LOCUS       NM_009908               1704 bp    mRNA    linear   ROD 06-AUG-2024
DEFINITION  Mus musculus cytidine monophospho-N-acetylneuraminic acid
            synthetase (Cmas), transcript variant 1, mRNA.
ACCESSION   NM_009908
VERSION     NM_009908.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1704)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 1704)
  AUTHORS   Linder,A.T., Schmidt,M., Hitschfel,J., Abeln,M., Schneider,P.,
            Gerardy-Schahn,R., Munster-Kuhnel,A.K. and Nitschke,L.
  TITLE     Sialic acids on B cells are crucial for their survival and provide
            protection against apoptosis
  JOURNAL   Proc Natl Acad Sci U S A 119 (25), e2201129119 (2022)
   PUBMED   35696562
REFERENCE   3  (bases 1 to 1704)
  AUTHORS   Urbanek,K., Sutherland,D.M., Orchard,R.C., Wilen,C.B.,
            Knowlton,J.J., Aravamudhan,P., Taylor,G.M., Virgin,H.W. and
            Dermody,T.S.
  TITLE     Cytidine Monophosphate N-Acetylneuraminic Acid Synthetase and
            Solute Carrier Family 35 Member A1 Are Required for Reovirus
            Binding and Infection
  JOURNAL   J Virol 95 (2), e01571-20 (2020)
   PUBMED   33087464
  REMARK    GeneRIF: Cytidine Monophosphate N-Acetylneuraminic Acid Synthetase
            and Solute Carrier Family 35 Member A1 Are Required for Reovirus
            Binding and Infection.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 1704)
  AUTHORS   Niculovic,K.M., Blume,L., Wedekind,H., Kats,E., Albers,I.,
            Groos,S., Abeln,M., Schmitz,J., Beuke,E., Brasen,J.H., Melk,A.,
            Schiffer,M., Weinhold,B. and Munster-Kuhnel,A.K.
  TITLE     Podocyte-Specific Sialylation-Deficient Mice Serve as a Model for
            Human FSGS
  JOURNAL   J Am Soc Nephrol 30 (6), 1021-1035 (2019)
   PUBMED   31040189
REFERENCE   5  (bases 1 to 1704)
  AUTHORS   Cornelissen,L.A.M., Blanas,A., van der Horst,J.C., Kruijssen,L.,
            Zaal,A., O'Toole,T., Wiercx,L., van Kooyk,Y. and van Vliet,S.J.
  TITLE     Disruption of sialic acid metabolism drives tumor growth by
            augmenting CD8+ T cell apoptosis
  JOURNAL   Int J Cancer 144 (9), 2290-2302 (2019)
   PUBMED   30578646
  REMARK    GeneRIF: CRISPR/Cas9-mediated knock out of the CMAS gene, encoding
            a key enzyme in the sialylation pathway, in the mouse colorectal
            cancer MC38 cell line completely abrogated cell surface expression
            of sialic acids (MC38-Sia(null) ) and, unexpectedly, significantly
            increased in vivo tumor growth compared to the control MC38-MOCK
            cells.
REFERENCE   6  (bases 1 to 1704)
  AUTHORS   Fujita,A., Sato,C. and Kitajima,K.
  TITLE     Identification of the nuclear export signals that regulate the
            intracellular localization of the mouse CMP-sialic acid synthetase
  JOURNAL   Biochem Biophys Res Commun 355 (1), 174-180 (2007)
   PUBMED   17292865
REFERENCE   7  (bases 1 to 1704)
  AUTHORS   Krapp,S., Munster-Kuhnel,A.K., Kaiser,J.T., Huber,R., Tiralongo,J.,
            Gerardy-Schahn,R. and Jacob,U.
