LOCUS       NM_001409575            1561 bp    mRNA    linear   ROD 06-AUG-2024
DEFINITION  Mus musculus cytidine monophospho-N-acetylneuraminic acid
            synthetase (Cmas), transcript variant 2, mRNA.
ACCESSION   NM_001409575
VERSION     NM_001409575.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1561)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 1561)
  AUTHORS   Linder,A.T., Schmidt,M., Hitschfel,J., Abeln,M., Schneider,P.,
            Gerardy-Schahn,R., Munster-Kuhnel,A.K. and Nitschke,L.
  TITLE     Sialic acids on B cells are crucial for their survival and provide
            protection against apoptosis
  JOURNAL   Proc Natl Acad Sci U S A 119 (25), e2201129119 (2022)
   PUBMED   35696562
REFERENCE   3  (bases 1 to 1561)
  AUTHORS   Urbanek,K., Sutherland,D.M., Orchard,R.C., Wilen,C.B.,
            Knowlton,J.J., Aravamudhan,P., Taylor,G.M., Virgin,H.W. and
            Dermody,T.S.
  TITLE     Cytidine Monophosphate N-Acetylneuraminic Acid Synthetase and
            Solute Carrier Family 35 Member A1 Are Required for Reovirus
            Binding and Infection
  JOURNAL   J Virol 95 (2), e01571-20 (2020)
   PUBMED   33087464
  REMARK    GeneRIF: Cytidine Monophosphate N-Acetylneuraminic Acid Synthetase
            and Solute Carrier Family 35 Member A1 Are Required for Reovirus
            Binding and Infection.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 1561)
  AUTHORS   Niculovic,K.M., Blume,L., Wedekind,H., Kats,E., Albers,I.,
            Groos,S., Abeln,M., Schmitz,J., Beuke,E., Brasen,J.H., Melk,A.,
            Schiffer,M., Weinhold,B. and Munster-Kuhnel,A.K.
  TITLE     Podocyte-Specific Sialylation-Deficient Mice Serve as a Model for
            Human FSGS
  JOURNAL   J Am Soc Nephrol 30 (6), 1021-1035 (2019)
   PUBMED   31040189
REFERENCE   5  (bases 1 to 1561)
  AUTHORS   Cornelissen,L.A.M., Blanas,A., van der Horst,J.C., Kruijssen,L.,
            Zaal,A., O'Toole,T., Wiercx,L., van Kooyk,Y. and van Vliet,S.J.
  TITLE     Disruption of sialic acid metabolism drives tumor growth by
            augmenting CD8+ T cell apoptosis
  JOURNAL   Int J Cancer 144 (9), 2290-2302 (2019)
   PUBMED   30578646
  REMARK    GeneRIF: CRISPR/Cas9-mediated knock out of the CMAS gene, encoding
            a key enzyme in the sialylation pathway, in the mouse colorectal
            cancer MC38 cell line completely abrogated cell surface expression
            of sialic acids (MC38-Sia(null) ) and, unexpectedly, significantly
            increased in vivo tumor growth compared to the control MC38-MOCK
            cells.
REFERENCE   6  (bases 1 to 1561)
  AUTHORS   Fujita,A., Sato,C. and Kitajima,K.
  TITLE     Identification of the nuclear export signals that regulate the
            intracellular localization of the mouse CMP-sialic acid synthetase
  JOURNAL   Biochem Biophys Res Commun 355 (1), 174-180 (2007)
   PUBMED   17292865
REFERENCE   7  (bases 1 to 1561)
  AUTHORS   Krapp,S., Munster-Kuhnel,A.K., Kaiser,J.T., Huber,R., Tiralongo,J.,
            Gerardy-Schahn,R. and Jacob,U.
  TITLE     The crystal structure of murine CMP-5-N-acetylneuraminic acid
            synthetase
  JOURNAL   J Mol Biol 334 (4), 625-637 (2003)
   PUBMED   14636592
  REMARK    GeneRIF: results describe the crystal structure of the N-terminal
            catalytically active domain of the murine 5-N-acetylneuraminic acid
            synthetase in complex with the reaction product
REFERENCE   8  (bases 1 to 1561)
  AUTHORS   Munster,A.K., Weinhold,B., Gotza,B., Muhlenhoff,M., Frosch,M. and
            Gerardy-Schahn,R.
