LOCUS       NP_034038                432 aa            linear   ROD 06-AUG-2024
DEFINITION  N-acylneuraminate cytidylyltransferase isoform 1 [Mus musculus].
ACCESSION   NP_034038
VERSION     NP_034038.2
DBSOURCE    REFSEQ: accession NM_009908.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (residues 1 to 432)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (residues 1 to 432)
  AUTHORS   Linder,A.T., Schmidt,M., Hitschfel,J., Abeln,M., Schneider,P.,
            Gerardy-Schahn,R., Munster-Kuhnel,A.K. and Nitschke,L.
  TITLE     Sialic acids on B cells are crucial for their survival and provide
            protection against apoptosis
  JOURNAL   Proc Natl Acad Sci U S A 119 (25), e2201129119 (2022)
   PUBMED   35696562
REFERENCE   3  (residues 1 to 432)
  AUTHORS   Urbanek,K., Sutherland,D.M., Orchard,R.C., Wilen,C.B.,
            Knowlton,J.J., Aravamudhan,P., Taylor,G.M., Virgin,H.W. and
            Dermody,T.S.
  TITLE     Cytidine Monophosphate N-Acetylneuraminic Acid Synthetase and
            Solute Carrier Family 35 Member A1 Are Required for Reovirus
            Binding and Infection
  JOURNAL   J Virol 95 (2), e01571-20 (2020)
   PUBMED   33087464
  REMARK    GeneRIF: Cytidine Monophosphate N-Acetylneuraminic Acid Synthetase
            and Solute Carrier Family 35 Member A1 Are Required for Reovirus
            Binding and Infection.
            Publication Status: Online-Only
REFERENCE   4  (residues 1 to 432)
  AUTHORS   Niculovic,K.M., Blume,L., Wedekind,H., Kats,E., Albers,I.,
            Groos,S., Abeln,M., Schmitz,J., Beuke,E., Brasen,J.H., Melk,A.,
            Schiffer,M., Weinhold,B. and Munster-Kuhnel,A.K.
  TITLE     Podocyte-Specific Sialylation-Deficient Mice Serve as a Model for
            Human FSGS
  JOURNAL   J Am Soc Nephrol 30 (6), 1021-1035 (2019)
   PUBMED   31040189
REFERENCE   5  (residues 1 to 432)
  AUTHORS   Cornelissen,L.A.M., Blanas,A., van der Horst,J.C., Kruijssen,L.,
            Zaal,A., O'Toole,T., Wiercx,L., van Kooyk,Y. and van Vliet,S.J.
  TITLE     Disruption of sialic acid metabolism drives tumor growth by
            augmenting CD8+ T cell apoptosis
  JOURNAL   Int J Cancer 144 (9), 2290-2302 (2019)
   PUBMED   30578646
  REMARK    GeneRIF: CRISPR/Cas9-mediated knock out of the CMAS gene, encoding
            a key enzyme in the sialylation pathway, in the mouse colorectal
            cancer MC38 cell line completely abrogated cell surface expression
            of sialic acids (MC38-Sia(null) ) and, unexpectedly, significantly
            increased in vivo tumor growth compared to the control MC38-MOCK
            cells.
REFERENCE   6  (residues 1 to 432)
  AUTHORS   Fujita,A., Sato,C. and Kitajima,K.
  TITLE     Identification of the nuclear export signals that regulate the
            intracellular localization of the mouse CMP-sialic acid synthetase
  JOURNAL   Biochem Biophys Res Commun 355 (1), 174-180 (2007)
   PUBMED   17292865
REFERENCE   7  (residues 1 to 432)
  AUTHORS   Krapp,S., Munster-Kuhnel,A.K., Kaiser,J.T., Huber,R., Tiralongo,J.,
            Gerardy-Schahn,R. and Jacob,U.
  TITLE     The crystal structure of murine CMP-5-N-acetylneuraminic acid
            synthetase
  JOURNAL   J Mol Biol 334 (4), 625-637 (2003)
   PUBMED   14636592
  REMARK    GeneRIF: results describe the crystal structure of the N-terminal
            catalytically active domain of the murine 5-N-acetylneuraminic acid
            synthetase in complex with the reaction product
REFERENCE   8  (residues 1 to 432)
  AUTHORS   Munster,A.K., Weinhold,B., Gotza,B., Muhlenhoff,M., Frosch,M. and
            Gerardy-Schahn,R.
  TITLE     Nuclear localization signal of murine CMP-Neu5Ac synthetase
            includes residues required for both nuclear targeting and enzymatic
            activity
  JOURNAL   J Biol Chem 277 (22), 19688-19696 (2002)
   PUBMED   11893746
  REMARK    GeneRIF: Nuclear localization signal of murine CMP-Neu5Ac
            synthetase includes residues required for both nuclear targeting
            and enzymatic activity
REFERENCE   9  (residues 1 to 432)
  AUTHORS   Lawrence,S.M., Huddleston,K.A., Tomiya,N., Nguyen,N., Lee,Y.C.,
            Vann,W.F., Coleman,T.A. and Betenbaugh,M.J.
