.1.0"
     exon            462..506
                     /gene="Col4a1"
                     /gene_synonym="Bru; Col4a-1; Raw; Svc"
                     /inference="alignment:Splign:2.1.0"
     exon            507..551
                     /gene="Col4a1"
                     /gene_synonym="Bru; Col4a-1; Raw; Svc"
                     /inference="alignment:Splign:2.1.0"
     exon            552..614
                     /gene="Col4a1"
                     /gene_synonym="Bru; Col4a-1; Raw; Svc"
                     /inference="alignment:Splign:2.1.0"
     exon            615..668
                     /gene="Col4a1"
                     /gene_synonym="Bru; Col4a-1; Raw; Svc"
                     /inference="alignment:Splign:2.1.0"
     exon            669..695
                     /gene="Col4a1"
                     /gene_synonym="Bru; Col4a-1; Raw; Svc"
                     /inference="alignment:Splign:2.1.0"
     exon            696..779
                     /gene="Col4a1"
                     /gene_synonym="Bru; Col4a-1; Raw; Svc"
                     /inference="alignment:Splign:2.1.0"
     exon            780..842
                     /gene="Col4a1"
                     /gene_synonym="Bru; Col4a-1; Raw; Svc"
                     /inference="alignment:Splign:2.1.0"
     exon            843..878
                     /gene="Col4a1"
                     /gene_synonym="Bru; Col4a-1; Raw; Svc"
                     /inference="alignment:Splign:2.1.0"
     exon            879..920
                     /gene="Col4a1"
                     /gene_synonym="Bru; Col4a-1; Raw; Svc"
                     /inference="alignment:Splign:2.1.0"
     exon            921..1007
                     /gene="Col4a1"
                     /gene_synonym="Bru; Col4a-1; Raw; Svc"
                     /inference="alignment:Splign:2.1.0"
     exon            1008..1034
                     /gene="Col4a1"
                     /gene_synonym="Bru; Col4a-1; Raw; Svc"
                     /inference="alignment:Splign:2.1.0"
     exon            1035..1085
                     /gene="Col4a1"
                     /gene_synonym="Bru; Col4a-1; Raw; Svc"
                     /inference="alignment:Splign:2.1.0"
     exon            1086..1130
                     /gene="Col4a1"
                     /gene_synonym="Bru; Col4a-1; Raw; Svc"
                     /inference="alignment:Splign:2.1.0"
     exon            1131..1184
                     /gene="Col4a1"
                     /gene_synonym="Bru; Col4a-1; Raw; Svc"
                     /inference="alignment:Splign:2.1.0"
     exon            1185..1226
                     /gene="Col4a1"
                     /gene_synonym="Bru; Col4a-1; Raw; Svc"
                     /inference="alignment:Splign:2.1.0"
     exon            1227..1311
                     /gene="Col4a1"
                     /gene_synonym="Bru; Col4a-1; Raw; Svc"
                     /inference="alignment:Splign:2.1.0"
     exon            1312..1347
                     /gene="Col4a1"
                     /gene_synonym="Bru; Col4a-1; Raw; Svc"
                     /inference="alignment:Splign:2.1.0"
     exon            1348..1512
                     /gene="Col4a1"
                     /gene_synonym="Bru; Col4a-1; Raw; Svc"
                     /inference="alignment:Splign:2.1.0"
     exon            1513..1608
                     /gene="Col4a1"
                     /gene_synonym="Bru; Col4a-1; Raw; Svc"
                     /inference="alignment:Splign:2.1.0"
     exon            1609..1692
                     /gene="Col4a1"
                     /gene_synonym="Bru; Col4a-1; Raw; Svc"
