LOCUS       NM_001109991            5608 bp    mRNA    linear   ROD 14-JAN-2025
DEFINITION  Mus musculus collagen, type XVIII, alpha 1 (Col18a1), transcript
            variant 1, mRNA.
ACCESSION   NM_001109991
VERSION     NM_001109991.2
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 5608)
  AUTHORS   Kay,R.G.G., Reeves,R., Siggers,P., Greenaway,S., Mallon,A.M.,
            Wells,S., Koo,B.K., Mayere,C., Nef,S., Greenfield,A. and Simon,M.M.
  TITLE     Gonadal sex reversal at single-cell resolution in Znrf3-deficient
            mice
  JOURNAL   Development 151 (23) (2024)
   PUBMED   39629665
REFERENCE   2  (bases 1 to 5608)
  AUTHORS   Patel,V.N., Aure,M.H., Choi,S.H., Ball,J.R., Lane,E.D., Wang,Z.,
            Xu,Y., Zheng,C., Liu,X., Martin,D., Pailin,J.Y., Prochazkova,M.,
            Kulkarni,A.B., van Kuppevelt,T.H., Ambudkar,I.S., Liu,J. and
            Hoffman,M.P.
  TITLE     Specific 3-O-sulfated heparan sulfate domains regulate salivary
            gland basement membrane metabolism and epithelial differentiation
  JOURNAL   Nat Commun 15 (1), 7584 (2024)
   PUBMED   39217171
  REMARK    Erratum:[Nat Commun. 2024 Oct 10;15(1):8784. doi:
            10.1038/s41467-024-53230-4. PMID: 39389981]
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 5608)
  AUTHORS   Rinta-Jaskari,M.M., Naillat,F., Ruotsalainen,H.J., Ronkainen,V.P.,
            Heljasvaara,R., Akram,S.U., Izzi,V., Miinalainen,I., Vainio,S.J.
            and Pihlajaniemi,T.A.
  TITLE     Collagen XVIII regulates extracellular matrix integrity in the
            developing nephrons and impacts nephron progenitor cell behavior
  JOURNAL   Matrix Biol 131, 30-45 (2024)
   PUBMED   38788809
REFERENCE   4  (bases 1 to 5608)
  AUTHORS   Khoshneviszadeh,M., Henneicke,S., Pirici,D., Senthilnathan,A.,
            Morton,L., Arndt,P., Kaushik,R., Norman,O., Jukkola,J., Dunay,I.R.,
            Seidenbecher,C., Heikkinen,A., Schreiber,S. and Dityatev,A.
  TITLE     Microvascular damage, neuroinflammation and extracellular matrix
            remodeling in Col18a1 knockout mice as a model for early cerebral
            small vessel disease
  JOURNAL   Matrix Biol 128, 39-64 (2024)
   PUBMED   38387749
  REMARK    GeneRIF: Microvascular damage, neuroinflammation and extracellular
            matrix remodeling in Col18a1 knockout mice as a model for early
            cerebral small vessel disease.
REFERENCE   5  (bases 1 to 5608)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   6  (bases 1 to 5608)
  AUTHORS   Muragaki,Y., Timmons,S., Griffith,C.M., Oh,S.P., Fadel,B.,
            Quertermous,T. and Olsen,B.R.
  TITLE     Mouse Col18a1 is expressed in a tissue-specific manner as three
            alternative variants and is localized in basement membrane zones
  JOURNAL   Proc Natl Acad Sci U S A 92 (19), 8763-8767 (1995)
   PUBMED   7568013
REFERENCE   7  (bases 1 to 5608)
  AUTHORS   Mitchell,R., Bacher,M., Bernhagen,J., Pushkarskaya,T., Seldin,M.F.
            and Bucala,R.
  TITLE     Cloning and characterization of the gene for mouse macrophage
            migration inhibitory factor (MIF)
  JOURNAL   J Immunol 154 (8), 3863-3870 (1995)
   PUBMED   7706726
REFERENCE   8  (bases 1 to 5608)
  AUTHORS   Rehn,M. and Pihlajaniemi,T.
