LOCUS       NM_031163               5053 bp    mRNA    linear   ROD 11-MAR-2025
DEFINITION  Mus musculus collagen, type II, alpha 1 (Col2a1), transcript
            variant 1, mRNA.
ACCESSION   NM_031163
VERSION     NM_031163.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 5053)
  AUTHORS   Song,C., Sawall,J.K., Ji,X., Song,F., Liao,X., Peng,R., Ren,H.,
            Koyama,E., Pacifici,M. and Long,F.
  TITLE     Tgfbeta signaling stimulates glycolysis to promote the genesis of
            synovial joint interzone in developing mouse embryonic limbs
  JOURNAL   Sci Adv 11 (2), eadq4991 (2025)
   PUBMED   39772668
REFERENCE   2  (bases 1 to 5053)
  AUTHORS   Hata,K., Wakamori,K., Hirakawa-Yamamura,A., Ichiyama-Kobayashi,S.,
            Yamaguchi,M., Okuzaki,D., Takahata,Y., Murakami,T., Uzawa,N.,
            Yamashiro,T. and Nishimura,R.
  TITLE     Serinc5 Regulates Sequential Chondrocyte Differentiation by
            Inhibiting Sox9 Function in Pre-Hypertrophic Chondrocytes
  JOURNAL   J Cell Physiol 240 (1), e31490 (2025)
   PUBMED   39568258
REFERENCE   3  (bases 1 to 5053)
  AUTHORS   Fritzsch,B., Weng,X., Yamoah,E.N., Qin,T., Hui,C.C.,
            Lebron-Mora,L., Pavlinkova,G. and Sham,M.H.
  TITLE     Irx3/5 Null Deletion in Mice Blocks Cochlea-Saccule Segregation and
            Disrupts the Auditory Tonotopic Map
  JOURNAL   J Comp Neurol 532 (12), e70008 (2024)
   PUBMED   39655644
REFERENCE   4  (bases 1 to 5053)
  AUTHORS   Dinesh,N.E.H., Baratang,N., Rosseau,J., Mohapatra,R., Li,L.,
            Mahalingam,R., Tiedemann,K., Campeau,P.M. and Reinhardt,D.P.
  TITLE     Fibronectin isoforms promote postnatal skeletal development
  JOURNAL   Matrix Biol 133, 86-102 (2024)
   PUBMED   39159790
REFERENCE   5  (bases 1 to 5053)
  AUTHORS   Cai,S. and Yin,N.
  TITLE     Single-cell transcriptome and chromatin accessibility mapping of
            upper lip and primary palate fusion
  JOURNAL   J Cell Mol Med 28 (19), e70128 (2024)
   PUBMED   39392189
REFERENCE   6  (bases 1 to 5053)
  AUTHORS   Andrikopoulos,K., Suzuki,H.R., Solursh,M. and Ramirez,F.
  TITLE     Localization of pro-alpha 2(V) collagen transcripts in the tissues
            of the developing mouse embryo
  JOURNAL   Dev Dyn 195 (2), 113-120 (1992)
   PUBMED   1297453
REFERENCE   7  (bases 1 to 5053)
  AUTHORS   Vikkula,M., Metsaranta,M., Syvanen,A.C., Ala-Kokko,L., Vuorio,E.
            and Peltonen,L.
  TITLE     Structural analysis of the regulatory elements of the type-II
            procollagen gene. Conservation of promoter and first intron
            sequences between human and mouse
  JOURNAL   Biochem J 285 (Pt 1) (Pt 1), 287-294 (1992)
   PUBMED   1637314
REFERENCE   8  (bases 1 to 5053)
  AUTHORS   Metsaranta,M., Garofalo,S., Decker,G., Rintala,M., de
            Crombrugghe,B. and Vuorio,E.
  TITLE     Chondrodysplasia in transgenic mice harboring a 15-amino acid
            deletion in the triple helical domain of pro alpha 1(II) collagen
            chain
  JOURNAL   J Cell Biol 118 (1), 203-212 (1992)
   PUBMED   1618904
REFERENCE   9  (bases 1 to 5053)
  AUTHORS   Cheah,K.S., Lau,E.T., Au,P.K. and Tam,P.P.
  TITLE     Expression of the mouse alpha 1(II) collagen gene is not restricted
            to cartilage during development
  JOURNAL   Development 111 (4), 945-953 (1991)
   PUBMED   1879363
REFERENCE   10 (bases 1 to 5053)
  AUTHORS   Cheah,K.S., Au,P.K., Lau,E.T., Little,P.F. and Stubbs,L.
  TITLE     The mouse Col2a-1 gene is highly conserved and is linked to Int-1
            on chromosome 15
  JOURNAL   Mamm Genome 1 (3), 171-183 (1991)
   PUBMED   1797232
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC113444.9 and AC134554.6.