                     /inference="alignment:Splign:2.1.0"
     exon            1693..1763
                     /gene="Col4a1"
                     /gene_synonym="Bru; Col4a-1; Raw; Svc"
                     /inference="alignment:Splign:2.1.0"
     exon            1764..1955
                     /gene="Col4a1"
                     /gene_synonym="Bru; Col4a-1; Raw; Svc"
                     /inference="alignment:Splign:2.1.0"
     exon            1956..2124
                     /gene="Col4a1"
                     /gene_synonym="Bru; Col4a-1; Raw; Svc"
                     /inference="alignment:Splign:2.1.0"
     exon            2125..2217
                     /gene="Col4a1"
                     /gene_synonym="Bru; Col4a-1; Raw; Svc"
                     /inference="alignment:Splign:2.1.0"
     exon            2218..2322
                     /gene="Col4a1"
                     /gene_synonym="Bru; Col4a-1; Raw; Svc"
                     /inference="alignment:Splign:2.1.0"
     exon            2323..2420
                     /gene="Col4a1"
                     /gene_synonym="Bru; Col4a-1; Raw; Svc"
                     /inference="alignment:Splign:2.1.0"
     exon            2421..2571
                     /gene="Col4a1"
                     /gene_synonym="Bru; Col4a-1; Raw; Svc"
                     /inference="alignment:Splign:2.1.0"
     exon            2572..2685
                     /gene="Col4a1"
                     /gene_synonym="Bru; Col4a-1; Raw; Svc"
                     /inference="alignment:Splign:2.1.0"
     exon            2686..2853
                     /gene="Col4a1"
                     /gene_synonym="Bru; Col4a-1; Raw; Svc"
                     /inference="alignment:Splign:2.1.0"
     exon            2854..2943
                     /gene="Col4a1"
                     /gene_synonym="Bru; Col4a-1; Raw; Svc"
                     /inference="alignment:Splign:2.1.0"
     exon            2944..3096
                     /gene="Col4a1"
                     /gene_synonym="Bru; Col4a-1; Raw; Svc"
                     /inference="alignment:Splign:2.1.0"
     exon            3097..3195
                     /gene="Col4a1"
                     /gene_synonym="Bru; Col4a-1; Raw; Svc"
                     /inference="alignment:Splign:2.1.0"
     exon            3196..3285
                     /gene="Col4a1"
                     /gene_synonym="Bru; Col4a-1; Raw; Svc"
                     /inference="alignment:Splign:2.1.0"
     exon            3286..3425
                     /gene="Col4a1"
                     /gene_synonym="Bru; Col4a-1; Raw; Svc"
                     /inference="alignment:Splign:2.1.0"
     exon            3426..3552
                     /gene="Col4a1"
                     /gene_synonym="Bru; Col4a-1; Raw; Svc"
                     /inference="alignment:Splign:2.1.0"
     exon            3553..3633
                     /gene="Col4a1"
                     /gene_synonym="Bru; Col4a-1; Raw; Svc"
                     /inference="alignment:Splign:2.1.0"
     exon            3634..3732
                     /gene="Col4a1"
                     /gene_synonym="Bru; Col4a-1; Raw; Svc"
                     /inference="alignment:Splign:2.1.0"
     exon            3733..3783
                     /gene="Col4a1"
                     /gene_synonym="Bru; Col4a-1; Raw; Svc"
                     /inference="alignment:Splign:2.1.0"
     exon            3784..3969
                     /gene="Col4a1"
                     /gene_synonym="Bru; Col4a-1; Raw; Svc"
                     /inference="alignment:Splign:2.1.0"
     exon            3970..4103
                     /gene="Col4a1"
