LOCUS       NM_001003908            6542 bp    mRNA    linear   ROD 21-MAY-2024
DEFINITION  Mus musculus clathrin heavy chain (Cltc), transcript variant 2,
            mRNA.
ACCESSION   NM_001003908 XM_181312
VERSION     NM_001003908.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 6542)
  AUTHORS   Yang,Y., Zhan,X., Zhang,C., Shi,J., Wu,J., Deng,X., Hong,Y., Li,Q.,
            Ge,S., Xu,G. and He,F.
  TITLE     USP25-PKM2-glycolysis axis contributes to ischemia
            reperfusion-induced acute kidney injury by promoting M1-like
            macrophage polarization and proinflammatory response
  JOURNAL   Clin Immunol 251, 109279 (2023)
   PUBMED   36894047
REFERENCE   2  (bases 1 to 6542)
  AUTHORS   Wang,H., Shan,X., Ren,M., Shang,M. and Zhou,C.
  TITLE     Nucleosomes enter cells by clathrin- and caveolin-dependent
            endocytosis
  JOURNAL   Nucleic Acids Res 49 (21), 12306-12319 (2021)
   PUBMED   34865123
  REMARK    GeneRIF: Nucleosomes enter cells by clathrin- and
            caveolin-dependent endocytosis.
REFERENCE   3  (bases 1 to 6542)
  AUTHORS   Zhang,X., Zou,L., Meng,L., Xiong,M., Pan,L., Chen,G., Zheng,Y.,
            Xiong,J., Wang,Z., Duong,D.M., Zhang,Z., Cao,X., Wang,T., Tang,L.,
            Ye,K. and Zhang,Z.
  TITLE     Amphiphysin I cleavage by asparagine endopeptidase leads to tau
            hyperphosphorylation and synaptic dysfunction
  JOURNAL   Elife 10, e65301 (2021)
   PUBMED   34018922
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 6542)
  AUTHORS   Li,K., Zhang,K., Wang,H., Wu,Y., Chen,N., Chen,J., Qiu,C., Cai,P.,
            Li,M., Liang,X. and Su,D.
  TITLE     Hrd1-mediated ACLY ubiquitination alleviate NAFLD in db/db mice
  JOURNAL   Metabolism 114, 154349 (2021)
   PUBMED   32888949
REFERENCE   5  (bases 1 to 6542)
  AUTHORS   Guo,D., Ru,J., Mao,F., Ouyang,H., Ju,R., Wu,K., Liu,Y. and Liu,C.
  TITLE     Ontogenesis of the tear drainage system requires Prickle1-driven
            polarized basement membrane deposition
  JOURNAL   Development 147 (22) (2020)
   PUBMED   33144400
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 6542)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
REFERENCE   7  (bases 1 to 6542)
  AUTHORS   Gitton,Y., Dahmane,N., Baik,S., Ruiz i Altaba,A., Neidhardt,L.,
            Scholze,M., Herrmann,B.G., Kahlem,P., Benkahla,A., Schrinner,S.,
            Yildirimman,R., Herwig,R., Lehrach,H. and Yaspo,M.L.
  CONSRTM   HSA21 expression map initiative
  TITLE     A gene expression map of human chromosome 21 orthologues in the
            mouse
  JOURNAL   Nature 420 (6915), 586-590 (2002)
   PUBMED   12466855
REFERENCE   8  (bases 1 to 6542)
  AUTHORS   Di Paolo,G., Sankaranarayanan,S., Wenk,M.R., Daniell,L.,
            Perucco,E., Caldarone,B.J., Flavell,R., Picciotto,M.R., Ryan,T.A.,
            Cremona,O. and De Camilli,P.
  TITLE     Decreased synaptic vesicle recycling efficiency and cognitive
            deficits in amphiphysin 1 knockout mice
  JOURNAL   Neuron 33 (5), 789-804 (2002)
   PUBMED   11879655
REFERENCE   9  (bases 1 to 6542)
  AUTHORS   Piao,Y., Ko,N.T., Lim,M.K. and Ko,M.S.