  TITLE     Identification of three N-terminal ends of type XVIII collagen
            chains and tissue-specific differences in the expression of the
            corresponding transcripts. The longest form contains a novel motif
            homologous to rat and Drosophila frizzled proteins
  JOURNAL   J Biol Chem 270 (9), 4705-4711 (1995)
   PUBMED   7876242
REFERENCE   9  (bases 1 to 5608)
  AUTHORS   Rehn,M. and Pihlajaniemi,T.
  TITLE     Alpha 1(XVIII), a collagen chain with frequent interruptions in the
            collagenous sequence, a distinct tissue distribution, and homology
            with type XV collagen
  JOURNAL   Proc Natl Acad Sci U S A 91 (10), 4234-4238 (1994)
   PUBMED   8183894
REFERENCE   10 (bases 1 to 5608)
  AUTHORS   Oh,S.P., Kamagata,Y., Muragaki,Y., Timmons,S., Ooshima,A. and
            Olsen,B.R.
  TITLE     Isolation and sequencing of cDNAs for proteins with multiple
            domains of Gly-Xaa-Yaa repeats identify a distinct family of
            collagenous proteins
  JOURNAL   Proc Natl Acad Sci U S A 91 (10), 4229-4233 (1994)
   PUBMED   8183893
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC159334.9 and AC055777.12.
            
            On May 11, 2022 this sequence version replaced NM_001109991.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC043697.1, SRR10223970.12540.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849382, SAMN00849386
                                           [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-727               AC159334.9         31066-31792         c
            728-1272            AC159334.9         14098-14642         c
            1273-1359           AC055777.12        6249-6335
            1360-1413           AC055777.12        7776-7829
            1414-1543           AC055777.12        9809-9938
            1544-1620           AC055777.12        13980-14056
            1621-1806           AC055777.12        14341-14526
            1807-1833           AC055777.12        15918-15944
            1834-1896           AC055777.12        16686-16748
            1897-1974           AC055777.12        16852-16929
            1975-2028           AC055777.12        17153-17206
            2029-2187           AC055777.12        17363-17521
            2188-2250           AC055777.12        17984-18046
            2251-2277           AC055777.12        19031-19057
            2278-2409           AC055777.12        20724-20855
            2410-2472           AC055777.12        21403-21465
            2473-2499           AC055777.12        22237-22263
            2500-2535           AC055777.12        23143-23178
            2536-2607           AC055777.12        23530-23601
            2608-2643           AC055777.12        23859-23894
            2644-2733           AC055777.12        24155-24244
            2734-2763           AC055777.12        25226-25255
            2764-2790           AC055777.12        25359-25385
            2791-2853           AC055777.12        25707-25769
            2854-2928           AC055777.12        26741-26815
            2929-2955           AC055777.12        27547-27573
            2956-3009           AC055777.12        27773-27826
            3010-3084           AC055777.12        28181-28255
