LOCUS       NM_013748               1586 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus cytokine-dependent hematopoietic cell linker (Clnk),
            mRNA.
ACCESSION   NM_013748
VERSION     NM_013748.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1586)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 1586)
  AUTHORS   Xu,M., Cai,C., Sun,X., Chen,W., Li,Q. and Zhou,H.
  TITLE     Clnk plays a role in TNF-alpha-induced cell death in murine
            fibrosarcoma cell line L929
  JOURNAL   Biochem Biophys Res Commun 463 (3), 275-279 (2015)
   PUBMED   26009488
  REMARK    GeneRIF: Clnk is required for TNF-alpha -induced cell death in L929
            cells.
REFERENCE   3  (bases 1 to 1586)
  AUTHORS   Hidano,S., Sasanuma,H., Ohshima,K., Seino,K., Kumar,L., Hayashi,K.,
            Hikida,M., Kurosaki,T., Taniguchi,M., Geha,R.S., Kitamura,D. and
            Goitsuka,R.
  TITLE     Distinct regulatory functions of SLP-76 and MIST in NK cell
            cytotoxicity and IFN-gamma production
  JOURNAL   Int Immunol 20 (3), 345-352 (2008)
   PUBMED   18203684
REFERENCE   4  (bases 1 to 1586)
  AUTHORS   Sasanuma,H., Tatsuno,A., Hidano,S., Ohshima,K., Matsuzaki,Y.,
            Hayashi,K., Lowell,C.A., Kitamura,D. and Goitsuka,R.
  TITLE     Dual function for the adaptor MIST in IFN-gamma production by NK
            and CD4+NKT cells regulated by the Src kinase Fgr
  JOURNAL   Blood 107 (9), 3647-3655 (2006)
   PUBMED   16439675
  REMARK    GeneRIF: interaction of MIST with Fgr, mediated by the C-terminal
            proline-rich region of MIST and the SH3 domain of Fgr, was required
            for the suppression of NK-cell receptor-induced IFN-gamma
            production
REFERENCE   5  (bases 1 to 1586)
  AUTHORS   Sasanuma,H., Tatsuno,A., Tsuji,K., Hidano,S., Morita,S.,
            Kitamura,T., Kubo,M., Kitamura,D. and Goitsuka,R.
  TITLE     Transcriptional regulation of SLP-76 family hematopoietic cell
            adaptor MIST/Clnk by STAT5
  JOURNAL   Biochem Biophys Res Commun 321 (1), 145-153 (2004)
   PUBMED   15358227
  REMARK    GeneRIF: STAT5 is involved in cytokine-mediated up-regulation of
            MIST gene expression, in collaboration with other lineage-specific
            transcription factors that promote basal MIST expression in mast
            cells.
REFERENCE   6  (bases 1 to 1586)
  AUTHORS   Utting,O., Sedgmen,B.J., Watts,T.H., Shi,X., Rottapel,R.,
            Iulianella,A., Lohnes,D. and Veillette,A.
  TITLE     Immune functions in mice lacking Clnk, an SLP-76-related adaptor
            expressed in a subset of immune cells
  JOURNAL   Mol Cell Biol 24 (13), 6067-6075 (2004)
   PUBMED   15199160
  REMARK    GeneRIF: Clnk is dispensable for normal differentiation and
            function of T cells, mast cells, and NK cells. Hence, unlike its
            relatives, Clnk is not essential for normal immune functions
REFERENCE   7  (bases 1 to 1586)
  AUTHORS   Fujii,Y., Wakahara,S., Nakao,T., Hara,T., Ohtake,H., Komurasaki,T.,
            Kitamura,K., Tatsuno,A., Fujiwara,N., Hozumi,N., Ra,C., Kitamura,D.
            and Goitsuka,R.
  TITLE     Targeting of MIST to Src-family kinases via SKAP55-SLAP-130 adaptor
            complex in mast cells
  JOURNAL   FEBS Lett 540 (1-3), 111-116 (2003)
   PUBMED   12681493
REFERENCE   8  (bases 1 to 1586)
  AUTHORS   Hurle,B., Lane,K., Kenney,J., Tarantino,L.M., Bucan,M.,
            Brownstein,B.H. and Ornitz,D.M.
