LOCUS       NM_029556               1257 bp    mRNA    linear   ROD 01-AUG-2024
DEFINITION  Mus musculus citrate lyase beta like (Clybl), mRNA; nuclear gene
            for mitochondrial product.
ACCESSION   NM_029556
VERSION     NM_029556.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1257)
  AUTHORS   Yu,Z., Li,X., Quan,Y., Chen,J., Liu,J., Zheng,N., Liu,S., Wang,Y.,
            Liu,W., Qiu,C., Wang,Y., Zheng,R. and Qin,J.
  TITLE     Itaconate alleviates diet-induced obesity via activation of brown
            adipocyte thermogenesis
  JOURNAL   Cell Rep 43 (5), 114142 (2024)
   PUBMED   38691458
REFERENCE   2  (bases 1 to 1257)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 1257)
  AUTHORS   Bedogni,F. and Hevner,R.F.
  TITLE     Cell-Type-Specific Gene Expression in Developing Mouse Neocortex:
            Intermediate Progenitors Implicated in Axon Development
  JOURNAL   Front Mol Neurosci 14, 686034 (2021)
   PUBMED   34321999
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 1257)
  AUTHORS   Shen,H., Campanello,G.C., Flicker,D., Grabarek,Z., Hu,J., Luo,C.,
            Banerjee,R. and Mootha,V.K.
  TITLE     The Human Knockout Gene CLYBL Connects Itaconate to Vitamin B12
  JOURNAL   Cell 171 (4), 771-782 (2017)
   PUBMED   29056341
REFERENCE   5  (bases 1 to 1257)
  AUTHORS   Koscielny,G., Yaikhom,G., Iyer,V., Meehan,T.F., Morgan,H.,
            Atienza-Herrero,J., Blake,A., Chen,C.K., Easty,R., Di Fenza,A.,
            Fiegel,T., Grifiths,M., Horne,A., Karp,N.A., Kurbatova,N.,
            Mason,J.C., Matthews,P., Oakley,D.J., Qazi,A., Regnart,J.,
            Retha,A., Santos,L.A., Sneddon,D.J., Warren,J., Westerberg,H.,
            Wilson,R.J., Melvin,D.G., Smedley,D., Brown,S.D., Flicek,P.,
            Skarnes,W.C., Mallon,A.M. and Parkinson,H.
  TITLE     The International Mouse Phenotyping Consortium Web Portal, a
            unified point of access for knockout mice and related phenotyping
            data
  JOURNAL   Nucleic Acids Res 42 (Database issue), D802-D809 (2014)
   PUBMED   24194600
REFERENCE   6  (bases 1 to 1257)
  AUTHORS   Lin,Q., Donahue,S.L., Moore-Jarrett,T., Cao,S., Osipovich,A.B. and
            Ruley,H.E.
  TITLE     Mutagenesis of diploid mammalian genes by gene entrapment
  JOURNAL   Nucleic Acids Res 34 (20), e139 (2006)
   PUBMED   17062627
REFERENCE   7  (bases 1 to 1257)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
REFERENCE   8  (bases 1 to 1257)
  AUTHORS   Morikawa,J., Nishimura,Y., Uchida,A. and Tanaka,T.
  TITLE     Molecular cloning of novel mouse and human putative citrate lyase
            beta-subunit
  JOURNAL   Biochem Biophys Res Commun 289 (5), 1282-1286 (2001)
   PUBMED   11741334
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC164315.2, AK009345.1 and CX202248.1.
            
            On Aug 20, 2009 this sequence version replaced NM_029556.2.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC023398.1, AK009345.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849375, SAMN00849380
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-13                AC164315.2         126689-126701
            14-1174             AK009345.1         2-1162
            1175-1257           CX202248.1         84-166
FEATURES             Location/Qualifiers
     source          1..1257
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="14"
                     /map="14 65.86 cM"
     gene            1..1257
                     /gene="Clybl"
                     /gene_synonym="0610033J05Rik; 2310014M14Rik; Clb"
                     /note="citrate lyase beta like"
                     /db_xref="GeneID:69634"
                     /db_xref="MGI:MGI:1916884"
     exon            1..88
                     /gene="Clybl"
                     /gene_synonym="0610033J05Rik; 2310014M14Rik; Clb"
                     /inference="alignment:Splign:2.1.0"
     CDS             33..1049
                     /gene="Clybl"
                     /gene_synonym="0610033J05Rik; 2310014M14Rik; Clb"
                     /EC_number="2.3.3.9"
                     /EC_number="4.1.3.25"
                     /EC_number="3.1.2.30"
                     /note="citrate lyase subunit beta-like protein,
                     mitochondrial; malate synthase; (3S)-malyl-CoA