            3085-3153           AC055777.12        29902-29970
            3154-3196           AC055777.12        30626-30668
            3197-3259           AC055777.12        31164-31226
            3260-3303           AC055777.12        34793-34836
            3304-3432           AC055777.12        35182-35310
            3433-3577           AC055777.12        35803-35947
            3578-3651           AC055777.12        36027-36100
            3652-3783           AC055777.12        36375-36506
            3784-3816           AC055777.12        37804-37836
            3817-4065           AC055777.12        39378-39626
            4066-4263           AC055777.12        40224-40421
            4264-4379           AC055777.12        40935-41050
            4380-5608           AC055777.12        41798-43026
FEATURES             Location/Qualifiers
     source          1..5608
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="10"
                     /map="10 39.72 cM"
     gene            1..5608
                     /gene="Col18a1"
                     /note="collagen, type XVIII, alpha 1"
                     /db_xref="GeneID:12822"
                     /db_xref="MGI:MGI:88451"
     exon            1..727
                     /gene="Col18a1"
                     /inference="alignment:Splign:2.1.0"
     CDS             10..4593
                     /gene="Col18a1"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; collagen alpha-1(XVIII) chain; endostatin;
                     procollagen, type XVIII, alpha 1; alpha-1(XVIII) collagen"
                     /codon_start=1
                     /product="collagen alpha-1(XVIII) chain isoform 1
                     precursor"
                     /protein_id="NP_001103461.1"
                     /db_xref="CCDS:CCDS48604.1"
                     /db_xref="GeneID:12822"
                     /db_xref="MGI:MGI:88451"
                     /translation="MAPDPSRRLCLLLLLLLSCRLVPASADGNSLSPLNPLVWLWPPK
                     TSDSLEGPVSKPQNSSPVQSTENPTTHVVPQDGLTEQQTTPASSELPPEEEEEEDQKA
                     GQGGSPATPAVPIPLVAPAASPDMKEENVAGVGAKILNVAQGIRSFVQLWDEDSTIGH
                     SAGTEVPDSSIPTVLPSPAELSSAPQGSKTTLWLSSAIPSSPDAQTTEAGTLAVPTQL
                     PPFQSNLQAPLGRPSAPPDFPENVAEEVGLLQLLGDPLPEKISQIDDPHVGPAYIFGP
                     DSNSGQVAQYHFPKLFFRDFSLLFHVRPATEAAGVLFAITDAAQVVVSLGVKLSEVRD
                     GQQNISLLYTEPGASQTQTGASFRLPAFVGQWTHFALSVDGGSVALYVDCEEFQRVPF
                     ARASQGLELERGAGLFVGQAGTADPDKFQGMISELKVRKTPRVSPVHCLDEEDDDEDR
                     ASGDFGSGFEESSKSHKEDTSLLPGLPQPPPVTSPPLAGGSTTEDPRTEETEEDAAVD
                     SIGAETLPGTGSSGAWDEAIQNPGRGLIKGGMKGQKGEPGAQGPPGPAGPQGPAGPVV
                     QSPNSQPVPGAQGPPGPQGPPGKDGTPGRDGEPGDPGEDGRPGDTGPQGFPGTPGDVG
                     PKGEKGDPGIGPRGPPGPPGPPGPSFRQDKLTFIDMEGSGFSGDIESLRGPRGFPGPP
                     GPPGVPGLPGEPGRFGINGSYAPGPAGLPGVPGKEGPPGFPGPPGPPGPPGKEGPPGV
                     AGQKGSVGDVGIPGPKGSKGDLGPIGMPGKSGLAGSPGPVGPPGPPGPPGPPGPGFAA
                     GFDDMEGSGIPLWTTARSSDGLQGPPGSPGLKGDPGVAGLPGAKGEVGADGAQGIPGP
                     PGREGAAGSPGPKGEKGMPGEKGNPGKDGVGRPGLPGPPGPPGPVIYVSSEDKAIVST
                     PGPEGKPGYAGFPGPAGPKGDLGSKGEQGLPGPKGEKGEPGTIFSPDGRALGHPQKGA
                     KGEPGFRGPPGPYGRPGHKGEIGFPGRPGRPGTNGLKGEKGEPGDASLGFSMRGLPGP