  TITLE     The crystal structure of murine CMP-5-N-acetylneuraminic acid
            synthetase
  JOURNAL   J Mol Biol 334 (4), 625-637 (2003)
   PUBMED   14636592
  REMARK    GeneRIF: results describe the crystal structure of the N-terminal
            catalytically active domain of the murine 5-N-acetylneuraminic acid
            synthetase in complex with the reaction product
REFERENCE   8  (bases 1 to 1704)
  AUTHORS   Munster,A.K., Weinhold,B., Gotza,B., Muhlenhoff,M., Frosch,M. and
            Gerardy-Schahn,R.
  TITLE     Nuclear localization signal of murine CMP-Neu5Ac synthetase
            includes residues required for both nuclear targeting and enzymatic
            activity
  JOURNAL   J Biol Chem 277 (22), 19688-19696 (2002)
   PUBMED   11893746
  REMARK    GeneRIF: Nuclear localization signal of murine CMP-Neu5Ac
            synthetase includes residues required for both nuclear targeting
            and enzymatic activity
REFERENCE   9  (bases 1 to 1704)
  AUTHORS   Lawrence,S.M., Huddleston,K.A., Tomiya,N., Nguyen,N., Lee,Y.C.,
            Vann,W.F., Coleman,T.A. and Betenbaugh,M.J.
  TITLE     Cloning and expression of human sialic acid pathway genes to
            generate CMP-sialic acids in insect cells
  JOURNAL   Glycoconj J 18 (3), 205-213 (2001)
   PUBMED   11602804
REFERENCE   10 (bases 1 to 1704)
  AUTHORS   Munster,A.K., Eckhardt,M., Potvin,B., Muhlenhoff,M., Stanley,P. and
            Gerardy-Schahn,R.
  TITLE     Mammalian cytidine 5'-monophosphate N-acetylneuraminic acid
            synthetase: a nuclear protein with evolutionarily conserved
            structural motifs
  JOURNAL   Proc Natl Acad Sci U S A 95 (16), 9140-9145 (1998)
   PUBMED   9689047
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC164104.5.
            
            On Jul 20, 2022 this sequence version replaced NM_009908.2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AJ006215.1, BC063776.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849377
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-285               AC164104.5         193490-193774       c
            286-428             AC164104.5         186087-186229       c
            429-584             AC164104.5         185807-185962       c
            585-718             AC164104.5         182579-182712       c
            719-813             AC164104.5         179899-179993       c
            814-985             AC164104.5         179179-179350       c
            986-1139            AC164104.5         178248-178401       c
            1140-1704           AC164104.5         174802-175366       c
FEATURES             Location/Qualifiers
     source          1..1704
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="6"
                     /map="6 74.66 cM"
     gene            1..1704
                     /gene="Cmas"
                     /gene_synonym="CMPNeu5Ac; D6Bwg0250e"
                     /note="cytidine monophospho-N-acetylneuraminic acid
                     synthetase"
                     /db_xref="GeneID:12764"
                     /db_xref="MGI:MGI:1337124"
     exon            1..285
                     /gene="Cmas"
                     /gene_synonym="CMPNeu5Ac; D6Bwg0250e"
                     /inference="alignment:Splign:2.1.0"
     exon            286..428
                     /gene="Cmas"
                     /gene_synonym="CMPNeu5Ac; D6Bwg0250e"
                     /inference="alignment:Splign:2.1.0"
     exon            429..584
                     /gene="Cmas"