            
            On Aug 6, 2022 this sequence version replaced NM_031163.3.
            
            Summary: This gene encodes the alpha-1 subunit of the
            fibril-forming type II collagen, the major component of cartilage
            and the vitreous humor of the eye. The encoded preproprotein forms
            homotrimeric, triple helical procollagen that undergoes proteolytic
            processing during fibirl formation. Mice harboring certain
            mutations in this gene exhibit severe chondrodysplasia
            characterized by short limbs and trunch, craniofacial deformities
            and cleft palate. A complete lack of the encoded protein in mice
            results in postnatal lethality. Alternative splicing results in
            multiple transcript variants encoding different isoforms that may
            undergo similar proteolytic processing. [provided by RefSeq, Dec
            2015].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC082331.1, SRR9219382.3452.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164137 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-153               AC113444.9         155863-156015
            154-236             AC134554.6         147947-148029       c
            237-440             AC134554.6         143941-144144       c
            441-457             AC134554.6         142813-142829       c
            458-490             AC134554.6         142392-142424       c
            491-523             AC134554.6         142237-142269       c
            524-577             AC134554.6         142083-142136       c
            578-682             AC134554.6         141837-141941       c
            683-760             AC134554.6         140850-140927       c
            761-805             AC134554.6         140186-140230       c
            806-859             AC134554.6         140027-140080       c
            860-913             AC134554.6         139641-139694       c
            914-967             AC134554.6         138898-138951       c
            968-1021            AC134554.6         138554-138607       c
            1022-1075           AC134554.6         138406-138459       c
            1076-1120           AC134554.6         138082-138126       c
            1121-1174           AC134554.6         137555-137608       c
            1175-1219           AC134554.6         134882-134926       c
            1220-1273           AC134554.6         134451-134504       c
            1274-1372           AC134554.6         132824-132922       c
            1373-1417           AC134554.6         132486-132530       c
            1418-1516           AC134554.6         132300-132398       c
            1517-1570           AC134554.6         132101-132154       c
            1571-1678           AC134554.6         131622-131729       c
            1679-1732           AC134554.6         131206-131259       c
            1733-1831           AC134554.6         131023-131121       c
            1832-1885           AC134554.6         130831-130884       c
            1886-1984           AC134554.6         130280-130378       c
            1985-2038           AC134554.6         129846-129899       c
            2039-2092           AC134554.6         129401-129454       c
            2093-2146           AC134554.6         129101-129154       c
            2147-2200           AC134554.6         128820-128873       c
            2201-2245           AC134554.6         128542-128586       c
            2246-2344           AC134554.6         128258-128356       c
            2345-2452           AC134554.6         127900-128007       c
            2453-2506           AC134554.6         127499-127552       c
            2507-2560           AC134554.6         127190-127243       c
            2561-2614           AC134554.6         126776-126829       c
            2615-2668           AC134554.6         126454-126507       c
            2669-2776           AC134554.6         126067-126174       c
            2777-2830           AC134554.6         125542-125595       c