  TITLE     Construction of long-transcript enriched cDNA libraries from
            submicrogram amounts of total RNAs by a universal PCR amplification
            method
  JOURNAL   Genome Res 11 (9), 1553-1558 (2001)
   PUBMED   11544199
REFERENCE   10 (bases 1 to 6542)
  AUTHORS   Engqvist-Goldstein,A.E., Kessels,M.M., Chopra,V.S., Hayden,M.R. and
            Drubin,D.G.
  TITLE     An actin-binding protein of the Sla2/Huntingtin interacting protein
            1 family is a novel component of clathrin-coated pits and vesicles
  JOURNAL   J Cell Biol 147 (7), 1503-1518 (1999)
   PUBMED   10613908
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CJ103480.1, BB840649.1, BC079897.1, CF745318.1 and BP758029.1.
            
            On Oct 4, 2017 this sequence version replaced NM_001003908.1.
            
            Transcript Variant: This variant (2) lacks an alternate in-frame
            exon in the 3' coding region, compared to variant 1. It encodes
            isoform 2, which lacks an internal segment and is shorter, compared
            to isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK122193.1, BC079897.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164131, SAMN01164135
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2                 CJ103480.1         1-2
            3-75                BB840649.1         1-73
            76-6219             BC079897.1         1-6144
            6220-6472           CF745318.1         457-709
            6473-6542           BP758029.1         1-70                c
FEATURES             Location/Qualifiers
     source          1..6542
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="11"
                     /map="11 51.82 cM"
     gene            1..6542
                     /gene="Cltc"
                     /gene_synonym="3110065L21Rik; CHC"
                     /note="clathrin heavy chain"
                     /db_xref="GeneID:67300"
                     /db_xref="MGI:MGI:2388633"
     exon            1..320
                     /gene="Cltc"
                     /gene_synonym="3110065L21Rik; CHC"
                     /inference="alignment:Splign:2.1.0"
     CDS             279..5306
                     /gene="Cltc"
                     /gene_synonym="3110065L21Rik; CHC"
                     /note="isoform 2 is encoded by transcript variant 2;
                     clathrin heavy chain 1; clathrin, heavy polypeptide (Hc)"
                     /codon_start=1
                     /product="clathrin heavy chain 1 isoform 2"
                     /protein_id="NP_001003908.1"
                     /db_xref="CCDS:CCDS25204.1"
                     /db_xref="GeneID:67300"
                     /db_xref="MGI:MGI:2388633"
                     /translation="MAQILPIRFQEHLQLQNLGINPANIGFSTLTMESDKFICIREKV
                     GEQAQVVIIDMNDPSNPIRRPISADSAIMNPASKVIALKAGKTLQIFNIEMKSKMKAH
                     TMTDDVTFWKWISLNTVALVTDNAVYHWSMEGESQPVKMFDRHSSLAGCQIINYRTDA
                     KQKWLLLTGISAQQNRVVGAMQLYSVDRKVSQPIEGHAASFAQFKMEGNAEESTLFCF
                     AVRGQAGGKLHIIEVGTPPTGNQPFPKKAVDVFFPPEAQNDFPVAMQISEKHDVVFLI
                     TKYGYIHLYDLETGTCIYMNRISGETIFVTAPHEATAGIIGVNRKGQVLSVCVEEENI
                     IPYITNVLQNPDLALRMAVRNNLAGAEELFARKFNALFAQGNYSEAAKVAANAPKGIL