                     /gene_synonym="Bru; Col4a-1; Raw; Svc"
                     /inference="alignment:Splign:2.1.0"
     exon            4104..4176
                     /gene="Col4a1"
                     /gene_synonym="Bru; Col4a-1; Raw; Svc"
                     /inference="alignment:Splign:2.1.0"
     exon            4177..4248
                     /gene="Col4a1"
                     /gene_synonym="Bru; Col4a-1; Raw; Svc"
                     /inference="alignment:Splign:2.1.0"
     exon            4249..4377
                     /gene="Col4a1"
                     /gene_synonym="Bru; Col4a-1; Raw; Svc"
                     /inference="alignment:Splign:2.1.0"
     exon            4378..4476
                     /gene="Col4a1"
                     /gene_synonym="Bru; Col4a-1; Raw; Svc"
                     /inference="alignment:Splign:2.1.0"
     exon            4477..4689
                     /gene="Col4a1"
                     /gene_synonym="Bru; Col4a-1; Raw; Svc"
                     /inference="alignment:Splign:2.1.0"
     exon            4690..4867
                     /gene="Col4a1"
                     /gene_synonym="Bru; Col4a-1; Raw; Svc"
                     /inference="alignment:Splign:2.1.0"
     exon            4868..4982
                     /gene="Col4a1"
                     /gene_synonym="Bru; Col4a-1; Raw; Svc"
                     /inference="alignment:Splign:2.1.0"
     exon            4983..5155
                     /gene="Col4a1"
                     /gene_synonym="Bru; Col4a-1; Raw; Svc"
                     /inference="alignment:Splign:2.1.0"
     exon            5156..6615
                     /gene="Col4a1"
                     /gene_synonym="Bru; Col4a-1; Raw; Svc"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6498..6503
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Col4a1"
                     /gene_synonym="Bru; Col4a-1; Raw; Svc"
                     /note="hexamer: AATAAA"
     polyA_site      6519
                     /gene="Col4a1"
                     /gene_synonym="Bru; Col4a-1; Raw; Svc"
                     /note="major polyA site"
     regulatory      6585..6590
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Col4a1"
                     /gene_synonym="Bru; Col4a-1; Raw; Svc"
                     /note="hexamer: AATAAA"
     polyA_site      6608
                     /gene="Col4a1"
                     /gene_synonym="Bru; Col4a-1; Raw; Svc"
                     /note="major polyA site"
ORIGIN      
        1 ttggagacgc cgcccgcgca ctgccgaggg gagggggcag cgccaacaaa ttggggagct
       61 cctcgggcgg cgcgctcagg tctctgcgca gagtcaccgc acccgggacg gtgagcaggg
      121 ctcacgcccg ggccctccga gactgagcac ctcgccgtgg cccacgggaa ctgggctgca
      181 tttccccgcc gtctctgctg gtcccctgcc gccccgggcg cgccaccatg gggccccggc
      241 tcagcgtctg gcttctgctg ctcttcgccg cccttctgct ccacgaggag cgcagccgag
      301 cagctgcgaa gggcgattgt ggtggctctg gctgtggaaa atgtgactgt catggcgtga
      361 agggacaaaa gggagaaaga ggcttgccag ggttgcaagg tgtcattgga tttcccggga
      421 tgcaaggacc tgaggggcca catggaccac caggacaaaa gggtgatgct ggagaacctg
      481 gacttcctgg cacaaaaggg acgaggggac cccctggagc agctggctac cctgggaatc
      541 ccggacttcc tggtattcct ggccaagatg gccctccagg tcccccaggt atcccaggat
      601 gcaacggtac aaagggagag agagggccgc tcggtcctcc tggcttgcct ggattcagcg
      661 gcaatcctgg accaccaggg ttaccaggaa tgaagggaga tcctggtgaa attctcggcc
      721 acgttcccgg aacactgctg aaaggggaga gaggatttcc tggtatcccc gggatgccgg
      781 gctcaccagg gttgccagga ctccagggac ccgtgggtcc tccgggattt actggaccac
      841 cgggtcctcc aggccctcct ggacctcctg gagaaaaggg ccagatgggc tccagcttcc