                     thioesterase; beta-methylmalate synthase; citramalyl-CoA
                     lyase, mitochondrial"
                     /codon_start=1
                     /product="citramalyl-CoA lyase, mitochondrial precursor"
                     /protein_id="NP_083832.2"
                     /db_xref="CCDS:CCDS27346.1"
                     /db_xref="GeneID:69634"
                     /db_xref="MGI:MGI:1916884"
                     /translation="MALCVLRNTVRGAAALPRLKASHVVSVYKPRYSSLSNHKYVPRR
                     AVLYVPGNDEKKIRKIPSLKVDCAVLDCEDGVAENKKNEARLRIAKTLEDFDLGTTEK
                     CVRINSVSSGLAEVDLETFLQARVLPSSLMLPKVEGPEEIRWFSDKFSLHLKGRKLEQ
                     PMNLIPFVETAMGLLNFKAVCEETLKTGPQVGLCLDAVVFGGEDFRASIGATSNKDTQ
                     DILYARQKVVVTAKAFGLQAIDLVYIDFRDEDGLLRQSREAAAMGFTGKQVIHPNQIA
                     VVQEQFTPTPEKIQWAEELIAAFKEHQQLGKGAFTFRGSMIDMPLLKQAQNIVTLATS
                     IKEK"
     exon            89..275
                     /gene="Clybl"
                     /gene_synonym="0610033J05Rik; 2310014M14Rik; Clb"
                     /inference="alignment:Splign:2.1.0"
     exon            276..464
                     /gene="Clybl"
                     /gene_synonym="0610033J05Rik; 2310014M14Rik; Clb"
                     /inference="alignment:Splign:2.1.0"
     exon            465..566
                     /gene="Clybl"
                     /gene_synonym="0610033J05Rik; 2310014M14Rik; Clb"
                     /inference="alignment:Splign:2.1.0"
     exon            567..660
                     /gene="Clybl"
                     /gene_synonym="0610033J05Rik; 2310014M14Rik; Clb"
                     /inference="alignment:Splign:2.1.0"
     exon            661..828
                     /gene="Clybl"
                     /gene_synonym="0610033J05Rik; 2310014M14Rik; Clb"
                     /inference="alignment:Splign:2.1.0"
     exon            829..953
                     /gene="Clybl"
                     /gene_synonym="0610033J05Rik; 2310014M14Rik; Clb"
                     /inference="alignment:Splign:2.1.0"
     exon            954..1241
                     /gene="Clybl"
                     /gene_synonym="0610033J05Rik; 2310014M14Rik; Clb"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1215..1220
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Clybl"
                     /gene_synonym="0610033J05Rik; 2310014M14Rik; Clb"
                     /note="hexamer: AATAAA"
     polyA_site      1241
                     /gene="Clybl"
                     /gene_synonym="0610033J05Rik; 2310014M14Rik; Clb"
                     /note="major polyA site"
ORIGIN      
        1 gagggcgggg cgtaggcggg ccagtcggga agatggcgtt gtgcgtgctg cggaacacgg
       61 ttcgtggagc ggcggcgctg cccaggctga aggcatccca cgtggtcagt gtctacaagc
      121 ctagatacag ctccttgtcc aatcacaagt atgtcccccg gagagcagtg ctctatgtcc
      181 ctgggaatga cgaaaagaaa ataaggaaga tcccgtcctt gaaagtagat tgtgccgtgc
      241 tggactgcga ggatggtgtt gcagaaaaca aaaagaatga agctcgattg agaatagcaa
      301 aaactcttga agactttgac ctgggcacaa cggaaaagtg tgtgagaatc aactcggtgt
      361 ctagtggtct ggctgaagtc gacctggaga catttctaca ggccagagtt cttccctcta
      421 gtctgatgct accaaaggta gaaggtcctg aagaaatccg atggttttca gacaaatttt
      481 cactccactt aaaaggccga aaacttgaac aaccaatgaa tttaatcccc tttgtggaga
      541 ctgcaatggg tttgctcaat tttaaggcag tgtgtgaaga aacactaaag actggacctc
      601 aagtgggtct ttgcctagat gcagtcgttt ttggaggaga agattttcga gccagcatag
      661 gtgcaacaag caacaaagac acccaagaca tcctctatgc ccggcagaag gttgttgtca
      721 cggcaaaagc ctttggccta caagccatag atcttgtgta cattgacttc cgagatgaag
      781 acggacttct cagacagtca agggaagcag ccgccatggg cttcactggt aaacaggtga
      841 tccaccctaa ccagatcgca gtggtacagg aacagtttac tccaacccca gaaaaaattc
      901 agtgggctga agagctgatt gctgccttca aagaacacca gcagctggga aagggagcct
      961 ttactttccg aggaagtatg atcgacatgc cattactgaa gcaggcccag aacattgtta
     1021 cgcttgccac atccatcaag gaaaaatgat ctgttaaatg aagccctcct caggcgggct
     1081 gaatggatac tgtgggcttc ttaaagacaa accgcagtac ccagggcagc ttgctagcat
     1141 gctggatcag gacctcattt cacgttagtc acaacgcccc ctccccttgt atcttactga
     1201 cccgctatgc taaaaataaa ctttcttcct ctggagtcta aaaaaaaaaa aaaaaaa
//