                     PGPPGPPGPPGMPIYDSNAFVESGRPGLPGQQGVQGPSGPKGDKGEVGPPGPPGQFPI
                     DLFHLEAEMKGDKGDRGDAGQKGERGEPGAPGGGFFSSSVPGPPGPPGYPGIPGPKGE
                     SIRGPPGPPGPQGPPGIGYEGRQGPPGPPGPPGPPSFPGPHRQTVSVPGPPGPPGPPG
                     PPGAMGASAGQVRIWATYQTMLDKIREVPEGWLIFVAEREELYVRVRNGFRKVLLEAR
                     TALPRGTGNEVAALQPPLVQLHEGSPYTRREYSYSTARPWRADDILANPPRLPDRQPY
                     PGVPHHHSSYVHLPPARPTLSLAHTHQDFQPVLHLVALNTPLSGGMRGIRGADFQCFQ
                     QARAVGLSGTFRAFLSSRLQDLYSIVRRADRGSVPIVNLKDEVLSPSWDSLFSGSQGQ
                     LQPGARIFSFDGRDVLRHPAWPQKSVWHGSDPSGRRLMESYCETWRTETTGATGQASS
                     LLSGRLLEQKAASCHNSYIVLCIENSFMTSFSK"
     exon            728..1272
                     /gene="Col18a1"
                     /inference="alignment:Splign:2.1.0"
     exon            1273..1359
                     /gene="Col18a1"
                     /inference="alignment:Splign:2.1.0"
     exon            1360..1413
                     /gene="Col18a1"
                     /inference="alignment:Splign:2.1.0"
     exon            1414..1543
                     /gene="Col18a1"
                     /inference="alignment:Splign:2.1.0"
     exon            1544..1620
                     /gene="Col18a1"
                     /inference="alignment:Splign:2.1.0"
     exon            1621..1806
                     /gene="Col18a1"
                     /inference="alignment:Splign:2.1.0"
     exon            1807..1833
                     /gene="Col18a1"
                     /inference="alignment:Splign:2.1.0"
     exon            1834..1896
                     /gene="Col18a1"
                     /inference="alignment:Splign:2.1.0"
     exon            1897..1974
                     /gene="Col18a1"
                     /inference="alignment:Splign:2.1.0"
     exon            1975..2028
                     /gene="Col18a1"
                     /inference="alignment:Splign:2.1.0"
     exon            2029..2187
                     /gene="Col18a1"
                     /inference="alignment:Splign:2.1.0"
     exon            2188..2250
                     /gene="Col18a1"
                     /inference="alignment:Splign:2.1.0"
     exon            2251..2277
                     /gene="Col18a1"
                     /inference="alignment:Splign:2.1.0"
     exon            2278..2409
                     /gene="Col18a1"
                     /inference="alignment:Splign:2.1.0"
     exon            2410..2472
                     /gene="Col18a1"
                     /inference="alignment:Splign:2.1.0"
     exon            2473..2499
                     /gene="Col18a1"
                     /inference="alignment:Splign:2.1.0"
     exon            2500..2535
                     /gene="Col18a1"
                     /inference="alignment:Splign:2.1.0"
     exon            2536..2607
                     /gene="Col18a1"
                     /inference="alignment:Splign:2.1.0"
     exon            2608..2643
                     /gene="Col18a1"
                     /inference="alignment:Splign:2.1.0"
     exon            2644..2733
                     /gene="Col18a1"
                     /inference="alignment:Splign:2.1.0"
     exon            2734..2763
                     /gene="Col18a1"
                     /inference="alignment:Splign:2.1.0"