                     RTPDTIRRFQSVPAQPGQTSPLLQYFGILLDQGQLNKYESLELCRPVLQQGRKQLLEK
                     WLKEDKLECSEELGDLVKSVDPTLALSVYLRANVPNKVIQCFAETGQVQKIVLYAKKV
                     GYTPDWIFLLRNVMRISPDQGQQFAQMLVQDEEPLADITQIVDVFMEYNLIQQCTAFL
                     LDALKNNRPSEGPLQTRLLEMNLMHAPQVADAILGNQMFTHYDRAHIAQLCEKAGLLQ
                     RALEHFTDLYDIKRAVVHTHLLNPEWLVNYFGSLSVEDSLECLRAMLSANIRQNLQIC
                     VQVASKYHEQLSTQSLIELFESFKSFEGLFYFLGSIVNFSQDPDVHFKYIQAACKTGQ
                     IKEVERICRESNCYDPERVKNFLKEAKLTDQLPLIIVCDRFDFVHDLVLYLYRNNLQK
                     YIEIYVQKVNPSRLPVVIGGLLDVDCSEDVIKNLILVVRGQFSTDELVAEVEKRNRLK
                     LLLPWLEARIHEGCEEPATHNALAKIYIDSNNNPERFLRENPYYDSRVVGKYCEKRDP
                     HLACVAYERGQCDLELINVCNENSLFKSLSRYLVRRKDPELWGSVLLESNPYRRPLID
                     QVVQTALSETQDPEEVSVTVKAFMTADLPNELIELLEKIVLDNSVFSEHRNLQNLLIL
                     TAIKADRTRVMEYINRLDNYDAPDIANIAISNELFEEAFAIFRKFDVNTSAVQVLIEH
                     IGNLDRAYEFAERCNEPAVWSQLAKAQLQKGMVKEAIDSYIKADDPSSYMEVVQAANA
                     SGNWEELVKYLQMARKKARESYVETELIFALAKTNRLAELEEFINGPNNAHIQQVGDR
                     CYDEKMYDAAKLLYNNVSNFGRLASTLVHLGEYQAAVDGARKANSTRTWKEVCFACVD
                     GKEFRLAQMCGLHIVVHADELEELINYYQDRGYFEELITMLEAALGLERAHMGMFTEL
                     AILYSKFKPQKMREHLELFWSRVNIPKVLRAAEQAHLWAELVFLYDKYEEYDNAIITM
                     MNHPTDAWKEGQFKDIITKVANVELYYKAIQFYLEFKPLLLNDLLMVLSPRLDHTRAV
                     NYFSKVKQLPLVKPYLRSVQNHNNKSVNESLNNLFITEEDYQALRTSIDAYDNFDNIS
                     LAQRLEKHELIEFRRIAAYLFKGNNRWKQSVELCKKDSLYKDAMQYASESKDTELAEE
                     LLQWFLQEEKRECFGACLFTCYDLLRPDVVLETAWRHNIMDFAMPYFIQVMKEYLTKV
                     DKLDASESLRKEEEQATETQPIVYGQPQLMLTAGPSVAVPPQAPFGYGYTAPPYGQPQ
                     PGFGYSM"
     exon            321..528
                     /gene="Cltc"
                     /gene_synonym="3110065L21Rik; CHC"
                     /inference="alignment:Splign:2.1.0"
     exon            529..797
                     /gene="Cltc"
                     /gene_synonym="3110065L21Rik; CHC"
                     /inference="alignment:Splign:2.1.0"
     exon            798..959
                     /gene="Cltc"
                     /gene_synonym="3110065L21Rik; CHC"
                     /inference="alignment:Splign:2.1.0"
     exon            960..1073
                     /gene="Cltc"
                     /gene_synonym="3110065L21Rik; CHC"
                     /inference="alignment:Splign:2.1.0"
     exon            1074..1247
                     /gene="Cltc"
                     /gene_synonym="3110065L21Rik; CHC"
                     /inference="alignment:Splign:2.1.0"
     exon            1248..1445
                     /gene="Cltc"
                     /gene_synonym="3110065L21Rik; CHC"
                     /inference="alignment:Splign:2.1.0"
     exon            1446..1646
                     /gene="Cltc"
                     /gene_synonym="3110065L21Rik; CHC"
                     /inference="alignment:Splign:2.1.0"
     exon            1647..1799
                     /gene="Cltc"
                     /gene_synonym="3110065L21Rik; CHC"
                     /inference="alignment:Splign:2.1.0"
     exon            1800..1922
                     /gene="Cltc"
                     /gene_synonym="3110065L21Rik; CHC"