            2831-2884           AC134554.6         125053-125106       c
            2885-3046           AC134554.6         124263-124424       c
            3047-3154           AC134554.6         123986-124093       c
            3155-3262           AC134554.6         123684-123791       c
            3263-3316           AC134554.6         123413-123466       c
            3317-3424           AC134554.6         123170-123277       c
            3425-3478           AC134554.6         122923-122976       c
            3479-3586           AC134554.6         122703-122810       c
            3587-3640           AC134554.6         122416-122469       c
            3641-3748           AC134554.6         121876-121983       c
            3749-4037           AC134554.6         121259-121547       c
            4038-4225           AC134554.6         120720-120907       c
            4226-4468           AC134554.6         120180-120422       c
            4469-5053           AC134554.6         119140-119724       c
FEATURES             Location/Qualifiers
     source          1..5053
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="15"
                     /map="15 53.97 cM"
     gene            1..5053
                     /gene="Col2a1"
                     /gene_synonym="Col2; Col2a; Col2a-1; Del1; Dmm; Lpk;
                     M100413; Rgsc413; Rgsc856"
                     /note="collagen, type II, alpha 1"
                     /db_xref="GeneID:12824"
                     /db_xref="MGI:MGI:88452"
     exon            1..236
                     /gene="Col2a1"
                     /gene_synonym="Col2; Col2a; Col2a-1; Del1; Dmm; Lpk;
                     M100413; Rgsc413; Rgsc856"
                     /inference="alignment:Splign:2.1.0"
     CDS             152..4615
                     /gene="Col2a1"
                     /gene_synonym="Col2; Col2a; Col2a-1; Del1; Dmm; Lpk;
                     M100413; Rgsc413; Rgsc856"
                     /note="isoform 1 preproprotein is encoded by transcript
                     variant 1; chondrocalcin; alpha-1 type II collagen;
                     procollagen, type II, alpha 1"
                     /codon_start=1
                     /product="collagen alpha-1(II) chain isoform 1
                     preproprotein"
                     /protein_id="NP_112440.2"
                     /db_xref="CCDS:CCDS37189.2"
                     /db_xref="GeneID:12824"
                     /db_xref="MGI:MGI:88452"
                     /translation="MIRLGAPQSLVLLTLLIAAVLRCQGQDAQEAGSCLQNGQRYKDK
                     DVWKPSSCRICVCDTGNVLCDDIICEDPDCLNPEIPFGECCPICPADLATASGKLGPK
                     GQKGEPGDIRDIIGPRGPPGPQGPAGEQGPRGDRGDKGEKGAPGPRGRDGEPGTPGNP
                     GPAGPPGPPGPPGLSAGNFAAQMAGGYDEKAGGAQMGVMQGPMGPMGPRGPPGPAGAP
                     GPQGFQGNPGEPGEPGVSGPMGPRGPPGPAGKPGDDGEAGKPGKSGERGLPGPQGARG
                     FPGTPGLPGVKGHRGYPGLDGAKGEAGAPGVKGESGSPGENGSPGPMGPRGLPGERGR
                     TGPAGAAGARGNDGQPGPAGPPGPVGPAGGPGFPGAPGAKGEAGPTGARGPEGAQGSR
                     GEPGNPGSPGPAGASGNPGTDGIPGAKGSAGAPGIAGAPGFPGPRGPPGPQGATGPLG
                     PKGQAGEPGIAGFKGDQGPKGETGPAGPQGAPGPAGEEGKRGARGEPGGAGPIGPPGE
                     RGAPGNRGFPGQDGLAGPKGAPGERGPSGLTGPKGANGDPGRPGEPGLPGARGLTGRP
                     GDAGPQGKVGPSGAPGEDGRPGPPGPQGARGQPGVMGFPGPKGANGEPGKAGEKGLAG
                     APGLRGLPGKDGETGAAGPPGPSGPAGERGEQGAPGPSGFQGLPGPPGPPGEGGKQGD
                     QGIPGEAGAPGLVGPRGERGFPGERGSPGAQGLQGPRGLPGTPGTDGPKGAAGPDGPP
                     GAQGPPGLQGMPGERGAAGIAGPKGDRGDVGEKGPEGAPGKDGGRGLTGPIGPPGPAG
                     ANGEKGEVGPPGPSGSTGARGAPGERGETGPPGPAGFAGPPGADGQPGAKGDQGEAGQ
                     KGDAGAPGPQGPSGAPGPQGPTGVTGPKGARGAQGPPGATGFPGAAGRVGPPGANGNP
                     GPAGPPGPAGKDGPKGVRGDSGPPGRAGDPGLQGPAGAPGEKGEPGDDGPSGLDGPPG
                     PQGLAGQRGIVGLPGQRGERGFPGLPGPSGEPGKQGAPGASGDRGPPGPVGPPGLTGP
                     AGEPGREGSPGADGPPGRDGAAGVKGDRGETGALGAPGAPGPPGSPGPAGPTGKQGDR
                     GEAGAQGPMGPSGPAGARGIAGPQGPRGDKGESGEQGERGLKGHRGFTGLQGLPGPPG