     exon            2764..2790
                     /gene="Col18a1"
                     /inference="alignment:Splign:2.1.0"
     exon            2791..2853
                     /gene="Col18a1"
                     /inference="alignment:Splign:2.1.0"
     exon            2854..2928
                     /gene="Col18a1"
                     /inference="alignment:Splign:2.1.0"
     exon            2929..2955
                     /gene="Col18a1"
                     /inference="alignment:Splign:2.1.0"
     exon            2956..3009
                     /gene="Col18a1"
                     /inference="alignment:Splign:2.1.0"
     exon            3010..3084
                     /gene="Col18a1"
                     /inference="alignment:Splign:2.1.0"
     exon            3085..3153
                     /gene="Col18a1"
                     /inference="alignment:Splign:2.1.0"
     exon            3154..3196
                     /gene="Col18a1"
                     /inference="alignment:Splign:2.1.0"
     exon            3197..3259
                     /gene="Col18a1"
                     /inference="alignment:Splign:2.1.0"
     exon            3260..3303
                     /gene="Col18a1"
                     /inference="alignment:Splign:2.1.0"
     exon            3304..3432
                     /gene="Col18a1"
                     /inference="alignment:Splign:2.1.0"
     exon            3433..3577
                     /gene="Col18a1"
                     /inference="alignment:Splign:2.1.0"
     exon            3578..3651
                     /gene="Col18a1"
                     /inference="alignment:Splign:2.1.0"
     exon            3652..3783
                     /gene="Col18a1"
                     /inference="alignment:Splign:2.1.0"
     exon            3784..3816
                     /gene="Col18a1"
                     /inference="alignment:Splign:2.1.0"
     exon            3817..4065
                     /gene="Col18a1"
                     /inference="alignment:Splign:2.1.0"
     exon            4066..4263
                     /gene="Col18a1"
                     /inference="alignment:Splign:2.1.0"
     exon            4264..4379
                     /gene="Col18a1"
                     /inference="alignment:Splign:2.1.0"
     exon            4380..5608
                     /gene="Col18a1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4728..4733
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Col18a1"
                     /note="hexamer: AATAAA"
     polyA_site      4749
                     /gene="Col18a1"
     regulatory      5585..5590
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Col18a1"
                     /note="hexamer: AATAAA"
     polyA_site      5608
                     /gene="Col18a1"
                     /note="major polyA site"
ORIGIN      
        1 actgccctga tggctcccga ccccagcaga cgcctctgcc tgctgctgct gttgctgctc
       61 tcctgccgcc ttgtgcctgc cagcgctgat gggaattccc tgagtcccct gaatcccctg
      121 gtgtggctct ggccgcccaa gacctctgat tctttggaag gcccggtctc gaaaccccag
      181 aacagttcac ctgtgcagtc cacagagaac cccaccacgc acgtggtccc ccaggatggc
      241 ctcacagaac aacagacgac tcctgccagc tccgagctgc ccccggagga agaggaggag
      301 gaggatcaga aagctggcca gggtggcagc cctgcaaccc ctgctgtccc cattcccctg