                     /inference="alignment:Splign:2.1.0"
     exon            1923..2060
                     /gene="Cltc"
                     /gene_synonym="3110065L21Rik; CHC"
                     /inference="alignment:Splign:2.1.0"
     exon            2061..2225
                     /gene="Cltc"
                     /gene_synonym="3110065L21Rik; CHC"
                     /inference="alignment:Splign:2.1.0"
     exon            2226..2406
                     /gene="Cltc"
                     /gene_synonym="3110065L21Rik; CHC"
                     /inference="alignment:Splign:2.1.0"
     exon            2407..2570
                     /gene="Cltc"
                     /gene_synonym="3110065L21Rik; CHC"
                     /inference="alignment:Splign:2.1.0"
     exon            2571..2696
                     /gene="Cltc"
                     /gene_synonym="3110065L21Rik; CHC"
                     /inference="alignment:Splign:2.1.0"
     exon            2697..2839
                     /gene="Cltc"
                     /gene_synonym="3110065L21Rik; CHC"
                     /inference="alignment:Splign:2.1.0"
     exon            2840..3074
                     /gene="Cltc"
                     /gene_synonym="3110065L21Rik; CHC"
                     /inference="alignment:Splign:2.1.0"
     exon            3075..3197
                     /gene="Cltc"
                     /gene_synonym="3110065L21Rik; CHC"
                     /inference="alignment:Splign:2.1.0"
     exon            3198..3343
                     /gene="Cltc"
                     /gene_synonym="3110065L21Rik; CHC"
                     /inference="alignment:Splign:2.1.0"
     exon            3344..3527
                     /gene="Cltc"
                     /gene_synonym="3110065L21Rik; CHC"
                     /inference="alignment:Splign:2.1.0"
     exon            3528..3720
                     /gene="Cltc"
                     /gene_synonym="3110065L21Rik; CHC"
                     /inference="alignment:Splign:2.1.0"
     exon            3721..3878
                     /gene="Cltc"
                     /gene_synonym="3110065L21Rik; CHC"
                     /inference="alignment:Splign:2.1.0"
     exon            3879..4043
                     /gene="Cltc"
                     /gene_synonym="3110065L21Rik; CHC"
                     /inference="alignment:Splign:2.1.0"
     exon            4044..4151
                     /gene="Cltc"
                     /gene_synonym="3110065L21Rik; CHC"
                     /inference="alignment:Splign:2.1.0"
     exon            4152..4319
                     /gene="Cltc"
                     /gene_synonym="3110065L21Rik; CHC"
                     /inference="alignment:Splign:2.1.0"
     exon            4320..4469
                     /gene="Cltc"
                     /gene_synonym="3110065L21Rik; CHC"
                     /inference="alignment:Splign:2.1.0"
     exon            4470..4601
                     /gene="Cltc"
                     /gene_synonym="3110065L21Rik; CHC"
                     /inference="alignment:Splign:2.1.0"
     exon            4602..4712
                     /gene="Cltc"
                     /gene_synonym="3110065L21Rik; CHC"
                     /inference="alignment:Splign:2.1.0"
     exon            4713..4883
