LOCUS       NM_025769               1128 bp    mRNA    linear   ROD 10-FEB-2025
DEFINITION  Mus musculus calaxin (Clxn), mRNA.
ACCESSION   NM_025769
VERSION     NM_025769.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1128)
  AUTHORS   Hjeij,R., Aprea,I., Poeta,M., Nothe-Menchen,T., Bracht,D.,
            Raidt,J., Honecker,B.I., Dougherty,G.W., Olbrich,H., Schwartz,O.,
            Keller,U., Nusse,H., Diderich,K.E.M., Vogelberg,C., Santamaria,F.
            and Omran,H.
  TITLE     Pathogenic variants in CLXN encoding the outer dynein arm
            docking-associated calcium-binding protein calaxin cause primary
            ciliary dyskinesia
  JOURNAL   Genet Med 25 (5), 100798 (2023)
   PUBMED   36727596
  REMARK    GeneRIF: Pathogenic variants in CLXN encoding the outer dynein arm
            docking-associated calcium-binding protein calaxin cause primary
            ciliary dyskinesia.
REFERENCE   2  (bases 1 to 1128)
  AUTHORS   Sasaki,K., Shiba,K., Nakamura,A., Kawano,N., Satouh,Y.,
            Yamaguchi,H., Morikawa,M., Shibata,D., Yanase,R., Jokura,K.,
            Nomura,M., Miyado,M., Takada,S., Ueno,H., Nonaka,S., Baba,T.,
            Ikawa,M., Kikkawa,M., Miyado,K. and Inaba,K.
  TITLE     Calaxin is required for cilia-driven determination of vertebrate
            laterality
  JOURNAL   Commun Biol 2, 226 (2019)
   PUBMED   31240264
  REMARK    GeneRIF: Mice with a null mutation in Efcab1 display typical
            phenotypes of primary ciliary dyskinesia, including hydrocephalus,
            situs inversus, and abnormal motility of trachea cilia and sperm
            flagella. Strikingly, both males and females are viable and
            fertile, indicating that calaxin is not essential for fertilization
            in mice. The 9 + 2 axonemal structures of epithelial multicilia and
            sperm flagella are normal.
            Erratum:[Commun Biol. 2019 Jul 4;2:254. doi:
            10.1038/s42003-019-0512-5.. PMID: 31286071]
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 1128)
  AUTHORS   Stauber,M., Weidemann,M., Dittrich-Breiholz,O., Lobschat,K.,
            Alten,L., Mai,M., Beckers,A., Kracht,M. and Gossler,A.
  TITLE     Identification of FOXJ1 effectors during ciliogenesis in the foetal
            respiratory epithelium and embryonic left-right organiser of the
            mouse
  JOURNAL   Dev Biol 423 (2), 170-188 (2017)
   PUBMED   27914912
REFERENCE   4  (bases 1 to 1128)
  AUTHORS   Hoffman,B.G., Zavaglia,B., Witzsche,J., Ruiz de Algara,T.,
            Beach,M., Hoodless,P.A., Jones,S.J., Marra,M.A. and Helgason,C.D.
  TITLE     Identification of transcripts with enriched expression in the
            developing and adult pancreas
  JOURNAL   Genome Biol 9 (6), R99 (2008)
   PUBMED   18554416
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from AC154606.2.