  TITLE     Physical mapping of the mouse tilted locus identifies an
            association between human deafness loci DFNA6/14 and vestibular
            system development
  JOURNAL   Genomics 77 (3), 189-199 (2001)
   PUBMED   11597144
REFERENCE   9  (bases 1 to 1586)
  AUTHORS   Goitsuka,R., Kanazashi,H., Sasanuma,H., Fujimura,Y., Hidaka,Y.,
            Tatsuno,A., Ra,C., Hayashi,K. and Kitamura,D.
  TITLE     A BASH/SLP-76-related adaptor protein MIST/Clnk involved in IgE
            receptor-mediated mast cell degranulation
  JOURNAL   Int Immunol 12 (4), 573-580 (2000)
   PUBMED   10744659
REFERENCE   10 (bases 1 to 1586)
  AUTHORS   Cao,M.Y., Davidson,D., Yu,J., Latour,S. and Veillette,A.
  TITLE     Clnk, a novel SLP-76-related adaptor molecule expressed in
            cytokine-stimulated hemopoietic cells
  JOURNAL   J Exp Med 190 (10), 1527-1534 (1999)
   PUBMED   10562326
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from AF187819.1.
            
            On Jun 3, 2007 this sequence version replaced NM_013748.2.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF187819.1, BC125639.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..1586
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="5"
                     /map="5 20.81 cM"
     gene            1..1586
                     /gene="Clnk"
                     /gene_synonym="MIST"
                     /note="cytokine-dependent hematopoietic cell linker"
                     /db_xref="GeneID:27278"
                     /db_xref="MGI:MGI:1351468"
     exon            1..92
                     /gene="Clnk"
                     /gene_synonym="MIST"
                     /inference="alignment:Splign:2.1.0"
     exon            93..145
                     /gene="Clnk"
                     /gene_synonym="MIST"
                     /inference="alignment:Splign:2.1.0"
     CDS             135..1442
                     /gene="Clnk"
                     /gene_synonym="MIST"
                     /note="mast cell immunoreceptor signal transducer"
                     /codon_start=1
                     /product="cytokine-dependent hematopoietic cell linker"
                     /protein_id="NP_038776.3"
                     /db_xref="CCDS:CCDS51486.1"
                     /db_xref="GeneID:27278"
                     /db_xref="MGI:MGI:1351468"
                     /translation="MTSQGNKRTTKEGFGDLRFQNVSLLKNRSWPSLSSAKGRCRAVL
                     EPLPDHRRNLAGVPGGEKCNSNNDYEDPEFQLLKAWPSMKILPARPIQESEYADTRYF
                     QDTMEAPLLLPPKASVSTERQTRDVRMTHLEEVDKPTFKDVRSQRFKGFKYTKINKTP
                     LPPPRPAITLPKKYQPLPPAPPEESSAYFAPKPTFPEVQRGPRQRSAKDFSRVLGAEE
                     ESHHQTKPESSCPSSNQNTQKSPPAIASSSYMPGKHSIQARDHTGSMQHCPAQRCQAA
                     ASHSPRMLPYENTNSEKPDPTKPDEKDVWQNEWYIGEYSRQAVEDVLMKENKDGTFLV
                     RDCSTKSKAEPYVLVVFYGNKVYNVKIRFLESNQQFALGTGLRGNEMFDSVEDIIEHY
                     TYFPILLIDGKDKAARRKQCYLTQPLPLARLLLTQYSSQALHE"
     exon            146..217
                     /gene="Clnk"
                     /gene_synonym="MIST"
                     /inference="alignment:Splign:2.1.0"
     exon            218..246
                     /gene="Clnk"
                     /gene_synonym="MIST"
                     /inference="alignment:Splign:2.1.0"
     exon            247..284
                     /gene="Clnk"
                     /gene_synonym="MIST"
                     /inference="alignment:Splign:2.1.0"
     exon            285..426
                     /gene="Clnk"
                     /gene_synonym="MIST"
                     /inference="alignment:Splign:2.1.0"
     exon            427..536
                     /gene="Clnk"
                     /gene_synonym="MIST"
                     /inference="alignment:Splign:2.1.0"
     exon            537..582
                     /gene="Clnk"