                     /gene="Cltc"
                     /gene_synonym="3110065L21Rik; CHC"
                     /inference="alignment:Splign:2.1.0"
     exon            4884..5105
                     /gene="Cltc"
                     /gene_synonym="3110065L21Rik; CHC"
                     /inference="alignment:Splign:2.1.0"
     exon            5106..5181
                     /gene="Cltc"
                     /gene_synonym="3110065L21Rik; CHC"
                     /inference="alignment:Splign:2.1.0"
     exon            5182..6520
                     /gene="Cltc"
                     /gene_synonym="3110065L21Rik; CHC"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6194..6199
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Cltc"
                     /gene_synonym="3110065L21Rik; CHC"
                     /note="hexamer: AATAAA"
     polyA_site      6219
                     /gene="Cltc"
                     /gene_synonym="3110065L21Rik; CHC"
                     /note="major polyA site"
     regulatory      6244..6249
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Cltc"
                     /gene_synonym="3110065L21Rik; CHC"
                     /note="hexamer: AATACA"
     polyA_site      6262
                     /gene="Cltc"
                     /gene_synonym="3110065L21Rik; CHC"
     regulatory      6499..6504
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Cltc"
                     /gene_synonym="3110065L21Rik; CHC"
                     /note="hexamer: AATAAA"
     polyA_site      6520
                     /gene="Cltc"
                     /gene_synonym="3110065L21Rik; CHC"
ORIGIN      
        1 gggcggagtt ctgcctcggg atccttccgc agggctcagc cgtttccgga gtctgcgctg
       61 ctcccggttc cgccattgcg gctctcccgg cccctggagc ctccgccccc gacccaagct
      121 ctctcgtctg cctgccagtt ccctgcgtcc ccggagagta tccctctgag cccagcctcc
      181 cctccccttc cccgtctcct cctctccctt ggagagcccg ggcaggcact gccctgcagc
      241 cccagtgaca ggaggagacc atactccccg acagcgccat ggcccagatt ctgcccattc
      301 gctttcagga gcatctccag ctccagaacc tgggtatcaa cccagcaaac attggcttca
      361 gtaccttgac tatggagtca gacaaattca tctgcattag agaaaaagtg ggagagcagg
      421 cccaggtggt aatcattgat atgaatgatc caagtaatcc aattcgaaga ccaatctcag
      481 cagacagtgc catcatgaat cctgccagca aagtgattgc actgaaagct ggcaaaactc
      541 ttcagatatt taacattgaa atgaaaagta aaatgaaggc ccataccatg actgatgatg
      601 tcaccttctg gaaatggatc tctttgaata cggttgctct tgttacggat aatgcagttt
      661 atcactggag tatggaagga gagtctcagc cagtgaaaat gtttgatcgt cattctagcc
      721 tcgcaggatg ccagatcatc aattaccgta cagatgcaaa gcagaaatgg ttgcttctca
      781 ctggcatatc tgcacagcaa aatcgtgtgg ttggagctat gcagctgtat tctgtagata
      841 ggaaagtttc tcagcctatt gaagggcatg cagctagttt tgcacaattt aagatggaag
      901 gaaatgcaga agaatcaaca ttattttgtt ttgctgtgcg gggtcaggca ggaggaaagt
      961 tacatatcat tgaagttggt accccaccca cagggaatca acccttccca aagaaggcag
     1021 tggatgtttt ctttcctcca gaagcacaaa atgactttcc tgttgcaatg cagatcagtg
     1081 aaaagcatga tgtggtgttc ttgattacca agtatggtta tattcacctc tatgatcttg
     1141 agactggcac gtgcatctac atgaacagaa tcagcggaga aaccatcttc gtcactgcac
     1201 ctcatgaagc cacagctgga ataataggag tcaacagaaa gggacaggtc ctgtcagttt
     1261 gtgtggaaga ggagaacata attccttata tcaccaatgt cctacaaaat cctgacttgg