                     /gene_synonym="CMPNeu5Ac; D6Bwg0250e"
                     /inference="alignment:Splign:2.1.0"
     exon            585..718
                     /gene="Cmas"
                     /gene_synonym="CMPNeu5Ac; D6Bwg0250e"
                     /inference="alignment:Splign:2.1.0"
     exon            719..813
                     /gene="Cmas"
                     /gene_synonym="CMPNeu5Ac; D6Bwg0250e"
                     /inference="alignment:Splign:2.1.0"
     exon            814..985
                     /gene="Cmas"
                     /gene_synonym="CMPNeu5Ac; D6Bwg0250e"
                     /inference="alignment:Splign:2.1.0"
     exon            986..1139
                     /gene="Cmas"
                     /gene_synonym="CMPNeu5Ac; D6Bwg0250e"
                     /inference="alignment:Splign:2.1.0"
     exon            1140..1704
                     /gene="Cmas"
                     /gene_synonym="CMPNeu5Ac; D6Bwg0250e"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1678..1683
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Cmas"
                     /gene_synonym="CMPNeu5Ac; D6Bwg0250e"
                     /note="hexamer: AATAAA"
     polyA_site      1704
                     /gene="Cmas"
                     /gene_synonym="CMPNeu5Ac; D6Bwg0250e"
                     /note="major polyA site"
ORIGIN      
        1 ggagacgttg gagcggcggc gtgggaggaa gatggacgcg ctggagaagg gggccgccac
       61 gtcggggccc gccccgcgtg gacggccgtc ccggggccgg cccccgaagc tgcagcgcag
      121 ccggggcgcg gggcgcggcc tagagaagcc gccgcacctg gcagcgctgg tgctggcccg
      181 cggcggcagc aaaggcatcc cactgaagaa catcaagcgc ctggcggggg ttccgctcat
      241 tggctgggtc ctgcgcgccg ccctggatgc gggggtcttc cagagtgtgt gggtttcaac
      301 agaccatgat gaaattgaga atgtggccaa acagtttggt gcacaggtcc atcgaagaag
      361 ttctgaaacg tccaaagaca gctctacctc actagacgcc attgtagaat tcctgaatta
      421 tcacaatgag gttgacattg tggggaatat ccaagccaca tctccatgtt tacatcccac
      481 tgacctccag aaagttgcag aaatgatccg agaagaagga tatgactctg tcttctccgt
      541 tgtgaggcgc catcagtttc gatggagtga aattcagaaa ggagttcgtg aagtgactga
      601 gcctctgaac ttgaatccag cgaaacggcc tcgtcgacaa gactgggatg gagagttata
      661 tgagaacggc tcattttatt ttgctaaaag acatttgata gagatgggtt acttacaggg
      721 tgggaaaatg gcatattatg aaatgcgagc tgagcacagt gtggatatcg acgtggacat
      781 cgattggccg atcgcagagc aaagagttct gagatttggc tattttggaa aagagaagct
      841 gaaggagata aagcttttgg tttgtaatat tgatggatgt ctcaccaatg gccacattta
      901 tgtatcagga gaccaaaaag aaataatatc ttatgatgta aaagacgcta ttggcataag
      961 tttattaaag aaaagcggta ttgaggtgag gctcatctca gaacgggcct gctccaagca
     1021 gacgctctct gccctaaagc tggactgtaa aacagaagtc agtgtgtccg ataagctggc
     1081 caccgtggat gagtggagga aggagatggg cctgtgctgg aaagaagtgg cctatctcgg
     1141 caatgaagtg tctgatgaag aatgcctcaa gagagtgggc ctgagcgctg ttcctgccga
     1201 cgcctgctcc ggggcccaga aggctgtggg gtacatctgc aaatgcagcg gtggccgggg
     1261 agccatccgc gagtttgcag agcacatttt cctactgata gaaaaagtta ataactcatg
     1321 ccaaaaatag aaaatgttgt agtgtggggg aaagggagat tgaagccttt tccagccatt
     1381 ttgcattgtt ttgatcaagt cccgaagtgt aatctgcagg tgtgtggtct gatccgtgag
     1441 ctagttctct attctcaaag ctgagttttc tccaatcatg gttcctgaac ccatgacata
     1501 atcatgtctg ttttcctctt gatgcgaggt aattaccgag ggattgacct agtcttgctc
     1561 cgacctcata aacaatccac tctgtattgt gccctgtcaa actagtgtgt gtggtgttaa
     1621 tgatgctggg attttattta ttgtaaagca atgagtctgg taagacaccc ctgtgttaat
     1681 aaagttaccg ttcacagact tgaa
//