                     PSGDQGASGPAGPSGPRGPPGPVGPSGKDGSNGIPGPIGPPGPRGRSGETGPVGPPGS
                     PGPPGPPGPPGPGIDMSAFAGLGQREKGPDPMQYMRADEADSTLRQHDVEVDATLKSL
                     NNQIESIRSPDGSRKNPARTCQDLKLCHPEWKSGDYWIDPNQGCTLDAMKVFCNMETG
                     ETCVYPNPATVPRKNWWSSKSKEKKHIWFGETMNGGFHFSYGDGNLAPNTANVQMTFL
                     RLLSTEGSQNITYHCKNSIAYLDEAAGNLKKALLIQGSNDVEMRAEGNSRFTYTALKD
                     GCTKHTGKWGKTVIEYRSQKTSRLPIIDIAPMDIGGAEQEFGVDIGPVCFL"
     exon            237..440
                     /gene="Col2a1"
                     /gene_synonym="Col2; Col2a; Col2a-1; Del1; Dmm; Lpk;
                     M100413; Rgsc413; Rgsc856"
                     /inference="alignment:Splign:2.1.0"
     exon            441..457
                     /gene="Col2a1"
                     /gene_synonym="Col2; Col2a; Col2a-1; Del1; Dmm; Lpk;
                     M100413; Rgsc413; Rgsc856"
                     /inference="alignment:Splign:2.1.0"
     exon            458..490
                     /gene="Col2a1"
                     /gene_synonym="Col2; Col2a; Col2a-1; Del1; Dmm; Lpk;
                     M100413; Rgsc413; Rgsc856"
                     /inference="alignment:Splign:2.1.0"
     exon            491..523
                     /gene="Col2a1"
                     /gene_synonym="Col2; Col2a; Col2a-1; Del1; Dmm; Lpk;
                     M100413; Rgsc413; Rgsc856"
                     /inference="alignment:Splign:2.1.0"
     exon            524..577
                     /gene="Col2a1"
                     /gene_synonym="Col2; Col2a; Col2a-1; Del1; Dmm; Lpk;
                     M100413; Rgsc413; Rgsc856"
                     /inference="alignment:Splign:2.1.0"
     exon            578..682
                     /gene="Col2a1"
                     /gene_synonym="Col2; Col2a; Col2a-1; Del1; Dmm; Lpk;
                     M100413; Rgsc413; Rgsc856"
                     /inference="alignment:Splign:2.1.0"
     exon            683..760
                     /gene="Col2a1"
                     /gene_synonym="Col2; Col2a; Col2a-1; Del1; Dmm; Lpk;
                     M100413; Rgsc413; Rgsc856"
                     /inference="alignment:Splign:2.1.0"
     exon            761..805
                     /gene="Col2a1"
                     /gene_synonym="Col2; Col2a; Col2a-1; Del1; Dmm; Lpk;
                     M100413; Rgsc413; Rgsc856"
                     /inference="alignment:Splign:2.1.0"
     exon            806..859
                     /gene="Col2a1"
                     /gene_synonym="Col2; Col2a; Col2a-1; Del1; Dmm; Lpk;
                     M100413; Rgsc413; Rgsc856"
                     /inference="alignment:Splign:2.1.0"
     exon            860..913
                     /gene="Col2a1"
                     /gene_synonym="Col2; Col2a; Col2a-1; Del1; Dmm; Lpk;
                     M100413; Rgsc413; Rgsc856"
                     /inference="alignment:Splign:2.1.0"
     exon            914..967
                     /gene="Col2a1"
                     /gene_synonym="Col2; Col2a; Col2a-1; Del1; Dmm; Lpk;
                     M100413; Rgsc413; Rgsc856"
                     /inference="alignment:Splign:2.1.0"
     exon            968..1021
                     /gene="Col2a1"
                     /gene_synonym="Col2; Col2a; Col2a-1; Del1; Dmm; Lpk;
                     M100413; Rgsc413; Rgsc856"
                     /inference="alignment:Splign:2.1.0"
     exon            1022..1075
                     /gene="Col2a1"
                     /gene_synonym="Col2; Col2a; Col2a-1; Del1; Dmm; Lpk;
                     M100413; Rgsc413; Rgsc856"
                     /inference="alignment:Splign:2.1.0"
     exon            1076..1120
                     /gene="Col2a1"
                     /gene_synonym="Col2; Col2a; Col2a-1; Del1; Dmm; Lpk;
                     M100413; Rgsc413; Rgsc856"
                     /inference="alignment:Splign:2.1.0"
     exon            1121..1174
                     /gene="Col2a1"
                     /gene_synonym="Col2; Col2a; Col2a-1; Del1; Dmm; Lpk;