                     /gene_synonym="MIST"
                     /inference="alignment:Splign:2.1.0"
     exon            583..608
                     /gene="Clnk"
                     /gene_synonym="MIST"
                     /inference="alignment:Splign:2.1.0"
     exon            609..628
                     /gene="Clnk"
                     /gene_synonym="MIST"
                     /inference="alignment:Splign:2.1.0"
     exon            629..742
                     /gene="Clnk"
                     /gene_synonym="MIST"
                     /inference="alignment:Splign:2.1.0"
     exon            743..770
                     /gene="Clnk"
                     /gene_synonym="MIST"
                     /inference="alignment:Splign:2.1.0"
     exon            771..789
                     /gene="Clnk"
                     /gene_synonym="MIST"
                     /inference="alignment:Splign:2.1.0"
     exon            790..871
                     /gene="Clnk"
                     /gene_synonym="MIST"
                     /inference="alignment:Splign:2.1.0"
     exon            872..912
                     /gene="Clnk"
                     /gene_synonym="MIST"
                     /inference="alignment:Splign:2.1.0"
     exon            913..1040
                     /gene="Clnk"
                     /gene_synonym="MIST"
                     /inference="alignment:Splign:2.1.0"
     exon            1041..1118
                     /gene="Clnk"
                     /gene_synonym="MIST"
                     /inference="alignment:Splign:2.1.0"
     exon            1119..1274
                     /gene="Clnk"
                     /gene_synonym="MIST"
                     /inference="alignment:Splign:2.1.0"
     exon            1275..1586
                     /gene="Clnk"
                     /gene_synonym="MIST"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 cttacagagt gctccaggat gcgaccgtgg accccctttc caggagctag ccgtctcaac
       61 actgagccct tgactaaagg aagactgagc aggctgagtt gaagatccct ctcctttgcc
      121 aggtgccaag gaccatgacc agccagggca ataaaaggac aacgaaagaa ggattcggtg
      181 atctgagatt ccagaacgtc tctctgctga aaaataggtc atggccaagc ctcagcagtg
      241 ccaaagggcg gtgtcgagcg gttctggaac cacttccgga tcacagaagg aacttggctg
      301 gggtcccagg tggagaaaaa tgcaacagta acaacgacta cgaagatcct gagttccagc
      361 tgctgaaggc atggccatca atgaaaattt taccagccag acctatccag gaatcggaat
      421 acgcagatac acgctatttc caggatacga tggaggctcc ccttctgtta cctcccaagg
      481 cttctgtctc cactgagaga caaaccaggg atgtgaggat gacacatctg gaagaagtgg
      541 acaagcctac cttcaaggat gtcagaagcc aacgctttaa aggattcaaa tacacaaaaa
      601 taaacaagac tcctttgcca cctcctcggc ctgctatcac tctccccaag aagtaccaac
      661 ccttaccccc agcaccacca gaggagagca gtgcatactt cgctccaaag cccacctttc
      721 cagaagtcca gagggggccc aggcagagga gtgcaaaaga cttcagtagg gtccttggag
      781 cagaagaaga atctcaccac cagacaaagc cagaatcttc ttgcccatca tcaaaccaaa
      841 acacacagaa gagtccacct gccattgcca gctcttccta catgccagga aagcacagta
      901 tacaagccag agaccataca ggtagcatgc agcactgtcc tgctcagaga tgccaagctg
      961 cagccagcca cagccctcga atgctgccct atgaaaacac aaactcggag aaacctgacc
     1021 ccacaaagcc tgatgagaag gatgtctggc agaatgaatg gtacattgga gaatacagtc
     1081 gccaggcagt ggaagatgtg ttaatgaaag agaacaagga tggtactttt ttggtccgag
     1141 actgctctac aaaatccaag gcagaaccat atgttttggt ggtgttttat gggaacaagg
     1201 tctacaatgt gaaaatccgt ttcctcgaga gcaatcaaca gtttgccctg ggcacaggac
     1261 tacgaggaaa tgagatgttt gattctgtgg aagacatcat tgaacactac acatattttc
     1321 ccattctgct aatagatggg aaagacaagg ctgcacgcag gaaacagtgc tacctcaccc
     1381 agccactgcc tctcgccagg ctccttctca ctcagtactc cagccaggca cttcatgagt
     1441 aagaagccca gccagatatc cccgcatcag tggcctgggc cttgtctcat tcctggctca
     1501 atggattcag ttcttcttcc atctgcattt atctcaaagt attattttct gtgtcttcaa
     1561 gggatgattt tttgactctg taaaaa
//