            
            On Apr 29, 2007 this sequence version replaced NM_025769.2.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK017267.1, BY717074.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849384, SAMN01164131
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-167               AC154606.2         114164-114330
            168-326             AC154606.2         124344-124502
            327-431             AC154606.2         125161-125265
            432-583             AC154606.2         127813-127964
            584-688             AC154606.2         128299-128403
            689-1128            AC154606.2         131601-132040
FEATURES             Location/Qualifiers
     source          1..1128
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="16"
                     /map="16 10.09 cM"
     gene            1..1128
                     /gene="Clxn"
                     /gene_synonym="4930449A16Rik; 5430404L10Rik; Efcab1"
                     /note="calaxin"
                     /db_xref="GeneID:66793"
                     /db_xref="MGI:MGI:1914043"
     exon            1..167
                     /gene="Clxn"
                     /gene_synonym="4930449A16Rik; 5430404L10Rik; Efcab1"
                     /inference="alignment:Splign:2.1.0"
     CDS             110..748
                     /gene="Clxn"
                     /gene_synonym="4930449A16Rik; 5430404L10Rik; Efcab1"
                     /note="EF-hand calcium-binding domain-containing protein
                     1; EF-hand calcium binding domain 1"
                     /codon_start=1
                     /product="calaxin"
                     /protein_id="NP_080045.1"
                     /db_xref="CCDS:CCDS27975.1"
                     /db_xref="GeneID:66793"
                     /db_xref="MGI:MGI:1914043"
                     /translation="MNRKKLQKLTDTLTKNCKHFDKFEVKCLITLFYNLVGDVAERPG
                     VVTGLDRNVFRNILHVTFGMTDDMIMDRVFRGFDKDNDGCISVSEWIHGLSLFLRGTL
                     DEKMKYCFEVFDLNGDGFISKEEMFHMLKNSLLKQPSEEDPDEGIKDLVEITLKKMDH
                     DHDGKLSFVDYEKAVREENLLLEAFGPCLPDPKSQMEFEAQVFKDPNEFNDM"
     exon            168..326
                     /gene="Clxn"
                     /gene_synonym="4930449A16Rik; 5430404L10Rik; Efcab1"
                     /inference="alignment:Splign:2.1.0"
     exon            327..431
                     /gene="Clxn"
                     /gene_synonym="4930449A16Rik; 5430404L10Rik; Efcab1"
                     /inference="alignment:Splign:2.1.0"
     exon            432..583
                     /gene="Clxn"
                     /gene_synonym="4930449A16Rik; 5430404L10Rik; Efcab1"
                     /inference="alignment:Splign:2.1.0"
     exon            584..688
                     /gene="Clxn"
                     /gene_synonym="4930449A16Rik; 5430404L10Rik; Efcab1"
                     /inference="alignment:Splign:2.1.0"
     exon            689..1128
                     /gene="Clxn"
                     /gene_synonym="4930449A16Rik; 5430404L10Rik; Efcab1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      955..960
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Clxn"
                     /gene_synonym="4930449A16Rik; 5430404L10Rik; Efcab1"
                     /note="hexamer: CATAAA"
     polyA_site      977
                     /gene="Clxn"
                     /gene_synonym="4930449A16Rik; 5430404L10Rik; Efcab1"
                     /note="major polyA site"
ORIGIN      
        1 gagccaagct ggctgcacca agctccctca gcctggggct ccggagccgg agcgtcctgg
       61 cactctgctc cagatgacac tctaagatct aatattatta acacccatca tgaacagaaa
      121 gaagctgcag aagttgacgg acaccttaac taaaaattgc aagcactttg acaaatttga
      181 ggtgaaatgt cttataacac ttttctataa tttggtggga gacgtagcag agaggccagg
      241 agtagtcacc ggactagatc gtaatgtatt tcggaacatc ttgcatgtga catttggaat
      301 gacagatgac atgattatgg acagagtatt tcgaggcttt gataaagaca atgatggctg
      361 tataagcgta tcagagtgga ttcatggatt atcactgttt cttcgaggga ctttagatga
      421 aaaaatgaaa tattgctttg aagtgtttga tctgaatggt gatggcttca tttcaaagga
      481 ggagatgttc cacatgctga agaacagcct tctcaagcag ccctctgagg aggaccctga
      541 tgaagggatt aaagatttgg ttgaaattac acttaagaaa atggaccatg accatgatgg
      601 gaagctgtct tttgtagact atgagaaggc tgtgagagaa gagaatctcc tgctggaagc
      661 ctttgggcct tgcctgcctg atccaaagag ccagatggaa tttgaagccc aagtattcaa
      721 agatccaaat gaatttaatg atatgtgaat tcctaaatgt actgtgagta agtacaaaag
      781 gagccacatt aagttcatct gttcttctca gctcagaaga tgccatgtgg tgagatgcag
      841 ttcacttgta tgtattttag ttcaggacac cctcagatat gctgggtttg gcaagagagg
      901 ttaacattgt taaagatatt tcaccacatt tactctgtta agacatatat gtgtcataaa
      961 tctccagatt aatcaaagcc atttgcaaat gatatagtca tcatttatgg atacaatcta
     1021 tatgtcagaa agtgtttgct tatatatcga aatattgttg aaactatagt ttccttcata
     1081 gcaataagtg gtgaagagag taccaaaaac aaacaaaaca aaacaaac
//