  TITLE     Nuclear localization signal of murine CMP-Neu5Ac synthetase
            includes residues required for both nuclear targeting and enzymatic
            activity
  JOURNAL   J Biol Chem 277 (22), 19688-19696 (2002)
   PUBMED   11893746
  REMARK    GeneRIF: Nuclear localization signal of murine CMP-Neu5Ac
            synthetase includes residues required for both nuclear targeting
            and enzymatic activity
REFERENCE   9  (bases 1 to 1561)
  AUTHORS   Lawrence,S.M., Huddleston,K.A., Tomiya,N., Nguyen,N., Lee,Y.C.,
            Vann,W.F., Coleman,T.A. and Betenbaugh,M.J.
  TITLE     Cloning and expression of human sialic acid pathway genes to
            generate CMP-sialic acids in insect cells
  JOURNAL   Glycoconj J 18 (3), 205-213 (2001)
   PUBMED   11602804
REFERENCE   10 (bases 1 to 1561)
  AUTHORS   Munster,A.K., Eckhardt,M., Potvin,B., Muhlenhoff,M., Stanley,P. and
            Gerardy-Schahn,R.
  TITLE     Mammalian cytidine 5'-monophosphate N-acetylneuraminic acid
            synthetase: a nuclear protein with evolutionarily conserved
            structural motifs
  JOURNAL   Proc Natl Acad Sci U S A 95 (16), 9140-9145 (1998)
   PUBMED   9689047
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC164104.5.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR13422601.1750004.1,
                                           SRR13422592.88484.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849377
                                           [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-285               AC164104.5         193490-193774       c
            286-441             AC164104.5         185807-185962       c
            442-575             AC164104.5         182579-182712       c
            576-670             AC164104.5         179899-179993       c
            671-842             AC164104.5         179179-179350       c
            843-996             AC164104.5         178248-178401       c
            997-1561            AC164104.5         174802-175366       c
FEATURES             Location/Qualifiers
     source          1..1561
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="6"
                     /map="6 74.66 cM"
     gene            1..1561
                     /gene="Cmas"
                     /gene_synonym="CMPNeu5Ac; D6Bwg0250e"
                     /note="cytidine monophospho-N-acetylneuraminic acid
                     synthetase"
                     /db_xref="GeneID:12764"
                     /db_xref="MGI:MGI:1337124"
     exon            1..285
                     /gene="Cmas"
                     /gene_synonym="CMPNeu5Ac; D6Bwg0250e"
                     /inference="alignment:Splign:2.1.0"
     CDS             267..1187
                     /gene="Cmas"
                     /gene_synonym="CMPNeu5Ac; D6Bwg0250e"
                     /EC_number="2.7.7.43"
                     /note="isoform 2 is encoded by transcript variant 2;
                     CMP-N-acetylneuraminic acid synthetase; N-acylneuraminate
                     cytidylyltransferase; CMP-NeuNAc synthetase; CMP-NeuNAc
                     synthase; CMP-N-acetylneuraminic acid synthase; CMP-Neu5Ac
                     synthase; CMP-sialic acid synthetase"
                     /codon_start=1
                     /product="N-acylneuraminate cytidylyltransferase isoform
                     2"
                     /protein_id="NP_001396504.1"
                     /db_xref="GeneID:12764"
                     /db_xref="MGI:MGI:1337124"
                     /translation="MRGSSREVDIVGNIQATSPCLHPTDLQKVAEMIREEGYDSVFSV
                     VRRHQFRWSEIQKGVREVTEPLNLNPAKRPRRQDWDGELYENGSFYFAKRHLIEMGYL