      361 gtggcccctg ctgcgagccc agacatgaag gaggagaatg ttgcgggcgt tggagccaag
      421 attctgaatg tggctcaggg cattcggagc ttcgtccagc tgtgggatga ggactcgact
      481 attgggcact ctgctgggac tgaggttcca gattcttcaa tccctacagt cctcccctct
      541 cctgccgagc tctccagtgc tccccagggg agtaagacca ctctctggct aagcagtgcc
      601 attccaagtt ctccagatgc tcagacaact gaggctggca cgctggctgt gcccacccag
      661 ctgcctccct tccagagcaa cctccaggca ccactgggaa gaccctcagc cccaccagac
      721 ttcccagaga atgttgctga ggaggtgggg ctgctgcagc tccttggaga ccccctacct
      781 gagaagatct cacaaatcga tgaccctcac gtcgggccgg cctacatctt tggaccagac
      841 tccaacagtg gccaggtggc ccagtatcat ttcccaaaac tcttcttccg ggacttttcg
      901 ctgctgtttc atgtccggcc agccacagag gcagcagggg tgctatttgc catcacagat
      961 gctgcccagg tggtagtctc actgggcgtg aagctctcag aggtccgaga tggacagcaa
     1021 aacatctcat tgctctacac ggagcctggg gccagccaga cccagacggg agccagcttc
     1081 cgcctacctg catttgttgg gcagtggaca cacttcgcgc tcagcgtcga cggaggctct
     1141 gtggctctct acgtagactg tgaagaattc cagagggtgc catttgctcg ggcctcgcag
     1201 ggactggagc tagagcgtgg cgctggcctc tttgtgggtc aggctggaac agcagaccct
     1261 gacaagttcc aggggatgat ctcagagctg aaggtacgca aaaccccccg ggtgagccct
     1321 gtgcactgtc tggatgaaga agatgatgat gaagaccggg catctggaga ttttggaagt
     1381 ggctttgaag aaagcagcaa gtcacacaag gaggatacat ctctactacc tgggctccct
     1441 cagccacctc ctgtcacttc cccacccctg gctggaggca gcaccacaga agatcctaga
     1501 acagaagaaa cggaggaaga cgccgcggta gattctatag gagctgagac ccttcctggc
     1561 acaggttcaa gcggtgcatg ggatgaggct atccagaacc ccggaagggg cttgataaag
     1621 ggaggtatga aaggacaaaa gggagaacca ggtgcccagg gcccacctgg cccagctggg
     1681 ccccagggtc ctgccggtcc agtggtccag agccccaact cacaacctgt ccctggagca
     1741 caaggacccc cgggacctca ggggccacca gggaaggatg gcactccagg aagggatggt
     1801 gaaccgggtg accctggtga agatgggaga ccgggtgaca ctggacctca aggctttcca
     1861 gggaccccag gagatgtggg ccctaagggc gagaagggag atcctggtat tgggccccga
     1921 ggacctccag ggcctccagg gccaccagga ccctccttca gacaagacaa gctgaccttc
     1981 attgacatgg agggatccgg tttcagcgga gacatagaga gccttagagg cccacgaggc
     2041 ttccctggcc ccccggggcc ccctggtgtc ccaggacttc ctggtgagcc aggacgcttt
     2101 gggatcaatg gttcctatgc accaggacct gcaggccttc ctggtgtacc tgggaaggaa
     2161 ggaccccccg gttttccagg tcccccggga cctccaggtc ctccaggcaa agagggccca
     2221 ccaggagtgg ccggccagaa aggcagtgtt ggtgatgtgg gcatcccagg acccaagggg
     2281 agcaaaggag accttgggcc catcggtatg cctggcaagt ctggcttggc tggatcccct
     2341 gggccagttg gacccccagg acctccaggg cctccagggc caccaggacc aggatttgct
     2401 gctggattcg atgatatgga aggctctgga atacccctct ggacaacagc ccgaagctct
     2461 gatgggctgc agggacctcc cgggtcgccg ggactcaagg gggatcctgg agtggcaggc
     2521 ctacctggag ccaagggaga agttggagca gatggagccc agggcatccc tggtccccca
     2581 ggaagagaag gtgcagctgg atctccgggg ccaaaaggag agaaagggat gccgggagaa
     2641 aagggaaacc caggaaaaga tggagtgggc cggccgggcc tccctgggcc tccaggacct
     2701 ccagggcctg tgatctatgt gtcaagtgag gataaagcaa tagtgagcac gccaggacct
     2761 gagggcaagc cagggtacgc aggctttcct ggacctgctg gaccgaaggg tgacctgggt
     2821 tccaaaggcg agcagggtct tccggggccc aagggtgaga agggagagcc aggcactatc
     2881 tttagtcctg atggcagagc tctgggccat ccccagaagg gagccaaggg agagccaggc
     2941 tttcgaggac ccccgggtcc ttatggacga cctgggcaca agggtgaaat tggcttccct