                     M100413; Rgsc413; Rgsc856"
                     /inference="alignment:Splign:2.1.0"
     exon            1175..1219
                     /gene="Col2a1"
                     /gene_synonym="Col2; Col2a; Col2a-1; Del1; Dmm; Lpk;
                     M100413; Rgsc413; Rgsc856"
                     /inference="alignment:Splign:2.1.0"
     exon            1220..1273
                     /gene="Col2a1"
                     /gene_synonym="Col2; Col2a; Col2a-1; Del1; Dmm; Lpk;
                     M100413; Rgsc413; Rgsc856"
                     /inference="alignment:Splign:2.1.0"
     exon            1274..1372
                     /gene="Col2a1"
                     /gene_synonym="Col2; Col2a; Col2a-1; Del1; Dmm; Lpk;
                     M100413; Rgsc413; Rgsc856"
                     /inference="alignment:Splign:2.1.0"
     exon            1373..1417
                     /gene="Col2a1"
                     /gene_synonym="Col2; Col2a; Col2a-1; Del1; Dmm; Lpk;
                     M100413; Rgsc413; Rgsc856"
                     /inference="alignment:Splign:2.1.0"
     exon            1418..1516
                     /gene="Col2a1"
                     /gene_synonym="Col2; Col2a; Col2a-1; Del1; Dmm; Lpk;
                     M100413; Rgsc413; Rgsc856"
                     /inference="alignment:Splign:2.1.0"
     exon            1517..1570
                     /gene="Col2a1"
                     /gene_synonym="Col2; Col2a; Col2a-1; Del1; Dmm; Lpk;
                     M100413; Rgsc413; Rgsc856"
                     /inference="alignment:Splign:2.1.0"
     exon            1571..1678
                     /gene="Col2a1"
                     /gene_synonym="Col2; Col2a; Col2a-1; Del1; Dmm; Lpk;
                     M100413; Rgsc413; Rgsc856"
                     /inference="alignment:Splign:2.1.0"
     exon            1679..1732
                     /gene="Col2a1"
                     /gene_synonym="Col2; Col2a; Col2a-1; Del1; Dmm; Lpk;
                     M100413; Rgsc413; Rgsc856"
                     /inference="alignment:Splign:2.1.0"
     exon            1733..1831
                     /gene="Col2a1"
                     /gene_synonym="Col2; Col2a; Col2a-1; Del1; Dmm; Lpk;
                     M100413; Rgsc413; Rgsc856"
                     /inference="alignment:Splign:2.1.0"
     exon            1832..1885
                     /gene="Col2a1"
                     /gene_synonym="Col2; Col2a; Col2a-1; Del1; Dmm; Lpk;
                     M100413; Rgsc413; Rgsc856"
                     /inference="alignment:Splign:2.1.0"
     exon            1886..1984
                     /gene="Col2a1"
                     /gene_synonym="Col2; Col2a; Col2a-1; Del1; Dmm; Lpk;
                     M100413; Rgsc413; Rgsc856"
                     /inference="alignment:Splign:2.1.0"
     exon            1985..2038
                     /gene="Col2a1"
                     /gene_synonym="Col2; Col2a; Col2a-1; Del1; Dmm; Lpk;
                     M100413; Rgsc413; Rgsc856"
                     /inference="alignment:Splign:2.1.0"
     exon            2039..2092
                     /gene="Col2a1"
                     /gene_synonym="Col2; Col2a; Col2a-1; Del1; Dmm; Lpk;
                     M100413; Rgsc413; Rgsc856"
                     /inference="alignment:Splign:2.1.0"
     exon            2093..2146
                     /gene="Col2a1"
                     /gene_synonym="Col2; Col2a; Col2a-1; Del1; Dmm; Lpk;
                     M100413; Rgsc413; Rgsc856"
                     /inference="alignment:Splign:2.1.0"
     exon            2147..2200
                     /gene="Col2a1"
                     /gene_synonym="Col2; Col2a; Col2a-1; Del1; Dmm; Lpk;
                     M100413; Rgsc413; Rgsc856"
                     /inference="alignment:Splign:2.1.0"
     exon            2201..2245
                     /gene="Col2a1"
                     /gene_synonym="Col2; Col2a; Col2a-1; Del1; Dmm; Lpk;
                     M100413; Rgsc413; Rgsc856"
                     /inference="alignment:Splign:2.1.0"
     exon            2246..2344
                     /gene="Col2a1"