                     QGGKMAYYEMRAEHSVDIDVDIDWPIAEQRVLRFGYFGKEKLKEIKLLVCNIDGCLTN
                     GHIYVSGDQKEIISYDVKDAIGISLLKKSGIEVRLISERACSKQTLSALKLDCKTEVS
                     VSDKLATVDEWRKEMGLCWKEVAYLGNEVSDEECLKRVGLSAVPADACSGAQKAVGYI
                     CKCSGGRGAIREFAEHIFLLIEKVNNSCQK"
     exon            286..441
                     /gene="Cmas"
                     /gene_synonym="CMPNeu5Ac; D6Bwg0250e"
                     /inference="alignment:Splign:2.1.0"
     exon            442..575
                     /gene="Cmas"
                     /gene_synonym="CMPNeu5Ac; D6Bwg0250e"
                     /inference="alignment:Splign:2.1.0"
     exon            576..670
                     /gene="Cmas"
                     /gene_synonym="CMPNeu5Ac; D6Bwg0250e"
                     /inference="alignment:Splign:2.1.0"
     exon            671..842
                     /gene="Cmas"
                     /gene_synonym="CMPNeu5Ac; D6Bwg0250e"
                     /inference="alignment:Splign:2.1.0"
     exon            843..996
                     /gene="Cmas"
                     /gene_synonym="CMPNeu5Ac; D6Bwg0250e"
                     /inference="alignment:Splign:2.1.0"
     exon            997..1561
                     /gene="Cmas"
                     /gene_synonym="CMPNeu5Ac; D6Bwg0250e"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1535..1540
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Cmas"
                     /gene_synonym="CMPNeu5Ac; D6Bwg0250e"
                     /note="hexamer: AATAAA"
     polyA_site      1561
                     /gene="Cmas"
                     /gene_synonym="CMPNeu5Ac; D6Bwg0250e"
                     /note="major polyA site"
ORIGIN      
        1 ggagacgttg gagcggcggc gtgggaggaa gatggacgcg ctggagaagg gggccgccac
       61 gtcggggccc gccccgcgtg gacggccgtc ccggggccgg cccccgaagc tgcagcgcag
      121 ccggggcgcg gggcgcggcc tagagaagcc gccgcacctg gcagcgctgg tgctggcccg
      181 cggcggcagc aaaggcatcc cactgaagaa catcaagcgc ctggcggggg ttccgctcat
      241 tggctgggtc ctgcgcgccg ccctggatgc gggggtcttc cagagaggtt gacattgtgg
      301 ggaatatcca agccacatct ccatgtttac atcccactga cctccagaaa gttgcagaaa
      361 tgatccgaga agaaggatat gactctgtct tctccgttgt gaggcgccat cagtttcgat
      421 ggagtgaaat tcagaaagga gttcgtgaag tgactgagcc tctgaacttg aatccagcga
      481 aacggcctcg tcgacaagac tgggatggag agttatatga gaacggctca ttttattttg
      541 ctaaaagaca tttgatagag atgggttact tacagggtgg gaaaatggca tattatgaaa
      601 tgcgagctga gcacagtgtg gatatcgacg tggacatcga ttggccgatc gcagagcaaa
      661 gagttctgag atttggctat tttggaaaag agaagctgaa ggagataaag cttttggttt
      721 gtaatattga tggatgtctc accaatggcc acatttatgt atcaggagac caaaaagaaa
      781 taatatctta tgatgtaaaa gacgctattg gcataagttt attaaagaaa agcggtattg
      841 aggtgaggct catctcagaa cgggcctgct ccaagcagac gctctctgcc ctaaagctgg
      901 actgtaaaac agaagtcagt gtgtccgata agctggccac cgtggatgag tggaggaagg
      961 agatgggcct gtgctggaaa gaagtggcct atctcggcaa tgaagtgtct gatgaagaat
     1021 gcctcaagag agtgggcctg agcgctgttc ctgccgacgc ctgctccggg gcccagaagg
     1081 ctgtggggta catctgcaaa tgcagcggtg gccggggagc catccgcgag tttgcagagc
     1141 acattttcct actgatagaa aaagttaata actcatgcca aaaatagaaa atgttgtagt
     1201 gtgggggaaa gggagattga agccttttcc agccattttg cattgttttg atcaagtccc
     1261 gaagtgtaat ctgcaggtgt gtggtctgat ccgtgagcta gttctctatt ctcaaagctg
     1321 agttttctcc aatcatggtt cctgaaccca tgacataatc atgtctgttt tcctcttgat
     1381 gcgaggtaat taccgaggga ttgacctagt cttgctccga cctcataaac aatccactct
     1441 gtattgtgcc ctgtcaaact agtgtgtgtg gtgttaatga tgctgggatt ttatttattg
     1501 taaagcaatg agtctggtaa gacacccctg tgttaataaa gttaccgttc acagacttga
     1561 a
//