     1321 ctctgagaat ggctgtgcgg aacaacttag ctggtgctga agaactcttt gcccggaaat
     1381 ttaatgctct cttcgcccag ggaaactact cagaggctgc aaaggtggct gctaatgctc
     1441 caaagggaat ccttcgtact ccagatacca tccgtcgatt ccagagtgtc ccagctcagc
     1501 caggtcaaac gtcacctcta cttcagtatt tcggaatcct tttggaccag ggccagctga
     1561 acaaatacga gtccttagag ttatgtagac ctgtacttca gcaagggcgg aaacagcttt
     1621 tggagaaatg gctgaaagaa gataagctgg aatgttctga agaactgggt gatctcgtta
     1681 aatctgtgga cccaacatta gcactgagtg tttacctcag ggccaatgta cctaataaag
     1741 tcatccagtg ctttgcagag acaggccaag tccagaagat tgttttatat gctaaaaaag
     1801 ttggctatac tccagactgg atctttctcc tgagaaatgt catgcggatc agtccagatc
     1861 aaggacagca gtttgctcaa atgttagttc aggatgaaga gcctcttgct gatatcaccc
     1921 agattgtgga tgtctttatg gaatacaatc taatccaaca atgtactgcc ttcttacttg
     1981 atgctttgaa gaataatcgt ccctctgaag gtcctttgca gacgcgattg cttgagatga
     2041 accttatgca cgcacctcaa gttgcagatg ccattctcgg gaatcagatg ttcacccact
     2101 atgaccgggc tcatatcgcc cagctgtgtg agaaggcggg cctgctgcag cgtgcattag
     2161 aacacttcac cgatttatat gacataaaac gtgcagttgt tcatacccat cttcttaacc
     2221 ctgagtggtt agtgaattac tttgggtcct tatcagttga agattcccta gaatgcctca
     2281 gagccatgct gtcagctaat attcgtcaaa atctacagat ctgtgttcag gtggcttcta
     2341 aatatcacga acagctgtca actcagtctc tgattgagct ctttgaatca ttcaagagtt
     2401 ttgaaggtct cttttatttt ctggggtcca ttgttaactt tagtcaggac ccagatgtgc
     2461 actttaaata tattcaggcg gcttgcaaga ctgggcaaat caaagaagtg gaaagaatct
     2521 gcagagaaag caactgctat gatcccgagc gagtcaagaa ttttctcaag gaggcaaaac
     2581 taacagatca gttgccactt atcattgtct gtgatcggtt tgactttgtc catgacctgg
     2641 tgctgtattt atatagaaac aatcttcaga agtatataga gatatatgtc cagaaggtaa
     2701 atccaagtcg acttcctgtg gttattggag gattgctgga tgttgactgc tctgaagatg
     2761 tcattaaaaa tttgattctt gttgtgagag gtcaattctc tactgatgag cttgttgctg
     2821 aagtcgagaa aagaaacaga ttgaagctgc ttcttccttg gcttgaggcc agaattcatg
     2881 agggctgtga ggagcctgct actcacaatg cacttgccaa aatatacatt gacagtaaca
     2941 acaacccaga gagatttctt cgtgaaaacc cttattatga cagtcgtgtt gttgggaagt
     3001 actgtgagaa gagagacccc catctggctt gtgttgctta tgaacgtggc cagtgtgacc
     3061 tggagctcat caatgtttgc aatgagaatt ccctcttcaa aagcctttct cgctacttag
     3121 tccgtcgaaa ggatccagaa ttgtggggta gtgtgctact ggaaagcaat ccatacagaa
     3181 gaccgttaat tgaccaggtt gtacagacag ccttgtctga aactcaggac ccagaagaag
     3241 tgtcagtaac tgtgaaggct tttatgacag cagatcttcc taatgagctc attgaactgc
     3301 tggagaaaat cgttcttgat aactccgtat tcagtgagca caggaatctg caaaatctcc
     3361 tcatcctcac tgcaattaag gctgaccgaa cacgtgttat ggagtatatt aaccgcctgg
     3421 ataattatga tgctccagat attgccaaca ttgccatcag caatgagctg tttgaagagg
     3481 catttgctat tttccggaag tttgatgtca atacttcagc agtgcaggtg ttgattgaac
     3541 atattggaaa cctggatcgt gcatatgagt ttgctgaacg ctgcaatgag cctgctgttt
     3601 ggagtcaact tgcaaaagct cagttgcaga aaggaatggt gaaggaagcc attgattctt
     3661 acatcaaagc agatgatcct tcatcttaca tggaagttgt ccaggctgcc aatgccagcg
     3721 gaaactggga agaattggtg aagtatttgc agatggcacg caagaaggct cgagagtcct
     3781 atgtggagac agaattgata tttgcactgg ctaaaacaaa ccgccttgca gaattagaag
     3841 agttcatcaa tgggccaaat aatgctcata ttcagcaagt tggtgaccgt tgttatgatg
     3901 aaaaaatgta tgatgctgct aagttgttgt acaataatgt ttctaacttt ggacgcttgg