                     /gene_synonym="Col2; Col2a; Col2a-1; Del1; Dmm; Lpk;
                     M100413; Rgsc413; Rgsc856"
                     /inference="alignment:Splign:2.1.0"
     exon            2345..2452
                     /gene="Col2a1"
                     /gene_synonym="Col2; Col2a; Col2a-1; Del1; Dmm; Lpk;
                     M100413; Rgsc413; Rgsc856"
                     /inference="alignment:Splign:2.1.0"
     exon            2453..2506
                     /gene="Col2a1"
                     /gene_synonym="Col2; Col2a; Col2a-1; Del1; Dmm; Lpk;
                     M100413; Rgsc413; Rgsc856"
                     /inference="alignment:Splign:2.1.0"
     exon            2507..2560
                     /gene="Col2a1"
                     /gene_synonym="Col2; Col2a; Col2a-1; Del1; Dmm; Lpk;
                     M100413; Rgsc413; Rgsc856"
                     /inference="alignment:Splign:2.1.0"
     exon            2561..2614
                     /gene="Col2a1"
                     /gene_synonym="Col2; Col2a; Col2a-1; Del1; Dmm; Lpk;
                     M100413; Rgsc413; Rgsc856"
                     /inference="alignment:Splign:2.1.0"
     exon            2615..2668
                     /gene="Col2a1"
                     /gene_synonym="Col2; Col2a; Col2a-1; Del1; Dmm; Lpk;
                     M100413; Rgsc413; Rgsc856"
                     /inference="alignment:Splign:2.1.0"
     exon            2669..2776
                     /gene="Col2a1"
                     /gene_synonym="Col2; Col2a; Col2a-1; Del1; Dmm; Lpk;
                     M100413; Rgsc413; Rgsc856"
                     /inference="alignment:Splign:2.1.0"
     exon            2777..2830
                     /gene="Col2a1"
                     /gene_synonym="Col2; Col2a; Col2a-1; Del1; Dmm; Lpk;
                     M100413; Rgsc413; Rgsc856"
                     /inference="alignment:Splign:2.1.0"
     exon            2831..2884
                     /gene="Col2a1"
                     /gene_synonym="Col2; Col2a; Col2a-1; Del1; Dmm; Lpk;
                     M100413; Rgsc413; Rgsc856"
                     /inference="alignment:Splign:2.1.0"
     exon            2885..3046
                     /gene="Col2a1"
                     /gene_synonym="Col2; Col2a; Col2a-1; Del1; Dmm; Lpk;
                     M100413; Rgsc413; Rgsc856"
                     /inference="alignment:Splign:2.1.0"
     exon            3047..3154
                     /gene="Col2a1"
                     /gene_synonym="Col2; Col2a; Col2a-1; Del1; Dmm; Lpk;
                     M100413; Rgsc413; Rgsc856"
                     /inference="alignment:Splign:2.1.0"
     exon            3155..3262
                     /gene="Col2a1"
                     /gene_synonym="Col2; Col2a; Col2a-1; Del1; Dmm; Lpk;
                     M100413; Rgsc413; Rgsc856"
                     /inference="alignment:Splign:2.1.0"
     exon            3263..3316
                     /gene="Col2a1"
                     /gene_synonym="Col2; Col2a; Col2a-1; Del1; Dmm; Lpk;
                     M100413; Rgsc413; Rgsc856"
                     /inference="alignment:Splign:2.1.0"
     exon            3317..3424
                     /gene="Col2a1"
                     /gene_synonym="Col2; Col2a; Col2a-1; Del1; Dmm; Lpk;
                     M100413; Rgsc413; Rgsc856"
                     /inference="alignment:Splign:2.1.0"
     exon            3425..3478
                     /gene="Col2a1"
                     /gene_synonym="Col2; Col2a; Col2a-1; Del1; Dmm; Lpk;
                     M100413; Rgsc413; Rgsc856"
                     /inference="alignment:Splign:2.1.0"
     exon            3479..3586
                     /gene="Col2a1"
                     /gene_synonym="Col2; Col2a; Col2a-1; Del1; Dmm; Lpk;
                     M100413; Rgsc413; Rgsc856"
                     /inference="alignment:Splign:2.1.0"
     exon            3587..3640
                     /gene="Col2a1"
                     /gene_synonym="Col2; Col2a; Col2a-1; Del1; Dmm; Lpk;
                     M100413; Rgsc413; Rgsc856"