      901 aaggaccaaa aggtgacaag ggagagcaag gggtcagcgg tcccccggga gttcctggac
      961 aggcacaagt taaggaaaaa ggagactttg ccccaacagg agaaaagggt cagaaaggtg
     1021 aacctggatt tccgggagta ccaggatatg gagagaaagg tgaacctggc aagcaagggc
     1081 ctcggggaaa acctgggaaa gacggtgaaa aaggagaaag ggggagcccg ggcattcctg
     1141 gcgattccgg gtacccaggt ctcccaggcc ggcagggccc gcagggagag aagggtgaag
     1201 ctggacttcc aggcccccct ggaactgtga taggcaccat gcctttggga gagaaaggag
     1261 atcgaggcta cccaggagca ccagggttga gaggagagcc cggccctaaa ggtttccctg
     1321 gaacaccagg ccaaccaggc cctccaggtt tccctactcc aggccaggct ggtgctccag
     1381 gcttcccagg tgaaagggga gaaaaaggtg accagggatt tccaggcgtg tcattgccag
     1441 gaccaagtgg aagagatgga gccccggggc ctcccggccc tcccggcccc cctgggcagc
     1501 caggccacac aaatggcatt gtggagtgtc aacctggacc acccggggac cagggccctc
     1561 ctgggactcc aggacagcca ggtttgacag gtgaagttgg acagaaaggt cagaaaggag
     1621 agagctgcct tgcctgtgac acagaaggac ttcgtggccc tccagggcca caggggcctc
     1681 cgggagagat tggtttccct ggacaacctg gggctaaagg tgaccgaggc ctgcccggca
     1741 gagatggtct tgaaggattg ccgggtccac aaggttcacc agggcttata ggccagcctg
     1801 gagctaaggg agagcctgga gagatatttt ttgacatgcg actcaaaggt gacaaaggag
     1861 acccaggttt tccgggacag cctggcatgc caggaagagc aggaactccc ggaagagatg
     1921 gccacccagg actccctgga cccaaaggct ctccgggttc aataggattg aaaggagagc
     1981 gaggtcctcc cggaggagtt ggcttccctg gtagtcgtgg agacatcggc cctcctggac
     2041 ccccaggagt tggccccatt ggtcctgttg gtgaaaaagg acaagcaggc tttcctggag
     2101 gccctgggtc cccaggtctt ccaggcccaa agggtgaggc aggaaaggtt gtcccactcc
     2161 ctggcccccc aggagctgca ggacttccag gatcccctgg cttcccaggg ccacaaggtg
     2221 accgaggctt cccaggaacc ccaggacgtc caggcatccc gggagagaaa ggtgctgtgg
     2281 gccagccagg aattggattt cctgggcttc ctggccccaa aggtgttgat ggcttgcctg
     2341 gagagatagg acggcctggg agtccaggtc gccctggatt taacggctta cctggcaacc
     2401 caggacctca aggtcaaaag ggagaacctg gcattgggct tccaggactc aaagggcaac
     2461 caggccttcc aggcattccc ggtacacctg gagagaaggg tagcatcggg ggacccggcg
     2521 ttccaggaga acaggggttg acaggccccc caggactcca ggggatcaga ggtgacccag
     2581 ggcctcctgg agttcaaggc ccagcaggtc caccaggggt cccaggaata gggccacccg
     2641 gagctatggg ccctcccgga gggcaaggac caccagggtc atcaggtcca cctggaatta
     2701 aaggagagaa agggttccct ggattccccg gactggatat gcctggcccc aaaggcgata
     2761 aaggctctca aggacttcct ggcctcacag gacagtcagg cctccctggc cttcctggac
     2821 agcaggggac acctggagtt ccagggttcc caggttctaa aggtgaaatg ggtgtcatgg
     2881 gaaccccggg acaaccaggc tcgccaggac cagcaggcac cccagggtta cctggagaaa
     2941 aaggggacca tggccttccg ggctcctcag gacccagggg cgaccctggc ttcaaaggtg
     3001 ataaaggtga cgttgggctt cctggcatgc caggatccat ggagcatgtg gacatgggga
     3061 gcatgaaggg acagaaagga gaccagggag agaaaggaca aatcggaccc actggtgata
     3121 aaggttcccg aggagaccct ggaacaccag gagtacctgg gaaggatggg caggcagggc
     3181 atcccggaca gccagggcct aaaggtgacc caggccttag tgggacacca ggatcccctg
     3241 gactccctgg acccaaagga tcagttggag gaatgggctt gccaggttcg cctggagaaa
     3301 aaggtgtgcc tggcatccct ggctcacagg gtgtccctgg ctcacctgga gagaagggag
     3361 ccaaaggaga gaaagggcag tcaggtctac ctggcattgg gattccggga cggcctggtg
     3421 acaagggaga ccagggcctt gctggcttcc caggcagccc cggtgagaag ggagagaaag
     3481 gcagtgccgg aaccccaggg atgccagggt ccccaggccc gagaggttct ccggggaaca
     3541 tcggccatcc aggaagccca ggcctgcctg gagagaaagg ggataaaggc ctcccaggac
     3601 tggatggcgt tcctggtgtc aaaggagaag caggtctccc tgggactcct ggccccacag
     3661 gcccagctgg ccagaaggga gagccgggca gcgatggaat cccggggtcg gcaggagaga
     3721 agggtgaaca aggtgttcca ggaagaggct tccccggctt ccctggctcc aaaggagaca