     3001 ggacggccgg gtcgacctgg aacgaatggc ttaaagggag agaaaggaga gcctggagat
     3061 gccagccttg ggttcagcat gaggggattg cctggccccc ctgggcctcc aggaccccca
     3121 ggtcctcctg ggatgcccat ctatgacagc aatgcatttg tggagtctgg ccgacctgga
     3181 ctaccaggac agcagggtgt gcaggggcct tcaggaccaa agggtgacaa aggagaggtg
     3241 ggcccacctg ggccaccagg gcaattcccc attgacctct tccacctgga agcggaaatg
     3301 aagggggaca agggagaccg aggggatgct ggacagaaag gagagagggg agaacctggg
     3361 gctcctggtg gtggattctt cagctcaagt gtacctggcc cccccggccc acctggatac
     3421 cctggaattc cgggtccaaa gggagagagc atccgggggc cacctggccc tcctggcccg
     3481 cagggacctc ctggcattgg ctatgagggt cgccagggtc ccccaggacc tccgggacct
     3541 ccaggacctc cctccttccc tggccctcac agacagactg tcagtgttcc tggtcctccg
     3601 ggcccacctg gtcctccagg tcccccagga gccatgggtg cctctgctgg gcaggtgagg
     3661 atctgggcca cataccagac catgctggac aagatccggg aggtgccgga gggctggctc
     3721 atctttgtgg ccgagaggga agagctctat gtacgcgtta gaaatggctt ccggaaggtg
     3781 ctgctggagg cccggacagc cctcccgaga ggcacgggca atgaggtggc tgctttgcag
     3841 cccccattgg tccagcttca tgagggcagt ccatacaccc ggagggagta ctcctattcc
     3901 acggcacgac cctggcgagc agatgacatc ctggccaacc caccgcgcct gccagaccgc
     3961 cagccttacc ccggagttcc acatcaccac agttcctatg tgcacctgcc gccagcccgc
     4021 cccaccctct cacttgctca tactcatcag gactttcagc cagtgctcca cctggtggca
     4081 ctgaacaccc ccctgtctgg aggcatgcgt ggtatccgtg gagcagattt ccagtgcttc
     4141 cagcaagccc gagccgtggg gctgtcgggc accttccggg ctttcctgtc ctctaggctg
     4201 caggatctct atagcatcgt gcgccgtgct gaccgggggt ctgtgcccat cgtcaacctg
     4261 aaggacgagg tgctatctcc cagctgggac tccctgtttt ctggctccca gggtcaactg
     4321 caacccgggg cccgcatctt ttcttttgac ggcagagatg tcctgagaca cccagcctgg
     4381 ccgcagaaga gcgtatggca cggctcggac cccagtgggc ggaggctgat ggagagttac
     4441 tgtgagacat ggcgaactga aactactggg gctacaggtc aggcctcctc cctgctgtca
     4501 ggcaggctcc tggaacagaa agctgcgagc tgccacaaca gctacatcgt cctgtgcatt
     4561 gagaatagct tcatgacctc tttctccaaa tagggcctct gccagctagg gtggcagaca
     4621 gaggccatgc agaactttga cacagcgcag ggagcattca gtcagcaccc agggctctgg
     4681 ctgggataca atcctgtata gttcccattt ttatgtaatc ctcaagaaat aaaaggaagc
     4741 caaagagtat atttttctaa aacatttaca acagacttcc tgcccttcct ctcccacttg
     4801 aactggccag tctgtgtcac agtcccttct tcctccgtgc ttggtagagc ttcctggcga
     4861 agtcggacca cagctaggtc tccaccggag gcgatgccct acagttgctg cctcagacct
     4921 gcagactcta tctagcctgg gatctttctg tgcagatcgt tcatacaaca gtcacatgcc
     4981 atgtgctggg gacagcacag ggcaggcagt ccaaggctcc cttctcaccg cccagtggct
     5041 gtgccaggca tataggcggg cacctgctca tccaggtggt aactgggtct actcatcctt
     5101 gagcagtggg aaccagctct cctgactgcc ccactctcct gcagccacac tccctgccag
     5161 gggaggttcc caagctcagt aactcactgt gaccccagat ctgacccccc ctcccgtcac
     5221 catgggcata ggccaggaat gtggccagac ttagcctgcg tgctcatttt tccgagggcc
     5281 agacgtgaca tggggcccat cctcaggttt gtctctcaga ctggcctctc tggcgacccc
     5341 tgagatcctg ttcagagaac ccaaccccaa ccaagcccaa gctggacatc agcttgaagt
     5401 ccagaaatct cacagcagcc acatgaagca cttgtcctat gaagggactt gtgtgcccag
     5461 ggtcacaagt ggccaatcaa agccactgtg cctgcctcag gctctgctgt ccaaggattg
     5521 ggacaatcca gtgggtctgg ttctgtgaca atgtgtgcta tgatgccatt tcaaagaggc
     5581 accaaataaa aatgacattt tataccaa
//