                     /inference="alignment:Splign:2.1.0"
     exon            3641..3748
                     /gene="Col2a1"
                     /gene_synonym="Col2; Col2a; Col2a-1; Del1; Dmm; Lpk;
                     M100413; Rgsc413; Rgsc856"
                     /inference="alignment:Splign:2.1.0"
     exon            3749..4037
                     /gene="Col2a1"
                     /gene_synonym="Col2; Col2a; Col2a-1; Del1; Dmm; Lpk;
                     M100413; Rgsc413; Rgsc856"
                     /inference="alignment:Splign:2.1.0"
     exon            4038..4225
                     /gene="Col2a1"
                     /gene_synonym="Col2; Col2a; Col2a-1; Del1; Dmm; Lpk;
                     M100413; Rgsc413; Rgsc856"
                     /inference="alignment:Splign:2.1.0"
     exon            4226..4468
                     /gene="Col2a1"
                     /gene_synonym="Col2; Col2a; Col2a-1; Del1; Dmm; Lpk;
                     M100413; Rgsc413; Rgsc856"
                     /inference="alignment:Splign:2.1.0"
     exon            4469..5053
                     /gene="Col2a1"
                     /gene_synonym="Col2; Col2a; Col2a-1; Del1; Dmm; Lpk;
                     M100413; Rgsc413; Rgsc856"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4799..4804
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Col2a1"
                     /gene_synonym="Col2; Col2a; Col2a-1; Del1; Dmm; Lpk;
                     M100413; Rgsc413; Rgsc856"
                     /note="hexamer: AATAAA"
     polyA_site      4822
                     /gene="Col2a1"
                     /gene_synonym="Col2; Col2a; Col2a-1; Del1; Dmm; Lpk;
                     M100413; Rgsc413; Rgsc856"
                     /note="major polyA site"
ORIGIN      
        1 gcagagcgcc gctgggctgc cgggtctcct gcctcctcct gctcctaggg cctcctgcat
       61 gagggagcgg tagagacccg gacccgctcc gtgctctgcc gcctcgctgc gcttcgcccg
      121 ggccaggctc tgccaggcct cgcggtgagc catgatccgc ctcggggctc cccagtcgct
      181 ggtgctgctg acgctgctca tcgccgcggt cctacggtgt cagggccagg atgcccagga
      241 ggctggcagc tgtctgcaga atgggcagag gtataaagat aaggatgtat ggaagccctc
      301 atcttgccgc atctgtgtgt gtgacactgg gaatgtcctc tgcgatgaca ttatctgtga
      361 agacccagac tgcctcaacc ccgagatccc cttcggagag tgctgtccca tctgcccagc
      421 tgacctcgcc actgccagtg gaaaattagg gccaaagggg cagaaaggag aacctggaga
      481 tatcagagat atcataggac ccagaggacc tcctggccct cagggacctg caggtgaaca
      541 aggacccaga ggtgatcgtg gtgacaaggg agaaaagggt gcgcctggac cccgtggcag
      601 agatggagaa cctggtaccc ctggaaatcc tggccccgct ggccctccag gtccccctgg
      661 tccccctggc cttagtgcag gaaacttcgc ggctcagatg gctggagggt atgacgagaa
      721 ggctggtggt gcccagatgg gagtcatgca agggcccatg ggccccatgg gaccccgtgg
      781 acccccaggc cctgccggtg cccccggccc tcaaggattt caaggcaatc ctggtgaacc
      841 tggcgagcct ggtgtctctg gtcccatggg tccccgaggt cctcctggcc ctgctggaaa
      901 acctggtgac gacggtgaag ctgggaagcc cggaaagtct ggggaaagag gcctccctgg
      961 ccctcagggt gctcgtggat tcccaggaac cccgggtctc cccggtgtca agggtcacag
     1021 aggttaccca ggcctcgacg gtgctaaggg ggaagctggt gctccgggtg tgaagggtga
     1081 gagtggttcc cctggtgaga acggatcccc gggcccaatg ggtccccgtg gcctgcctgg
     1141 tgagagagga cggactggcc ctgctggtgc tgctggtgct cggggtaacg atggccagcc
     1201 aggccccgct ggacctccgg gtcctgtggg tcccgcaggt ggtcctggct tccctggtgc
     1261 tcctggtgcc aagggcgaag ctggtcccac tggtgctcgc ggtcctgaag gtgctcaagg
     1321 ttctcgtggc gagcctggca atcctgggtc ccctgggcct gcaggtgctt ctggtaaccc
     1381 agggactgat ggtattcctg gagccaaagg atccgctggt gctcctggaa ttgctggtgc
     1441 ccctggcttc cctgggcccc gtggccctcc cggtcctcaa ggtgcaactg gtccccttgg
     1501 ccccaaaggt caggcgggtg aacctggcat tgctggcttt aaaggtgatc aaggccccaa
     1561 gggagagact ggacctgctg ggccccaagg agcccctggc cccgctggtg aagaaggcaa
     1621 acgaggtgct cgaggagagc cgggtggtgc tggaccaatc ggaccccctg gagagagagg
     1681 tgctcctggc aaccgtggat tcccaggtca agatggtctg gcaggtccca agggtgcccc
     1741 tggagagcga gggcccagtg gcttgactgg tcccaaggga gccaacggtg acccgggtcg
     1801 tcctggagaa cctggtcttc ctggagccag gggtcttacc ggtcgccctg gtgacgctgg