     3781 aaggctccaa gggtgaagtg ggtttccctg gcctagctgg aagtcctgga attcctggag
     3841 tcaaaggcga gcaagggttc atgggtcctc ctggccctca aggacaacct ggcttacctg
     3901 gcactcctgg tcaccctgtg gaggggccca aaggagaccg aggacctcag ggtcaacctg
     3961 gcctgccagg gcatccggga cctatggggc cgccagggtt cccaggaatc aatgggccaa
     4021 aaggtgacaa gggaaatcaa ggttggccag gagctccggg ggttccaggc cctaagggag
     4081 acccaggatt ccaaggcatg ccgggcattg gcggctctcc agggatcaca ggttcaaagg
     4141 gagatatggg actgcccgga gttccaggat ttcaaggtca gaaagggctt cctggtctgc
     4201 agggagtgaa aggagaccag ggagaccaag gtgtacccgg ccctaaaggt ctccaaggtc
     4261 cccctgggcc tccaggtccc tacgatgtca tcaaaggaga accagggctc cctggtcctg
     4321 agggtccccc tggtcttaaa ggactccagg gaccaccagg tccaaaagga cagcaaggtg
     4381 tgacaggctc agtgggcttg cctggacctc caggtgtccc tgggttcgat ggtgctcctg
     4441 gccagaaagg agagacagga ccctttggac cacctggtcc aagagggttt cctggcccac
     4501 caggccccga tgggctgcca ggatccatgg gtcccccagg taccccatct gtggaccatg
     4561 gcttccttgt gaccaggcat agtcagacaa cagatgaccc actgtgtccc ccagggacca
     4621 aaattcttta ccatggatac tctctgctct atgtccaagg caacgagcgt gcccacgggc
     4681 aggacttggg tacggctggc agctgcctgc gtaagttcag caccatgccc tttctcttct
     4741 gcaacatcaa caacgtctgc aacttcgcct ccaggaacga ctactcttac tggctgtcca
     4801 cgccagagcc catgcccatg tccatggcac ccatctctgg ggacaacatc cggcccttca
     4861 ttagcaggtg tgcggtttgt gaagcaccgg ccatggtgat ggcggtacac agtcagacca
     4921 ttcagattcc gcagtgccct aacggttggt cctcactgtg gatcggctat tccttcgtga
     4981 tgcacaccag cgctggtgct gaaggttccg gccaagccct cgcatccccc gggtcctgtc
     5041 tggaagagtt tagaagcgcc ccattcatcg agtgccacgg cagaggaacg tgcaattact
     5101 acgcaaatgc ttacagcttt tggctcgcca ccatagagag aagcgagatg ttcaagaagc
     5161 ccacgccatc caccttgaag gcaggggagc tgcgaacaca cgtcagccgc tgccaagtgt
     5221 gcatgagaag aacataacta tgatgctcgc ctctgccacc gtcacaacat ggtgctactc
     5281 cccccccttg tcctcttctc cctcccattg tatttgtaac agcaatgaac cctagaatta
     5341 tatcctgtgt acctcactgt ccactatgaa aaccataaag tgccttatag ggattcgcgt
     5401 aactaacaca ccccgcttcg ttggcctctg tttgctgaag gagaaggaca ggcagcggag
     5461 agctttccat aatggcacat accaaatggc gctcctgatc caataaaaga tgaattgcat
     5521 tttgcaatcc agtgcactga tgtctggggc gagaactcca taagaaaccc aaaggtgcta
     5581 ggggcgtgag gcgctctcca cacagcccac agcccgcttc atcctgtcac cccagatgtg
     5641 agtgctgtcc aacccactgt ccagtgtttc cacatcaggt ccctcgaggg gctatgcaga
     5701 gaacagtcaa gggaactggt ttcttccact ttctacatgg aactccatcg tttcagcccc
     5761 aaacacaaac ttatgccctg tgtggtacta tgcagctgct tttgtggaag tcatggcttt
     5821 cctgtggaat aaagatggtc caaaggttag atggaaaata aataaataag taaataaata
     5881 tatatatata ttagtaattt atataggtgt cagcaattag gcaggtcaag ttctagcgta
     5941 gagcctgttg ttaaataaag cgcacttggt tttcgtctct ggagaaactg tactatcctt
     6001 tcacagggtt accaacatca ggccactgaa tgtgaaacac cagctttcct caaactcact
     6061 cctaaaccag aggaggtgta tagatagcca aagccaaacc cattcctaaa catggtgtct
     6121 gataatgagc cctgtgcctg gcgcttcttg cttctgctct gcgtggagta ttttcagggt
     6181 ttgcaacact aaccacagac tgaatgactg acttcccgta cgacagccaa ggcctttgtt
     6241 agcctccttc atcctgcatc ctggcaggca tttcacacct gagcacacac aggcaacttc
     6301 ccagctcctc tcgagctgtg acacagcccc tgcagggagt ggacaggtcg caggagggca
     6361 gactcggacc actatgcttg aagtgacatt tcactaaaag tacccaccct ccaaaaaaaa
     6421 aaaaaaaagt ttttaagact actaaggcct tctgtgtaat ttctttaaat gtgtatttct
     6481 taagaattca aatttgtaat aaaactattt gtataaaaat gaagctttta tggatttgtt
     6541 gctagaaatg aaacgttgaa agagaagtta ccacacgtac cactaataaa cctgtaagct
     6601 ttgctgcata ccttaaaaaa aaaaaaaaaa aaaaaa
//