  TITLE     Cloning and expression of human sialic acid pathway genes to
            generate CMP-sialic acids in insect cells
  JOURNAL   Glycoconj J 18 (3), 205-213 (2001)
   PUBMED   11602804
REFERENCE   10 (residues 1 to 432)
  AUTHORS   Munster,A.K., Eckhardt,M., Potvin,B., Muhlenhoff,M., Stanley,P. and
            Gerardy-Schahn,R.
  TITLE     Mammalian cytidine 5'-monophosphate N-acetylneuraminic acid
            synthetase: a nuclear protein with evolutionarily conserved
            structural motifs
  JOURNAL   Proc Natl Acad Sci U S A 95 (16), 9140-9145 (1998)
   PUBMED   9689047
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC164104.5.
            
            On Jul 2, 2009 this sequence version replaced NP_034038.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AJ006215.1, BC063776.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849377
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..432
                     /organism="Mus musculus"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="6"
                     /map="6 74.66 cM"
     Protein         1..432
                     /product="N-acylneuraminate cytidylyltransferase isoform
                     1"
                     /EC_number="2.7.7.43"
                     /note="CMP-N-acetylneuraminic acid synthetase;
                     N-acylneuraminate cytidylyltransferase; CMP-NeuNAc
                     synthetase; CMP-NeuNAc synthase; CMP-N-acetylneuraminic
                     acid synthase; CMP-Neu5Ac synthase; CMP-sialic acid
                     synthetase"
                     /calculated_mol_wt=47899
     Region          1..38
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (Q99KK2.2)"
     Site            1
                     /site_type="acetylation"
                     /note="N-acetylmethionine.
                     /evidence=ECO:0000250|UniProtKB:Q8NFW8; propagated from
                     UniProtKB/Swiss-Prot (Q99KK2.2)"
     Region          15..31
                     /region_name="BC1 motif"
                     /note="propagated from UniProtKB/Swiss-Prot (Q99KK2.2)"
     Site            35
                     /site_type="methylation"
                     /note="Omega-N-methylarginine.
                     /evidence=ECO:0007744|PubMed:24129315; propagated from
                     UniProtKB/Swiss-Prot (Q99KK2.2)"
     Region          42..257
                     /region_name="CMP-NeuAc_Synthase"
                     /note="CMP-NeuAc_Synthase activates N-acetylneuraminic
                     acid by adding CMP moiety; cd02513"
                     /db_xref="CDD:133006"
     Site            order(48..50,54,60,109,113,120,141,143,211,216,247)
                     /site_type="active"
                     /note="ligand binding site [active]"
                     /db_xref="CDD:133006"
     Site            50
                     /site_type="methylation"
                     /note="Omega-N-methylarginine.
                     /evidence=ECO:0007744|PubMed:24129315; propagated from
                     UniProtKB/Swiss-Prot (Q99KK2.2)"
     Site            order(63,65..66,168,172..173,208..211,216,233,235,247,
                     250..251,254,257)
                     /site_type="active"
                     /note="tetramer interface [active]"
                     /db_xref="CDD:133006"
     Region          198..204
                     /region_name="BC2 motif"
                     /note="propagated from UniProtKB/Swiss-Prot (Q99KK2.2)"
     Region          267..274
                     /region_name="BC3 motif"
                     /note="propagated from UniProtKB/Swiss-Prot (Q99KK2.2)"
     Region          273..417
                     /region_name="HAD_KDO-like"
                     /note="haloacid dehalogenase-like (HAD) hydrolase, similar
                     to Escherichia coli 3-deoxy-D-manno-octulosonate
                     8-phosphate (KDO 8-P) phosphatase KdsC, and rainbow trout
                     N-acylneuraminate cytidylyltransferase; cd01630"
                     /db_xref="CDD:319769"
     Site            order(279..283,323..324,348,370..371,374..375)
                     /site_type="active"
                     /db_xref="CDD:319769"
     Site            order(281,286..293,295,297..304,307,311,325,329..330,
                     333..334,337,346,371..374,376..377,390,394,409,413,417)
                     /site_type="other"
                     /note="tetramer interface [polypeptide binding]"
                     /db_xref="CDD:319769"
     CDS             1..432
                     /gene="Cmas"
                     /gene_synonym="CMPNeu5Ac; D6Bwg0250e"
                     /coded_by="NM_009908.3:32..1330"
                     /note="isoform 1 is encoded by transcript variant 1"
                     /db_xref="CCDS:CCDS20687.1"
                     /db_xref="GeneID:12764"
                     /db_xref="MGI:MGI:1337124"
ORIGIN      
        1 mdalekgaat sgpaprgrps rgrppklqrs rgagrglekp phlaalvlar ggskgiplkn
       61 ikrlagvpli gwvlraalda gvfqsvwvst dhdeienvak qfgaqvhrrs setskdssts
      121 ldaiveflny hnevdivgni qatspclhpt dlqkvaemir eegydsvfsv vrrhqfrwse
      181 iqkgvrevte plnlnpakrp rrqdwdgely engsfyfakr hliemgylqg gkmayyemra
      241 ehsvdidvdi dwpiaeqrvl rfgyfgkekl keikllvcni dgcltnghiy vsgdqkeiis
      301 ydvkdaigis llkksgievr liseracskq tlsalkldck tevsvsdkla tvdewrkemg
      361 lcwkevaylg nevsdeeclk rvglsavpad acsgaqkavg yickcsggrg airefaehif
      421 lliekvnnsc qk
//