     1861 tcctcaaggc aaagttggtc cttctggagc ccctggtgaa gacggtcgcc ctggacctcc
     1921 tggtcctcag ggagctcgtg ggcagcctgg cgtcatgggt ttccctggcc ccaaaggtgc
     1981 caacggcgag cctggcaaag ctggtgagaa gggtctggct ggcgctcctg gtctgagagg
     2041 tcttcctgga aaagacggtg agacgggagc cgcaggaccc cccggcccca gtggacctgc
     2101 tggtgaacga ggcgagcagg gcgctcctgg accatcaggg ttccagggac ttcctggccc
     2161 tcccggtccc ccaggtgaag gtggaaagca aggtgaccag ggtattcctg gtgaagctgg
     2221 agctcctggc cttgtgggtc ctcggggcga gcgaggtttc ccaggtgaac gtggctctcc
     2281 tggtgctcag ggccttcagg gtccccgagg cctccctggc actcctggta ctgatggtcc
     2341 caaaggtgca gctggcccag atggcccccc tggggctcag gggcctccag gtctacaggg
     2401 aatgcctggt gagagaggag ccgctggcat tgctgggccc aagggagaca gaggcgatgt
     2461 tggcgagaaa ggcccagagg gagctcctgg gaaggatggc ggccgaggtc tgactgggcc
     2521 catcggaccc ccaggcccag caggggccaa cggcgagaag ggagaagtcg gacctcctgg
     2581 cccgtcagga agtaccggag ctcgaggtgc cccgggtgaa cgcggagaga ccgggccacc
     2641 tggacctgct ggattcgctg gccctcctgg tgctgatggc cagcctggtg ccaagggtga
     2701 tcaaggagaa gccggacaga aaggagatgc tggtgccccc ggcccacaag gcccctcggg
     2761 agcccctggg ccacagggtc ctactggagt gactggtcct aagggagccc gaggtgccca
     2821 aggtcccccg ggagccaccg gattccctgg agctgctggc cgagttggac ccccaggtgc
     2881 taatggcaat cctggacccg ccggtccccc tggtcctgct ggaaaagatg gtcccaaagg
     2941 tgttcgagga gacagtggcc cccctggcag agctggtgac cccggtcttc aaggtcctgc
     3001 aggagctcct ggcgagaaag gagaacctgg agatgatggt ccctctggtc ttgatggtcc
     3061 tccaggtccc caggggctgg ctggtcaaag gggcattgtt ggtctgcctg gtcagcgtgg
     3121 tgagagagga ttccccggcc ttcccggccc atcgggtgag cccggcaagc agggtgcacc
     3181 tggcgcgtct ggagacagag gtcctcctgg tcctgtgggg cctcctggcc tgacagggcc
     3241 tgcaggtgaa cctggacgag agggcagccc tggtgctgat ggaccccctg gaagagatgg
     3301 tgcagctgga gtcaagggag atcgtggtga gactggagca ctgggtgccc ctggagctcc
     3361 tgggccccca ggctctcctg gtcctgctgg cccaactggc aaacaaggag acagaggaga
     3421 ggctggtgca caaggtccta tgggtccctc aggacctgct ggagcccgtg ggattgcagg
     3481 ccctcaaggc ccccgaggtg acaaaggaga atctggagag cagggcgaga ggggactgaa
     3541 gggacaccga ggtttcactg gactgcaggg tctgcctggc cctccgggtc cttctggaga
     3601 tcagggtgct tctggccctg ctggtccttc tggccctaga ggtccacctg gccctgttgg
     3661 tccctctggc aaagatggct ctaatggaat ccctggcccc atcgggcctc caggtccccg
     3721 tggacgctca ggagaaacag gccctgttgg tccccctgga agtcccggtc ctcctggccc
     3781 tccaggtcct cctggtcctg gcatcgacat gtcagccttt gctggcttag ggcagagaga
     3841 gaagggcccc gaccccatgc agtacatgcg ggccgacgag gcagacagta ccttgagaca
     3901 gcacgacgtg gaggtggacg ctacactcaa gtcactgaac aaccagattg agagcatccg
     3961 cagccccgac ggctcccgca agaaccctgc tcgcacttgc caagacctga aactctgcca
     4021 ccccgagtgg aagagcggag actactggat tgatcccaac cagggctgca ccttggacgc
     4081 catgaaagtt ttctgcaaca tggagaccgg cgagacttgc gtctacccca acccagcgac
     4141 tgtccctcgg aaaaactggt ggagcagcaa gagcaaggaa aagaaacaca tctggtttgg
     4201 agagaccatg aacggtggct tccacttcag ctatggcgat ggcaacctgg ctcccaacac
     4261 cgctaacgtc cagatgactt tcctccgtct actgtccact gagggctccc agaacatcac
     4321 ctaccactgt aagaacagca tcgcctacct ggacgaagcg gctggcaacc tcaagaaggc
     4381 cttgctcatc cagggctcca atgatgtaga gatgagggcc gagggcaaca gcaggttcac
     4441 atacactgcc ctgaaggatg gctgcacgaa acacactggt aagtggggca agaccgtcat
     4501 cgagtaccga tcacagaaga cctcccgcct tcccattatt gacatcgcac ccatggacat
     4561 tggaggggct gaacaggaat ttggtgtgga catagggcct gtctgcttct tgtaaaaccc
     4621 ccgaaccctg aaacaacaca atccattgcg aacccaaagg acccaaacac tttccaaccg
     4681 cagtcactcc aggatctgca ctgaatggct gacctgacct gatgataccc aaccgtcctc
     4741 ccctcacagc ccggactgtg ctcccctttc taagagacct gaactgggca gactgcaaaa
     4801 taaaatctcg gtgttctatt tatttattgt cttcctgtaa gacctctggg tccaggcgga
     4861 gacaggaact atctggtgtg agtcagacgc cccccgagtg actgttccca gcccagccag
     4921 aagaccccta cagatgctgg gcgcagggac tgcgtgtcct acacaatggt gctattctgt
     4981 gtcaaacacc tctgtatttt ttaaaacatc aattgatatt aaaaaccaaa aaaaaaaaaa
     5041 tcattggaaa gga
//