     3961 catctaccct tgttcacctt ggcgagtatc aggcagcagt tgatggtgcc agaaaagcta
     4021 atagtactcg aacatggaaa gaggtctgct ttgcctgtgt agatgggaaa gagttccgcc
     4081 ttgctcagat gtgtggactc catattgtgg tgcatgcaga tgaattagag gagcttatca
     4141 actactatca ggatcgtgga tattttgaag aactcatcac catgttggag gcagccttgg
     4201 gactggagcg agctcacatg ggaatgttca cagagctagc tattttgtat tctaaattca
     4261 agccacagaa aatgagagaa cacctggagt tgttctggtc gagagtgaat attcctaagg
     4321 tgctgagagc tgcagaacag gctcatctct gggcagagct ggtgtttctg tatgacaagt
     4381 acgaagagta tgacaatgcc atcattacca tgatgaatca ccccaccgat gcatggaagg
     4441 aagggcagtt caaagacatc atcaccaagg tggctaatgt ggaactgtac tacaaagcaa
     4501 tccagttcta cttagaattc aagcctttgt tgttaaatga cttgctcatg gtgctgtctc
     4561 cacggttgga tcacactcgt gcagtcaatt atttcagcaa ggtaaaacag ctaccactgg
     4621 tgaaaccata tttacgctca gtgcagaacc acaacaacaa atctgtgaat gaatcactga
     4681 acaacctctt tattactgaa gaagattacc aggctctgcg aacatcaata gatgcttatg
     4741 acaactttga caacatctca cttgctcagc gtttggaaaa acacgaactc attgaattca
     4801 gaagaattgc tgcttatctc ttcaaaggca acaatcgctg gaaacagagt gtagagttgt
     4861 gcaagaaaga cagcctttac aaggatgcaa tgcagtatgc ctctgaatct aaagacacag
     4921 aattggctga agagctccta cagtggtttt tgcaggaaga aaaaagagaa tgctttggag
     4981 cttgtctctt tacctgttat gatctcttaa ggccagatgt cgtcctggaa accgcatgga
     5041 gacataatat catggatttc gccatgccct atttcatcca ggtcatgaag gaatatttga
     5101 ccaaggtgga taaattagat gcttcggagt cgctgagaaa agaagaagaa caagctacag
     5161 agacacagcc cattgtttat ggtcagcccc agctgatgct aacagcagga cccagtgtgg
     5221 cagtccctcc ccaggcacct tttggttatg gttatactgc accaccgtat gggcagccac
     5281 agcctggctt tgggtacagc atgtgagatg gaagctgatc ctgtagtctc ctattttcat
     5341 actgaagcat cctctaccca cttctcagtt tataaacggg gaaacaggca acgtgttctt
     5401 gtaaccttta cttcatgaag gactactttt ttgttctaac tatagacttg ggtcgcctat
     5461 gttaaaactt tattttcaca ttccgcatca ttagaccttc ttttcaaagg ggaatagtat
     5521 tcatgtgggc ttttttctcc ccctcattct ttgaagaact gcttactatt caatctattg
     5581 tgaagaacct gatttgcact ctgtagtgtt taaagaaaca aagaaactct aatattgaat
     5641 ctcttaaatt tagtgtatgt aaacagcttt cactatgtat tgtctaaatg catttaaatc
     5701 tcttttattc aaagaaaagc taaggtgaaa atactggtat gtgaccatgc aagactgtca
     5761 atgccgacaa agacaacact aatcagcata tcctatattg gatcacagtg ctctccaaat
     5821 tatttgaaaa atgcatacag acaacttgcc tgattgtttt tttttttttt taatgagtat
     5881 aaaaggccct ttcacctatg caggtttccc cattatgcat atagaaaatg ctagtgtgtg
     5941 ttgctcactt catgtgtaac aggtgtcctc atgttgtgct gtatcctgtg ctttttctgt
     6001 gggaccattc cattcaggaa caagaagcac catgattcca cgcgtgtgta tttactaacc
     6061 ctcccttccc tgaggtttgt gtatgttgga tattgtggtg ttttagatca ctgagtgtac
     6121 agaagagaga attcaaacaa gatattgctg ttcttcagtt ttgtttgtgg aatttgaaat
     6181 tactccaatt taaaataaat tactggactg tggaaataac atgtagaatt gcagttttaa
     6241 ttaaatacat ctcaaaccag aatcatggtt ttcatagttt catactggat actgctggag
     6301 cattaccggg agccatagag ggaaattatg aaaaagagat gcattatgtc aattacattc
     6361 ctgtagttat gaaagattat acagcagtca gtgaatagtg gactccttct cacgtttcca
     6421 gcattttaat caagtagcac tccagggctt tatgcatttc acgtaactaa gaaaagtatg
     6481 agtgttacac tgacttgaaa taaaggtata ttttttaaaa aaaaaaaaaa aaaaaaaaaa
     6541 aa
//
